##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780520_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 83611 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.044384112138356 34.0 24.0 34.0 12.0 34.0 2 30.082453265718627 34.0 31.0 34.0 12.0 34.0 3 30.263398356675555 34.0 32.0 34.0 11.0 34.0 4 30.32010142206169 34.0 32.0 34.0 11.0 34.0 5 30.54962863738025 34.0 32.0 34.0 21.0 34.0 6 33.337635000179404 38.0 34.0 38.0 21.0 38.0 7 32.61153436748753 37.0 32.0 38.0 11.0 38.0 8 31.702575020033247 37.0 27.0 38.0 11.0 38.0 9 32.44510889715468 37.0 31.0 38.0 12.0 38.0 10-11 32.63736230878712 37.0 32.5 38.0 11.0 38.0 12-13 32.92491418593248 37.0 33.5 38.0 11.0 38.0 14-15 32.59741541184772 37.0 32.0 38.0 11.0 38.0 16-17 32.44057600076545 37.0 31.0 38.0 11.0 38.0 18-19 32.4556457882336 37.0 32.0 38.0 11.0 38.0 20-21 32.50910167322481 37.0 31.5 38.0 11.0 38.0 22-23 32.4321082154262 37.0 31.5 38.0 11.0 38.0 24-25 32.8099891162646 37.5 33.5 38.0 11.0 38.0 26-27 32.795451555417344 37.5 33.5 38.0 11.0 38.0 28-29 32.84297520661157 37.5 34.0 38.0 11.0 38.0 30-31 32.71089928358709 37.0 34.0 38.0 11.0 38.0 32-33 32.52630036717657 37.0 32.0 38.0 11.0 38.0 34-35 32.47532621305809 37.0 31.5 38.0 11.0 38.0 36-37 32.420166006865124 37.0 31.5 38.0 11.0 38.0 38-39 32.80698711891976 37.0 34.0 38.0 11.0 38.0 40-41 32.44867302149238 37.0 31.5 38.0 11.0 38.0 42-43 32.72579564889787 37.0 33.5 38.0 11.0 38.0 44-45 31.87422707538482 37.0 29.0 38.0 11.0 38.0 46-47 32.52691631483896 37.0 32.0 38.0 11.0 38.0 48-49 32.39658657353698 37.0 31.0 38.0 11.0 38.0 50-51 32.305671502553494 37.0 31.5 38.0 11.0 38.0 52-53 32.33321572520362 37.0 31.0 38.0 11.0 38.0 54-55 32.07518747533219 37.0 31.0 38.0 11.0 38.0 56-57 32.07872169929794 37.0 31.0 38.0 11.0 38.0 58-59 31.9681022831924 37.0 29.0 38.0 11.0 38.0 60-61 32.000998672423485 37.0 31.0 38.0 11.0 38.0 62-63 32.06938082309744 37.0 31.0 38.0 11.0 38.0 64-65 32.12848189831482 37.0 31.0 38.0 11.0 38.0 66-67 32.10533901041729 37.0 31.0 38.0 11.0 38.0 68-69 31.585239980385357 37.0 28.5 38.0 11.0 38.0 70-71 31.452069703747114 37.0 27.0 38.0 11.0 38.0 72-73 31.517712980349476 37.0 27.5 38.0 11.0 38.0 74-75 31.19890923443088 37.0 27.0 38.0 11.0 38.0 76 30.5413880948679 36.0 25.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -3.69915949931163 1101 2 -1.6737518659514663 1101 3 -0.7342091190660689 1101 4 -2.068036422800649 1101 5 -0.7900177564493411 1101 6 -3.0137048873253356 1101 7 -1.3934563315863748 1101 8 -1.2629743787218928 1101 9 -2.2879241093292464 1101 10-11 -1.8137280943964456 1101 12-13 -1.8199894837998407 1101 14-15 -1.0684119944859347 1101 16-17 -2.490330397306984 1101 18-19 -0.7114827343255499 1101 20-21 -1.5498798109396716 1101 22-23 -0.4107734476717866 1101 24-25 -1.4847046483092257 1101 26-27 -1.3333919375959624 1101 28-29 -0.4820527149737259 1101 30-31 -1.8324523198330738 1101 32-33 -0.8640222565359572 1101 34-35 -1.4736594153619222 1101 36-37 -0.5384269585875927 1101 38-39 -0.9246170394892665 1101 40-41 0.15950911106695997 1101 42-43 -1.067584729329262 1101 44-45 -0.9180313715658528 1101 46-47 0.2580906735581294 1101 48-49 -0.9546298566425122 1101 50-51 -0.8272131182920894 1101 52-53 -0.37856514233098437 1101 54-55 -1.3533951126526986 1101 56-57 -1.1148082450837151 1101 58-59 -0.8463168233445124 1101 60-61 -0.5974707468424931 1101 62-63 -2.295389890236887 1101 64-65 -0.5310159407781114 1101 66-67 -1.2591024528555224 1101 68-69 -0.13974225401725704 1101 70-71 -0.42537129415978114 