FastQCFastQC Report
Wed 25 May 2016
SRR1780518_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780518_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80737
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGTT150.002218018569.9950468
GTCAATT200.00693069552.49628413
GTATCAA200.00693069552.49628434
TACTCGC200.00693069552.49628420
TATACCG350.001249279839.9971666
GGCCCAT459.1298716E-538.886146
GGAGTAT459.1298716E-538.8861431
GCTATCA400.002407009234.9975221
TTACTCG400.002407009234.9975219
CATCAGG400.002407009234.9975226
GCAAACC450.00428647731.1089122
AGCAAAC500.00717381127.99801621
CCGCCAT500.00717381127.99801610
TATCAAT900.005237445219.4430735
CGATGGG1702.0719472E-618.528170
CAATTTC950.007157021718.41974815
CCATCTT950.007157021718.41974813
CTCCTAA1159.646861E-418.3505331
GATGGGA1159.974797E-418.25957570
ACGATGG2551.0768417E-917.84187370
Produced by FastQC (version 0.11.5)