FastQCFastQC Report
Wed 25 May 2016
SRR1780526_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780526_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6235
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT170027.265437048917402Illumina PCR Primer Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT71511.467522052927025No Hit
CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC5939.510825982357659No Hit
CTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC2163.4643143544506816No Hit
TATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCT1342.14915797914996No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG801.2830793905372895No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG560.8981555733761026No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGTCTT550.8821170809943866No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG510.8179631114675221No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGT470.7538091419406576No Hit
CTTAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC460.7377706495589414No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC460.7377706495589414No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC440.7056936647955092No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGTCTT350.5613472333600642No Hit
ACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCT330.5292702485966319Illumina PCR Primer Index 2 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGTCTT310.4971932638331997No Hit
CTTATCACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC290.46511627906976744No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTTTGTATCTCGTATGCCGT270.4330392943063352No Hit
TTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC260.4170008019246191No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGT240.38492381716118684No Hit
CTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCT230.3688853247794707No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGT230.3688853247794707No Hit
ATACACATCTCCGAGCCCACGAGACCCGCTTGTATCTCGTATGCCGTCTT220.3528468323977546TruSeq Adapter, Index 8 (95% over 22bp)
CTTATTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG220.3528468323977546No Hit
TCTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT190.30473135525260625No Hit
CTTATATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC180.28869286287089013No Hit
CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGTC170.272654370489174No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG170.272654370489174No Hit
ATACACATCTCCGAGCCCACGAGACCCGTCTGTATCTCGTATGCCGTCTT170.272654370489174Illumina PCR Primer Index 2 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC160.2566158781074579No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTCTCTCGTATGCCGTCTT160.2566158781074579No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCCT150.24057738572574178No Hit
ATACACATCTCCGAGCCCACGAGAACCGTTTGTATCTCGTATGCCGTCTT150.24057738572574178TruSeq Adapter, Index 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTCTGCCGTCTT150.24057738572574178No Hit
CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGTC140.22453889334402566No Hit
ATACACATCTCCGAGCCCACGAGACCCGATTGTATCTCGTATGCCGTCTT130.20850040096230954RNA PCR Primer, Index 35 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTAGTATGCCGT130.20850040096230954No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCAGTCTT130.20850040096230954No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGATTGTATCTCGTATGCCGT130.20850040096230954No Hit
CTTATACACATCTCCGAGCCCACGCGACCCGTTTGTATCTCGTATGCCGT120.19246190858059342No Hit
CTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT120.19246190858059342Illumina PCR Primer Index 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGATCCGTTTGTATCTCGTATGCCGTCTT110.1764234161988773TruSeq Adapter, Index 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACGTTTGTATCTCGTATGCCGTCTT100.16038492381716118TruSeq Adapter, Index 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGCCCCGTTTGTATCTCGTATGCCGTCTT100.16038492381716118Illumina PCR Primer Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCCCGTATGCCGT90.14434643143544507No Hit
CTTATACACATCTCCGAGCCCACGAGAACCGTTTGTATCTCGTATGCCGT80.12830793905372895No Hit
ATACACATCTCCGAGCACACGAGACCCGTTTGTATCTCGTATGCCGTCTT80.12830793905372895TruSeq Adapter, Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTAGCTCGTATGCCGT80.12830793905372895No Hit
CTATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGTC80.12830793905372895No Hit
CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGCC80.12830793905372895No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTCTGCCGT80.12830793905372895No Hit
CTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCTGCTTGA80.12830793905372895Illumina PCR Primer Index 2 (96% over 28bp)
ATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCTGCTT80.12830793905372895Illumina PCR Primer Index 2 (96% over 27bp)
ATACACATCTCCGCGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT80.12830793905372895Illumina PCR Primer Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGTC70.11226944667201283No Hit
CTTATACACATCTCCGAGCCCACGAGCCCCGTTTGTATCTCGTATGCCGT70.11226944667201283No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGTTTGTATCTCGTATGCCGT70.11226944667201283No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTCGTATCTCGTATGCCGTCTT70.11226944667201283RNA PCR Primer, Index 27 (95% over 22bp)
CTTATACACATCTCCGAGCACACGAGACCCGTTTGTATCTCGTATGCCGT70.11226944667201283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA453.6379788E-1270.000011
CTTTACA850.070.000011
CAACACA150.00212170570.069
CTTAATA150.00212170570.01
TAATACA150.00212170570.03
TTAATAC150.00212170570.02
TCTTATA308.664938E-870.01
TTTACAC900.066.1111152
TTACACA1000.059.4999963
CTTATAC1550.056.451611
AAAATAT200.00663197452.567
ACAACAC200.00663197452.568
TTATACA1550.051.9354822
ACTCGTA404.0855255E-543.7535
TTGTAAC404.0855255E-543.7530
AACTCGT404.0855255E-543.7534
TAACTCG404.0855255E-543.7533
GTAACTC404.0855255E-543.7532
TTTGTAA404.0855255E-543.7529
TGTAACT404.0855255E-543.7531
Produced by FastQC (version 0.11.5)