##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780526_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6235 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.16503608660786 21.0 12.0 31.0 12.0 32.0 2 19.860785886126703 21.0 11.0 27.0 11.0 33.0 3 20.33953488372093 21.0 11.0 31.0 11.0 33.0 4 20.97866880513232 21.0 11.0 31.0 11.0 33.0 5 21.022453889334404 21.0 11.0 31.0 11.0 34.0 6 22.562951082598236 23.0 11.0 34.0 11.0 36.0 7 20.743704891740176 21.0 11.0 31.0 11.0 35.0 8 20.12141138732959 21.0 11.0 28.0 11.0 34.0 9 21.151724137931033 21.0 12.0 31.0 11.0 36.0 10-11 20.28813151563753 21.0 11.0 28.0 11.0 34.0 12-13 20.226784282277464 21.0 11.0 27.5 11.0 34.0 14-15 19.866319165998398 16.5 11.0 27.5 11.0 34.0 16-17 19.285004009623094 11.5 11.0 26.5 11.0 34.0 18-19 19.681716118684843 16.5 11.0 27.5 11.0 34.0 20-21 19.900160384923815 16.0 11.0 27.5 11.0 34.0 22-23 19.243865276663996 16.0 11.0 27.0 10.5 34.0 24-25 19.35501202886929 16.0 11.0 27.0 10.0 34.0 26-27 19.09021651964715 11.0 11.0 26.5 10.0 34.0 28-29 18.455573376102645 11.0 11.0 26.0 11.0 34.0 30-31 18.26655974338412 11.0 11.0 26.0 11.0 34.0 32-33 18.737449879711306 11.0 11.0 26.0 11.0 34.0 34-35 19.028789093825182 11.0 11.0 26.0 11.0 34.0 36-37 18.688853247794707 11.0 11.0 26.0 11.0 34.0 38-39 18.307698476343223 11.0 11.0 26.0 11.0 34.0 40-41 18.710024057738572 11.0 11.0 26.0 11.0 34.0 42-43 18.733600641539695 11.0 11.0 26.0 11.0 34.0 44-45 18.60336808340016 11.0 11.0 26.0 11.0 34.0 46-47 18.80954290296712 11.0 11.0 26.0 11.0 34.0 48-49 19.413311948676824 15.5 11.0 26.5 11.0 34.0 50-51 19.495348837209303 20.0 11.0 26.5 11.0 34.0 52-53 19.357417802726545 15.5 11.0 26.0 11.0 34.0 54-55 19.282758620689656 15.5 11.0 26.0 11.0 34.0 56-57 18.743063352044906 15.0 11.0 26.0 11.0 33.0 58-59 18.641539695268648 15.0 11.0 25.5 11.0 33.0 60-61 18.978909382518044 19.0 11.0 26.0 11.0 33.0 62-63 18.50457097032879 15.0 11.0 25.5 11.0 33.0 64-65 18.645629510825984 15.0 11.0 25.5 10.5 33.0 66-67 18.87578187650361 15.0 11.0 25.5 10.5 33.0 68-69 18.780513231756217 19.0 11.0 25.0 10.0 33.0 70-71 18.508740978348037 11.0 11.0 25.0 10.0 33.0 72-73 18.728708901363273 15.0 11.0 25.0 11.0 32.5 74-75 18.164073777064957 11.0 11.0 24.5 10.5 32.0 76 17.586046511627906 11.0 11.0 24.0 10.0 31.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1580218680921455 1101 2 1.3802398230469848 1101 3 3.2226159450581697 1101 4 0.8729935611509454 1101 5 0.023952156618538112 1101 6 -1.8786506141252133 1101 7 -1.3490277715701957 1101 8 1.5299653995209468 1101 9 -1.5219258024066953 1101 10-11 -2.3731891826213385 1101 12-13 0.6540659741424655 1101 14-15 -0.10863530100755625 1101 16-17 -1.2309752615089593 1101 18-19 -2.0284050330472496 1101 20-21 0.22793512480746614 1101 22-23 -2.6812202060492467 1101 24-25 0.628080365284724 1101 26-27 -0.27822421274994724 1101 28-29 -2.3567851967901916 1101 30-31 0.6057070292042113 1101 32-33 -0.1318709892720804 1101 34-35 -4.42543480675193 1101 36-37 -1.2658208413865708 1101 38-39 -3.3806158594508737 1101 40-41 -0.21017703081260208 1101 42-43 0.6922171226928597 1101 44-45 0.09832282462366138 1101 46-47 2.9627359645887594 1101 48-49 -0.1351791479762845 1101 50-51 -0.0017606739950615236 1101 52-53 -0.7266602087570249 1101 54-55 -2.4972357896415573 1101 56-57 -0.3760758835251963 1101 58-59 -2.0867206691779074 1101 60-61 -0.840683244955649 1101 62-63 -1.199360958367702 1101 64-65 -2.652935552640468 1101 66-67 -0.2971744183961178 1101 68-69 -0.5872571359804546 1101 70-71 -1.9740545888261423 1101 72-73 -2.82602930119495 1101 74-75 -0.3468683448910923 1101 76 -1.1062313935730685 1102 1 2.240909568626904 1102 2 2.882913619838426 1102 3 -1.4618760335514551 1102 4 -0.10026847093461555 1102 5 -1.6739088059483045 1102 6 -2.1380089028952654 1102 7 -3.9800438143509425 1102 8 -3.2668260443293207 1102 9 -5.701070187433434 1102 10-11 0.7979338120310686 1102 12-13 -1.2790891060714351 1102 14-15 -3.6367101673177125 1102 16-17 -0.8740233898512031 1102 18-19 -2.365303428769174 1102 20-21 -1.3977333243903942 1102 22-23 -2.1812202060492467 1102 24-25 -1.536358137389069 1102 26-27 -1.445336512215185 1102 28-29 -3.076036533688587 1102 30-31 -0.12958708844284672 1102 32-33 -2.5222453208228828 1102 34-35 -1.406718229211826 1102 36-37 -3.15352137614593 1102 38-39 -1.3632361803064867 1102 40-41 -0.6353107206521713 1102 42-43 -0.515002128644042 1102 44-45 -1.701142416018051 1102 46-47 -0.304643714555624 1102 48-49 -1.4359812870137176 1102 50-51 -0.20496923014479407 1102 52-53 -1.830938283623336 1102 54-55 -2.410337393919633 1102 56-57 -2.817252354113432 1102 58-59 -0.8647955354880672 1102 60-61 -2.7671538331909424 1102 62-63 -0.2956176428596784 1102 64-65 -0.12486068633030811 1102 66-67 -3.7771209424602876 1102 68-69 -1.7918025905259078 1102 70-71 -2.814963679735232 1102 72-73 -1.6442111193767701 1102 74-75 -1.8482052432868166 1102 76 -1.3736110727174538 1103 1 -0.5356055828882447 1103 2 -0.4163288044039959 1103 3 0.7275179058424861 1103 4 2.5323072866411422 1103 5 0.34503058799109 1103 6 -1.1948270847134488 1103 7 -2.7338316931388214 1103 8 2.990749713246437 1103 9 -1.1518277631910117 1103 10-11 0.9797519938492485 1103 12-13 1.8440169545346237 1103 14-15 -2.723831379438927 1103 16-17 -0.9687203595481755 1103 18-19 1.8809086924429437 1103 20-21 1.7708272816702149 1103 22-23 -1.8478868727159146 1103 24-25 -0.5496157131466468 1103 26-27 -2.346851663730341 1103 28-29 -1.6423244124764658 1103 30-31 -1.9837537551095146 1103 32-33 -1.8536847147622737 1103 34-35 -1.0014151989087914 1103 36-37 -2.4508698609944126 1103 38-39 -1.8708119378822445 1103 40-41 -1.368265266106718 1103 42-43 -0.0945475831894953 1103 44-45 -0.22765756753319977 1103 46-47 0.03626537635346594 1103 48-49 -3.750375226407659 1103 50-51 -1.5250449877205519 1103 52-53 0.9645162618312106 1103 54-55 -0.698216181798422 1103 56-57 -3.5255856874467657 1103 58-59 -1.6394167476092818 1103 60-61 -0.9546538331909424 1103 62-63 -1.897890370132405 1103 64-65 -1.0585728075424257 1103 66-67 -2.4589391242784693 1103 68-69 -3.3580904693137867 1103 70-71 -2.8282212554928066 1103 72-73 -1.0551959678616143 1103 74-75 -0.8652506978322698 1103 76 -0.3243686484750299 1104 1 2.398918226635562 1104 2 -0.6841859472611382 1104 3 2.667994096318676 1104 4 1.2287358580697116 1104 5 1.8866972546577543 1104 6 -2.5817318466182115 1104 7 -2.382641216948347 1104 8 -2.080678858182136 1104 9 -0.6815896679529168 1104 10-11 -0.8059622918650362 1104 12-13 0.8767550497727186 1104 14-15 1.1392638586563102 1104 16-17 0.09675583092801432 1104 18-19 0.16067059720485233 1104 20-21 0.16070823405116386 1104 22-23 0.48544646061742114 1104 24-25 