##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780522_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24253 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.620912876757515 32.0 21.0 34.0 12.0 34.0 2 28.01529707665031 34.0 23.0 34.0 11.0 34.0 3 27.983672123036325 34.0 23.0 34.0 11.0 34.0 4 28.650888549870118 34.0 23.0 34.0 11.0 34.0 5 28.571393229703542 34.0 23.0 34.0 11.0 34.0 6 30.460437883973118 37.0 26.0 38.0 11.0 38.0 7 30.00775161835649 35.0 24.0 38.0 11.0 38.0 8 29.13837463406589 34.0 23.0 37.0 11.0 38.0 9 29.752278068692533 34.0 23.0 37.0 12.0 38.0 10-11 30.13911681029151 36.0 24.5 38.0 11.0 38.0 12-13 30.477693481218818 36.5 26.0 38.0 11.0 38.0 14-15 30.04246897291057 36.0 23.5 38.0 11.0 38.0 16-17 29.871294272873456 36.0 23.0 38.0 11.0 38.0 18-19 29.888735414175564 36.0 23.0 38.0 11.0 38.0 20-21 29.996557126953366 36.0 23.5 38.0 11.0 38.0 22-23 29.856450748361027 36.0 23.0 38.0 11.0 38.0 24-25 30.38514822908506 36.5 24.5 38.0 11.0 38.0 26-27 30.291366016575267 36.5 24.0 38.0 11.0 38.0 28-29 30.303673772316827 37.0 24.0 38.0 11.0 38.0 30-31 30.108605121015955 36.0 23.5 38.0 11.0 38.0 32-33 30.074671174700036 36.0 23.5 38.0 11.0 38.0 34-35 30.02308992701934 36.0 23.5 38.0 11.0 38.0 36-37 29.867521543726546 36.0 23.0 38.0 11.0 38.0 38-39 30.274790747536386 37.0 24.0 38.0 11.0 38.0 40-41 29.861522285902776 36.0 23.0 38.0 11.0 38.0 42-43 30.21572588958067 36.0 24.5 38.0 11.0 38.0 44-45 29.3773141467035 34.5 22.0 38.0 11.0 38.0 46-47 29.985630643631715 36.0 24.0 38.0 11.0 38.0 48-49 29.77241990681565 36.0 23.0 38.0 11.0 38.0 50-51 29.86088318970849 35.5 23.5 38.0 11.0 38.0 52-53 29.7763575640127 36.0 23.0 38.0 11.0 38.0 54-55 29.481342514328126 34.5 23.0 38.0 11.0 38.0 56-57 29.42955510658475 35.0 22.5 38.0 11.0 38.0 58-59 29.2912217045314 35.0 22.0 37.5 11.0 38.0 60-61 29.450418504927228 35.0 23.0 38.0 11.0 38.0 62-63 29.39036407867068 35.0 22.5 38.0 11.0 38.0 64-65 29.481569290397065 35.5 23.0 38.0 11.0 38.0 66-67 29.489341524759823 35.5 23.0 38.0 11.0 38.0 68-69 28.929946810703832 34.0 21.5 38.0 11.0 38.0 70-71 28.77417226734837 34.0 21.0 37.5 11.0 38.0 72-73 28.725044324413474 34.0 21.0 37.5 11.0 38.0 74-75 28.505215849585618 34.0 21.0 37.0 11.0 38.0 76 27.76670927307962 32.0 19.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.090251996062836 1101 2 -1.083598439429462 1101 3 -1.444140437599664 1101 4 -3.9827319558935628 1101 5 -2.130404573795815 1101 6 -3.750975118592148 1101 7 -2.370674774431503 1101 8 -2.3394853712878145 1101 9 -2.2029408796980263 1101 10-11 -2.8005071801569947 1101 12-13 -3.1087149852591835 1101 14-15 -3.0729868666531566 1101 16-17 -2.9577880072688316 1101 18-19 -1.4902447974691846 1101 20-21 -2.53955162244678 1101 22-23 -1.2163242234978533 1101 24-25 -2.2969641750182994 1101 26-27 -3.87247630022949 1101 28-29 -3.5509415704337997 1101 30-31 -3.8103657536504514 1101 32-33 -2.0866744753963893 1101 34-35 -2.8934238117360316 1101 36-37 -1.8952236065994157 1101 38-39 -1.8775234516820056 1101 40-41 -2.0681307973956287 1101 42-43 -1.8766463434338334 1101 44-45 -2.8416849841448304 1101 46-47 -2.1552393642295122 1101 48-49 -1.863129874384935 1101 50-51 -3.9614023223729333 1101 52-53 -2.9693405979447185 1101 54-55 -3.9311995463736196 1101 56-57 -3.004857939726758 1101 58-59 -2.8929972291343233 1101 60-61 -2.5090131105975715 1101 62-63 -3.754450860169694 1101 64-65 -2.414414482174493 1101 66-67 -3.3647120399766166 1101 68-69 -2.926459536221362 1101 70-71 -2.5949086772765995 1101 72-73 -3.3629936243789906 1101 74-75 -4.760803405246719 1101 76 -2.236953301993939 1102 1 0.437262235436215 1102 2 -0.12202348686778919 1102 3 -0.37867554196398956 1102 4 -0.7740033031421412 1102 5 -0.2893229798679222 1102 6 0.776539112906903 1102 7 -0.42760077063643465 1102 8 0.9574785755812556 1102 9 -0.1161287354855034 1102 10-11 -1.144435073895135 1102 12-13 -4.332386712014404 1102 14-15 -1.7847990108656795 1102 16-17 -2.803139050912094 1102 18-19 -1.4337931845659604 1102 20-21 -1.6111835010046534 1102 22-23 -2.4547018326060126 1102 24-25 -0.6790799245439167 1102 26-27 -4.5717172869467575 1102 28-29 -0.6128485913066655 1102 30-31 -1.0003562659844185 1102 32-33 -6.364663090197151 1102 34-35 -1.0592207756829026 1102 36-37 0.46293578618995923 1102 38-39 0.5600951441434212 1102 40-41 0.3806358060199315 1102 42-43 -0.6337620929594472 1102 44-45 -4.448895610330787 1102 46-47 -1.0155334818765702 1102 48-49 0.7122970705865974 1102 50-51 -5.137398527306523 1102 52-53 -0.9003178275462353 1102 54-55 -0.24547848375502213 1102 56-57 -1.1701805203719182 1102 58-59 0.557429715837209 1102 60-61 0.3728654472771922 1102 62-63 -4.796433782370833 1102 64-65 -0.22015452012515624 1102 66-67 -0.16475947830678805 1102 68-69 -6.442588568479426 1102 70-71 -1.294149663993867 1102 72-73 -0.9369974194454009 1102 74-75 -5.557055777163228 1102 76 -1.9874276852956463 1103 1 -1.3448853562562277 1103 2 -1.7139368761740208 1103 3 -0.5110219847390667 1103 4 -1.004891504644572 1103 5 -1.3900741950689834 1103 6 -1.2179372459088285 1103 7 -0.453672357026182 1103 8 -0.7871082560581613 1103 9 -1.5041092922685344 1103 10-11 -0.5285490818491816 1103 12-13 -1.8704998361858571 1103 14-15 -1.3625718787401802 1103 16-17 -2.219069232087527 1103 18-19 -1.3018886330856212 1103 20-21 -1.83930988191495 1103 22-23 -1.5202122170514372 1103 24-25 -1.249119694760445 1103 26-27 -1.0568034557492325 1103 28-29 -0.9386329483548295 1103 30-31 -0.16562361021773597 1103 32-33 -4.719833218345624 1103 34-35 -1.0740241340567422 1103 36-37 -0.012870665422944683 1103 38-39 -1.7283493984990876 1103 40-41 0.605116583748611 1103 42-43 -0.24832241112118325 1103 44-45 -5.592289335474405 1103 46-47 -1.631971838040954 1103 48-49 -1.053500543200407 1103 50-51 -0.6336424513012169 1103 52-53 -0.8092882208294867 1103 54-55 -0.05922130302148432 1103 56-57 -0.6127346520555257 1103 58-59 -0.28371036370322145 1103 60-61 -0.463082409550033 1103 62-63 0.059509655543443785 1103 64-65 -0.9457400260906894 1103 66-67 -0.0884227571401972 1103 68-69 -3.597692412933693 1103 70-71 -0.9093728190977117 1103 72-73 0.9188355456451838 1103 74-75 -0.25888962603640664 1103 76 -1.3336495147256038 1104 1 -3.7441309389443376 1104 2 -1.8169743985087443 1104 3 -1.548147257719016 1104 4 -2.1033628169336325 1104 5 -1.2636100298912964 1104 6 -1.4845642064011848 1104 7 -2.246207596255882 1104 8 -1.4226055758913958 1104 9 -0.6579707177201932 1104 10-11 -1.5255711187759218 1104 12-13 -2.8554114899480183 1104 14-15 -1.4531019561672203 1104 16-17 -3.131914179476844 1104 18-19 -1.6967665365996183 1104 20-21 -0.07834105126178414 1104 22-23 -1.5067121177860017 1104 24-25 -2.141913024123159 1104 26-27 -0.8752469737162762 1104 28-29 -1.5706559779359281 1104 30-31 -2.0464058218516463 1104 32-33 -2.6307921224552153 1104 34-35 -1.0098986625459254 1104 36-37 -1.1288126944084524 1104 38-39 -1.7401619853563481 1104 40-41 -1.4445161341390182 1104 42-43 -1.6219575113792715 1104 44-45 -4.016450542542103 1104 46-47 -0.8343740615867148 1104 48-49 -1.301961928945893 1104 50-51 -0.9253835670447152 1104 52-53 -1.2151845877145355 1104 54-55 -1.709012845606356 1104 56-57 -1.7131912730600938 1104 58-59 -0.6234959503619457 1104 60-61 -1.2340770492164985 1104 62-63 -0.9474389249608279 1104 64-65 -1.3239413363944408 1104 66-67 -1.2352363366518055 1104 68-69 -4.571387072453245 1104 70-71 -2.981737321777878 1104 72-73 -0.6453891913014083 1104 74-75 -0.9459057070369994 1104 76 -1.6052397470067241 1105 1 0.14736410300837122 1105 2 -0.5944343527421552 1105 3 0.6293889741650425 1105 4 -0.12205084134247812 1105 5 0.07315579772121339 1105 6 -0.15809586162620448 1105 7 -0.49296579610332714 1105 8 -0.31162159419803004 1105 9 0.0315792441409819 1105 10-11 -0.8364978922313 1105 12-13 -2.7294580193149116 1105 14-15 -0.22855962207111347 1105 16-17 -0.28828336330598603 1105 18-19 -1.0823500606270784 1105 20-21 0.16555673668634796 1105 22-23 0.740332123251374 1105 24-25 -0.3484347632535929 1105 26-27 -0.9847054023966706 1105 28-29 -0.14149884597559392 1105 30-31 -0.5247620384801763 1105 32-33 0.1060499828079422 1105 34-35 -0.8841358860394415 1105 36-37 0.14502407141915796 1105 38-39 -0.6956349067903638 1105 40-41 -0.30845587479500836 1105 42-43 -0.10574851061649682 1105 44-45 0.6323862232855078 1105 46-47 -0.0879019029292003 1105 48-49 -0.5306995338276614 1105 50-51 -0.6076871520942966 1105 52-53 -1.4391548394307847 1105 54-55 -0.10031719343244205 1105 56-57 -0.7877526765688643 1105 58-59 -0.17240899384020736 1105 60-61 -0.7094775068824006 1105 62-63 0.2583200376631254 1105 64-65 -0.573083212824649 1105 66-67 -0.5624674579332734 1105 68-69 1.1722246743049531 1105 70-71 -0.09529567418062257 1105 72-73 -0.3022351104471035 1105 74-75 0.3355588238554468 1105 76 0.6275977815973945 1106 1 -0.810840231356952 1106 2 -0.5983043217824005 1106 3 -0.811787496423193 1106 4 -1.5856731323641533 1106 5 -0.7921692796781663 1106 6 -0.5745045303568546 1106 7 0.4675605196861454 1106 8 -1.383603018346637 1106 9 -0.11470558558038135 1106 10-11 -0.04315423898052373 1106 12-13 0.41334383827022947 1106 14-15 0.08142489805272746 1106 16-17 0.9098590515546974 1106 18-19 -0.6373036209985976 1106 20-21 0.6810366128473397 1106 22-23 -0.3927948117331468 1106 24-25 -1.0175524103124154 1106 26-27 0.2966413468293325 1106 28-29 -0.5068239233749736 1106 30-31 -0.6412481065916324 1106 32-33 0.6339137598977267 1106 34-35 -0.5110708705595606 1106 36-37 0.19301168751822928 1106 38-39 0.32100596008269733 1106 40-41 0.7627515555455524 1106 42-43 -0.0531169316691269 1106 44-45 1.9965503099728181 1106 46-47 1.4844665181234298 1106 48-49 1.019223066791536 1106 50-51 0.5018329717447116 1106 52-53 -1.0502229508858925 1106 54-55 0.8261533948028514 1106 56-57 1.0539655896850064 1106 58-59 0.8496498296892057 1106 60-61 1.248339830578896 1106 62-63 0.3117256104185415 1106 64-65 0.5782325766490359 1106 66-67 0.9661703129645609 1106 68-69 1.2647169343668736 1106 70-71 1.434503087429281 1106 72-73 0.2840651991504224 1106 74-75 0.26125541828269405 1106 76 0.4983408156531226 1107 1 0.9521009451136351 1107 2 0.2734603840999519 1107 3 -0.6739443591682921 1107 4 -0.13292803432493727 1107 5 