1101 72-73 -0.4363723888951867 1101 74-75 -1.4921393128704565 1101 76 -1.7719930713904901 1102 1 -0.2629774793338271 1102 2 -0.674214315082061 1102 3 0.307503792513657 1102 4 -0.8737152981243632 1102 5 -0.24257047565022916 1102 6 -0.30449290064387213 1102 7 0.2953154035227641 1102 8 0.617528765932196 1102 9 0.6560270560425607 1102 10-11 -0.5027772989839363 1102 12-13 -3.19276972797298 1102 14-15 -1.0894996748411003 1102 16-17 -2.6248106044841926 1102 18-19 -0.8214068926681328 1102 20-21 -0.45900855677763275 1102 22-23 -1.4929044132655669 1102 24-25 -0.4643568865630172 1102 26-27 -5.19891173041875 1102 28-29 -1.268493706464664 1102 30-31 -0.42059512412460265 1102 32-33 -6.345293066746834 1102 34-35 -1.1693678874299955 1102 36-37 -0.5266807506704652 1102 38-39 -0.25499069838678423 1102 40-41 0.11599264787790986 1102 42-43 -0.6911511370244128 1102 44-45 -4.901244468125228 1102 46-47 -1.1344361484914494 1102 48-49 -0.2741359609710372 1102 50-51 -5.9181768622802515 1102 52-53 -1.8347249647505137 1102 54-55 -0.21438290399564863 1102 56-57 -1.920903324624966 1102 58-59 0.17703685775056854 1102 60-61 -0.04650885265085236 1102 62-63 -4.1987842668554585 1102 64-65 -1.0074310351177331 1102 66-67 -1.0345371550382794 1102 68-69 -6.888817355756068 1102 70-71 -1.514115282321086 1102 72-73 -0.4033997658837194 1102 74-75 -6.163060511538603 1102 76 -2.612078162030521 1103 1 0.025326680702544735 1103 2 0.059475159798594746 1103 3 -0.2578330264603501 1103 4 0.421332818871921 1103 5 0.22687333733757242 1103 6 -0.33203703946330165 1103 7 0.2711432431832961 1103 8 1.199877026900598 1103 9 0.11634000595542204 1103 10-11 0.12576517278994714 1103 12-13 -1.13867599454872 1103 14-15 -0.8315527929740085 1103 16-17 -0.6474293814789647 1103 18-19 -0.0890813641389201 1103 20-21 -0.6786891660070005 1103 22-23 -0.36535648570627544 1103 24-25 0.09567569659982667 1103 26-27 -0.8359315076408507 1103 28-29 0.18981121131967882 1103 30-31 0.14702441061814397 1103 32-33 -4.263532060457525 1103 34-35 -1.0645523990614265 1103 36-37 0.14985558334484494 1103 38-39 -0.11526197216112877 1103 40-41 -0.0652133080211641 1103 42-43 0.10870867960057495 1103 44-45 -4.442304936413478 1103 46-47 -0.7176652914784896 1103 48-49 -0.5698849948423721 1103 50-51 -0.4267583580747498 1103 52-53 -0.4457160990992328 1103 54-55 -0.7373426675782824 1103 56-57 0.4933006611293749 1103 58-59 0.2517223923417582 1103 60-61 0.457336718312245 1103 62-63 0.19362499282476975 1103 64-65 0.47099209135040354 1103 66-67 -0.13643531371070594 1103 68-69 -2.7839071488908687 1103 70-71 0.003786513541612635 1103 72-73 0.36586597633042217 1103 74-75 -1.6420330052871819 1103 76 -0.12410741110227619 1104 1 -0.9647161351891214 1104 2 0.22824943043485746 1104 3 0.47758799647436234 1104 4 0.5783742075672009 1104 5 0.08407072191721099 1104 6 -0.06669467828304221 1104 7 1.2443252273181784 1104 8 1.5889539959337533 1104 9 0.7433106986435334 1104 10-11 -0.10968904075846098 1104 12-13 -0.3438105824872508 1104 14-15 0.4605743934576694 1104 16-17 -0.17802282965182314 1104 18-19 0.7624072348852735 1104 20-21 0.9475679284866771 1104 22-23 0.7412683607871315 1104 24-25 0.9821969883785044 1104 26-27 1.124961651773667 1104 28-29 0.18655853117762433 1104 30-31 -0.013277955872609937 1104 32-33 -0.3909425287770887 1104 34-35 0.43097516574324857 1104 36-37 1.0176822619601325 1104 38-39 0.19444794187517545 1104 40-41 0.7413434977141762 1104 42-43 0.7384839791458688 1104 44-45 -2.6183230584885564 1104 46-47 