##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780524_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20683 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.0640622733646 32.0 21.0 34.0 12.0 34.0 2 27.675627326790117 34.0 23.0 34.0 11.0 34.0 3 27.080887685538848 34.0 21.0 34.0 11.0 34.0 4 27.91727505680994 34.0 23.0 34.0 11.0 34.0 5 27.686505826040708 34.0 23.0 34.0 11.0 34.0 6 30.41942658221728 37.0 26.0 38.0 11.0 38.0 7 30.12058212058212 36.0 25.0 38.0 11.0 38.0 8 29.80089928927138 35.0 24.0 38.0 11.0 38.0 9 30.054005705168496 36.0 23.0 38.0 12.0 38.0 10-11 29.893656626214764 36.0 23.0 38.0 11.0 38.0 12-13 29.93538171445148 36.5 23.0 38.0 11.0 38.0 14-15 29.654039549388386 36.0 21.5 38.0 11.0 38.0 16-17 29.380384857129044 36.0 21.0 38.0 11.0 38.0 18-19 29.538026398491517 36.0 21.5 38.0 11.0 38.0 20-21 29.61047720350046 36.0 22.0 38.0 11.0 38.0 22-23 29.23906106464246 36.0 21.0 38.0 11.0 38.0 24-25 29.54849393221486 36.5 21.5 38.0 11.0 38.0 26-27 29.4946816225886 36.5 21.0 38.0 11.0 38.0 28-29 29.345138519557125 37.0 21.0 38.0 11.0 38.0 30-31 29.361117826234107 36.5 21.0 38.0 11.0 38.0 32-33 29.2016632016632 36.0 21.0 38.0 11.0 38.0 34-35 29.450756660058985 36.0 21.0 38.0 11.0 38.0 36-37 29.265822172798917 36.0 21.0 38.0 11.0 38.0 38-39 29.418048638978874 37.0 21.0 38.0 11.0 38.0 40-41 29.197529371947976 36.0 21.0 38.0 11.0 38.0 42-43 29.450877532272884 36.0 21.0 38.0 11.0 38.0 44-45 28.772566842334285 34.5 21.0 38.0 11.0 38.0 46-47 29.327539525213943 36.0 21.0 38.0 11.0 38.0 48-49 29.425446018469273 36.0 21.0 38.0 11.0 38.0 50-51 29.227723250979068 35.5 21.0 38.0 11.0 38.0 52-53 29.185804767200118 35.5 21.0 38.0 11.0 38.0 54-55 29.00079775661171 34.5 21.0 38.0 11.0 38.0 56-57 28.892713822946384 35.0 21.0 38.0 11.0 38.0 58-59 28.844364937388193 35.0 21.0 38.0 11.0 38.0 60-61 28.94662283034376 34.5 21.0 38.0 11.0 38.0 62-63 28.750398878305855 34.5 20.5 38.0 11.0 38.0 64-65 28.949475414591692 35.5 20.5 38.0 11.0 38.0 66-67 28.950780834501764 35.0 20.5 38.0 11.0 38.0 68-69 28.528695063578784 34.0 20.0 38.0 11.0 38.0 70-71 28.566745636513076 34.0 20.0 38.0 11.0 38.0 72-73 28.577285693564765 34.0 20.0 38.0 11.0 38.0 74-75 28.090533288207705 33.5 19.0 38.0 11.0 38.0 76 27.383551709133105 32.0 19.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.5752410770427083 1101 2 -2.0505284201990683 1101 3 -0.5903768581057847 1101 4 -0.34103214980466134 1101 5 0.20005542722408265 1101 6 0.21383095680069886 1101 7 -1.687371861072691 1101 8 0.6650945771906116 1101 9 -0.3322640357116917 1101 10-11 -1.0970873180119831 1101 12-13 -1.6511759523164855 1101 14-15 0.6220531744919207 1101 16-17 -0.5081995970338582 1101 18-19 -0.011034583022283329 1101 20-21 -0.20159374997950152 1101 22-23 -0.20423438761317314 1101 24-25 -0.5355614916112437 1101 26-27 -0.5769232516344225 1101 28-29 -1.20344982559471 1101 30-31 -2.1159689781882953 1101 32-33 0.08599571671812001 1101 34-35 -1.8075680289020113 1101 36-37 -1.1734462745062615 1101 38-39 -1.3962044566172729 1101 40-41 0.6240356523285655 1101 42-43 -0.2679829538713179 1101 44-45 -0.07890683764968287 1101 46-47 -0.0848202921745198 1101 48-49 -2.191511510044787 1101 50-51 -1.9496915051725665 1101 52-53 0.014652463643898272 1101 54-55 -1.9380023767028867 1101 56-57 -1.4979568146067237 1101 58-59 -1.036373160620176 1101 60-61 -0.6938189840026645 