0.9850856222826181 1107 6 0.5513778225843247 1107 7 -0.7424394803138554 1107 8 0.5252205110651289 1107 9 -2.7466663698941076 1107 10-11 0.5300810551371207 1107 12-13 -0.697440475455263 1107 14-15 -0.6409280431237434 1107 16-17 0.7475061103782252 1107 18-19 0.7664218691974831 1107 20-21 0.9706444559845941 1107 22-23 0.35357773728646436 1107 24-25 1.1348985700797414 1107 26-27 1.1763472291822765 1107 28-29 0.22876431191914293 1107 30-31 0.44953620713386044 1107 32-33 1.38254121087812 1107 34-35 0.6831448157149467 1107 36-37 -0.9795373320896132 1107 38-39 0.5404177247885791 1107 40-41 0.36794763397692165 1107 42-43 0.5902164016642075 1107 44-45 0.04615815311007054 1107 46-47 0.5711331847900958 1107 48-49 -0.8292083057574864 1107 50-51 1.0038917952741215 1107 52-53 0.6874241079376375 1107 54-55 0.7396828065675578 1107 56-57 0.9001420602732431 1107 58-59 2.04925767282646 1107 60-61 -0.40509154197012265 1107 62-63 -0.7972939974245961 1107 64-65 -0.2117674233509632 1107 66-67 0.7357781561018122 1107 68-69 1.5935404637786377 1107 70-71 1.387248185468497 1107 72-73 -0.1616210753593812 1107 74-75 -0.032862228776131985 1107 76 0.28461532545704316 1108 1 -1.6289516864653137 1108 2 -1.160223826426364 1108 3 -1.9758741837296938 1108 4 -0.2799455781845843 1108 5 -0.7031599917524716 1108 6 -1.2765169142577832 1108 7 -1.2166500066296457 1108 8 -0.7589900152506637 1108 9 -1.5736839137537544 1108 10-11 -0.7772873659155088 1108 12-13 0.09948934910614327 1108 14-15 -1.5246122536500586 1108 16-17 -1.261967573832301 1108 18-19 -0.957350060627082 1108 20-21 -1.0449695791031282 1108 22-23 -1.6544047188538897 1108 24-25 -1.6576452895693805 1108 26-27 -1.0636527708177255 1108 28-29 -0.6612356880808576 1108 30-31 -0.7550251963749126 1108 32-33 0.020523667018469638 1108 34-35 -0.6275569386710167 1108 36-37 -0.05234434963347212 1108 38-39 -0.2087928015272098 1108 40-41 -0.7031927169002721 1108 42-43 0.05872517359403062 1108 44-45 -0.5189295661881737 1108 46-47 -0.2129019029292003 1108 48-49 -0.8004363759329216 1108 50-51 0.22126021632675474 1108 52-53 -0.643102207851836 1108 54-55 -0.3108435092219146 1108 56-57 -0.45222636077938816 1108 58-59 -1.1724089938402074 1108 60-61 -0.33447750688240063 1108 62-63 -1.5245746991789844 1108 64-65 -0.6520305812457003 1108 66-67 -0.4506253526701123 1108 68-69 -0.22251216780031058 1108 70-71 0.4770727468720075 1108 72-73 -0.5851298472892061 1108 74-75 -0.9473359129866594 1108 76 -2.069770639455239 1109 1 0.4152893509107365 1109 2 -0.04885845647975984 1109 3 1.3648962205418549 1109 4 0.12852124103738305 1109 5 0.14218707155797716 1109 6 1.1386241993959167 1109 7 -0.5215699150964639 1109 8 0.5339161632390415 1109 9 1.13913073155517 1109 10-11 2.2642839536878476 1109 12-13 1.347487060776622 1109 14-15 0.822260362673358 1109 16-17 0.07823074805938646 1109 18-19 0.8756972315163232 1109 20-21 1.5955719922164775 1109 22-23 1.5150270126487797 1109 24-25 0.7711304541377118 1109 26-27 2.0812747654141575 1109 28-29 0.7119527177162439 1109 30-31 1.0260579462642951 1109 32-33 1.1228310659505816 1109 34-35 1.6277824968743673 1109 36-37 2.5523467258813994 1109 38-39 1.774330768266843 1109 40-41 1.439541836875474 1109 42-43 0.8925352422439197 1109 44-45 0.7154335154289129 1109 46-47 -0.5735044963693241 1109 48-49 1.4806467667062826 1109 50-51 1.349978751795863 1109 52-53 1.270322658662277 1109 54-55 -0.3322012514034576 