0.8922869140377827 1104 48-49 0.9054211885689583 1104 50-51 0.8753675007323807 1104 52-53 -0.06804010587011788 1104 54-55 -0.3193649716706801 1104 56-57 0.7772878495524864 1104 58-59 0.9073697752618735 1104 60-61 0.020581432707015068 1104 62-63 0.6639359888750853 1104 64-65 0.0640253815359344 1104 66-67 0.12989446822695783 1104 68-69 -3.485067160839492 1104 70-71 -0.48008284820288694 1104 72-73 0.6517616250409688 1104 74-75 -0.45956760650196316 1104 76 0.5411652497892234 1105 1 -0.5029841912437405 1105 2 -0.3060660564498008 1105 3 -0.8427497685274332 1105 4 -0.020799346151807896 1105 5 0.019212655082860408 1105 6 -0.16425127188706767 1105 7 0.6539551231140805 1105 8 -0.3160046817521973 1105 9 0.5172956070157895 1105 10-11 0.7767644267226785 1105 12-13 -1.1129446105145568 1105 14-15 -0.5417612155973082 1105 16-17 0.020054784433703787 1105 18-19 0.29672272176993175 1105 20-21 -0.8589358444202588 1105 22-23 -0.32736261298217073 1105 24-25 -0.4622873810070516 1105 26-27 -0.1095873960164866 1105 28-29 -1.0831144835546809 1105 30-31 0.37326534272601464 1105 32-33 0.04608585785604191 1105 34-35 0.17702259657328057 1105 36-37 0.49172106900289236 1105 38-39 0.3323387072362962 1105 40-41 -0.403015108106878 1105 42-43 0.21620586200609182 1105 44-45 0.6668907395710875 1105 46-47 0.267691154066533 1105 48-49 0.30696628378994717 1105 50-51 -0.3496594486028677 1105 52-53 -1.1219387763695785 1105 54-55 -0.9615676306717589 1105 56-57 -0.179144058460448 1105 58-59 -0.06909447195336682 1105 60-61 0.1517348642557046 1105 62-63 0.04949114518123565 1105 64-65 -0.25038352971091626 1105 66-67 0.3153957258626079 1105 68-69 0.477652925398381 1105 70-71 0.6189110432811233 1105 72-73 -0.03320064588968563 1105 74-75 -1.2349898127542076 1105 76 -1.7452163527979145 1106 1 -0.3727921102507423 1106 2 -0.3417085222982159 1106 3 -0.6660976639577108 1106 4 0.15822776708582964 1106 5 0.14058079876221896 1106 6 -0.7459030297401981 1106 7 0.8875034207356052 1106 8 0.8993063230324942 1106 9 1.367132650216675 1106 10-11 0.645766229648963 1106 12-13 0.4449743964891155 1106 14-15 0.039431142768961536 1106 16-17 0.8560318317951854 1106 18-19 0.11346566608724373 1106 20-21 -0.09683543529467897 1106 22-23 0.31407175356660844 1106 24-25 0.7957391081407224 1106 26-27 0.754456359617663 1106 28-29 -0.12098460351862173 1106 30-31 -0.24718400197961898 1106 32-33 1.1224328518231772 1106 34-35 0.8027853730797716 1106 36-37 0.5710931652514191 1106 38-39 0.263463455866777 1106 40-41 -0.058370145898983594 1106 42-43 0.4144844141175952 1106 44-45 1.2318654292597415 1106 46-47 1.329814099770715 1106 48-49 1.158502238300727 1106 50-51 0.359835385319883 1106 52-53 -0.1290811382030128 1106 54-55 0.2953406974608228 1106 56-57 1.1914353049678894 1106 58-59 0.13988028707860067 1106 60-61 1.1017036597622543 1106 62-63 1.21093105919552 1106 64-65 0.7781945855376762 1106 66-67 1.4309130270206438 1106 68-69 1.5360833393779956 1106 70-71 0.31392179149553456 1106 72-73 0.3047266823122463 1106 74-75 -1.1838317793307276 1106 76 -1.0101251663337294 1107 1 -0.048637128201022506 1107 2 -0.2617789686844496 1107 3 0.06278535439899358 1107 4 -0.7105150358674983 1107 5 -0.7435721511802065 1107 6 0.3479803273010518 1107 7 -0.2843451195339881 1107 8 0.11958675383923989 1107 9 -0.4701294238875029 1107 10-11 -0.35972892716198146 1107 12-13 0.1577529645308431 1107 14-15 -0.8651698868320636 1107 16-17 0.2326236491764746 1107 18-19 0.23628961785856717 1107 20-21 0.018198771844794237 1107 