FastQCFastQC Report
Wed 25 May 2016
SRR1780519_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780519_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784354
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGTGGTC36780.46892092091071125No Hit
ATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGTGGGC17270.22018119369570371No Hit
CTTTACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGTGG10230.1304258026350347No Hit
ATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGTGGGG9870.1258360383194323No Hit
CTATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGTGG9250.1179314442203393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT6800.032.64715643
TCGGTGG13400.029.8537942
GCGTGTA14550.028.55714831
CTCGGTG13300.028.09507441
ACGAGAG16000.027.95877521
CGAGAGG16350.027.86020322
CGAAGGA407.04321E-427.49110825
CTGCGTG15700.027.31077829
CGTAGCG655.841557E-727.12181315
GCTGCGT16100.027.0402528
TGCGTGT15450.026.8987730
GGTGGTC6950.026.88233844
GGCTGCG16350.026.49062227
TCTCGGT13400.026.4394740
CGTGTAG17200.025.30770532
GATCTCG15700.025.08909838
GTAGATC15600.024.96780435
ATCTCGG15700.024.94893539
TAGATCT15500.024.84494436
GAGAGGC18200.024.78802123
Produced by FastQC (version 0.11.5)