FastQCFastQC Report
Wed 25 May 2016
SRR1780521_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780521_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275233
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT229888.35219613927109No Hit
ATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTT182936.646368713054031RNA PCR Primer, Index 17 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC48591.765413304363939No Hit
CTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC28501.035486297064669No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG28361.0303996977106669No Hit
TATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCT17150.6231084208652306RNA PCR Primer, Index 17 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC14690.5337296036449118No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC9370.34043882819284027No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG7980.2899361631781073No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC6910.25106001097252145No Hit
CTTAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC5560.2020106600589319No Hit
CTTATAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG5120.18602420494635455No Hit
TTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC3730.13552153993162155No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG3690.13406822583047817No Hit
CTTATTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG3510.1275283123753329No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCG207.846354E-443.99963827
CCGTCTT19100.042.27190444
GCCGTCT20950.038.53906243
CTATACA3650.036.1772271
TCTTAGA250.002349741835.1997124
AGCGAAA250.002349741835.1997130
TTTACAC6500.032.8304982
CTTTACA6600.032.678271
TTACACA6550.032.5798843
ATAACAC950.032.4207844
CTTTATA1650.031.9997352
CTTATAA900.031.7890681
GGTACAA353.204772E-431.4397351
GGCAAGT353.2116126E-431.42831238
TCTTTAT1700.031.0698571
CTTATAC35350.030.1323411
TTATACA35750.029.415142
GCGCGTA300.005737636729.3330929
CACGCTA300.005737636729.33309223
TGCGGCA300.005737636729.33309235
Produced by FastQC (version 0.11.5)