22-23 -0.8160085146715552 1107 24-25 -0.7109329774787305 1107 26-27 -0.25808343581687154 1107 28-29 -0.7421405338850775 1107 30-31 -0.523448988770923 1107 32-33 0.5447047477792779 1107 34-35 0.2753942601623436 1107 36-37 -0.28458488121610515 1107 38-39 0.6602493394947615 1107 40-41 0.012355655089969275 1107 42-43 -0.15194009309638545 1107 44-45 -0.5347131794241307 1107 46-47 -0.29107088295636885 1107 48-49 -0.1698524321592032 1107 50-51 0.5202109864849547 1107 52-53 0.2870605674123752 1107 54-55 -0.017544556213898943 1107 56-57 -0.11025642435541982 1107 58-59 0.426754567373937 1107 60-61 -0.9607836395671505 1107 62-63 -0.6313350791232502 1107 64-65 -0.45379586355803525 1107 66-67 0.4824184798418827 1107 68-69 0.842296507433268 1107 70-71 -0.4824441232914367 1107 72-73 -0.18976969376307906 1107 74-75 0.09234853632327145 1107 76 0.07337750927420927 1108 1 -0.799049662845924 1108 2 -0.658517141356235 1108 3 -1.1666800327427431 1108 4 -0.5341905192940182 1108 5 -0.17944904763801617 1108 6 -0.41460798715448277 1108 7 0.24333074839084645 1108 8 0.7352967139475375 1108 9 -0.7006041190924854 1108 10-11 -0.20371873795676976 1108 12-13 -0.19685060169098278 1108 14-15 -0.17858407161554624 1108 16-17 -0.982479124017587 1108 18-19 -0.09205591795584667 1108 20-21 -0.1404948951476257 1108 22-23 -0.4596506749112237 1108 24-25 -0.48802008236665984 1108 26-27 -0.3526539992671047 1108 28-29 0.17155561632559824 1108 30-31 0.5682778892630154 1108 32-33 0.7005066670666764 1108 34-35 -0.15474720317414636 1108 36-37 -0.2940611624766163 1108 38-39 0.2271200038758039 1108 40-41 0.20834565813391492 1108 42-43 0.019938661769913324 1108 44-45 0.0587199098595832 1108 46-47 0.6782253249290449 1108 48-49 0.26751439524911547 1108 50-51 0.3926937241129238 1108 52-53 -0.31223612109593546 1108 54-55 0.3786649312818966 1108 56-57 0.11262442923082716 1108 58-59 -1.0601724900925404 1108 60-61 0.9122518143811682 1108 62-63 -0.3294798747784178 1108 64-65 -0.20321511090259037 1108 66-67 -0.19090214368620906 1108 68-69 0.7665102884935209 1108 70-71 1.1707864635491063 1108 72-73 0.3842382204937991 1108 74-75 0.44732859047345386 1108 76 0.25880181917984757 1109 1 -0.06606818747308907 1109 2 -0.6049398223055569 1109 3 0.13530437670880957 1109 4 -0.2265810121845213 1109 5 0.010745226568133148 1109 6 0.2699525086874601 1109 7 -0.6791852675122065 1109 8 -0.4746201248864672 1109 9 0.32929223327474233 1109 10-11 1.1121374401018755 1109 12-13 -0.34694001296548294 1109 14-15 0.11701133802698394 1109 16-17 -0.3458968916871612 1109 18-19 -0.49816539902322887 1109 20-21 0.24602223188582428 1109 22-23 0.6041785582351835 1109 24-25 0.37982484549666395 1109 26-27 0.3579486153616216 1109 28-29 0.09096393938676073 1109 30-31 -0.10129102686400415 1109 32-33 0.24971759086744072 1109 34-35 -0.17771635194408475 1109 36-37 0.23571366000294347 1109 38-39 0.3372174014000606 1109 40-41 -0.26433615925874676 1109 42-43 0.3828008642387104 1109 44-45 0.12645546082400472 1109 46-47 -0.49376550824514 1109 48-49 -0.23979475942370954 1109 50-51 0.2792455739714299 1109 52-53 0.5488899401204108 1109 54-55 -0.08222848700662055 1109 56-57 -0.21665212070927709 1109 58-59 0.0766875248382739 1109 60-61 0.07571819400581248 1109 62-63 -0.25547685389264174 1109 64-65 -0.24649711232622806 1109 66-67 -0.28824758371121106 1109 68-69 0.6023407716616305 1109 70-71 -0.2412606206016541 1109 72-73 -0.4880685903883375 1109 74-75 1.049578629536633 1109 76 -0.342302366656714 1110 1 -0.49809459058952044 