##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780520_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 83611 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.00772625611462 34.0 34.0 34.0 32.0 34.0 2 33.320878831732664 34.0 34.0 34.0 33.0 34.0 3 33.487770747868105 34.0 34.0 34.0 34.0 34.0 4 33.51249237540515 34.0 34.0 34.0 34.0 34.0 5 33.51246845510758 34.0 34.0 34.0 34.0 34.0 6 37.25367475571396 38.0 37.0 38.0 36.0 38.0 7 36.860508784729284 38.0 37.0 38.0 35.0 38.0 8 36.8958869048331 38.0 37.0 38.0 35.0 38.0 9 36.70271854181866 38.0 37.0 38.0 34.0 38.0 10-11 36.406884261640215 38.0 37.0 38.0 34.0 38.0 12-13 36.24616976235184 38.0 37.0 38.0 34.0 38.0 14-15 36.454772697372356 38.0 37.0 38.0 34.0 38.0 16-17 36.55793496071091 38.0 37.0 38.0 34.0 38.0 18-19 36.59949049766179 38.0 37.0 38.0 34.0 38.0 20-21 36.355413761347194 38.0 37.0 38.0 34.0 38.0 22-23 36.411285596392815 38.0 37.0 38.0 34.0 38.0 24-25 35.718858762603006 38.0 36.5 38.0 31.5 38.0 26-27 35.93029027281099 38.0 37.0 38.0 32.5 38.0 28-29 35.818666204207574 38.0 36.5 38.0 31.5 38.0 30-31 35.50047840595137 38.0 36.0 38.0 30.0 38.0 32-33 35.9466098958271 38.0 37.0 38.0 33.0 38.0 34-35 35.836959251773095 38.0 37.0 38.0 32.0 38.0 36-37 35.91522646541723 38.0 37.0 38.0 32.5 38.0 38-39 35.42888495532884 38.0 36.5 38.0 29.5 38.0 40-41 35.2118800157874 37.0 35.5 38.0 29.0 38.0 42-43 35.74888471612587 38.0 36.5 38.0 32.5 38.0 44-45 35.978047146906505 38.0 37.0 38.0 33.0 38.0 46-47 36.19375441030486 38.0 37.0 38.0 34.0 38.0 48-49 35.97024314982478 38.0 37.0 38.0 32.5 38.0 50-51 36.11314898757341 38.0 37.0 38.0 34.0 38.0 52-53 35.9633959646458 38.0 37.0 38.0 32.5 38.0 54-55 36.16473908935427 38.0 37.0 38.0 34.0 38.0 56-57 36.18807931970674 38.0 37.0 38.0 34.0 38.0 58-59 36.219014244537206 38.0 37.0 38.0 34.0 38.0 60-61 35.94238796330626 38.0 37.0 38.0 32.5 38.0 62-63 36.27881498845846 38.0 37.0 38.0 34.0 38.0 64-65 36.27952063723673 38.0 37.0 38.0 34.0 38.0 66-67 35.46272021623949 38.0 36.0 38.0 31.0 38.0 68-69 34.3263984403966 37.5 34.5 38.0 24.0 38.0 70-71 33.8021731590341 37.0 34.0 38.0 22.0 38.0 72-73 33.07366853643659 37.0 33.5 38.0 16.0 38.0 74-75 33.53392496202653 37.5 34.0 38.0 21.0 38.0 76 33.14986066426666 37.0 34.0 38.0 20.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28410744586530257 1101 2 0.3742202497139431 1101 3 0.09281540192171889 1101 4 0.31428570647908316 1101 5 0.2689937732700898 1101 6 -1.1705935853951885 1101 7 -0.059593783574165116 1101 8 0.27051467470571566 1101 9 0.20192912188458934 1101 10-11 0.2671106225904083 1101 12-13 0.7729242223115236 1101 14-15 0.8019098224521244 1101 16-17 0.0044042072039260916 1101 18-19 -0.4541894134285869 1101 20-21 -0.44745760749110985 1101 22-23 0.409301438152724 1101 24-25 -1.9243776196188662 1101 26-27 -0.43979910989598636 1101 28-29 0.8698917505446389 1101 30-31 1.0780675240773334 1101 32-33 0.1891990796278975 1101 34-35 0.6031354672691904 1101 36-37 0.08208541535493197 1101 38-39 0.607972982462563 1101 40-41 1.437951000255282 1101 42-43 0.5611691123437055 1101 44-45 0.17304281162098079 1101 46-47 0.7494028731576208 1101 48-49 -1.4535567403560563 1101 50-51 -1.5466850467273048 1101 52-53 -2.1884152857078334 1101 54-55 -1.3313407441384086 1101 56-57 -1.9063463708504784 1101 58-59 -1.453354123097185 1101 60-61 -2.3650971389276805 1101 62-63 -1.5902366497018932 1101 64-65 -1.2606730223900158 1101 66-67 -1.968999533078481 1101 68-69 -1.1213851713379057 1101 70-71 -0.44450973976602626 1101 72-73 -1.5010113904941136 1101 74-75 -0.3707889189065483 1101 76 -0.10520570101717652 