1.875979524948594 1104 26-27 -2.4897088065874797 1104 28-29 -1.5976815553336081 1104 30-31 -0.05815851701427732 1104 32-33 -0.2257085242860839 1104 34-35 1.0759657534721612 1104 36-37 2.2902015675770144 1104 38-39 1.010140443070135 1104 40-41 0.4859014005599498 1104 42-43 1.542357178715264 1104 44-45 -2.16218137705701 1104 46-47 1.2624558525439404 1104 48-49 3.1127200116875784 1104 50-51 -0.2482592734348401 1104 52-53 1.6430876904026412 1104 54-55 2.4803552467730086 1104 56-57 1.516080979219904 1104 58-59 2.494511823819291 1104 60-61 -0.6362014522385628 1104 62-63 -1.2847951320371678 1104 64-65 0.789641478171859 1104 66-67 -0.20893912427846928 1104 68-69 -3.039638088361407 1104 70-71 1.0497549349833832 1104 72-73 1.9596849845193347 1104 74-75 0.39963025454868273 1104 76 0.145869446763065 1105 1 -2.0772722495549125 1105 2 -1.4253867754184881 1105 3 -1.4934965869111387 1105 4 0.3384667069309977 1105 5 -0.14228825258862088 1105 6 -0.2546096934091011 1105 7 -3.003759229370708 1105 8 0.8675613074493356 1105 9 -2.7623350095678205 1105 10-11 2.8275780808057718 1105 12-13 3.4916618820708543 1105 14-15 1.915661374184264 1105 16-17 -2.6299522436061444 1105 18-19 0.45880724316758403 1105 20-21 1.0706461222499257 1105 22-23 1.688345011342058 1105 24-25 2.89150747525915 1105 26-27 -0.10047485213613783 1105 28-29 1.4437263121612176 1105 30-31 -0.28176100148632344 1105 32-33 2.5113515171217813 1105 34-35 -1.4561253438363302 1105 36-37 1.85438376219399 1105 38-39 -0.8997974451286233 1105 40-41 2.427930386067196 1105 42-43 -1.197808452754714 1105 44-45 -0.8236720602868246 1105 46-47 -1.409386797559577 1105 48-49 -0.958708559740991 1105 50-51 -1.3393565819234539 1105 52-53 -0.5680924338209614 1105 54-55 -1.5496654571607387 1105 56-57 2.182747645886568 1105 58-59 -0.1276413852904419 1105 60-61 -0.19106687666920408 1105 62-63 0.8901531081284624 1105 64-65 1.583637337385106 1105 66-67 1.7693217452867493 1105 68-69 -2.1959527881543686 1105 70-71 -0.7131850236087516 1105 72-73 -3.8042901707601686 1105 74-75 -0.0744898282670512 1105 76 -0.7174845905040144 1106 1 -0.12727224955491323 1106 2 1.842004528929337 1106 3 0.26085123917581754 1106 4 0.5406406199744751 1106 5 -2.7966360786755793 1106 6 -0.7198270847134474 1106 7 1.8745016401945094 1106 8 -0.62591695342023 1106 9 -2.810161096524343 1106 10-11 -0.5202480061507515 1106 12-13 -1.6768163787987085 1106 14-15 0.2678352872277401 1106 16-17 -0.8353870262148426 1106 18-19 2.4642420257762794 1106 20-21 1.5249939483368777 1106 22-23 0.34377979395075187 1106 24-25 -1.3954490464799783 1106 26-27 0.48648166960299477 1106 28-29 -0.44649107914313646 1106 30-31 -0.0295870884428453 1106 32-33 -1.274518048095608 1106 34-35 0.1569181344245436 1106 36-37 0.5032968056722531 1106 38-39 -1.1791452712155817 1106 40-41 -1.2807652661067195 1106 42-43 -1.269547583189496 1106 44-45 -2.244324234199869 1106 46-47 -0.8887346236465348 1106 48-49 -1.4337085597409924 1106 50-51 0.5041216789461131 1106 52-53 -1.46048373816879 1106 54-55 0.6351171515349137 1106 56-57 -0.49225235411342894 1106 58-59 -0.6102500809426132 1106 60-61 0.48284616680905756 1106 62-63 0.5521096298675943 1106 64-65 -1.2044061408757614 1106 66-67 0.34106087572153143 1106 68-69 -0.5372571359804574 1106 70-71 -1.8490545888261423 1106 72-73 -0.9010293011949493 1106 74-75 