FastQCFastQC Report
Wed 25 May 2016
SRR1780526_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780526_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6235
Sequences flagged as poor quality0
Sequence length76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGCAGTCCGGTGTAGATCTCGGTG80.12830793905372895No Hit
ATACACATCTGACGCTGCCGACGAGCAGTCCGGTGTAGATCTCGGTGGTC80.12830793905372895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAA150.002122674869.99193647
TTTACAC252.2110387E-455.993552
CTTTACA200.00642332952.920731
CCGACGA458.192256E-538.8844120
TACACAT501.523682E-434.9959684
TCTGACG552.668069E-431.81451610
CATCTGA450.00399155231.1075298
GACGCTG450.00399155231.10752913
GCCGACG450.00399155231.10752919
ATCTGAC450.00399155231.1075299
CACATCT604.445041E-429.1633076
TTACACA500.006683100527.9967753
CTGACGC500.006683100527.99677511
ACATCTG500.006683100527.9967757
TGACGCT500.006683100527.99677512
ACACATC750.001637503723.3306475
Produced by FastQC (version 0.11.5)