1109 56-57 0.5984029298384606 1109 58-59 -0.04378580543441046 1109 60-61 1.2079519362907476 1109 62-63 0.269952379387 1109 64-65 0.5093919969388914 1109 66-67 2.0473723590003665 1109 68-69 2.015569449285884 1109 70-71 2.612465576772845 1109 72-73 1.8546108086985882 1109 74-75 2.5903261770209696 1109 76 -0.576254239760349 1110 1 0.5893982424109332 1110 2 1.3810279516675195 1110 3 1.163172757948825 1110 4 1.6531980918011904 1110 5 1.745986523183518 1110 6 1.6043507955572949 1110 7 2.6635064656320893 1110 8 4.303598889443506 1110 9 -0.22162132484906039 1110 10-11 0.6362972713533388 1110 12-13 2.592199164184372 1110 14-15 1.9355584433627406 1110 16-17 2.230208813080928 1110 18-19 2.4557011484767592 1110 20-21 0.6353291406692776 1110 22-23 2.186100259808981 1110 24-25 2.597511182692358 1110 26-27 -0.31365277081772547 1110 28-29 1.3117372848921178 1110 30-31 2.6461127837104357 1110 32-33 2.2610997694366795 1110 34-35 1.880622293192424 1110 36-37 1.6560482534959746 1110 38-39 1.0936609680318234 1110 40-41 1.0558755619048519 1110 42-43 1.2509371223849293 1110 44-45 1.977869864821784 1110 46-47 0.7277097613666719 1110 48-49 1.8236745771253986 1110 50-51 1.0779458493281808 1110 52-53 0.6890457295592576 1110 54-55 0.8388719957567439 1110 56-57 1.4106826008137823 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2116 66-67 -0.38543396511030537 2116 68-69 -0.1446413544031806 2116 70-71 -2.3315396933193817 2116 72-73 0.6517122579739514 2116 74-75 -1.1419531378670413 2116 76 -1.1032634624217437 2117 1 0.606915759928448 2117 2 1.0912381618777296 2117 3 0.8238334186094853 2117 4 -0.04700210839900976 2117 5 -1.95121067401368 2117 6 -0.5908443996378985 2117 7 -1.2824394803138546 2117 8 -1.0377424518978344 2117 9 0.9555558523281178 2117 10-11 0.16082179587786172 2117 12-13 1.1192261912114034 2117 14-15 0.6527756605799588 2117 16-17 0.19417277704489067 2117 18-19 0.8338292766048916 2117 20-21 0.2747185300586672 2117 22-23 -0.623459299750575 2117 24-25 0.7534170885982583 2117 26-27 1.2789398217748698 2117 28-29 0.2554309785858102 2117 30-31 -0.1341674965698445 2117 32-33 1.9340226923596013 2117 34-35 1.329070741640873 2117 36-37 -0.5499077024599828 2117 38-39 1.211528835899692 2117 40-41 -1.5664968104675232 2117 42-43 -1.1734873020394971 2117 44-45 0.7661581531100694 2117 46-47 -0.5618297781728678 2117 48-49 0.39449539794621913 2117 50-51 1.2646325360148651 2117 52-53 1.1044611449746746 2117 54-55 -1.2021690452842932 2117 56-57 -0.8798579397267581 2117 58-59 -0.17703862346983712 2117 60-61 0.9430566061780254 2117 62-63 -0.07988659001718901 2117 64-65 -1.4773229789065212 2117 66-67 -1.643851473527814 2117 68-69 0.4994663897045619 2117 70-71 0.47317411139442456 2117 72-73 1.2998604061221002 2117 74-75 1.467137771223868 2117 76 -0.22279208195036304 2118 1 -0.7078990548863651 2118 2 -0.7382637538310846 2118 3 -0.5171627499728899 2118 4 -1.8062613676582693 2118 5 -2.3560638030047407 2118 6 -0.47207045327774466 2118 7 -0.6445084458310966 2118 8 -2.109262247555563 2118 9 -3.082758323917094 2118 10-11 -1.6747465310697756 2118 12-13 -0.5704289812023902 2118 14-15 -1.7664452845030532 2118 16-17 -1.4293904413459089 2118 18-19 -0.6540379009174622 2118 20-21 -3.4832635899924185 2118 22-23 -1.9944682397250304 2118 24-25 -1.8656761425639345 2118 26-27 -1.5464113915073803 2118 28-29 -2.290546032908445 2118 30-31 -1.2523028733259096 2118 