FastQCFastQC Report
Wed 25 May 2016
SRR1780523_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780523_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489815
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAT250.002348587435.2086735
GCCTTGA250.002356847735.1834871
CGACCTA300.005700487629.37660817
CGCTATT300.005777926629.2956248
GTTCGTC801.3124372E-724.7383881
TCAGACT450.001383423924.48552118
CGGCTCT450.001407535924.413026
ATCGGAA752.0556781E-623.47244837
CTCGGCG752.072191E-623.45086741
CAATGGA803.5529101E-622.03922720
CCTGGTA602.866597E-422.0031722
CTATACA1900.021.9896771
CGGTGGT1800.021.98069643
TTATCGG755.4537435E-520.53839335
TATCGGA755.4537435E-520.53839336
AACATTG755.492516E-520.51950842
GGCGCTA550.004436533420.03360718
CGTGTAT550.00447347820.00492735
AGCCACG1101.2666715E-719.99266227
CGGTAAG550.00448938919.99266228
Produced by FastQC (version 0.11.5)