FastQCFastQC Report
Wed 25 May 2016
SRR1780519_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780519_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784354
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT351874.486112138141706No Hit
ATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCTT284613.6285911718433264RNA PCR Primer, Index 43 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC74360.9480413180783166No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG49060.6254828814540373No Hit
CTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC42020.5357274903933683No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC26760.3411724807931113No Hit
TATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCT26070.33237543252154RNA PCR Primer, Index 43 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC17810.22706584016910733No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG11850.15107974205524546No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC9990.1273659597579664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGTT207.8566885E-443.99802424
CGTCTTC14000.039.9099518
CTTGAAA13400.039.8937327
CTGCTTG13600.039.79232824
TCTTCTG14200.038.57327320
TTGAAAA14100.038.06678428
GTCTTCT14700.038.00947619
CTTCTGC15150.037.02803821
TCTGCTT15650.035.84503623
TTCTGCT16100.035.11643622
TGAAAAA15950.034.06526629
TTTACAC9950.034.0530662
TGCTTGA15650.033.73650425
GCTTGAA15950.033.23988726
GATCTTA401.8103161E-533.023781
CTATACA6200.032.6686861
GAAAAAA17950.030.1471430
CCGTCTT43650.030.03951544
TTACACA11200.029.859613
CTTTACA11650.029.6694341
Produced by FastQC (version 0.11.5)