32-33 -1.544585225903493 2118 34-35 -0.6284643796873546 2118 36-37 -1.573215493009151 2118 38-39 -1.2013064131424542 2118 40-41 -1.961132825793193 2118 42-43 -1.3548410696001625 2118 44-45 -0.2711981687290077 2118 46-47 -2.8517403784282926 2118 48-49 -2.754610604608061 2118 50-51 -2.2519702736913914 2118 52-53 -3.0360241679244346 2118 54-55 -1.1347999520531324 2118 56-57 -0.31951311214055167 2118 58-59 -0.538788304185033 2118 60-61 -1.572907633924146 2118 62-63 -1.9671790548958583 2118 64-65 -1.6028019061095833 2118 66-67 -2.775716096771749 2118 68-69 -1.0212871224282587 2118 70-71 -1.7688437685544898 2118 72-73 -3.150011879957084 2118 74-75 -1.8604484356726836 2118 76 -1.976304214772842 2119 1 1.080562483575175 2119 2 -0.7826934620538957 2119 3 0.8024658972419658 2119 4 -0.28703059842749923 2119 5 0.2938035710005664 2119 6 0.5736855148920164 2119 7 -1.0709010187753947 2119 8 1.2713743572189742 2119 9 0.34230798908025406 2119 10-11 0.45854259359866134 2119 12-13 0.30191849890371003 2119 14-15 -0.04131265850835675 2119 16-17 -0.13595542808331373 2119 18-19 0.7405244333000489 2119 20-21 -0.7640991337589966 2119 22-23 1.2276803013890287 2119 24-25 0.0650267752079472 2119 26-27 -1.5348066169715722 2119 28-29 -0.5266203034654708 2119 30-31 0.018254155851806786 2119 32-33 -0.707715199378292 2119 34-35 -0.1417269791568465 2119 36-37 -0.6763322038844848 2119 38-39 -1.697659198288342 2119 40-41 -1.1829498019205147 2119 42-43 0.17765229910010305 2119 44-45 -0.4678162058642883 2119 46-47 0.2633126719695831 2119 48-49 -1.431003177552359 2119 50-51 -0.5049543585720286 2119 52-53 -1.0671912766777503 2119 54-55 0.14968280656755795 2119 56-57 0.19706513719632213 2119 58-59 0.6352833138520992 2119 60-61 0.25965204777346784 2119 62-63 0.17937266924207051 2119 64-65 0.32823257664903593 2119 66-67 -0.267426972103312 2119 68-69 -2.1091518439136685 2119 70-71 -2.2187774555571416 2119 72-73 -0.6079031266414354 2119 74-75 0.3132916173777147 2119 76 0.7551281459698629 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 15.0 13 107.0 14 333.0 15 612.0 16 796.0 17 922.0 18 964.0 19 885.0 20 762.0 21 612.0 22 516.0 23 410.0 24 383.0 25 375.0 26 387.0 27 437.0 28 544.0 29 589.0 30 684.0 31 860.0 32 999.0 33 1330.0 34 1672.0 35 2436.0 36 3627.0 37 2995.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.378580323785805 21.847239518472396 15.828144458281447 29.946035699460356 2 16.455696202531644 22.158083536057397 30.16121716901002 31.22500309240094 3 18.983218570898448 25.10617243227642 28.26866779367501 27.64194120315013 4 11.243969818166825 26.41735043087453 31.356945532511443 30.981734218447205 5 10.489423988784893 32.045520141838125 30.48695006803282 26.978105801344164 6 25.328825299962894 34.70498495031543 17.568960540964003 22.39722920875768 7 20.20368614192059 30.44571805549829 18.62862326310147 30.72197253947965 8 17.60194615099163 33.03096524141343 17.568960540964003 31.79812806663093 9 20.446955015874323 21.523110543025606 18.781181709479238 39.248752731620826 10-11 19.45326351379211 26.240052776976043 25.629818991464976 28.676864717766875 12-13 19.58520595390261 24.35574980414794 25.341194903723252 30.7178493382262 14-15 19.0883602028615 26.233867975095865 23.036325403042923 31.641446418999713 16-17 20.78711911928421 28.00890611470746 23.547602358471117 27.656372407537212 18-19 19.26978105801344 26.80905454995258 