FastQCFastQC Report
Wed 25 May 2016
SRR1780524_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780524_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20683
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT18598.988057825267127TruSeq Adapter, Index 9 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT9294.4916114683556545No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC6873.321568437847507RNA PCR Primer, Index 9 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC2311.1168592563941402TruSeq Adapter, Index 9 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCT1600.7735821689310062RNA PCR Primer, Index 9 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG920.4448097471353285No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT850.41096552724459706No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGTCTT760.36745153024222793No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC720.3481119760189528No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG650.3142677561282213No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGTCTT590.2852584247933085No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGTCTT570.2755886476816709No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC540.26108398201421457TruSeq Adapter, Index 9 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC540.26108398201421457No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGT510.2465793163467582No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC500.2417444277909394No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG340.1643862108978388No Hit
CTTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCT310.14988154523038244TruSeq Adapter, Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGT300.14504665667456365No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG300.14504665667456365No Hit
TTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC300.14504665667456365TruSeq Adapter, Index 9 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTGGCTCTGATCTCGTATGCCGTCTT270.13054199100710728Illumina PCR Primer Index 9 (95% over 21bp)
CTTATCACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC260.1257071024512885TruSeq Adapter, Index 9 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG260.1257071024512885No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGTC230.11120243678383213No Hit
CTTATATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC230.11120243678383213No Hit
CTTATACACATCTCCGAGCCCACGCGACTGGATCTGATCTCGTATGCCGT210.10153265967219455No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATATA150.002194007970.0000165
CTGGACC150.002194007970.0000125
TGGACCT150.002194007970.0000126
GACCTGA150.002194007970.0000128
TTTACAC1100.066.8181842
CTTTACA1150.063.913041
TTACACA1300.056.5384643
GGACCTG252.336287E-456.00000427
CTTTATA407.1296563E-752.5000042
CCAAAAA200.00685623252.50000470
GAAGTAG200.00685623252.50000456
CTTAACA200.00685623252.5000041
TAATACA200.00685623252.5000043
AATACAC200.00685623252.5000044
TCTTTAT407.1296563E-752.5000041
CTATACA404.4392276E-543.7500041
TTTATAC601.1598344E-535.0000043
TGAAAAA7000.030.056
GATCTCG6200.029.35483733
CTTGAAA7200.029.16666854
Produced by FastQC (version 0.11.5)