1.1972493021677302 1106 76 -0.38270198180836346 1107 1 1.562727750445088 1107 2 0.2720045289293367 1107 3 -1.1491487608241826 1107 4 -2.8893593800255246 1107 5 -3.8066360786755773 1107 6 -1.6198270847134495 1107 7 2.1945016401945097 1107 8 2.1040830465797704 1107 9 -1.5901610965243442 1107 10-11 -0.7802480061507495 1107 12-13 -0.7118163787987086 1107 14-15 -2.112164712772259 1107 16-17 3.139612973785159 1107 18-19 0.20924202577628037 1107 20-21 -0.5050060516631234 1107 22-23 1.5187797939507526 1107 24-25 -1.0354490464799788 1107 26-27 -2.353518330397005 1107 28-29 3.4185089208568655 1107 30-31 2.7304129115571527 1107 32-33 -1.0695180480956097 1107 34-35 1.6569181344245436 1107 36-37 1.848296805672252 1107 38-39 0.21585472878442147 1107 40-41 3.019234733893285 1107 42-43 -0.2195475831894953 1107 44-45 2.140675765800129 1107 46-47 0.02626537635346793 1107 48-49 0.16129144025900644 1107 50-51 -0.19587832105388614 1107 52-53 1.534516261831211 1107 54-55 1.4051171515349132 1107 56-57 1.362747645886568 1107 58-59 -0.23025008094261423 1107 60-61 0.842846166809057 1107 62-63 2.937109629867596 1107 64-65 -2.409406140875763 1107 66-67 -1.2689391242784716 1107 68-69 0.45274286401954456 1107 70-71 -0.5340545888261445 1107 72-73 -1.0260293011949528 1107 74-75 -1.37275069783227 1107 76 -1.4027019818083613 1108 1 -0.6189389162215804 1108 2 1.0420045289293363 1108 3 2.4358512391758183 1108 4 2.5323072866411422 1108 5 -0.32163607867557786 1108 6 -1.1114937513801166 1108 7 -2.025498359805489 1108 8 0.7824163799131014 1108 9 1.806505570142324 1108 10-11 -2.374414672817416 1108 12-13 -0.5101497121320442 1108 14-15 -0.9946647127722592 1108 16-17 -0.5103870262148398 1108 18-19 2.1934086924429472 1108 20-21 -0.020839384996456545 1108 22-23 1.089613127284089 1108 24-25 1.179550953520021 1108 26-27 -0.5968516637303409 1108 28-29 -2.7464910791431336 1108 30-31 -1.6920870884428467 1108 32-33 -0.1245180480956094 1108 34-35 -0.3139151989087914 1108 36-37 0.2991301390055874 1108 38-39 0.0041880621177554644 1108 40-41 -0.5349319327733824 1108 42-43 -2.6570475831894953 1108 44-45 -1.9568242341998676 1108 46-47 -0.9220679569798698 1108 48-49 -3.125375226407659 1108 50-51 -1.3792116543872233 1108 52-53 -0.16048373816878936 1108 54-55 0.864283818201578 1108 56-57 0.39108097921990037 1108 58-59 0.589749919057386 1108 60-61 -1.2254871665242746 1108 62-63 -2.106223703465737 1108 64-65 -1.2460728075424292 1108 66-67 2.2285608757215307 1108 68-69 1.1835761973528776 1108 70-71 -2.3073879221594744 1108 72-73 -1.07602930119495 1108 74-75 -0.3444173644989377 1108 76 -1.282701981808362 1109 1 -1.6681813404640025 1109 2 -0.4807227437979371 1109 3 -3.870966942642365 1109 4 -3.463904834570979 1109 5 -0.7193633514028512 1109 6 -3.0470998119861754 1109 7 0.7017743674672374 1109 8 0.960446682943406 1109 9 -0.6101610965243438 1109 10-11 1.820661084758342 1109 12-13 -0.18818001516234517 1109 14-15 0.9314716508641041 1109 16-17 -0.0785688443966599 1109 18-19 -0.4107579742237206 1109 20-21 -2.693187869844941 1109 22-23 -0.29485656968561 1109 24-25 -1.0590854101163423 1109 26-27 2.2819362150575415 1109 28-29 0.8785089208568664 1109 30-31 0.4158674570117 1109 32-33 -2.3176998662774295 1109 34-35 -2.520354592848186 1109 36-37 -2.1535213761459318 1109 38-39 0.7049456378753298 1109 40-41 -0.9080379933794447 1109 