FastQCFastQC Report
Wed 25 May 2016
SRR1780522_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780522_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24253
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT14535.991011421267472No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT12545.170494371830289TruSeq Adapter, Index 8 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC3941.6245412938605535No Hit
CTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC2040.8411330557044489No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG1530.6308497917783368No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGT1100.45355213787984994No Hit
TATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCT1030.42468972910567765No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGT630.25976167896755037No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC610.25151527646064403No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC600.24739207520719086No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC570.23502247144683133No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGTCTT550.22677606893992497No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGTCTT530.2185296664330186No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG480.1979136601657527No Hit
CTTATAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG360.14843524512431452No Hit
CTTAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC350.14431204387086133No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAACCTCGTATGCCGT350.14431204387086133No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAACCTCGTATGCCGTCTT290.11957283635014226No Hit
TTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC270.11132643384323589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACAC550.063.636362
CAAACAC200.00687066552.564
CTATACA552.1318556E-950.909091
TTACACA751.2732926E-1146.6666683
CTTTACA703.5288394E-1045.01
AACTCGT602.4622204E-740.83333237
GAAACTC556.4322758E-638.18181635
CGAAACT556.4322758E-638.18181634
ACTCGTA708.2427505E-735.038
AAACTCG601.1682745E-535.036
GTCGAAA601.1682745E-535.032
TCGAAAC652.0208803E-532.30769333
CTCGTAT6800.029.85294339
TTCTGCT7100.029.57746552
CTTCTGC7100.029.57746551
AATCTCG5700.029.47368436
TCTTCTG7150.029.37062850
ATCTCGT5850.029.3162437
TCTCGTA5750.029.21739238
GAATCTC5750.029.21739235
Produced by FastQC (version 0.11.5)