FastQCFastQC Report
Wed 25 May 2016
SRR1780520_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780520_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83611
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG150.00221857769.9928359
ACATTCG150.00221857769.9928310
ATCCAGT150.00221857769.9928345
AACTAGC150.00221857769.9928338
CATCCGG150.00221857769.992838
AAGGCAA150.00221857769.992837
ATCCAAC200.006737579652.8740961
GATTATA200.006737579652.8740961
AACGTTG200.00693243352.49461724
CGGCGGC200.00693243352.49461725
GGCAAGC200.00693243352.49461727
GTTAGTG200.00693243352.49461731
CTGACAT200.00693243352.4946174
ATCCGGC200.00693243352.4946179
ACTAGCC200.00693243352.49461739
AGGCAAG200.00693243352.4946178
CATCGCG200.00693243352.4946178
CCAACGT200.00693243352.49461722
CGCAAAG200.00693243352.49461712
TTTTCGC200.00693243352.4946179
Produced by FastQC (version 0.11.5)