42-43 -2.1286384922804054 1109 44-45 -0.4511424160180475 1109 46-47 -0.9182800781919873 1109 48-49 -1.7314358324682644 1109 50-51 2.0223034971279326 1109 52-53 0.3508798981948438 1109 54-55 1.498753515171277 1109 56-57 -0.9536159904770649 1109 58-59 -0.4329773536698873 1109 60-61 -1.4489720150091223 1109 62-63 2.568018720776685 1109 64-65 1.6478665863969653 1109 66-67 -1.004393669733016 1109 68-69 -0.4281662268895481 1109 70-71 -1.1558727706443221 1109 72-73 1.4921525169868666 1109 74-75 -0.939114334195903 1109 76 0.6718434727370912 1110 1 1.9227277504450875 1110 2 -0.8746621377373316 1110 3 -1.1335932052686246 1110 4 2.712862842196696 1110 5 1.1089194768799793 1110 6 0.9023951375087726 1110 7 -1.8588316931388214 1110 8 -1.9536947311980093 1110 9 0.11206112569787763 1110 10-11 1.1464186605159163 1110 12-13 0.4759613989790701 1110 14-15 -1.7377202683278163 1110 16-17 1.4062796404518245 1110 18-19 3.5059086924429437 1110 20-21 0.4861050594479899 1110 22-23 1.929890905061864 1110 24-25 -0.3343379353688647 1110 26-27 0.48648166960299477 1110 28-29 -0.17704663469869075 1110 30-31 3.3704129115571533 1110 32-33 -1.3050736036511665 1110 34-35 -4.265304087797681 1110 36-37 -0.1661476387721912 1110 38-39 -2.120811937882248 1110 40-41 1.152568067226614 1110 42-43 1.9471190834771726 1110 44-45 1.8973424324668002 1110 46-47 0.7584875985756874 1110 48-49 0.23573588470345186 1110 50-51 3.065232790057223 1110 52-53 1.186738484053432 1110 54-55 0.3295615959793565 1110 56-57 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-0.1593626345282857 2116 74-75 0.7805826355010623 2116 76 0.16174246263608083 2117 1 -0.22012939241205487 2117 2 1.7920045289293363 2117 3 -5.474863046538468 2117 4 0.7049263342601897 2117 5 -5.3037789358184355 2117 6 -3.3912556561420217 2117 7 1.4745016401945108 2117 8 -0.7473455248488037 2117 9 1.4612674749042291 2117 10-11 2.4797519938492485 2117 12-13 -4.266102093084424 2117 14-15 -3.8607361413436863 2117 16-17 -2.331815597643409 2117 18-19 -4.410757974223721 2117 20-21 -1.9107203373774055 2117 22-23 -4.25264877747782 2117 24-25 -1.3383061893371213 2117 26-27 -6.799232616111292 2117 28-29 -0.9072053648574183 2117 30-31 -0.05815851701427732 2117 32-33 -1.67808947666704 2117 34-35 0.9569181344245408 2117 36-37 -1.0431317657563177 2117 38-39 -4.132716699787007 2117 40-41 -3.8950509803924316 2117 42-43 -3.8624047260466376 2117 44-45 -2.3764670913427253 2117 46-47 -2.4280203379322494 2117 48-49 -1.8872799883124216 2117 50-51 -3.7244497496253146 2117 52-53 -4.285483738168789 2117 54-55 2.027974294392056 2117 56-57 -3.960109496970574 2117 58-59 -0.6959643666568986 2117 60-61 -2.802868118905227 2117 62-63 -0.7371760844181203 2117 64-65 -3.2579775694471884 2117 66-67 -0.494653409992754 2117 68-69 1.341314292590976 2117 70-71 0.45451683974528834 2117 72-73 0.031113555947904104 2117 74-75 -0.4813221264037004 2117 76 -1.4255591246655044 2118 1 -0.22012939241205487 2118 2 -3.0651383282135214 2118 3 -0.3320059036813241 2118 4 -3.723645094311241 2118 5 1.124792492752995 2118 6 -0.8198270847134488 2118 7 6.903073068765938 2118 8 1.109797332294054 2118 9 -1.9673039536672015 2118 10-11 5.694037708134964 2118 12-13 2.3767550497727186 2118 14-15 1.282121001513456 2118 16-17 5.31104154521373 2118 18-19 1.0892420257762794 2118 20-21 1.3749939483368792 2118 