1101 62-63 -2.059336099147213 1101 64-65 -1.449290799603201 1101 66-67 -0.8804287850439216 1101 68-69 -0.939495432307691 1101 70-71 -0.7232715942217389 1101 72-73 -0.5841703145116313 1101 74-75 -0.37533272629140413 1101 76 -1.4647711092839444 1102 1 0.6191028143600086 1102 2 0.20263900061541307 1102 3 0.3738765355593756 1102 4 -0.06931269279108676 1102 5 -0.6513020388392654 1102 6 -0.012413387090703054 1102 7 -0.5391818158238202 1102 8 -1.9018737486012434 1102 9 0.29556401858695125 1102 10-11 -0.7665443315866476 1102 12-13 -3.175044730597026 1102 14-15 -2.4044174137433743 1102 16-17 -0.9154394160383887 1102 18-19 -0.32574046537522605 1102 20-21 -0.39808696264918453 1102 22-23 0.21205520514700993 1102 24-25 -0.7359008581270778 1102 26-27 -3.503506962041662 1102 28-29 -0.5218887396218648 1102 30-31 -0.8323716931204181 1102 32-33 -5.8956784914266755 1102 34-35 -1.2106223275445451 1102 36-37 -0.3771793061804729 1102 38-39 -0.9397564928163682 1102 40-41 -1.4053761123773185 1102 42-43 -0.8566707366767474 1102 44-45 -4.872232629504886 1102 46-47 -1.4287116948894507 1102 48-49 -0.23608164579139057 1102 50-51 -5.0599856228196245 1102 52-53 -3.360234414184159 1102 54-55 -1.2978440056621672 1102 56-57 -1.3547441449234654 1102 58-59 -1.3393143370907659 1102 60-61 -1.9993619704280015 1102 62-63 -3.4500600810476634 1102 64-65 -1.5622998493769558 1102 66-67 -1.91300797056428 1102 68-69 -5.296735251312214 1102 70-71 -1.776778381552056 1102 72-73 -0.17987167197769338 1102 74-75 -4.796147205929415 1102 76 -3.3202009735373395 1103 1 1.3190646172629883 1103 2 0.40221883254818636 1103 3 0.8028299351010091 1103 4 1.2681586593861454 1103 5 1.1666887938574497 1103 6 -1.1697854268156824 1103 7 1.0049358312350023 1103 8 2.2467129588089954 1103 9 -0.08850779195545044 1103 10-11 0.7134021924775276 1103 12-13 0.4333395321989997 1103 14-15 0.567507719946466 1103 16-17 -0.12208571091997555 1103 18-19 1.4811732091855063 1103 20-21 1.4419626935769436 1103 22-23 0.8182431348643533 1103 24-25 1.9125903565406084 1103 26-27 1.0850148103036403 1103 28-29 0.7950516729067871 1103 30-31 1.6585564963371802 1103 32-33 -2.868749538027135 1103 34-35 -0.7573182786522636 1103 36-37 0.574405873345885 1103 38-39 1.404494844082027 1103 40-41 2.0655940938870074 1103 42-43 1.5680809821926154 1103 44-45 -2.2254602842031304 1103 46-47 1.5445503371961102 1103 48-49 1.0146822961490223 1103 50-51 0.7581007026196431 1103 52-53 0.7293377783292136 1103 54-55 0.8493103106097983 1103 56-57 1.6962489795990692 1103 58-59 1.8805099562629373 1103 60-61 1.2008863107026322 1103 62-63 0.18382074400963333 1103 64-65 1.073586323273922 1103 66-67 0.6408499362348046 1103 68-69 -2.3053295981418565 1103 70-71 1.5682368972867522 1103 72-73 1.6816638513225364 1103 74-75 0.8004914495327711 1103 76 0.39656755205471583 1104 1 -1.5080124152934964 1104 2 -0.8189161968060219 1104 3 -0.10839582543981763 1104 4 -0.2870806218805342 1104 5 -0.5540014747780262 1104 6 -1.0696253129569406 1104 7 -0.2964340317786949 1104 8 2.0147257679176924 1104 9 0.7341216334137037 1104 10-11 -0.002039899676894663 1104 12-13 -0.639690177817009 1104 14-15 0.33027235257410936 1104 16-17 -0.6225304716387114 1104 18-19 -0.5644592405565305 1104 20-21 -0.10749469834620484 1104 22-23 -0.03774334440980098 1104 24-25 0.3115407212443948 1104 26-27 -0.09546908935834253 1104 28-29 -0.3140926074703714 1104 30-31 -0.05306065363613399 1104 32-33 -0.9027292569383611 1104 34-35 0.6410094210453039 1104 36-37 0.4802945052619165 1104 38-39 0.4704973347420527 1104 40-41 -0.1704848956166387 1104 42-43 1.4797515034521815 1104 44-45 -1.37374350782882 1104 46-47 -0.6970436852198283 1104 48-49 0.7069078787855609 1104 50-51 0.125650960580856 1104 52-53 -0.035294849317114796 1104 54-55 -0.12409299840994947 1104 56-57 0.5016216890813681 1104 58-59 0.5992432777359831 1104 60-61 0.16592811821019282 1104 62-63 0.8833404024334008 1104 64-65 0.21519813611861238 1104 66-67 0.03864392306988762 1104 68-69 -3.0209495945837688 1104 70-71 -0.20093228969065535 1104 72-73 1.2465989162576 1104 74-75 0.14100025579499942 1104 76 -0.7083959775663473 1105 1 -0.15003255062936205 1105 2 -0.21568133956885305 1105 3 -1.356456561534884 1105 4 0.1753719280451982 1105 5 1.1114548711536472 1105 6 1.7884370735754942 1105 7 0.5230081203916299 1105 8 1.0949370239005276 1105 9 1.729830496262359 1105 10-11 1.5217263983920937 1105 12-13 -0.9974224768762596 1105 14-15 -0.2972239339418188 1105 16-17 0.5682600878595565 1105 18-19 0.5745075856524124 1105 20-21 0.549379373282779 1105 22-23 0.1433096346296452 1105 24-25 0.3953004175639201 1105 26-27 -0.035310647371215254 1105 28-29 -0.9856555716558795 1105 30-31 -0.4809365407462174 1105 32-33 0.0315008140304478 1105 34-35 -0.08402771379543239 1105 36-37 0.35519135292654624 1105 38-39 1.3475397509823566 1105 40-41 1.1300597487141104 1105 42-43 0.6635277041453627 1105 44-45 1.196069992748832 1105 46-47 -0.17286477780936593 1105 48-49 -0.18771169540159605 1105 50-51 0.19488680808044734 1105 52-53 -0.5751529117036469 1105 54-55 -0.8755371311051121 1105 56-57 0.32048618817362495 1105 58-59 0.5286870803436763 1105 60-61 0.7560419983884366 1105 62-63 -0.1547021788506413 1105 64-65 0.04338760632821703 1105 66-67 0.4533988331210459 1105 68-69 0.9309401562001902 1105 70-71 -0.10075074158040564 1105 72-73 -0.036533613862882675 1105 74-75 -0.7029508912589684 1105 76 -0.6697757432042408 1106 1 -0.7137026155042463 1106 2 -1.0043745740452223 1106 3 -0.9749922427211679 1106 4 -1.8794936882662014 1106 5 -1.1384061112374582 1106 6 -0.7861690431993011 1106 7 -1.1796795533803817 1106 8 -0.781059268963233 1106 9 -1.1784178818655384 1106 10-11 -1.2740103949350647 1106 12-13 -1.4819451830857133 1106 14-15 -1.3856391332003888 1106 16-17 -1.8312765201107837 1106 18-19 -1.5725730445607446 1106 20-21 -0.08620913459488477 1106 22-23 -1.3350036183824017 1106 24-25 -2.1278691839189356 1106 26-27 -0.7000001747113451 1106 28-29 -1.557295979440866 1106 30-31 -1.1159689781882953 1106 32-33 -1.3909273602049552 1106 34-35 -0.4229526442866245 1106 36-37 -1.3503693514293396 1106 38-39 -1.5731275335403474 1106 40-41 -1.6528874245945104 1106 42-43 0.3474016615132989 1106 44-45 -0.8635222222650683 1106 46-47 -1.0078972152514396 1106 48-49 -0.699203817737093 1106 50-51 -2.2266145820956424 1106 52-53 -2.000732151740717 1106 54-55 -0.9995408382413515 1106 56-57 -0.9056491222990317 1106 58-59 -0.5056039298509489 1106 60-61 -1.2399728301565105 1106 62-63 -0.7285668683779782 1106 64-65 0.35840150808910565 1106 66-67 -1.334274938890072 1106 68-69 -0.5087262015384617 1106 70-71 -1.123271594221741 1106 72-73 -1.6764780068193232 1106 74-75 -1.6445634955221777 1106 76 -1.495540340053175 1107 1 