FastQCFastQC Report
Wed 25 May 2016
SRR1780525_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780525_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83174
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT3275639.38249933873566No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT2159125.958833289249046RNA PCR Primer, Index 20 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC66317.972443311611801No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG47515.71212157645418No Hit
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC33043.9723952196599894No Hit
TATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCT17302.07997691586313No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC10821.3008872965109288No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC10401.2503907471084714No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC6910.8307884675499555No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG6820.8199677783922862No Hit
CTTAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC5330.6408252578930916No Hit
CTTATAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG5280.6348137639166086No Hit
TTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC4800.5771034217423714No Hit
CTTATTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG4100.49294250607160894No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG3580.4304229687161853No Hit
ACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTTCT3010.3618919373842787RNA PCR Primer, Index 20 (95% over 23bp)
CTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCT2870.34505975425012625No Hit
CTTATCACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC2640.3174068819583043No Hit
ATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTTCTGCTT2540.3053838940053382RNA PCR Primer, Index 34 (96% over 28bp)
CTTATATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC1790.21521148435809265No Hit
TCTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT1770.21280688676749948No Hit
TCTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT1570.18876091086156732No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATATCGTATGCCGT1220.14668045302618607No Hit
CTCTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG1190.14307355664029625No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATATCGTATGCCGTCTT1160.13946666025440643No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGC1040.12503907471084713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT207.81437E-444.00000443
TCTGCTT207.81437E-444.00000444
CTTATCA408.1527105E-944.0000041
CTTACAC408.1527105E-944.0000041
TCTTCTG207.81437E-444.00000441
CTTCTGC207.81437E-444.00000442
CTTTTAT254.407199E-544.01
TTTTATA254.407199E-544.02
TTATAAC600.044.02
TCTTAAT502.7284841E-1144.01
CTTATAA650.043.9999961
CTTTACA6450.043.6589131
TTTACAC6500.043.323082
CCGTCTT23350.042.49250844
CTTAACA700.040.8571431
TTAACAC700.040.8571432
CTATACA3750.039.3066671
GCCGTCT25350.039.1400443
TTATCAC452.3062967E-839.111112
TATCACA404.063404E-738.5000043
Produced by FastQC (version 0.11.5)