22-23 -1.395505920334962 2118 24-25 -2.3383061893371213 2118 26-27 6.70076738388871 2118 28-29 3.164223206571151 2118 30-31 2.798984340128584 2118 32-33 3.1076248090472447 2118 34-35 0.6712038487102561 2118 36-37 -1.257417480042033 2118 38-39 1.5815690144987045 2118 40-41 1.0335204481789972 2118 42-43 -0.1481190117609259 2118 44-45 4.266390051514417 2118 46-47 -0.21373462364653406 2118 48-49 2.4698628688304396 2118 50-51 5.132693107517543 2118 52-53 3.2145162618312106 2118 54-55 2.1708314372491984 2118 56-57 -0.6029666398277165 2118 58-59 0.16117849048595545 2118 60-61 1.1971318810947729 2118 62-63 1.048538201296168 2118 64-65 -0.4722632837329037 2118 66-67 2.5767751614358154 2118 68-69 -2.230114278837597 2118 70-71 0.31165969688814243 2118 72-73 0.5311135559479077 2118 74-75 5.875820730739161 2118 76 -1.282701981808362 2119 1 2.722727750445088 2119 2 2.192004528929335 2119 3 0.21085123917581683 2119 4 -1.609359380025527 2119 5 -3.0466360786755793 2119 6 5.980172915286552 2119 7 -4.525498359805489 2119 8 -0.7759169534202321 2119 9 2.689838903475657 2119 10-11 0.17975199384925133 2119 12-13 0.4481836212012915 2119 14-15 2.8678352872277415 2119 16-17 2.3396129737851616 2119 18-19 0.5892420257762794 2119 20-21 6.974993948336881 2119 22-23 2.8187797939507533 2119 24-25 1.6045509535200182 2119 26-27 6.986481669602995 2119 28-29 2.0785089208568657 2119 30-31 1.9704129115571547 2119 32-33 1.2504819519043906 2119 34-35 3.6569181344245436 2119 36-37 -3.8717031943277487 2119 38-39 5.79585472878442 2119 40-41 1.4192347338932834 2119 42-43 5.4804524168105075 2119 44-45 6.380675765800131 2119 46-47 5.086265376353463 2119 48-49 3.841291440259006 2119 50-51 -0.09587832105388827 2119 52-53 5.714516261831211 2119 54-55 2.3851171515349137 2119 56-57 1.982747645886569 2119 58-59 2.7897499190573853 2119 60-61 -0.6171538331909439 2119 62-63 1.3771096298675936 2119 64-65 2.1705938591242386 2119 66-67 -0.10893912427846786 2119 68-69 3.312742864019544 2119 70-71 5.825945411173855 2119 72-73 2.373970698805053 2119 74-75 2.2472493021677273 2119 76 0.5172980181916387 >>END_MODULE >>Per sequence quality scores fail #Quality Count 11 1.0 12 9.0 13 60.0 14 284.0 15 530.0 16 695.0 17 850.0 18 874.0 19 769.0 20 672.0 21 534.0 22 299.0 23 203.0 24 132.0 25 100.0 26 60.0 27 45.0 28 29.0 29 26.0 30 9.0 31 11.0 32 10.0 33 9.0 34 5.0 35 7.0 36 7.0 37 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 4.25531914893617 56.624758220502905 5.996131528046422 33.1237911025145 2 2.1331194867682437 30.216519647153167 22.9831595829992 44.667201283079386 3 1.9406575781876503 53.60064153969527 16.455493183640737 28.003207698476345 4 1.218925421010425 56.1186848436247 13.937449879711309 28.72493985565357 5 1.4595028067361668 56.64795509222134 15.140336808340017 26.752205292702484 6 3.1595829991980753 37.72253408179631 7.201283079390537 51.916599839615074 7 10.681635926222935 43.480352846832396 8.933440256615878 36.904570970328784 8 12.798716920609463 40.577385725741784 11.467522052927025 35.156375300721734 9 11.259021651964716 33.19967923015237 8.80513231756215 46.736166800320774 10-11 8.195669607056937 33.785084202085 20.98636728147554 37.03287890938252 12-13 6.808340016038493 30.825982357658383 21.427425821972733 40.93825180433039 14-15 12.269446672012831 33.223736968724936 16.24699278267843 