FastQCFastQC Report
Wed 25 May 2016
SRR1780521_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780521_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275233
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG250.002331106735.2568519
TAAGCAC250.002356227735.17992831
GTAATTT353.235984E-431.3878213
CTTACGC300.005712619529.3593112
GTCATAC300.005743194429.3272691
CTATACA804.294634E-927.4943161
CTTATAA407.026712E-427.4943161
GAACAAC407.026712E-427.4943161
TCGGTAA407.041672E-427.48431827
CGGATCC701.1112024E-625.18346415
ATTTGCG1151.2732926E-1124.85538530
GAAAATT1350.024.43050626
TTCTACA450.001404573624.4171872
GCGTGTA1202.3646862E-1123.84141734
AGTAAAG651.8020495E-523.70034434
ACCTTAA651.8147855E-523.67879732
CCGGATC752.0259758E-623.50456614
GGATCCA752.0259758E-623.50456616
TCCACAA752.0648458E-623.45328326
TTAATAC500.002594897321.9714763
Produced by FastQC (version 0.11.5)