FastQCFastQC Report
Wed 25 May 2016
SRR1780520_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780520_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83611
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT31223.7339584504431236No Hit
ATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTT29743.5569482484362105No Hit
CTTTACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC8621.0309648252024255No Hit
CTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC4200.5023262489385368No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG3520.42099723720563087No Hit
TATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCT2870.34325627010800014No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC1750.20930260372439033No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC1620.19375441030486418No Hit
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATATCGTATGCCGT1360.16265802346581193No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG1160.13873772589731015No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC1090.13036562174833455No Hit
CTTAACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC920.11003336881510806No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCT150.00221767570.0000138
TTTCGTG150.00221767570.0000124
TTCGTGC150.00221767570.0000125
AGCGCTA150.00221767570.0000139
ACAGCGC150.00221767570.0000137
CACAGCG150.00221767570.0000136
TTTCTGC150.00221767570.0000151
CTATAAC252.3781901E-456.0000043
GACTTAA200.00692962452.4999961
CTTTATA200.00692962452.4999962
TGGCTTA200.00692962452.49999639
CTGGATG200.00692962452.49999658
ATCTGAT200.00692962452.49999640
TTTACAC1450.050.6896552
CTATACA800.048.1249961
TTACACA1550.047.4193533
CTTTACA1600.045.9374961
TATATCG551.293156E-744.54545236
CGTTATA551.293156E-744.54545233
ATATCGT551.293156E-744.54545237
Produced by FastQC (version 0.11.5)