38.2598235765838 16-17 17.10505212510024 31.30713712910986 16.39935846030473 35.18845228548517 18-19 12.96712109061748 29.198075380914197 15.685645549318364 42.14915797914996 20-21 10.28869286287089 29.863672814755414 16.52766639935846 43.319967923015234 22-23 18.83720930232558 29.430633520449078 13.656776263031276 38.07538091419407 24-25 14.081796311146752 35.98235765838011 12.445870088211707 37.48997594226143 26-27 10.465116279069768 38.099438652766636 17.55412991178829 33.8813151563753 28-29 15.934242181234964 31.07457898957498 21.210906174819566 31.78027265437049 30-31 15.276663993584602 29.390537289494787 23.648757016840417 31.68404170008019 32-33 10.12830793905373 37.6663993584603 20.4971932638332 31.708099438652766 34-35 10.593424218123497 32.53408179631115 20.753809141940657 36.1186848436247 36-37 10.61748195669607 30.352846832397756 24.025661587810745 35.00400962309543 38-39 11.130713712910985 24.570970328789095 27.129109863672813 37.16920609462711 40-41 8.043303929430634 26.519647153167604 25.060144346431436 40.37690457097033 42-43 4.450681635926223 26.174819566960707 29.190056134723335 40.184442662389735 44-45 6.126704089815557 28.748997594226143 30.62550120288693 34.49879711307137 46-47 5.789895749799519 33.07939053728949 24.76343223736969 36.3672814755413 48-49 6.423416198877306 36.10264635124298 18.829190056134724 38.644747393744986 50-51 6.343223736968725 33.05533279871692 18.436246992782678 42.16519647153167 52-53 5.765838011226944 31.90858059342422 19.759422614274257 42.566158781074584 54-55 6.567762630312751 32.41379310344827 18.460304731355254 42.55813953488372 56-57 10.104250200481154 31.355252606255014 16.535685645549318 42.00481154771452 58-59 10.04811547714515 30.561347233360063 16.79230152365678 42.59823576583801 60-61 6.784282277465918 28.396150761828387 17.834803528468324 46.98476343223737 62-63 9.085805934242181 30.4971932638332 15.902165196471532 44.51483560545309 64-65 9.991980753809141 32.30152365677627 13.015236567762631 44.691259021651966 66-67 9.149959903769046 36.84041700080192 10.761828388131516 43.247794707297516 68-69 8.797113071371292 38.604651162790695 10.737770649558943 41.86046511627907 70-71 10.28869286287089 36.5196471531676 11.65196471531676 41.53969526864475 72-73 8.580593424218124 35.43704891740177 11.435445068163592 44.54691259021652 74-75 11.980753809141941 32.52606255012029 12.028869286287089 43.464314354450686 76 11.275060144346432 27.44186046511628 12.654370489174019 48.62870890136327 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 2.5 16 3.5 17 4.0 18 5.5 19 5.5 20 3.0 21 2.0 22 3.5 23 10.5 24 14.0 25 12.0 26 18.0 27 21.0 28 19.0 29 20.0 30 28.5 31 48.5 32 60.0 33 60.0 34 67.5 35 88.0 36 124.5 37 148.0 38 156.0 39 188.5 40 216.5 41 258.0 42 296.0 43 303.5 44 329.5 45 361.5 46 375.0 47 380.5 48 394.5 49 402.5 50 402.0 51 386.0 52 352.0 53 331.0 54 328.0 55 309.0 56 261.5 57 225.5 58 218.0 59 201.5 60 159.5 61 115.0 62 96.0 63 85.5 64 64.5 65 48.0 66 36.0 67 30.0 68 26.0 69 21.0 70 18.5 71 17.0 72 14.0 73 10.5 74 8.0 75 6.0 76 5.0 77 4.0 78 4.5 79 5.0 80 3.0 81 3.0 82 5.0 83 5.0 84 3.0 85 1.0 86 1.0 87 1.0 88 1.0 89 3.0 90 3.5 91 1.0 92 0.0 93 1.5 94 2.5 95 1.5 96 1.0 97 0.5 98 1.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4971932638331997 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 6235.