FastQCFastQC Report
Wed 25 May 2016
SRR1780522_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780522_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24253
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC308.1378275E-658.3316235
TGGTTAT252.3448499E-455.99835216
GGTTATT200.006871465652.4984517
ATCGTGT200.006871465652.4984530
TTTACAA200.006871465652.4984534
ATGGTTA305.7665096E-446.66529515
CGTGTAG305.7665096E-446.66529532
GTGTAGA450.004227216431.11019733
CGACGAT950.00701519918.4205170
CCGACGA1450.004565793614.48233218
ACGCTGC2250.0063881110.88856812
Produced by FastQC (version 0.11.5)