0.4449106022639029 1107 2 -0.4951654732868249 1107 3 0.003122600182408064 1107 4 -0.4121047391871109 1107 5 0.3861876049055546 1107 6 -0.13503144839973302 1107 7 -0.009148675807249873 1107 8 0.3818876432794518 1107 9 -1.0733257908579539 1107 10-11 -0.37238525950710866 1107 12-13 -0.4856288233890709 1107 14-15 -0.9004820367756885 1107 16-17 -0.5910815038594279 1107 18-19 -0.1208937379518602 1107 20-21 -0.23994694607917566 1107 22-23 -0.942045871903531 1107 24-25 -0.15874675705002872 1107 26-27 -0.4644638800201193 1107 28-29 -1.031727182041081 1107 30-31 -0.2628812208751832 1107 32-33 1.2760282194266814 1107 34-35 -0.4458128826398209 1107 36-37 -1.810174335177983 1107 38-39 0.5133296712267175 1107 40-41 -0.5872319533052384 1107 42-43 0.32269960300842015 1107 44-45 0.6690888286558447 1107 46-47 0.11561307727293624 1107 48-49 -0.7346317700664535 1107 50-51 -0.15391685728524251 1107 52-53 0.7754325286493149 1107 54-55 0.619202390361032 1107 56-57 -0.1320846585937261 1107 58-59 -0.3560914704793312 1107 60-61 -0.8776759720850045 1107 62-63 0.10772782284628946 1107 64-65 0.41170595012594546 1107 66-67 0.3811096764945425 1107 68-69 -0.37730691659804805 1107 70-71 -1.2060451586854413 1107 72-73 -0.999879956981836 1107 74-75 -1.0405548281332244 1107 76 0.19243365777997568 1108 1 -1.4987513695670849 1108 2 -0.19939082539950093 1108 3 1.594671895957056 1108 4 0.3907121622213374 1108 5 0.8368918302576667 1108 6 0.17482770652984314 1108 7 0.7654992115166941 1108 8 0.45231017849071975 1108 9 0.09568829364908638 1108 10-11 0.26845981091542725 1108 12-13 0.8523993456250096 1108 14-15 -0.06245386776160089 1108 16-17 -1.6615040390706994 1108 18-19 -0.3884993717546763 1108 20-21 -0.08501736861438758 1108 22-23 0.4382358182373167 1108 24-25 0.5877321161894109 1108 26-27 0.5003248523742485 1108 28-29 0.36263901514201535 1108 30-31 0.09627370870228091 1108 32-33 0.6915211771731613 1108 34-35 -0.4458128826398209 1108 36-37 -0.6200334901075628 1108 38-39 0.0696676993957297 1108 40-41 0.31417649739898934 1108 42-43 0.46354467343095607 1108 44-45 -0.23231962204838297 1108 46-47 0.6649088519208206 1108 48-49 0.5259316102152347 1108 50-51 -1.2102548854542547 1108 52-53 -0.4217505699422368 1108 54-55 -0.04276944062488397 1108 56-57 -0.18138043324161046 1108 58-59 -0.9898942873807393 1108 60-61 0.05894374622485543 1108 62-63 0.22040387918431392 1108 64-65 1.0736777811118579 1108 66-67 0.768433620156511 1108 68-69 0.7494536467822357 1108 70-71 1.0333914610328634 1108 72-73 1.021246803581544 1108 74-75 0.6988817915850838 1108 76 1.2910252070757515 1109 1 -2.003446205247837 1109 2 -0.52418809385874 1109 3 -1.56729993502886 1109 4 -2.000239609012123 1109 5 -1.4307138035451494 1109 6 -0.23255598958624546 1109 7 0.7322085585077289 1109 8 1.0713882834843176 1109 9 -0.37422207766973514 1109 10-11 -0.006294777219441272 1109 12-13 -0.30583795697848615 1109 14-15 -0.2961286436899009 1109 16-17 -0.004120342954607281 1109 18-19 -1.1973982193859172 1109 20-21 -0.5948338432195932 1109 22-23 0.4816630482842612 1109 24-25 0.19614014009038883 1109 26-27 0.3296035548923868 1109 28-29 0.6240560019111143 1109 30-31 0.6758725136532 1109 32-33 0.28168336240576863 1109 34-35 0.2610799897460083 1109 36-37 0.16315046209047424 1109 38-39 -0.5348990953119106 1109 40-41 -0.9138431355502235 1109 42-43 0.09297275708439301 1109 44-45 -0.818533877276721 1109 46-47 -0.15999511734934302 1109 48-49 -0.31540428393755704 1109 50-51 -0.3512066566877152 1109 52-53 0.41115596014739353 1109 54-55 -0.7232005119010232 1109 56-57 0.16486369821378588 1109 58-59 -0.8272822515292688 1109 60-61 0.8275096873260068 1109 62-63 -0.17332211313322432 1109 64-65 -0.594162594474998 1109 66-67 -0.4370721416872776 1109 68-69 0.9933717005594431 1109 70-71 -1.0345769555271005 1109 72-73 -0.4124919928333064 1109 74-75 1.4574178564591804 1109 76 0.7212428767300416 1110 1 0.21160755819302324 1110 2 0.913739569875375 1110 3 1.1907645811001721 1110 4 1.5061142521804491 1110 5 0.6338023254871104 1110 6 2.1716473339719187 1110 7 0.9242906699446785 1110 8 0.6256404828978077 1110 9 0.5275374531220542 1110 10-11 0.7742526323602235 1110 12-13 0.3266156109589531 1110 14-15 0.8123757551370794 1110 16-17 0.059418268971100474 1110 18-19 1.0115460621390042 1110 20-21 0.7155278381098285 1110 22-23 2.097254446133725 1110 24-25 1.1196494265028996 1110 26-27 0.8667492049412608 1110 28-29 1.7829025317253873 1110 30-31 1.7987952898017845 1110 32-33 2.1223480740382215 1110 34-35 0.4248141050930272 1110 36-37 0.8645189860396414 1110 38-39 1.4660784217946379 1110 40-41 0.8175840394253413 1110 42-43 1.901868162753992 1110 44-45 2.581514998578605 1110 46-47 0.9778347946741164 1110 48-49 1.5589847678708502 1110 50-51 1.3083730109564655 1110 52-53 0.7322703296488591 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-1.0004123905960718 2116 62-63 -0.21098445079556072 2116 64-65 0.9605993102869093 2116 66-67 1.1382525336374023 2116 68-69 1.315449622637363 2116 70-71 -0.9979968689470127 2116 72-73 0.33231320197188907 2116 74-75 1.343348592389912 2116 76 0.9550091104962739 2117 1 0.5770135648671086 2117 2 -0.41551515759959656 2117 3 -0.2397137281323083 2117 4 -1.0556210092211096 2117 5 -1.0514034587175622 2117 6 -1.0752937116343126 2117 7 -1.2019607204891365 2117 8 -0.10572770397649478 2117 9 -1.3083913566666006 2117 10-11 -1.3806416946698086 2117 12-13 0.05492484343948689 2117 14-15 0.10826007104364166 2117 16-17 0.39896220667966986 2117 18-19 -0.259310445091252 2117 20-21 -0.5175088693428975 2117 22-23 0.16844465747966808 2117 24-25 -0.9501503510276876 2117 26-27 -0.19442988293415908 2117 28-29 -0.32944452055492235 2117 30-31 -0.13029258561534718 2117 32-33 -1.5158610472076077 2117 34-35 -0.44921259123622903 2117 36-37 -0.8718547625699244 2117 38-39 -1.6879816449461877 2117 40-41 -1.289757451119712 2117 42-43 0.7447491416193976 2117 44-45 0.3126050986898363 2117 46-47 0.2812274531835719 2117 48-49 -2.282227690416139 2117 50-51 -0.1565880568967053 2117 52-53 -1.0230133188494683 2117 54-55 -0.6231482652970541 2117 56-57 1.7137142729264312 2117 58-59 1.832592356621202 2117 60-61 1.248356082310334 2117 62-63 2.6244835295000044 2117 64-65 0.9546879802376473 2117 66-67 1.7776614006324714 2117 68-69 1.3021491300265282 2117 70-71 2.2822986975554507 2117 72-73 0.8845299507403581 2117 74-75 1.103447114557401 2117 76 1.0535312779839607 2118 1 0.06322046141882964 2118 2 -0.9454002150708618 2118 3 -0.3672999350288606 2118 4 -3.582057790830305 2118 5 1.0692861964548506 2118 6 -4.081040838071093 2118 7 -1.9950641687649977 2118 8 -2.7528541407581066 2118 9 0.16517186172420395 2118 10-11 0.111887040962376 2118 12-13 -1.092201593342125 2118 14-15 -2.2779468255080815 2118 16-17 -1.7889688278030924 2118 18-19 -2.394367916355616 2118 20-21 -0.2721065704923227 2118 22-23 -1.9850036183824002 2118 24-25 -0.4432537993035517 2118 26-27 -1.4294873541985247 2118 28-29 0.2619347897899047 2118 30-31 -1.771097183316499 2118 32-33 -2.2486196678972625 2118 34-35 -0.5267987981327806 2118 36-37 -3.208061659121647 2118 38-39 -2.433383943796759 2118 40-41 -1.6259643476714345 2118 42-43 -1.4282393641277302 2118 44-45 -1.0942914530343018 2118 46-47 -0.9463587537129783 2118 48-49 -1.4851012536345287 2118 50-51 -1.9496915051725665 2118 52-53 -4.049450100458667 2118 54-55 -2.144412633113145 2118 56-57 -1.3851363017862113 2118 58-59 -0.8197064939535146 2118 60-61 -2.636126676310358 2118 62-63 -2.365746355557466 2118 64-65 -2.8774959278083294 2118 66-67 -2.835556990172126 2118 68-69 -1.8035979964102538 2118 70-71 -1.886092107042252 2118 72-73 -1.5700677504090663 2118 74-75 -2.3971275980862785 2118 76 -2.621181365694202 2119 1 1.7822680804664515 2119 2 0.783171213500566 2119 3 1.3612714935425672 2119 4 1.9607993520268394 2119 5 -0.002142374973722383 2119 6 0.9903877333574762 2119 7 1.0049358312350023 2119 8 -0.42428271218667746 2119 9 2.3746956712480127 2119 10-11 0.7523632314385651 2119 12-13 0.3316079304673991 2119 14-15 2.4268150792538243 2119 16-17 2.3086502198159558 2119 18-19 1.6651558931681905 2119 20-21 0.5826553342695853 2119 22-23 2.6126154292366444 2119 24-25 1.5900795340297869 2119 26-27 3.4919412172300497 2119 28-29 1.5071728850279982 2119 30-31 2.8836647214454096 2119 32-33 2.358523189245595 2119 34-35 2.247010725676745 2119 36-37 2.875271674211689 2119 38-39 3.449949389536574 2119 40-41 3.3621308904238063 2119 42-43 1.3646177787294143 2119 44-45 3.4628514041085587 2119 46-47 3.191736484382261 2119 48-49 3.607755889222613 2119 50-51 2.002689447208386 2119 52-53 1.8267403757318093 2119 54-55 2.5008254621249506 2119 56-57 2.867244650594742 2119 58-59 4.21600779176077 2119 60-61 2.2138733236896435 2119 62-63 1.131872692061581 2119 64-65 2.8463135960011954 2119 66-67 1.1430144383993053 2119 68-69 1.2249734321611747 2119 70-71 1.6591459881958457 2119 72-73 1.0085036781623629 2119 74-75 0.6814438304851507 2119 76 0.859771015258179 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 15.0 13 89.0 14 322.0 15 623.0 16 923.0 17 1056.0 18 1151.0 19 1022.0 20 859.0 21 596.0 22 436.0 23 311.0 24 254.0 25 218.0 26 194.0 27 212.0 28 213.0 29 259.0 30 295.0 31 394.0 32 527.0 33 745.0 34 1088.0 35 1741.0 36 3146.0 37 3994.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.476885644768856 22.38442822384428 9.289537712895376 39.84914841849149 2 14.586858772905284 32.828893294009575 27.616883430836918 24.967364502248223 3 19.15582845815404 26.949668810133925 26.519363728666058 27.37513900304598 4 9.901851762316879 31.44128027848958 28.960982449354546 29.695885509838998 5 9.476381569404825 36.121452400522166 34.60813228255088 19.794033747522118 6 19.27186578349369 34.82570226756273 15.984141565536913 29.918290383406664 7 18.788376927911813 31.49446405260359 17.178359038824155 32.53879998066044 8 15.945462457090365 40.598559203210364 17.15901948460088 26.29695885509839 9 18.07764831020645 24.604747860561815 17.555480346178022 39.76212348305371 10-11 17.27747425421844 28.039936179471063 25.417009137939374 29.265580428371123 12-13 17.988202871923804 25.895663104965433 24.50079775661171 