24.02383210324496 29.897332288789013 20-21 18.420401599802087 27.388364326062753 24.48975384488517 29.701480229249988 22-23 21.395291304168556 27.648126005030306 23.32082628953119 27.635756401269944 24-25 19.333690677441968 29.7179730342638 23.374427905826085 27.573908382468147 26-27 17.81429101554447 30.571475693728612 25.460767740073393 26.153465550653525 28-29 20.005772481754835 28.132602152311055 27.085309033933946 24.776316332000164 30-31 19.4450171112852 27.64194120315013 27.244052282191895 25.668989403372777 32-33 17.700902981074506 30.58384529748897 26.155527151280257 25.559724570156266 34-35 18.572960046179855 29.12629365439327 26.10398713561209 26.196759163814786 36-37 18.904877747082836 28.270729394301735 26.219436770708775 26.60495608790665 38-39 19.01620418092607 27.0894322351874 27.64400280377685 26.250360780109677 40-41 17.89675504061353 26.856471364367295 27.36774831979549 27.879025275223686 42-43 16.783490702181172 26.650311301694636 28.4542118500804 28.111986146043787 44-45 16.64536346019049 26.73896012864388 29.299468107038308 27.316208304127326 46-47 17.653486166659796 27.392487527316206 28.00890611470746 26.94512019131654 48-49 16.46600420566528 28.92013359172061 26.68535851234899 27.92850369026512 50-51 16.70721147899229 27.662557209417393 26.747206531150784 28.88302478043953 52-53 15.82484641075331 27.79037644827444 27.097678637694305 29.287098503277946 54-55 15.973281655877624 27.722343627592462 26.514245660330683 29.790129056199234 56-57 16.946357151692574 28.027460520347997 25.953490289861048 29.07269203809838 58-59 17.14014761060487 27.000783408238156 26.396734424607267 29.462334556549706 60-61 16.03100647342597 26.52455366346431 26.475075248422876 30.969364614686846 62-63 16.843277120356245 27.3450707129015 25.747330227188385 30.064321939553867 64-65 17.46175730837422 27.303838700366967 24.504185049272255 30.73021894198656 66-67 17.084484393683255 28.874778377932625 23.45895353152187 30.581783696862242 68-69 17.925617449387705 28.445965447573496 23.992908093844058 29.635509009194738 70-71 18.98115697027172 28.4232878406795 23.815610439945573 28.779944749103205 72-73 19.352245083082504 28.577907887683995 23.45895353152187 28.610893497711626 74-75 18.698717684410177 28.880963179812806 23.08580381808436 29.334515317692656 76 18.608007256834206 27.23786748031171 24.091864923926938 30.062260338927143 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 1.5 14 3.0 15 2.0 16 2.5 17 4.0 18 4.0 19 5.0 20 9.5 21 13.0 22 14.5 23 27.5 24 38.5 25 38.0 26 48.0 27 79.0 28 112.0 29 124.0 30 139.5 31 193.5 32 263.5 33 295.0 34 345.5 35 462.5 36 574.5 37 620.0 38 705.0 39 909.5 40 1156.0 41 1331.0 42 1379.0 43 1444.5 44 1579.5 45 1663.5 46 1678.0 47 1724.5 48 1691.0 49 1598.5 50 1586.0 51 1460.5 52 1252.0 53 1088.0 54 1007.0 55 920.5 56 758.5 57 625.5 58 568.0 59 503.5 60 382.0 61 306.0 62 287.0 63 239.0 64 159.0 65 118.5 66 94.5 67 79.0 68 58.0 69 32.0 70 27.0 71 27.0 72 22.0 73 17.5 74 13.0 75 8.0 76 7.0 77 5.0 78 5.0 79 6.0 80 4.5 81 2.5 82 3.0 83 4.0 84 2.5 85 1.5 86 1.5 87 1.0 88 1.0 89 0.5 90 1.0 91 1.5 92 1.0 93 1.0 94 1.0 95 1.5 96 2.0 97 1.5 98 2.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6720818043128686 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 24253.