1102 1 -0.660768617767701 1102 2 0.15474189233325575 1102 3 0.2587421499794331 1102 4 0.3949368348549669 1102 5 0.36351837556384226 1102 6 0.36834463140096574 1102 7 -0.7207110606736862 1102 8 0.5305960657526967 1102 9 0.6510597175190682 1102 10-11 0.47895856931626213 1102 12-13 0.5688454209796703 1102 14-15 0.10102192012137579 1102 16-17 0.2848796049101807 1102 18-19 -0.3865331056224406 1102 20-21 0.3767654779694922 1102 22-23 0.746195629791643 1102 24-25 -4.378641400602959 1102 26-27 -1.1116452068253224 1102 28-29 0.3513937853208233 1102 30-31 1.4953816195268388 1102 32-33 0.3043489131461996 1102 34-35 0.3403718712647432 1102 36-37 -2.1120044847560564 1102 38-39 -0.18591344003095145 1102 40-41 0.9215340561191283 1102 42-43 0.9041676325064856 1102 44-45 1.020110884133011 1102 46-47 1.131293365203497 1102 48-49 -3.170769096996821 1102 50-51 -0.48661290466293394 1102 52-53 -0.09856141963310705 1102 54-55 0.5571627704749815 1102 56-57 1.0856995041032746 1102 58-59 0.2944723659890087 1102 60-61 -6.061129325387171 1102 62-63 -2.525123812114032 1102 64-65 0.24557004087302658 1102 66-67 1.3255805631109325 1102 68-69 -0.48667373959539617 1102 70-71 -0.7536569835692077 1102 72-73 -5.366346203664662 1102 74-75 -2.3862347198684404 1102 76 -0.24921236028465188 1103 1 0.717783325855855 1103 2 0.5625618514148698 1103 3 -0.028198063705495713 1103 4 0.2748337811306314 1103 5 0.347188906745167 1103 6 0.1539398424337719 1103 7 0.8133686544130754 1103 8 0.5321494490973961 1103 9 0.4382272555959048 1103 10-11 0.036452696769472936 1103 12-13 -0.761921041095043 1103 14-15 -0.32232240055756733 1103 16-17 0.03564682473287206 1103 18-19 0.5959818599000286 1103 20-21 -0.1665398186888396 1103 22-23 0.5852404884716478 1103 24-25 -3.2342051650948846 1103 26-27 -0.5353873451901094 1103 28-29 -0.5726135648345192 1103 30-31 0.8914091257782601 1103 32-33 0.9369548084254404 1103 34-35 0.2749403337941132 1103 36-37 -1.2533150098754007 1103 38-39 -0.4699268521700759 1103 40-41 -0.335200428991115 1103 42-43 -0.4711070511337283 1103 44-45 0.8470033596956412 1103 46-47 0.797211755746801 1103 48-49 -2.5036098941476865 1103 50-51 -0.7843415551137909 1103 52-53 -0.024406308623028394 1103 54-55 -0.7169715024658316 1103 56-57 0.22222674514290475 1103 58-59 -0.5779702346020343 1103 60-61 -4.424658915445519 1103 62-63 -1.1554091042258747 1103 64-65 0.028513134000256457 1103 66-67 -0.8202575061472288 1103 68-69 -0.4579077321694669 1103 70-71 0.10781721511230558 1103 72-73 -4.011199200557897 1103 74-75 -2.154600636364613 1103 76 -2.8134504446977786 1104 1 -0.013372703219125981 1104 2 0.04668014276207799 1104 3 0.13450232462468392 1104 4 -0.21788087916343812 1104 5 -0.5215588114001406 1104 6 -0.24720191467731212 1104 7 0.36088101791667526 1104 8 0.29077071100453367 1104 9 0.8332287491735215 1104 10-11 -0.7020799461990919 1104 12-13 0.05646821842235994 1104 14-15 0.4442895015965078 1104 16-17 0.10900637865102425 1104 18-19 0.5189713384754882 1104 20-21 -0.2599879915455574 1104 22-23 -0.057114053663831044 1104 24-25 0.4988925148812129 1104 26-27 0.891609899988957 1104 28-29 1.1513898869893069 1104 30-31 0.5284199791089392 1104 32-33 0.26856222984666545 1104 34-35 0.22151174339946778 1104 36-37 1.0724840541492924 1104 38-39 1.0622348524998415 1104 40-41 1.6521788401515707 1104 42-43 0.08812989665743487 1104 44-45 0.4690037579830033 1104 46-47 0.9294182677452056 1104 48-49 -0.31660567893974445 1104 50-51 0.5199168006232071 1104 52-53 0.6942617520494139 1104 54-55 -0.0840347969661508 1104 56-57 -0.08613894903878361 1104 58-59 -1.0455596003294048 1104 60-61 0.2556490934495699 1104 62-63 0.3015758604261194 1104 64-65 0.6511524516012415 1104 66-67 -0.21841210802581656 1104 68-69 1.1886537202517857 1104 70-71 1.114637886226518 1104 72-73 0.8248474654449538 1104 74-75 0.30047586799995685 1104 76 0.2594029523615333 1105 1 0.8091210086026521 1105 2 0.18897263288064892 1105 3 0.343629166162593 1105 4 0.012805430574260868 1105 5 -0.07689477977958603 1105 6 0.22638773677795143 1105 7 0.9257390847510294 1105 8 -0.2444430870551173 1105 9 0.9586329892022647 1105 10-11 0.5152215270312297 1105 12-13 -0.1996402975675764 1105 14-15 0.26938831582575773 1105 16-17 0.12598445985835127 1105 18-19 0.5869734944223026 1105 20-21 0.8116972402187272 1105 22-23 0.6830943545287624 1105 24-25 0.7058634095991394 1105 26-27 -0.056467714096562815 1105 28-29 0.9622055535361937 1105 30-31 1.1087289981531327 1105 32-33 0.8192269801161558 1105 34-35 -0.4465622774413518 1105 36-37 0.8514995051015077 1105 38-39 0.15026934764895827 1105 40-41 0.5130304391454601 1105 42-43 0.3934658650368874 1105 44-45 1.111206416984082 1105 46-47 1.0947973550610541 1105 48-49 -0.5612158118898023 1105 50-51 0.6171140697572355 1105 52-53 0.5808050506480527 1105 54-55 1.0615814444998932 1105 56-57 0.5903791968469534 1105 58-59 0.47743716575036643 1105 60-61 0.718459010804942 1105 62-63 -0.6330270860345308 1105 64-65 0.33979779834935897 1105 66-67 0.5261081938067349 1105 68-69 0.47440650501642523 1105 70-71 1.023908457552423 1105 72-73 0.48609432135584285 1105 74-75 -0.17832398827025742 1105 76 1.119625733532935 1106 1 -1.097213503567101 1106 2 0.17427184930114947 1106 3 0.12145317281954959 1106 4 0.026102011948644588 1106 5 -0.046279704542087075 1106 6 0.13977793163267194 1106 7 -1.066043731974581 1106 8 0.4784610111350247 1106 9 -0.6987416727490583 1106 10-11 0.5181426143344723 1106 12-13 1.219002653684079 1106 14-15 -0.06328625597925708 1106 16-17 0.3832741762441785 1106 18-19 0.4647992346622303 1106 20-21 0.14335766598670574 1106 22-23 0.41923435868935854 1106 24-25 -0.26390290341660005 1106 26-27 0.9997003741081372 1106 28-29 0.5989939177273058 1106 30-31 0.3781191236645398 1106 32-33 -0.16283910406663438 1106 34-35 0.9162159626252233 1106 36-37 1.301641658904984 1106 38-39 0.25528980392800094 1106 40-41 0.7254896999456832 1106 42-43 0.7635684931486679 1106 44-45 1.318811645470312 1106 46-47 0.9440623158820785 1106 48-49 1.254131598137242 1106 50-51 0.38623548990840106 1106 52-53 0.8692472529918547 1106 54-55 0.5637830948708782 1106 56-57 0.7227815961257846 1106 58-59 0.7269864341783503 1106 60-61 1.015707814632691 1106 62-63 0.7997272305870595 1106 64-65 -0.6226441266211751 1106 66-67 0.8527238931341117 1106 68-69 1.7127221558034762 1106 70-71 0.434360229274219 1106 72-73 0.6663002710125951 1106 74-75 -0.27571667948446077 1106 76 0.8702844950612558 1107 1 0.7902585549575889 1107 2 -0.11618402010208939 1107 3 0.2582139937909034 1107 4 0.0963282532275187 1107 5 0.19571040586907884 1107 6 0.21503363846733237 1107 7 0.765392967883173 1107 8 0.15108852586779875 1107 9 -0.5418629895545877 1107 10-11 0.27707541931990676 1107 12-13 0.13438231906373943 1107 14-15 0.23851554582254408 1107 16-17 0.7176489645663295 1107 18-19 0.667612009843431 1107 20-21 0.925100875361494 1107 22-23 0.2479531150396994 1107 24-25 0.3810202151907305 1107 26-27 -0.04053535033811073 1107 28-29 0.6678230852406841 1107 30-31 0.6721227137207606 1107 32-33 1.0963646485581648 1107 34-35 0.29192720017857 1107 36-37 1.224658660869359 1107 38-39 0.4838341377354922 1107 40-41 0.8501510153964773 1107 42-43 0.9500990086265446 