##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780518_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 80737 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.01539566741395 34.0 34.0 34.0 32.0 34.0 2 33.338593210052395 34.0 34.0 34.0 33.0 34.0 3 33.479111188178905 34.0 34.0 34.0 34.0 34.0 4 33.50494816502967 34.0 34.0 34.0 34.0 34.0 5 33.5092956141546 34.0 34.0 34.0 34.0 34.0 6 37.251272650705374 38.0 37.0 38.0 36.0 38.0 7 36.84496575300048 38.0 37.0 38.0 35.0 38.0 8 36.910326120613846 38.0 37.0 38.0 35.0 38.0 9 36.69544322925053 38.0 37.0 38.0 34.0 38.0 10-11 36.41869279264773 38.0 37.0 38.0 34.0 38.0 12-13 36.27632931617474 38.0 37.0 38.0 34.0 38.0 14-15 36.51530277320188 38.0 37.0 38.0 34.0 38.0 16-17 36.63025626416636 38.0 37.0 38.0 34.0 38.0 18-19 36.66676368951039 38.0 37.0 38.0 34.0 38.0 20-21 36.41666769882458 38.0 37.0 38.0 34.0 38.0 22-23 36.468998104958075 38.0 37.0 38.0 34.0 38.0 24-25 35.75373125085153 38.0 36.5 38.0 31.5 38.0 26-27 35.93909855456606 38.0 37.0 38.0 32.5 38.0 28-29 35.84035200713427 38.0 37.0 38.0 31.5 38.0 30-31 35.57299007889815 38.0 36.0 38.0 31.5 38.0 32-33 36.09623221075839 38.0 37.0 38.0 34.0 38.0 34-35 36.069373397574836 38.0 37.0 38.0 33.0 38.0 36-37 36.17116687516257 38.0 37.0 38.0 34.0 38.0 38-39 35.78646097823798 38.0 36.5 38.0 32.5 38.0 40-41 35.38211105193406 38.0 36.0 38.0 29.0 38.0 42-43 35.85434806841968 38.0 36.5 38.0 32.5 38.0 44-45 36.05262766761213 38.0 37.0 38.0 33.5 38.0 46-47 36.31158576612953 38.0 37.0 38.0 34.0 38.0 48-49 36.06783754660193 38.0 37.0 38.0 33.0 38.0 50-51 36.202032525360124 38.0 37.0 38.0 34.0 38.0 52-53 36.1208244051674 38.0 37.0 38.0 34.0 38.0 54-55 36.32069559185999 38.0 37.0 38.0 34.0 38.0 56-57 36.33478454735747 38.0 37.0 38.0 34.0 38.0 58-59 36.40534699084682 38.0 37.0 38.0 34.0 38.0 60-61 36.11055030531231 38.0 37.0 38.0 33.0 38.0 62-63 36.40172411657605 38.0 37.0 38.0 34.0 38.0 64-65 36.42777165364083 38.0 37.0 38.0 34.0 38.0 66-67 35.729157635284935 38.0 36.5 38.0 31.0 38.0 68-69 34.88077337528023 38.0 35.5 38.0 26.0 38.0 70-71 34.610147763726665 38.0 35.0 38.0 25.0 38.0 72-73 34.1270421244287 38.0 34.5 38.0 23.5 38.0 74-75 34.63178592219181 38.0 35.5 38.0 24.5 38.0 76 34.10293917286994 37.0 34.0 38.0 24.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1728465843039686 1101 2 0.4406047320740001 1101 3 0.1536458019345801 1101 4 0.3579997281813476 1101 5 0.34950431923013525 1101 6 -1.6512216962399577 1101 7 0.21336847283075855 1101 8 0.29151103302424275 1101 9 0.49553758886634824 1101 10-11 0.25958663416425765 1101 12-13 0.9828461483185862 1101 14-15 0.7794167773133793 1101 16-17 -0.01393964002361514 1101 18-19 -0.22778936990951593 1101 20-21 -0.09955664003209819 1101 22-23 0.6327888268669923 1101 24-25 -1.9845455555085394 1101 26-27 -0.42201400149486545 1101 28-29 0.5988374113085015 1101 30-31 1.1808808967319493 1101 32-33 0.181264184547679 1101 34-35 0.17624211592925576 1101 36-37 0.1268693673594541 1101 38-39 0.23547894811006387 1101 40-41 1.2228255160312074 1101 42-43 0.6857594580494464 1101 44-45 0.06537731175726691 1101 46-47 0.7268851138349604 1101 48-49 -1.8604373377998016 1101 50-51 -1.762152496894231 1101 52-53 -1.759001325612239 1101 54-55 -0.8690715616741613 1101 56-57 -1.006395923353466 1101 58-59 -1.4515755814878304 1101 60-61 -1.631255361944909 1101 62-63 -0.2846326720206136 1101 64-65 -1.4903719180111565 1101 66-67 -2.1715423515642414 1101 68-69 -1.3910363312195315 1101 70-71 -1.6004954814867176 1101 72-73 -2.4211021221910016 