1110 2 -0.4834205610156985 1110 3 -0.3012307553067721 1110 4 -0.6857679981894265 1110 5 -0.3645613669970089 1110 6 0.4614472424921061 1110 7 -0.1884191442976615 1110 8 1.035876195990749 1110 9 0.054367979920250775 1110 10-11 -0.4226699469995552 1110 12-13 -0.668493540594973 1110 14-15 -0.24792517907011558 1110 16-17 -0.19414209439961638 1110 18-19 0.5975098282167011 1110 20-21 -0.43920536874900407 1110 22-23 0.10887665374809785 1110 24-25 0.47261448624114166 1110 26-27 -0.4873388611929883 1110 28-29 0.14708859672336416 1110 30-31 -0.16177111631718333 1110 32-33 0.7982495487378785 1110 34-35 0.4607557300065679 1110 36-37 -0.3349110694733213 1110 38-39 -0.1040879657908178 1110 40-41 0.3791676641433597 1110 42-43 -0.06838763669910009 1110 44-45 1.0914767420244118 1110 46-47 0.09535402717543207 1110 48-49 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2116 34-35 -1.3137800790119982 2116 36-37 -0.5853255257067467 2116 38-39 -0.5256728464274261 2116 40-41 -0.25088870190438683 2116 42-43 0.17954008213378359 2116 44-45 1.5244177234567715 2116 46-47 -0.31935087998637357 2116 48-49 0.47268914788237737 2116 50-51 -0.06948310320913009 2116 52-53 0.37935340970521736 2116 54-55 -0.16476389093323718 2116 56-57 -0.21671246831146718 2116 58-59 -0.8620596589402894 2116 60-61 -1.6964997815332907 2116 62-63 0.42812066783249847 2116 64-65 0.8824455976834287 2116 66-67 -0.08456436839096426 2116 68-69 0.233325242212004 2116 70-71 -2.000644672741984 2116 72-73 0.687402120908736 2116 74-75 -0.8244356929609538 2116 76 -0.4188633579666572 2117 1 -0.3697174131988348 2117 2 0.07816848981970992 2117 3 0.22040984192038593 2117 4 0.010597092461125612 2117 5 -1.2272524441388484 2117 6 -1.2608243536685748 2117 7 -0.7788919491323618 2117 8 -1.349744138172067 2117 9 -0.15981616512063113 2117 10-11 0.03858138609678008 2117 12-13 0.04553507653976396 2117 14-15 -0.3707922249286284 2117 16-17 -0.47931361946182705 2117 18-19 -0.33001416751738333 2117 20-21 0.023801207859598605 2117 22-23 0.21430235584550417 2117 24-25 0.37667598641888844 2117 26-27 -0.004202529874106631 2117 28-29 -0.7930998142258012 2117 30-31 -0.294525293352379 2117 32-33 0.4357806543191032 2117 34-35 0.17769494081353798 2117 36-37 0.08599188111323741 2117 38-39 0.034398525499618415 2117 40-41 -1.2417441737521244 2117 42-43 -0.6230223683023723 2117 44-45 -0.04856502832752341 2117 46-47 0.2053566933681168 2117 48-49 -0.02914471413190256 2117 50-51 0.40077859385668546 2117 52-53 0.6311377078812654 2117 54-55 -0.9956025110759832 2117 56-57 -1.3481062034423132 2117 58-59 -0.4613360269022273 2117 60-61 0.28008130490604444 2117 62-63 -0.5001604582579091 2117 64-65 0.26279849221158 2117 66-67 -0.21276123595639262 2117 68-69 0.2043551787702924 2117 70-71 0.6062893082255023 2117 72-73 1.441912430187081 2117 74-75 -0.39783973737015543 2117 76 0.12167986170027234 2118 1 1.1402762749980937 2118 2 0.8675646606591769 2118 3 1.465570792898717 2118 4 1.3595346056107154 2118 5 0.7709066132985569 2118 6 0.9334739842232835 2118 7 0.34113750594863745 2118 8 -4.215355331102845 2118 9 -0.5895447575885484 2118 10-11 -0.03271568943445402 2118 12-13 0.8153646578568186 2118 14-15 0.10490733324515844 2118 16-17 0.6653318675989794 2118 18-19 -0.2660545630570397 2118 20-21 -1.7731691266659624 2118 22-23 -0.02857056651932055 2118 24-25 0.6259396093938534 2118 26-27 0.5466600832123589 2118 28-29 -0.294677764993736 2118 30-31 -0.14782847029326263 2118 32-33 0.7024543341002953 2118 34-35 1.1589436258545618 