FastQCFastQC Report
Wed 25 May 2016
SRR1780525_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780525_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83174
Sequences flagged as poor quality0
Sequence length50
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG1030.12383677591555053No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACA207.821692E-443.9915731
CATCGGA207.844937E-443.9651039
ACATCGG207.844937E-443.9651038
TTTACAC1350.037.4292342
CTTTACA1250.036.9529231
TTACACA1550.032.5996553
GTAGAAC353.1846287E-431.44147937
CTATACA651.7091224E-830.4557041
TAGAACT300.005703178729.3453838
ATTCTTA300.005703178729.3453840
CGACGAT300.00572000829.32771522
AGAATGA300.00572000829.32771527
TTTCACA300.005753785429.2924443
GTTACAC300.005753785429.2924442
CTTATAC5800.027.3051131
CATCTGC609.670519E-625.6463099
TTATACA6200.025.5127722
GTGTATA450.001383970724.45448536
TGTAGAA450.001383970724.45448536
TCACATC450.001398627124.4103685
Produced by FastQC (version 0.11.5)