1101 74-75 -1.3982380190991819 1101 76 -0.7436531943933034 1102 1 -0.8030328126809536 1102 2 -0.10828606336662006 1102 3 -0.0053197575702625954 1102 4 0.15177347496403826 1102 5 0.02708735636703352 1102 6 -0.04300990557933204 1102 7 -0.6027364419872328 1102 8 0.23270480677102512 1102 9 0.2720543188577693 1102 10-11 0.2669282201429368 1102 12-13 0.4512491388811597 1102 14-15 -0.4314791658169028 1102 16-17 -0.1511745952877348 1102 18-19 -0.2541161256393849 1102 20-21 0.10751530505920925 1102 22-23 0.5783336240235073 1102 24-25 -4.525592252407673 1102 26-27 -1.2448476820929812 1102 28-29 -0.049955332900353255 1102 30-31 1.4625085471793042 1102 32-33 -0.007913410745864269 1102 34-35 0.3379163407117076 1102 36-37 -2.70317107877365 1102 38-39 -0.08232960685990776 1102 40-41 1.0011807066182854 1102 42-43 0.692929454372532 1102 44-45 0.9974645773535329 1102 46-47 0.7738884230640295 1102 48-49 -3.2251511507670827 1102 50-51 -0.7885527910479269 1102 52-53 -0.29872433088249295 1102 54-55 0.38532235914946256 1102 56-57 0.8229213949453396 1102 58-59 -0.014928933614321238 1102 60-61 -5.834147873282085 1102 62-63 -2.9573499167809985 1102 64-65 -0.3404270718290192 1102 66-67 0.6384343612682102 1102 68-69 -1.026972107662722 1102 70-71 -1.6373750010356858 1102 72-73 -4.906664078313078 1102 74-75 -1.4284377984839054 1102 76 -0.5305878677601328 1103 1 0.7427298991834519 1103 2 0.6317511420901809 1103 3 0.04718127591465304 1103 4 0.338772234782148 1103 5 0.4112750372268934 1103 6 0.20279414568151566 1103 7 0.9215917928205357 1103 8 0.8063096021410132 1103 9 0.7115541121607833 1103 10-11 0.37977443758816065 1103 12-13 -0.2994019566128472 1103 14-15 -0.11969743783013342 1103 16-17 0.39536323026000986 1103 18-19 0.5146622951956701 1103 20-21 -0.006943148847362579 1103 22-23 0.4353716562682379 1103 24-25 -2.695590185437851 1103 26-27 -0.5644528908569271 1103 28-29 0.12242995027450831 1103 30-31 1.0397408745873236 1103 32-33 1.363882786029663 1103 34-35 0.609009767747672 1103 36-37 -0.5012074574425185 1103 38-39 0.5006075572575028 1103 40-41 -0.025886263203943827 1103 42-43 -0.16672329469733427 1103 44-45 1.011974705505665 1103 46-47 1.0461083486531138 1103 48-49 -2.094797698927472 1103 50-51 -0.2976371234166706 1103 52-53 0.5582310225693448 1103 54-55 -0.06216627251650664 1103 56-57 0.441606802138395 1103 58-59 -0.13097895428401785 1103 60-61 -3.6608737930836526 1103 62-63 -0.7147600035937316 1103 64-65 0.5755402700477887 1103 66-67 0.4673512690813624 1103 68-69 1.0555930018866704 1103 70-71 0.8682678265790358 1103 72-73 -3.7957401428025364 1103 74-75 -1.035289803444634 1103 76 -1.8255031219974214 1104 1 0.046967187319040704 1104 2 0.25501770603471385 1104 3 0.4056558521858378 1104 4 -0.43503362037964166 1104 5 -0.3133782755620089 1104 6 -0.1676218789717936 1104 7 0.6085717620038977 1104 8 0.5383589087665896 1104 9 0.8144046514520014 1104 10-11 -0.4085152388371043 1104 12-13 0.3138877198124206 1104 14-15 0.6177447809680174 1104 16-17 0.2237145399672542 1104 18-19 0.5113495062896618 1104 20-21 -0.18444700092749144 1104 22-23 0.43814515395698805 1104 24-25 0.2804498761954335 1104 26-27 1.050107972009016 1104 28-29 1.3135701659910026 1104 30-31 0.6130074385318522 1104 32-33 0.4154236180789681 1104 34-35 0.08192964448110018 1104 36-37 0.8482147305389915 1104 38-39 1.3444442290602723 1104 40-41 1.4885975580595385 1104 42-43 0.6148252415122215 1104 44-45 0.5132073711451071 1104 46-47 0.5608618155252287 1104 48-49 -0.18740170509080656 1104 50-51 0.4881101801272436 