2118 36-37 0.05601081652245199 2118 38-39 0.48119528544070533 2118 40-41 -0.47964655119794486 2118 42-43 0.8007626096063092 2118 44-45 0.27554605940653687 2118 46-47 -0.23060480463315258 2118 48-49 -0.30036815196064026 2118 50-51 0.8808701150012297 2118 52-53 -0.553535130326182 2118 54-55 0.7105304575753948 2118 56-57 2.1295394940119223 2118 58-59 1.6309740930220364 2118 60-61 -0.6602458568865082 2118 62-63 0.5516489252893244 2118 64-65 0.06082079391576656 2118 66-67 -1.818326142331312 2118 68-69 1.3465022437818561 2118 70-71 -0.02326044982204678 2118 72-73 -0.6301999199075929 2118 74-75 0.5575305550767204 2118 76 0.09585406746505143 2119 1 0.41567495906188157 2119 2 0.8460970572243625 2119 3 -0.23195903870605505 2119 4 0.09445152319742078 2119 5 0.652444474344616 2119 6 1.1902488253072576 2119 7 -0.8985190123964024 2119 8 0.49522234258958164 2119 9 0.19126264734063625 2119 10-11 0.2208668911387548 2119 12-13 0.0940253024425246 2119 14-15 -0.4093393490343189 2119 16-17 -0.3532362153719113 2119 18-19 -0.505962001442235 2119 20-21 -0.3566139800171406 2119 22-23 0.17641716627871062 2119 24-25 0.219811584413371 2119 26-27 0.8123007657148662 2119 28-29 0.7066325952159218 2119 30-31 0.42192730086766517 2119 32-33 0.2036810542965739 2119 34-35 0.5080988617194038 2119 36-37 -0.29204315916618384 2119 38-39 0.1920656991799703 2119 40-41 0.18016163437135901 2119 42-43 0.3232060131972574 2119 44-45 -1.0015254249248997 2119 46-47 0.14779655591107144 2119 48-49 -0.476527960146214 2119 50-51 0.833627447442403 2119 52-53 -0.8670736604923448 2119 54-55 -1.4325497253174326 2119 56-57 0.7371284312261608 2119 58-59 1.1096458896095207 2119 60-61 0.9231014164490539 2119 62-63 0.5962848124310689 2119 64-65 0.5837381575825447 2119 66-67 0.9801293054215634 2119 68-69 -0.333811685068369 2119 70-71 -0.23576184382226728 2119 72-73 -0.7576115402934498 2119 74-75 -0.12810845462551868 2119 76 0.6891126823864298 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 3.0 12 29.0 13 231.0 14 775.0 15 1236.0 16 1560.0 17 1861.0 18 1954.0 19 1846.0 20 1614.0 21 1382.0 22 1078.0 23 905.0 24 863.0 25 850.0 26 882.0 27 1065.0 28 1257.0 29 1599.0 30 1853.0 31 2418.0 32 3094.0 33 4044.0 34 5939.0 35 8935.0 36 15664.0 37 20673.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.16454780984515 26.060131349038983 9.360727842381152 27.41459299873471 2 18.12201743789693 27.519106337682842 31.432467019889724 22.926409204530504 3 16.362679551733624 25.13066462546794 29.261700015548193 29.244955807250246 4 14.148856011768787 20.883615792180453 35.84695793615673 29.120570259894034 5 11.084665893243711 38.60257621604813 33.58290177129803 16.729856119410126 6 22.691990288359186 41.69786272141226 18.046668500556144 17.563478489672413 7 22.782887419119493 33.35565894439727 22.98381791869491 20.87763571778833 8 20.37411345397137 35.30755522598702 21.957637153006182 22.36069416703544 9 21.572520362153305 20.338233007618616 21.244812285464832 36.84443434476325 10-11 21.67178959706259 26.327875518771453 28.12967193311885 23.870662951047112 12-13 21.18381552666515 24.60382007152169 26.832593797466842 27.37977060434632 14-15 19.724079367547333 26.135317123345015 24.440564040616668 29.700039468490992 16-17 21.473251127244023 28.444223846144645 25.20840559256557 24.87411943404576 18-19 20.4327181830142 27.584887155996217 25.60249249500664 26.379902165982944 20-21 19.893315472844485 28.151798208369712 25.83810742605638 26.116778892729425 22-23 21.647271292054874 