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.04514905372531 #Duplication Level Percentage of deduplicated Percentage of total 1 88.64055076117522 75.3844885168845 2 7.844468146998933 13.342679256174495 3 2.1283816542228258 5.43025605079784 4 0.707844468146999 2.4079495320166577 5 0.3733152332008145 1.5874324825794748 6 0.1551439930185203 0.7916546406630108 7 0.07757199650926015 0.4617985403867563 8 0.033937748472801316 0.23089927019337814 9 0.009696499563657519 0.07421762256215726 >10 0.02908949869097256 0.28862408774172266 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004123201253453181 0.0 2 0.0 0.0 0.0 0.004123201253453181 0.0 3 0.0 0.0 0.0 0.004123201253453181 0.0 4 0.0 0.0 0.0 0.008246402506906362 0.0 5 0.0 0.0 0.0 0.012369603760359543 0.0 6 0.0 0.0 0.0 0.012369603760359543 0.0 7 0.0 0.0 0.0 0.012369603760359543 0.0 8 0.0 0.0 0.0 0.012369603760359543 0.0 9 0.0 0.0 0.0 0.012369603760359543 0.0 10 0.0 0.0 0.0 0.012369603760359543 0.0 11 0.0 0.0 0.0 0.016492805013812725 0.0 12 0.0 0.0 0.0 0.016492805013812725 0.0 13 0.0 0.0 0.0 0.016492805013812725 0.0 14 0.0 0.0 0.0 0.016492805013812725 0.0 15 0.0 0.0 0.0 0.016492805013812725 0.0 16 0.0 0.0 0.0 0.03298561002762545 0.0 17 0.0 0.0 0.0 0.04123201253453181 0.0 18 0.0 0.0 0.0 0.04535521378798499 0.0 19 0.0 0.0 0.0 0.04535521378798499 0.0 20 0.0 0.0 0.0 0.04947841504143817 0.0 21 0.0 0.0 0.0 0.04947841504143817 0.0 22 0.0 0.0 0.0 0.04947841504143817 0.0 23 0.0 0.0 0.0 0.04947841504143817 0.0 24 0.0 0.0 0.0 0.0659712200552509 0.0 25 0.0 0.0 0.0 0.07834082381561044 0.0 26 0.0 0.0 0.0 0.08658722632251681 0.0 27 0.0 0.0 0.0 0.09895683008287634 0.0 28 0.0 0.0 0.0 0.09895683008287634 0.0 29 0.0 0.0 0.0 0.11132643384323589 0.0 30 0.0 0.0 0.0 0.13606564136395496 0.0 31 0.0 0.0 0.0 0.17729765389848678 0.0 32 0.0 0.0 0.0 0.2556384777140972 0.0 33 0.0 0.0 0.0 0.3133632952624418 0.0 34 0.0 0.0 0.0 0.38345771657114586 0.0 35 0.0 0.0 0.0 0.4576753391333031 0.0 36 0.0 0.0 0.0 0.6102337855110708 0.0 37 0.0 0.0 0.0 0.8823650682389808 0.0 38 0.0 0.0 0.0 1.1132643384323588 0.0 39 0.0 0.0 0.0 1.4183812311878943 0.0 40 0.0 0.0 0.0 1.8554405640539315 0.0 41 0.0 0.0 0.0 2.4079495320166577 0.0 42 0.0 0.0 0.0 3.0181833175277286 0.0 43 0.0 0.0 0.0 3.558322681730095 0.0 44 0.0 0.0 0.0 4.180926071001526 0.0 45 0.0 0.0 0.0 4.807652661526409 0.0 46 0.0 0.0 0.0 5.343668824475323 0.0 47 0.0 0.0 0.0 5.991011421267472 0.0 48 0.0 0.0 0.0 6.617738011792356 0.0 49 0.0 0.0 0.0 7.133138168474003 0.0 50 0.0 0.0 0.0 7.607306312621119 0.0 51 0.0 0.0 0.0 8.176308085597658 0.0 52 0.0 0.0 0.0 8.77417226734837 0.0 53 0.0 0.0 0.0 9.487486084195769 0.0 54 0.0 0.0 0.0 10.056487857172309 0.0 55 0.0 0.0 0.0 10.56364161134705 0.0 56 0.0 0.0 0.0 11.190368201871934 0.0 57 0.0 0.0 0.0 11.697521956046675 0.0 58 0.0 0.0 0.0 12.126334886405806 0.0 59 0.0 0.0 0.0 12.61287263431328 0.0 60 0.0 0.0 0.0 13.09116397971385 0.0 61 0.0 0.0 0.0 13.5447161175937 0.0 62 0.0 0.0 0.0 14.080732280542613 0.0 63 0.0 0.0 0.0 14.571393229703542 0.0 64 0.0 0.0 0.0 15.111532593905908 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGATC 30 8.1378275E-6 58.33162 35 TGGTTAT 25 2.3448499E-4 55.998352 16 GGTTATT 20 0.0068714656 52.49845 17 ATCGTGT 20 0.0068714656 52.49845 30 TTTACAA 20 0.0068714656 52.49845 34 ATGGTTA 30 5.7665096E-4 46.665295 15 CGTGTAG 30 5.7665096E-4 46.665295 32 GTGTAGA 45 0.0042272164 31.110197 33 CGACGAT 95 0.007015199 18.42051 70 CCGACGA 145 0.0045657936 14.482332 18 ACGCTGC 225 0.00638811 10.888568 12 >>END_MODULE