31.615336266499057 14-15 18.783542039355993 27.761930087511484 21.50558429628197 31.948943576850553 16-17 20.26301793743654 28.745829908620607 22.750568099405307 28.240584054537543 18-19 18.60223371851279 27.98433496107915 22.576512111395832 30.83691920901223 20-21 16.96804138664604 28.194652613257265 23.915776241357637 30.921529758739062 22-23 21.38712952666441 27.522603103998456 22.5426678915051 28.547599477832037 24-25 19.51844509984045 30.38002224048736 22.213895469709424 27.88763718996277 26-27 17.39351157955809 31.58874437944205 24.000386791084466 27.017357249915392 28-29 19.803703524633757 28.39046559976793 26.226852971039015 25.578977904559302 30-31 19.26703089493787 27.715998646231206 27.244597012038874 25.772373446792052 32-33 16.96804138664604 31.3179906203162 25.436348692162646 26.277619300875116 34-35 18.099405308707635 29.338103756708406 25.134168157423968 27.428322777159984 36-37 18.55146738867669 28.284098051539914 26.12773775564473 27.036696804138664 38-39 18.09215297587391 26.519363728666058 28.315524827152732 27.072958468307306 40-41 17.07199149059614 26.427500846105495 27.039114248416574 29.461393414881787 42-43 15.503070154232946 25.595900014504664 28.953730116520816 29.947299714741575 44-45 15.539331818401585 26.848136150461734 29.5677609631098 28.04477106802688 46-47 16.225885993327854 27.9988396267466 27.396896001547166 28.37837837837838 48-49 15.665038920852876 29.398539863656143 25.842479330851425 29.093941884639555 50-51 15.377363051781657 28.975487115022 25.49436735483247 30.152782478363875 52-53 15.217811729439637 27.60721365372528 26.393656626214767 30.781317990620316 54-55 15.147705845380264 28.433979596770293 25.155925155925157 31.262389401924285 56-57 16.7528888459121 28.17531305903399 24.47420586955471 30.597592225499206 58-59 16.39752453705942 26.736933713677903 25.054392496252966 31.811149253009717 60-61 15.428129381617753 26.65715805250689 25.286467146932264 32.628245418943095 62-63 16.332253541555865 27.65556253928347 24.312237102934777 31.69994681622589 64-65 16.68036551757482 28.078615287917614 22.90044964463569 32.34056954987188 66-67 16.465212976840885 29.553256297442342 21.79326016535319 32.188270560363584 68-69 15.88019146158681 29.879611274960112 21.80292994246483 32.43726732098825 70-71 17.64009089590485 29.417879417879416 21.720736837015906 31.221292849199827 72-73 17.207368370159067 29.176134990088475 20.920562781027897 32.69593385872456 74-75 17.944688874921432 28.124546729197892 21.377459749552774 32.5533046463279 76 18.188850746990283 25.38316491804864 21.679640284291445 34.74834405066963 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.0 10 1.0 11 0.5 12 0.0 13 0.0 14 0.5 15 1.5 16 1.5 17 1.0 18 5.0 19 8.0 20 7.0 21 7.0 22 11.0 23 17.0 24 18.0 25 17.0 26 31.5 27 49.5 28 70.5 29 88.0 30 101.5 31 125.0 32 169.0 33 203.0 34 256.0 35 354.0 36 465.0 37 531.0 38 584.0 39 766.5 40 957.0 41 1108.0 42 1198.0 43 1244.5 44 1358.0 45 1455.0 46 1485.0 47 1538.0 48 1521.5 49 1412.0 50 1372.0 51 1271.0 52 1119.5 53 1005.5 54 942.0 55 859.0 56 686.5 57 549.0 58 501.0 59 413.5 60 291.0 61 219.5 62 183.0 63 166.5 64 117.5 65 76.5 66 63.0 67 58.0 68 48.5 69 37.5 70 26.5 71 17.0 72 15.5 73 13.5 74 10.5 75 8.0 76 5.0 77 5.5 78 6.0 79 3.0 80 4.0 81 4.5 82 5.0 83 6.0 84 4.0 85 1.5 86 0.5 87 0.0 88 0.5 89 1.5 90 1.0 91 0.5 92 1.0 93 1.5 94 2.0 95 2.0 96 2.0 97 2.0 98 1.0 99 2.