27.467677697910563 25.470930858379877 25.414120151654686 24-25 20.667735106624725 29.18515506332899 25.246080061235958 24.901029768810325 26-27 19.771919962684336 29.463228522562822 26.25133056655225 24.513520948200597 28-29 20.817236966427863 27.833060243269426 27.689538457858415 23.660164332444296 30-31 20.646804846252287 27.608807453564722 27.615385535396058 24.129002164786932 32-33 19.141620121754315 29.4955209242803 27.290069488464436 24.07278946550095 34-35 20.4901268971786 28.292927964023875 26.82840774539235 24.388537393405173 36-37 20.656372965279687 27.68834244297999 26.943823181160376 24.711461410579947 38-39 20.31610673236775 27.128607479877047 27.88747892023777 24.66780686751743 40-41 20.054179473992654 26.514453839805768 28.128475918240426 25.302890767961156 42-43 18.93171951059071 26.675915848393156 28.577579505089044 25.814785135927092 44-45 18.941287629618113 26.83498582722369 28.893925440432476 25.329801102725717 46-47 18.984942172680626 27.136381576586814 28.652928442429825 25.225747808302735 48-49 18.602815419023813 28.161366327397115 28.095585509083733 25.14023274449534 50-51 18.444343447632487 27.772063484469744 27.569936970015906 26.21365609788186 52-53 18.113645333747954 27.567544940259058 27.90960519548863 26.40920453050436 54-55 17.7440767363146 27.74216311250912 27.430003229240175 27.083756921936107 56-57 18.36660248053486 27.482627883890874 27.666216167729125 26.48455346784514 58-59 18.50534020643217 27.280501369437037 27.22309265527263 26.991065768858164 60-61 18.019758165791583 26.669337766561817 27.406680939110885 27.904223128535723 62-63 18.27570534977455 26.953391300187775 27.036514334238316 27.734389015799355 64-65 18.843214409587254 27.277511332240973 26.15983542835273 27.719438829819044 66-67 18.639891880254993 28.04714690650752 25.664685268684746 27.648275944552754 68-69 19.113513772111325 28.143426104220737 25.633588881845693 27.10947124182225 70-71 19.724079367547333 27.942495604645323 25.56362201145782 26.769803016349524 72-73 20.26168805539941 27.74156510506991 25.357309444929495 26.639437394601188 74-75 20.21922952721532 28.20143282582435 24.904617813445597 26.674719833514725 76 20.199495281721305 27.52149836743969 25.68561552905718 26.59339082178182 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 1.0 14 5.5 15 12.5 16 26.5 17 37.0 18 38.5 19 58.5 20 77.0 21 77.0 22 88.5 23 133.5 24 209.0 25 251.0 26 290.5 27 394.0 28 528.5 29 599.0 30 698.5 31 916.5 32 1278.0 33 1521.0 34 1665.5 35 2077.0 36 2508.0 37 2672.0 38 2904.5 39 3543.5 40 4265.0 41 4918.5 42 5257.0 43 5316.0 44 5564.0 45 5762.5 46 5772.0 47 5787.5 48 5659.0 49 5274.5 50 5034.0 51 4722.0 52 4019.0 53 3336.5 54 3045.0 55 2782.5 56 2281.5 57 1787.0 58 1531.0 59 1351.5 60 1000.5 61 709.5 62 590.0 63 507.5 64 359.5 65 241.0 66 149.5 67 111.0 68 96.0 69 72.5 70 48.0 71 32.0 72 27.0 73 20.5 74 15.5 75 12.0 76 11.0 77 9.5 78 7.0 79 5.0 80 6.0 81 6.0 82 4.5 83 4.0 84 5.5 85 5.0 86 3.0 87 3.0 88 2.0 89 1.0 90 2.0 91 3.0 92 3.0 93 2.5 94 2.5 95 2.0 96 1.0 97 1.0 98 2.0 99 3.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7487053138941049 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 83611.