FastQCFastQC Report
Wed 25 May 2016
SRR1780518_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780518_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80737
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT19542.4202038718307595No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT16532.0473884340513027Illumina PCR Primer Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC3940.4880042607478603No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG2870.35547518485948204No Hit
CTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC2280.2823984046967314No Hit
TATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCT1440.17835688717688294No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC1330.16473240273975995No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC1200.14863073931406914No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGC150.002217395770.023
ACCGGCT150.002217395770.024
CCGGCTT150.002217395770.025
TAACCGG150.002217395770.022
AATCTGC150.002217395770.024
GTTCCAC150.002217395770.059
GTGGTAC150.002217395770.014
GCTTTTT150.002217395770.028
TGGTACC150.002217395770.015
GGAACCC252.3776933E-456.00000436
CGGCTTT200.00692875552.526
TTTGCCC200.00692875552.532
TTGGGCA200.00692875552.521
ATATCGT305.8467896E-446.66666434
CTATACA659.828E-943.0769231
TATATCG350.001248844940.033
ATCGTAT350.001248844940.036
TATCGTA400.002406173635.035
GAGGAAG450.004284992331.11111329
TTTACAC802.431283E-630.6252
Produced by FastQC (version 0.11.5)