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6430401779238989 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 20683.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.17139679930378 #Duplication Level Percentage of deduplicated Percentage of total 1 88.89078110808356 75.7095198955664 2 7.283151680290645 12.406324034231012 3 2.4693460490463215 6.309529565343519 4 0.7720254314259765 2.630179374365421 5 0.2838328792007266 1.2087221389546972 6 0.16462306993642145 0.8412706087124692 7 0.07947320617620346 0.47381907847024124 8 0.03405994550408719 0.23207465067930183 9 0.011353315168029064 0.0870279940047382 >10 0.011353315168029064 0.10153265967219455 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.004834888555818788 0.0 6 0.0 0.0 0.0 0.004834888555818788 0.0 7 0.0 0.0 0.0 0.004834888555818788 0.0 8 0.0 0.0 0.0 0.004834888555818788 0.0 9 0.0 0.0 0.0 0.004834888555818788 0.0 10 0.0 0.0 0.0 0.004834888555818788 0.0 11 0.0 0.0 0.0 0.004834888555818788 0.0 12 0.0 0.0 0.0 0.004834888555818788 0.0 13 0.0 0.0 0.0 0.004834888555818788 0.0 14 0.0 0.0 0.0 0.004834888555818788 0.0 15 0.0 0.0 0.0 0.004834888555818788 0.0 16 0.0 0.0 0.0 0.004834888555818788 0.0 17 0.0 0.0 0.0 0.009669777111637577 0.0 18 0.0 0.0 0.0 0.009669777111637577 0.0 19 0.0 0.0 0.0 0.019339554223275154 0.0 20 0.0 0.0 0.0 0.019339554223275154 0.0 21 0.0 0.0 0.0 0.019339554223275154 0.0 22 0.0 0.0 0.0 0.024174442779093943 0.0 23 0.0 0.0 0.0 0.02900933133491273 0.0 24 0.0 0.0 0.0 0.02900933133491273 0.0 25 0.0 0.0 0.0 0.02900933133491273 0.0 26 0.0 0.0 0.0 0.048348885558187886 0.0 27 0.0 0.0 0.0 0.05801866266982546 0.0 28 0.0 0.0 0.0 0.07735821689310061 0.0 29 0.0 0.0 0.0 0.09669777111637577 0.0 30 0.0 0.0 0.0 0.10153265967219456 0.0 31 0.0 0.0 0.0 0.14021176811874486 0.0 32 0.0 0.0 0.0 0.1692210994536576 0.0 33 0.0 0.0 0.0 0.18856065367693275 0.0 34 0.0 0.0 0.0 0.23690953923512062 0.0 35 0.0 0.0 0.0 0.30459797901658364 0.0 36 0.0 0.0 0.0 0.4206353043562346 0.0 37 0.0 0.0 0.0 0.5946912923657109 0.0 38 0.0 0.0 0.0 0.8944543828264758 0.0 39 0.0 0.0 0.0 1.2038872503988782 0.0 40 0.0 0.0 0.0 1.5665038920852874 0.0 41 0.0 0.0 0.0 2.108011410336992 0.0 42 0.0 0.0 0.0 2.7027027027027026 0.0 43 0.0 0.0 0.0 3.3070637721800513 0.0 44 0.0 0.0 0.0 3.8775806217666684 0.0 45 0.0 0.0 0.0 4.4045834743509165 0.0 46 0.0 0.0 0.0 4.90741188415607 0.0 47 0.0 0.0 0.0 5.328047188512305 0.0 48 0.0 0.0 0.0 5.763187158535996 0.0 49 0.0 0.0 0.0 6.237006237006237 0.0 50 0.0 0.0 0.0 6.691485761253203 0.0 51 0.0 0.0 0.0 7.22332350239327 0.0 52 0.0 0.0 0.0 7.8711985688729875 0.0 53 0.0 0.0 0.0 8.57709229802253 0.0 54 0.0 0.0 0.0 9.360344244065175 0.0 55 0.0 0.0 0.0 9.984044867765798 0.0 56 0.0 0.0 0.0 10.49654305468259 0.0 57 0.0 0.0 0.0 10.994536575931924 0.0 58 0.0 0.0 0.0 11.468355654402165 0.0 59 0.0 0.0 0.0 11.884156070202582 0.0 60 0.0 0.0 0.0 12.324130928782091 0.0 61 0.0 0.0 0.0 12.831794227143064 0.0 62 0.0 0.0 0.0 13.28143886283421 0.0 63 0.0 0.0 0.0 13.740753275636997 0.0 64 0.0 0.0 0.0 14.374123676449258 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCCA 15 0.0021940079 70.00001 2 ACCACGC 20 0.006856232 52.500004 58 TCAAATC 20 0.006856232 52.500004 5 TGTAGAT 20 0.006856232 52.500004 37 TAGATCT 20 0.006856232 52.500004 39 ACGAGCA 55 2.893578E-4 31.818184 70 >>END_MODULE