1107 44-45 0.9786961540390351 1107 46-47 0.8984584413308383 1107 48-49 0.8808080866558328 1107 50-51 1.1661244013762655 1107 52-53 0.7711388061991897 1107 54-55 0.517332508946609 1107 56-57 0.8249542575089706 1107 58-59 0.8459493820886692 1107 60-61 1.1835344758803998 1107 62-63 0.6038828900058775 1107 64-65 0.6632141000265221 1107 66-67 -0.6442872157191033 1107 68-69 0.6663731752439688 1107 70-71 -0.34612984726848595 1107 72-73 0.17813729600751316 1107 74-75 -1.192264806558903 1107 76 0.4941583689351319 1108 1 -0.35780533584489405 1108 2 0.35109357165683264 1108 3 0.22846343706938654 1108 4 0.4183455876929827 1108 5 -0.011373975489163968 1108 6 -0.2678802178905144 1108 7 -0.2856331619811314 1108 8 -0.16512929242470875 1108 9 -0.3141598863328099 1108 10-11 0.23040693696677295 1108 12-13 1.0927924185526763 1108 14-15 0.4892725268699891 1108 16-17 -0.16647285731482242 1108 18-19 -0.09257319017579846 1108 20-21 0.6613110036878069 1108 22-23 -0.09116638383083853 1108 24-25 0.7463977444686734 1108 26-27 -0.7566874835026951 1108 28-29 -0.6934371079697144 1108 30-31 0.21313586676711083 1108 32-33 0.8999020330084164 1108 34-35 0.3395889698320431 1108 36-37 1.1614727719573281 1108 38-39 1.0370471017368317 1108 40-41 0.9315845532615441 1108 42-43 -0.2676792319732684 1108 44-45 0.42921602779542667 1108 46-47 0.8093349372695684 1108 48-49 0.9658863480826412 1108 50-51 0.6549258881030582 1108 52-53 -0.3760119011174936 1108 54-55 -0.20430673551420853 1108 56-57 0.27913487559000316 1108 58-59 0.09407895801907529 1108 60-61 0.9408279281946008 1108 62-63 0.9117058285342097 1108 64-65 0.4932017640390782 1108 66-67 0.9545929329535738 1108 68-69 1.223623371498313 1108 70-71 -0.4836440656955716 1108 72-73 -0.28767439594525257 1108 74-75 -0.6919283705378163 1108 76 -0.4779174413149505 1109 1 -0.8754011274384936 1109 2 0.1056379213851244 1109 3 0.26393280285699205 1109 4 0.17120588040639717 1109 5 0.025987784263612923 1109 6 -0.14657602858166996 1109 7 -0.15974555976581684 1109 8 0.2965627508399322 1109 9 0.5463585254451715 1109 10-11 0.5183720058047783 1109 12-13 0.5094489577287007 1109 14-15 0.7832967286790407 1109 16-17 0.7400880204781899 1109 18-19 0.507897304103615 1109 20-21 0.6546529019846474 1109 22-23 0.04071090570543845 1109 24-25 -0.4260184433123655 1109 26-27 -0.08838595048996467 1109 28-29 -1.2285679262130884 1109 30-31 0.23783247608324132 1109 32-33 0.6616947724656939 1109 34-35 0.15192946185447198 1109 36-37 1.084362058229658 1109 38-39 0.6282986859657242 1109 40-41 0.31540198064743663 1109 42-43 0.9406403569084816 1109 44-45 0.9406919214741336 1109 46-47 0.8213837575615841 1109 48-49 0.8057446789564437 1109 50-51 0.1291702326229256 1109 52-53 -0.602005407949207 1109 54-55 -0.5441043014441718 1109 56-57 0.03977308111440436 1109 58-59 -0.028996095367268993 1109 60-61 0.8723197943728351 1109 62-63 0.4715506175062387 1109 64-65 0.7235443047081915 1109 66-67 0.4534818025520053 1109 68-69 -0.2620447824626879 1109 70-71 0.5307138224622037 1109 72-73 -0.30633791440912006 1109 74-75 -0.07403569879004834 1109 76 -0.01709346867653494 1110 1 -0.7551082404092071 1110 2 -0.3040251863915273 1110 3 -0.05753956765231294 1110 4 -0.03983870190495509 1110 5 0.3169991823302709 1110 6 0.059545021771818085 1110 7 0.4509707870816797 1110 8 0.804912916761154 1110 9 0.9745795613707244 1110 10-11 -0.6921946512432697 1110 12-13 0.3583704697760268 1110 14-15 0.6206217899977631 1110 16-17 0.4822608671092681 1110 18-19 -0.31862484277273495 1110 20-21 0.7823851916975357 1110 22-23 -0.3548128282434888 1110 24-25 -1.0076264363869498 1110 26-27 1.0860207007869107 1110 28-29 0.8624542928029229 1110 30-31 0.2187876052132438 1110 32-33 0.9648075988976714 1110 34-35 1.3683484490136095 1110 36-37 1.3848237520689324 1110 38-39 0.9859310622462019 1110 40-41 -1.025115260731873 1110 42-43 0.03209541389881565 1110 44-45 0.8983639745689231 1110 46-47 0.8436388434077884 1110 48-49 1.5640227052152795 1110 50-51 -0.29529052340073036 1110 52-53 0.7638328549277276 1110 54-55 -0.1890148482628149 1110 56-57 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-0.6560955745026931 2116 46-47 -0.6809798568574621 2116 48-49 0.2614696548174038 2116 50-51 -1.0220809743291142 2116 52-53 -0.4106532193428336 2116 54-55 -0.2691706838065855 2116 56-57 -0.23972683842212916 2116 58-59 -2.2080675469282625 2116 60-61 -1.2378346954887647 2116 62-63 0.04871508483806508 2116 64-65 -1.9005203074578816 2116 66-67 -1.059261599443488 2116 68-69 -1.135666845546048 2116 70-71 0.7044344532958178 2116 72-73 0.1926160604862801 2116 74-75 -0.0877949520890482 2116 76 -0.7194590946823283 2117 1 0.7320051616526513 2117 2 0.4201571811875695 2117 3 0.38678144194597763 2117 4 -0.22887176269414766 2117 5 0.09606068250801769 2117 6 0.09193926279563414 2117 7 0.4800767238040393 2117 8 0.6162797848734911 2117 9 -0.5069348360697319 2117 10-11 0.07373585000095773 2117 12-13 -1.2904475181372348 2117 14-15 0.10080309109978458 2117 16-17 -0.8278173412092471 2117 18-19 -0.6323024112050106 2117 20-21 0.2146861159776634 2117 22-23 -0.018028256611778204 2117 24-25 -1.0622389497179157 2117 26-27 -1.5027412970801421 2117 28-29 -1.0880878330413708 2117 30-31 -0.14532544135625614 2117 32-33 -1.9358767238893932 2117 34-35 0.344797080368032 2117 36-37 -1.0593639478955197 2117 38-39 0.4134106214356805 2117 40-41 -0.07377327708452697 2117 42-43 -0.24434433014668855 2117 44-45 -0.12256563794441178 2117 46-47 -0.00678774742051047 2117 48-49 0.07704261278727387 2117 50-51 7.6795509198746E-4 2117 52-53 1.073511134344713 2117 54-55 -0.16035327698659785 2117 56-57 0.4086425113003216 2117 58-59 -1.0103078324159753 2117 60-61 -0.3956459563928121 2117 62-63 -0.025828927850270134 2117 64-65 -1.0842782376720024 2117 66-67 -1.015719767564029 2117 68-69 -0.1514033641820518 2117 70-71 -0.8360096386946765 2117 72-73 -0.11923960961681246 2117 74-75 -0.3574222320256055 2117 76 0.07732916860077665 2118 1 -0.03572048530716643 2118 2 0.17283969749306038 2118 3 -1.010125774548868 2118 4 0.17603040435823658 2118 5 0.23307940752379608 2118 6 0.1138201801110128 2118 7 0.7473289855334713 2118 8 0.4330797007161209 2118 9 -0.9012121346180137 2118 10-11 -1.1533575646845122 2118 12-13 -1.309908910941779 2118 14-15 0.44011510456495984 2118 16-17 -2.793865100519163 2118 18-19 -1.0018584810556277 2118 20-21 -0.9614131897239986 2118 22-23 0.5500077206657323 2118 24-25 0.20982606185372532 2118 26-27 -3.134026800972414 2118 28-29 -0.7708145319683553 2118 30-31 -1.0875987424292717 2118 32-33 -0.27718747499395135 2118 34-35 -0.1381610513382583 2118 36-37 -3.3125724477903233 2118 38-39 -0.040986601370967435 2118 40-41 -1.187149039760726 2118 42-43 0.15700744767784158 2118 44-45 -2.3911328586471186 2118 46-47 -0.3626419447695568 2118 48-49 0.087509686214581 2118 50-51 -0.8335998126336648 2118 52-53 -2.2549919163600975 2118 54-55 -1.8331914844345292 2118 56-57 -0.2370286437596434 2118 58-59 -0.703554203129201 2118 60-61 -0.9311235494919075 2118 62-63 -1.4795423719908172 2118 64-65 -0.21385429489901497 2118 66-67 0.6996179139003118 2118 68-69 -1.0732159036308246 2118 70-71 -0.31930965973401726 2118 72-73 1.0803711625270935 