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.74338412189255 #Duplication Level Percentage of deduplicated Percentage of total 1 99.93568097764914 99.67923015236568 2 0.03215951117543013 0.06415396952686447 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.03215951117543013 0.2566158781074579 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAGCAGTCCGGTGTAGATCTCGGTG 8 0.12830793905372895 No Hit ATACACATCTGACGCTGCCGACGAGCAGTCCGGTGTAGATCTCGGTGGTC 8 0.12830793905372895 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.01603849238171612 0.0 11 0.0 0.0 0.0 0.03207698476343224 0.0 12 0.0 0.0 0.0 0.03207698476343224 0.0 13 0.0 0.0 0.0 0.03207698476343224 0.0 14 0.0 0.0 0.0 0.03207698476343224 0.0 15 0.0 0.0 0.0 0.03207698476343224 0.0 16 0.0 0.0 0.0 0.03207698476343224 0.0 17 0.0 0.0 0.0 0.048115477145148355 0.0 18 0.0 0.0 0.0 0.048115477145148355 0.0 19 0.0 0.0 0.0 0.048115477145148355 0.0 20 0.0 0.0 0.0 0.048115477145148355 0.0 21 0.0 0.0 0.0 0.08019246190858059 0.0 22 0.0 0.0 0.0 0.08019246190858059 0.0 23 0.0 0.0 0.0 0.08019246190858059 0.0 24 0.0 0.0 0.0 0.08019246190858059 0.0 25 0.0 0.0 0.0 0.08019246190858059 0.0 26 0.0 0.0 0.0 0.08019246190858059 0.0 27 0.0 0.0 0.0 0.08019246190858059 0.0 28 0.0 0.0 0.0 0.09623095429029671 0.0 29 0.0 0.0 0.0 0.09623095429029671 0.0 30 0.0 0.0 0.0 0.09623095429029671 0.0 31 0.0 0.0 0.0 0.09623095429029671 0.0 32 0.0 0.0 0.0 0.09623095429029671 0.0 33 0.0 0.0 0.0 0.09623095429029671 0.0 34 0.0 0.0 0.0 0.09623095429029671 0.0 35 0.0 0.0 0.0 0.09623095429029671 0.0 36 0.0 0.0 0.0 0.12830793905372895 0.0 37 0.0 0.0 0.0 0.1764234161988773 0.0 38 0.0 0.0 0.0 0.19246190858059342 0.0 39 0.0 0.0 0.0 0.22453889334402566 0.0 40 0.0 0.0 0.0 0.24057738572574178 0.0 41 0.0 0.0 0.0 0.2566158781074579 0.0 42 0.0 0.0 0.0 0.28869286287089013 0.0 43 0.0 0.0 0.0 0.30473135525260625 0.0 44 0.0 0.0 0.0 0.30473135525260625 0.0 45 0.0 0.0 0.0 0.3368083400160385 0.0 46 0.0 0.0 0.0 0.38492381716118684 0.0 47 0.0 0.0 0.0 0.4330392943063352 0.0 48 0.0 0.0 0.0 0.4490777866880513 0.0 49 0.0 0.0 0.0 0.4490777866880513 0.0 50 0.0 0.0 0.0 0.4971932638331997 0.0 51 0.0 0.0 0.0 0.5934242181234964 0.0 52 0.0 0.0 0.0 0.6415396952686447 0.0 53 0.0 0.0 0.0 0.6896551724137931 0.0 54 0.0 0.0 0.0 0.6896551724137931 0.0 55 0.0 0.0 0.0 0.6896551724137931 0.0 56 0.0 0.0 0.0 0.6896551724137931 0.0 57 0.0 0.0 0.0 0.7217321571772254 0.0 58 0.0 0.0 0.0 0.7217321571772254 0.0 59 0.0 0.0 0.0 0.7377706495589414 0.0 60 0.0 0.0 0.0 0.7377706495589414 0.0 61 0.0 0.0 0.0 0.7538091419406576 0.0 62 0.0 0.0 0.0 0.7538091419406576 0.0 63 0.0 0.0 0.0 0.7698476343223737 0.0 64 0.0 0.0 0.0 0.7858861267040899 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATAAA 15 0.0021226748 69.991936 47 TTTACAC 25 2.2110387E-4 55.99355 2 CTTTACA 20 0.006423329 52.92073 1 CCGACGA 45 8.192256E-5 38.88441 20 TACACAT 50 1.523682E-4 34.995968 4 TCTGACG 55 2.668069E-4 31.814516 10 CATCTGA 45 0.003991552 31.107529 8 GACGCTG 45 0.003991552 31.107529 13 GCCGACG 45 0.003991552 31.107529 19 ATCTGAC 45 0.003991552 31.107529 9 CACATCT 60 4.445041E-4 29.163307 6 TTACACA 50 0.0066831005 27.996775 3 CTGACGC 50 0.0066831005 27.996775 11 ACATCTG 50 0.0066831005 27.996775 7 TGACGCT 50 0.0066831005 27.996775 12 ACACATC 75 0.0016375037 23.330647 5 >>END_MODULE