FastQCFastQC Report
Wed 25 May 2016
SRR1780523_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780523_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489815
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT386687.894409113644947No Hit
ATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTT313826.406908730847361No Hit
CTTTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC87521.7867970560313589No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG53601.0942907015914172No Hit
CTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC46910.9577085226054735No Hit
TATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCT32700.6675989914559578No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC31460.6422833110460072No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC19810.40443841042026074No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG12890.26316058103569717No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC10710.21865398160529995No Hit
CTTATAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG8200.16741014464644816No Hit
CTTAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC7900.16128538325694394No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC6600.1347447505690924No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG6210.12678256076273695No Hit
CTTATTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG5390.11004154629809214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGT207.8394066E-444.014410
CCGGTCG207.851245E-444.000929
GGTCGTC207.8551937E-443.99642642
TAGGTCG207.8551937E-443.99642640
CCGTCTT33650.043.08119644
GCCGTCT37850.038.3007243
CTTATAA950.037.098861
TTTACAC12100.036.724292
CTTTACA12500.036.1250151
GATCATA250.002337088635.243921
CCAGTTG250.002346481735.2151214
GTTACTC250.002352367935.19714418
CGTTACT250.002352367935.19714417
GTCGTCT250.002352367935.19714443
ATGCTCC250.002353546435.193555
CGGGCCT250.002353546435.1935536
CGCCCTA250.002353546435.1935539
TTACACA13300.033.576223
CTATACA6650.033.4552231
TCTTTAT2650.033.248981
Produced by FastQC (version 0.11.5)