2118 74-75 0.9239789882563159 2118 76 0.1372912977823475 2119 1 0.6287715410115595 2119 2 -0.4481037618578938 2119 3 0.08003815987736118 2119 4 -0.406382888827892 2119 5 -0.06626820037182313 2119 6 -0.5642772883845808 2119 7 -0.6925896048535023 2119 8 0.326605963680322 2119 9 -0.6625035364246372 2119 10-11 -0.6745881880814011 2119 12-13 -0.7359153791028135 2119 14-15 -0.3911551084382623 2119 16-17 -0.5988584093180904 2119 18-19 -0.6288519013745812 2119 20-21 -0.976105952074839 2119 22-23 -0.1073330845088023 2119 24-25 0.4402961187289307 2119 26-27 0.3635197586350287 2119 28-29 -0.3901130710561276 2119 30-31 0.9053336764399162 2119 32-33 0.025505733436958167 2119 34-35 0.3414029053919805 2119 36-37 -0.4284417463294119 2119 38-39 -2.2664243174410004 2119 40-41 -0.35672916689354395 2119 42-43 0.19605339392519738 2119 44-45 0.3447319790912502 2119 46-47 -0.2998979947305216 2119 48-49 -0.9463645192052965 2119 50-51 0.8816338218037174 2119 52-53 -0.17306652325203942 2119 54-55 0.08456250240610785 2119 56-57 -1.3739612856688694 2119 58-59 -0.2175778453729862 2119 60-61 0.41989964667180146 2119 62-63 -0.7752655793065273 2119 64-65 -0.004043321329255889 2119 66-67 0.44802875364604233 2119 68-69 0.5970333436090556 2119 70-71 -0.3770073289656466 2119 72-73 0.34698764518573455 2119 74-75 -0.311523409424062 2119 76 -0.2048521169606019 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 3.0 22 6.0 23 27.0 24 46.0 25 88.0 26 166.0 27 345.0 28 608.0 29 1013.0 30 1618.0 31 2539.0 32 3855.0 33 5713.0 34 8628.0 35 11879.0 36 17637.0 37 29439.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.99380464292976 21.777038906363995 10.533303034289746 29.6958534164165 2 18.485605960938152 19.64095633349679 40.24231261436893 21.631125091196136 3 16.215569721687338 25.769336570546937 35.785961177357045 22.22913253040868 4 11.6180885290213 22.64295367834376 36.962839817727335 28.776117974907606 5 10.721077370202485 38.211479350803124 37.76895384578584 13.298489433208548 6 22.48507971439165 40.250684718517896 18.502350169236106 18.76188539785435 7 22.607073231991006 35.1114087859253 20.858499479733528 21.423018502350168 8 20.136106493164775 36.8516104340338 22.708734496657137 20.303548576144287 9 19.98421260360479 18.91258327253591 19.65770054179474 41.44550358206456 10-11 20.631854660271973 25.423090263242877 30.467283013000685 23.47777206348447 12-13 22.99817009723601 20.765808326655584 27.972395976605952 28.263625599502458 14-15 22.10235495329562 23.36295463515566 22.519166138426762 32.01552427312196 16-17 23.554317015703674 27.77505352166581 22.537106361603136 26.13352310102738 18-19 22.353518077764885 27.26196313882145 23.25650931097583 27.128009472437835 20-21 21.1814234969083 25.50441927497578 23.00056212699286 30.31359510112306 22-23 22.526940235136525 29.566085802107377 23.600961595962254 24.30601236679384 24-25 23.390462977359437 30.804559208716558 23.083685161043405 22.7212926528806 26-27 22.054514358158617 33.50516080420041 23.34680843429692 21.093516403344058 28-29 19.068663214170385 29.65339488823241 29.06076951597278 22.21717238162443 30-31 20.985277056846588 27.849206444128168 28.819772517970122 22.345743981055126 32-33 19.425673655380272 24.673786941909555 32.884429082297785 23.016110320412388 34-35 22.019829926684288 26.313523340230354 31.59273301359869 20.07391371948667 36-37 19.800624319766538 27.060434631806817 31.337383837054933 21.80155721137171 38-39 20.836971211921878 24.99850498140197 28.319838298788437 25.844685507887714 40-41 19.729461434500244 25.17013311645597 30.762100680532466 