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.0665343076868 #Duplication Level Percentage of deduplicated Percentage of total 1 80.05171645118145 53.68791187762376 2 10.18992420864913 13.668058030641902 3 4.042799821667409 8.13409718816902 4 2.0615247436469013 5.530372797837605 5 1.1288452964779312 3.7853870902154023 6 0.6705305394560857 2.6982095657269975 7 0.45831475702184576 2.1516307662867327 8 0.4101649576460098 2.200667376302161 9 0.24431564868479713 1.474686345098133 >10 0.7418635755684352 6.668978962098289 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0023920297568501754 0.0 2 0.0 0.0 0.0 0.008372104148975613 0.0 3 0.0 0.0 0.0 0.008372104148975613 0.0 4 0.0 0.0 0.0 0.008372104148975613 0.0 5 0.0 0.0 0.0 0.008372104148975613 0.0 6 0.0 0.0 0.0 0.009568119027400701 0.0 7 0.0 0.0 0.0 0.009568119027400701 0.0 8 0.0 0.0 0.0 0.009568119027400701 0.0 9 0.0 0.0 0.0 0.010764133905825788 0.0 10 0.0 0.0 0.0 0.010764133905825788 0.0 11 0.0 0.0 0.0 0.010764133905825788 0.0 12 0.0 0.0 0.0 0.013156163662675963 0.0 13 0.0 0.0 0.0 0.014352178541101051 0.0 14 0.0 0.0 0.0 0.016744208297951226 0.0 15 0.0 0.0 0.0 0.019136238054801403 0.0 16 0.0 0.0 0.0 0.020332252933226488 0.0 17 0.0 0.0 0.0 0.022724282690076664 0.0 18 0.0 0.0 0.0 0.028704357082202103 0.0 19 0.0 0.0 0.0 0.03588044635275263 0.0 20 0.0 0.0 0.0 0.04186052074487807 0.0 21 0.0 0.0 0.0 0.044252550501728244 0.0 22 0.0 0.0 0.0 0.044252550501728244 0.0 23 0.0 0.0 0.0 0.04903661001542859 0.0 24 0.0 0.0 0.0 0.05501668440755403 0.0 25 0.0 0.0 0.0 0.05860472904282929 0.0 26 0.0 0.0 0.0 0.06817284807023 0.0 27 0.0 0.0 0.0 0.07415292246235543 0.0 28 0.0 0.0 0.0 0.08372104148975613 0.0 29 0.0 0.0 0.0 0.11242539857195824 0.0 30 0.0 0.0 0.0 0.12797359199148436 0.0 31 0.0 0.0 0.0 0.16863809785793735 0.0 32 0.0 0.0 0.0 0.21408666323809067 0.0 33 0.0 0.0 0.0 0.2679073327672196 0.0 34 0.0 0.0 0.0 0.3289040915668991 0.0 35 0.0 0.0 0.0 0.42817332647618134 0.0 36 0.0 0.0 0.0 0.5824592457930177 0.0 37 0.0 0.0 0.0 0.833622370262286 0.0 38 0.0 0.0 0.0 1.1589384171939099 0.0 39 0.0 0.0 0.0 1.6206001602659936 0.0 40 0.0 0.0 0.0 2.1396706175024818 0.0 41 0.0 0.0 0.0 2.657545059860545 0.0 42 0.0 0.0 0.0 3.2017318295439594 0.0 43 0.0 0.0 0.0 3.753094688497925 0.0 44 0.0 0.0 0.0 4.266185071342288 0.0 45 0.0 0.0 0.0 4.769707335159249 0.0 46 0.0 0.0 0.0 5.311502075085814 0.0 47 0.0 0.0 0.0 5.852100800133954 0.0 48 0.0 0.0 0.0 6.42259989714272 0.0 49 0.0 0.0 0.0 6.979942830488811 0.0 50 0.0 0.0 0.0 7.555225987011278 0.0 51 0.0 0.0 0.0 8.137685232804296 0.0 52 0.0 0.0 0.0 8.727320567867864 0.0 53 0.0 0.0 0.0 9.208118548994749 0.0 54 0.0 0.0 0.0 9.735561110380214 0.0 55 0.0 0.0 0.0 10.236691344440326 0.0 56 0.0 0.0 0.0 10.72227338508091 0.0 57 0.0 0.0 0.0 11.226991663776298 0.0 58 0.0 0.0 0.0 11.74247407637751 0.0 59 0.0 0.0 0.0 12.225664087261245 0.0 60 0.0 0.0 0.0 12.744734544497733 0.0 61 0.0 0.0 0.0 13.224336510746193 0.0 62 0.0 0.0 0.0 13.743406967982681 0.0 63 0.0 0.0 0.0 14.224204949109566 0.0 64 0.0 0.0 0.0 14.687062707060075 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 15 0.002218577 69.99283 59 ACATTCG 15 0.002218577 69.99283 10 ATCCAGT 15 0.002218577 69.99283 45 AACTAGC 15 0.002218577 69.99283 38 CATCCGG 15 0.002218577 69.99283 8 AAGGCAA 15 0.002218577 69.99283 7 ATCCAAC 20 0.0067375796 52.874096 1 GATTATA 20 0.0067375796 52.874096 1 AACGTTG 20 0.006932433 52.494617 24 CGGCGGC 20 0.006932433 52.494617 25 GGCAAGC 20 0.006932433 52.494617 27 GTTAGTG 20 0.006932433 52.494617 31 CTGACAT 20 0.006932433 52.494617 4 ATCCGGC 20 0.006932433 52.494617 9 ACTAGCC 20 0.006932433 52.494617 39 AGGCAAG 20 0.006932433 52.494617 8 CATCGCG 20 0.006932433 52.494617 8 CCAACGT 20 0.006932433 52.494617 22 CGCAAAG 20 0.006932433 52.494617 12 TTTTCGC 20 0.006932433 52.494617 9 >>END_MODULE