24.33830476851132 42-43 24.118836038320314 24.03930104890505 31.233330542631947 20.608532370142683 44-45 19.34314862876894 25.93737666096566 29.631268612981543 25.088206097283848 46-47 23.617107796820992 23.333054263195034 28.3706689311215 24.67916900886247 48-49 21.67418162681944 22.668069990790684 28.82096853284855 26.836779849541326 50-51 18.434177321165876 22.852854289507363 32.00057408714164 26.71239430218512 52-53 19.262417624475248 23.133917785937257 35.046823982490345 22.556840607097154 54-55 17.79131932401239 23.345612419418497 32.77738575067874 26.08568250589037 56-57 22.08142469292318 22.700960399947377 30.153329107414095 25.064285799715346 58-59 18.44254942531485 27.69133248017605 31.66150386910813 22.204614225400967 60-61 20.072119697169033 32.9472198634151 25.591728361100813 21.388932078315054 62-63 16.628194854743995 37.31506619942352 24.30122830728014 21.755510638552344 64-65 16.29630072598103 38.51885517455837 23.17518029924292 22.009663800217673 66-67 16.63895898864982 37.86284101374221 23.12733970410592 22.37086029350205 68-69 16.664075301096744 36.515530253196346 23.2266089390152 23.593785506691702 70-71 17.18434177321166 35.0779203693294 23.532788747892024 24.204949109566922 72-73 17.43371087536329 32.77798375811795 23.86827092129026 25.920034445228495 74-75 17.263278755187713 32.9376517443877 23.890397196541123 25.908672303883463 76 16.94992285704034 33.057851239669425 24.038703041465837 25.953522861824403 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.0 10 0.5 11 0.0 12 0.0 13 1.0 14 3.0 15 14.0 16 23.0 17 22.0 18 20.0 19 29.0 20 58.5 21 77.0 22 88.0 23 118.5 24 165.0 25 192.0 26 253.5 27 345.5 28 436.0 29 496.0 30 612.0 31 818.0 32 1091.5 33 1275.0 34 1439.0 35 1852.0 36 2412.5 37 2724.0 38 3781.5 39 5784.0 40 6870.0 41 6855.5 42 6700.0 43 6318.5 44 5857.5 45 5593.5 46 5409.0 47 5299.0 48 5012.5 49 4490.5 50 4145.0 51 3861.0 52 3245.0 53 2645.0 54 2377.0 55 2228.5 56 1778.5 57 1323.5 58 1170.0 59 998.0 60 674.5 61 424.0 62 325.0 63 296.5 64 220.5 65 133.5 66 65.0 67 36.0 68 28.0 69 18.0 70 14.0 71 12.0 72 7.5 73 2.5 74 1.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 83611.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.99381660307854 #Duplication Level Percentage of deduplicated Percentage of total 1 66.39519495062608 31.201636148353685 2 16.514812175506464 15.521881092200788 3 7.026875699888017 9.906591237995 4 3.318741728596152 6.238413605865257 5 1.9520513081543318 4.586717058760211 6 1.160541586073501 3.27229670737104 7 0.8551359055278427 2.8130269940558064 8 0.6159014557670772 2.3154848046309695 9 0.45810852081848724 1.937544103048642 >10 1.6517357222844344 10.988984702969704 >50 0.02290542604092436 0.7941538792742582 >100 0.02036037870304388 2.101398141392879 >500 0.002545047337880485 1.0309648252024255 >1k 0.00509009467576097 7.2909066988793345 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT 3122 3.7339584504431236 No Hit ATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTT 2974 3.5569482484362105 No Hit CTTTACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC 862 1.0309648252024255 No Hit CTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC 420 0.5023262489385368 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG 352 0.42099723720563087 No Hit TATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCT 287 0.34325627010800014 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC 175 0.20930260372439033 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC 162 0.19375441030486418 No Hit CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATATCGTATGCCGT 136 0.16265802346581193 No Hit CTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG 116 0.13873772589731015 No Hit TCTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC 109 0.13036562174833455 No Hit CTTAACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC 92 0.11003336881510806 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0023920297568501754 0.0 2 0.0 0.0 0.0 0.008372104148975613 0.0 3 0.0 0.0 0.0 0.008372104148975613 0.0 4 0.0 0.0 0.0 0.008372104148975613 0.0 5 0.0 0.0 0.0 0.008372104148975613 0.0 6 0.0 0.0 0.0 0.009568119027400701 0.0 7 0.0 0.0 0.0 0.009568119027400701 0.0 8 0.0 0.0 0.0 0.009568119027400701 0.0 9 0.0 0.0 0.0 0.010764133905825788 0.0 10 0.0 0.0 0.0 0.010764133905825788 0.0 11 0.0 0.0 0.0 0.010764133905825788 0.0 12 0.0 0.0 0.0 0.013156163662675963 0.0 13 0.0 0.0 0.0 0.014352178541101051 0.0 14 0.0 0.0 0.0 0.016744208297951226 0.0 15 0.0 0.0 0.0 0.019136238054801403 0.0 16 0.0 0.0 0.0 0.020332252933226488 0.0 17 0.0 0.0 0.0 0.022724282690076664 0.0 18 0.0 0.0 0.0 0.028704357082202103 0.0 19 0.0 0.0 0.0 0.03588044635275263 0.0 20 0.0 0.0 0.0 0.04305653562330315 0.0 21 0.0 0.0 0.0 0.04544856538015333 0.0 22 0.0 0.0 0.0 0.04544856538015333 0.0 23 0.0 0.0 0.0 0.047840595137003505 0.0 24 0.0 0.0 0.0 0.05142863977227877 0.0 25 0.0 0.0 0.0 0.05980074392125438 0.0 26 0.0 0.0 0.0 0.06817284807023 0.0 27 0.0 0.0 0.0 0.07295690758393035 0.0 28 0.0 0.0 0.0 0.08132901173290595 0.0 29 0.0 0.0 0.0 0.11122938369353315 0.0 30 0.0 0.0 0.0 0.12916960686990947 0.0 31 0.0 0.0 0.0 0.16983411273636245 0.0 32 0.0 0.0 0.0 0.2200667376302161 0.0 33 0.0 0.0 0.0 0.2702993625240698 0.0 34 0.0 0.0 0.0 0.33129612132374925 0.0 35 0.0 0.0 0.0 0.42697731159775626 0.0 36 0.0 0.0 0.0 0.587243305306718 0.0 37 0.0 0.0 0.0 0.8575426678307878 0.0 38 0.0 0.0 0.0 1.1936228486682374 0.0 39 0.0 0.0 0.0 1.6720288000382724 0.0 40 0.0 0.0 0.0 2.2042554209374363 0.0 41 0.0 0.0 0.0 2.7400700864718757 0.0 42 0.0 0.0 0.0 3.304589109088517 0.0 43 0.0 0.0 0.0 3.8738921912188586 0.0 44 0.0 0.0 0.0 4.408510841874873 0.0 45 0.0 0.0 0.0 4.955089641315138 0.0 46 0.0 0.0 0.0 5.517216634174929 0.0 47 0.0 0.0 0.0 6.091303775818971 0.0 48 0.0 0.0 0.0 6.680939110882539 0.0 49 0.0 0.0 0.0 7.277750535216658 0.0 50 0.0 0.0 0.0 7.8853260934566025 0.0 51 0.0 0.0 0.0 8.501273755845522 0.0 52 0.0 0.0 0.0 9.120809462869719 0.0 53 0.0 0.0 0.0 9.606391503510304 0.0 54 0.0 0.0 0.0 10.15297030295057 0.0 55 0.0 0.0 0.0 10.676824819700757 0.0 56 0.0 0.0 0.0 11.18513114303142 0.0 57 0.0 0.0 0.0 11.713769719295309 0.0 58 0.0 0.0 0.0 12.230448146774945 0.0 59 0.0 0.0 0.0 12.744734544497733 0.0 60 0.0 0.0 0.0 13.269785076126347 0.0 61 0.0 0.0 0.0 13.77569936970016 0.0 62 0.0 0.0 0.0 14.322278169140423 0.0 63 0.0 0.0 0.0 14.81025223953786 0.0 64 0.0 0.0 0.0 15.295834280178445 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGCGCT 15 0.002217675 70.00001 38 TTTCGTG 15 0.002217675 70.00001 24 TTCGTGC 15 0.002217675 70.00001 25 AGCGCTA 15 0.002217675 70.00001 39 ACAGCGC 15 0.002217675 70.00001 37 CACAGCG 15 0.002217675 70.00001 36 TTTCTGC 15 0.002217675 70.00001 51 CTATAAC 25 2.3781901E-4 56.000004 3 GACTTAA 20 0.006929624 52.499996 1 CTTTATA 20 0.006929624 52.499996 2 TGGCTTA 20 0.006929624 52.499996 39 CTGGATG 20 0.006929624 52.499996 58 ATCTGAT 20 0.006929624 52.499996 40 TTTACAC 145 0.0 50.689655 2 CTATACA 80 0.0 48.124996 1 TTACACA 155 0.0 47.419353 3 CTTTACA 160 0.0 45.937496 1 TATATCG 55 1.293156E-7 44.545452 36 CGTTATA 55 1.293156E-7 44.545452 33 ATATCGT 55 1.293156E-7 44.545452 37 >>END_MODULE