1104 52-53 0.4034929640177296 1104 54-55 0.3105995210119943 1104 56-57 0.1278548760983398 1104 58-59 -0.7570575367185342 1104 60-61 0.5365838340349924 1104 62-63 0.34525540472676397 1104 64-65 0.8350163871510219 1104 66-67 -0.16855011766747907 1104 68-69 1.5872185796986926 1104 70-71 1.128090630893368 1104 72-73 0.37825831636541096 1104 74-75 -0.6399122995925595 1104 76 -0.6533151404874076 1105 1 0.6721808625327199 1105 2 0.2210239220409278 1105 3 0.42745072398070505 1105 4 -0.26498700033302214 1105 5 -0.20914362132735675 1105 6 0.09515978380986923 1105 7 0.7913223447544837 1105 8 -0.5168469964393125 1105 9 0.4367822738296283 1105 10-11 0.5125609072390418 1105 12-13 -0.248427742503047 1105 14-15 0.16995923318247463 1105 16-17 0.05360964486235531 1105 18-19 0.3043370742772282 1105 20-21 0.080102338621856 1105 22-23 0.058560849372682355 1105 24-25 0.5339658047113645 1105 26-27 0.5734762703773129 1105 28-29 0.6026921551129902 1105 30-31 0.9292273297517468 1105 32-33 0.6108587385140893 1105 34-35 -0.5798030672516035 1105 36-37 0.11330797063222775 1105 38-39 -0.18067232105627795 1105 40-41 0.21406375852573944 1105 42-43 0.01406766575464502 1105 44-45 0.7257947837325176 1105 46-47 0.8050537347171485 1105 48-49 -0.06508235777145899 1105 50-51 0.48113659815366105 1105 52-53 0.20217206269682464 1105 54-55 0.6560540664665382 1105 56-57 0.5743389475824046 1105 58-59 0.584706255045262 1105 60-61 0.7506863981375531 1105 62-63 -0.45503597056461587 1105 64-65 0.35710263423727184 1105 66-67 0.9257389266215625 1105 68-69 0.579875922356031 1105 70-71 0.6415910193937506 1105 72-73 0.8777532658603633 1105 74-75 -0.26126039594065276 1105 76 0.47624973907747403 1106 1 -1.0499811694884684 1106 2 0.07181241367449331 1106 3 0.15189998364123625 1106 4 0.07015203903137035 1106 5 -0.020868672489029905 1106 6 0.09648396823263994 1106 7 -0.588056492370832 1106 8 0.20483351397358973 1106 9 -0.3678403336098839 1106 10-11 0.3895321363229485 1106 12-13 1.2037511427744292 1106 14-15 -0.11516174912160437 1106 16-17 0.5143355386868436 1106 18-19 0.8328070393669122 1106 20-21 0.4507535965970533 1106 22-23 0.2358510865220751 1106 24-25 -0.3083358685762292 1106 26-27 0.906990174313755 1106 28-29 0.4634421250178917 1106 30-31 0.530858484199797 1106 32-33 0.005255030797705729 1106 34-35 0.6918955002216123 1106 36-37 0.7002527160276841 1106 38-39 -0.20513963777711552 1106 40-41 0.5537170629677775 1106 42-43 0.5422366798391494 1106 44-45 0.912865928550147 1106 46-47 1.033535737847032 1106 48-49 1.1890707660999809 1106 50-51 0.030673133605681357 1106 52-53 0.8075049145085558 1106 54-55 -0.11859382085740577 1106 56-57 0.314527944813662 1106 58-59 0.561081601138909 1106 60-61 1.0797763222979029 1106 62-63 0.9339131085253101 1106 64-65 -0.7639699551451784 1106 66-67 0.2984366514319632 1106 68-69 1.1957119642484102 1106 70-71 0.06785802312554523 1106 72-73 -0.06629994227735381 1106 74-75 -0.8502516081712983 1106 76 -0.5155643935817338 1107 1 0.6795758829712142 1107 2 -0.3957728077992826 1107 3 0.19913411305540052 1107 4 0.20903752586541913 1107 5 -0.021481042360427693 1107 6 0.45688404988195686 1107 7 0.7848563469841352 1107 8 -0.01045532048242137 1107 9 -0.8618799335282361 1107 10-11 0.5068009666964883 1107 12-13 -0.026428485721176287 1107 14-15 -0.07257142456557375 1107 16-17 0.5653153304810914 1107 18-19 0.8466261861315587 1107 20-21 0.7022130781238971 1107 22-23 0.12223606304789314 1107 24-25 0.24853288014799801 1107 26-27 -0.1065125813506711 1107 28-29 0.8121867667815152 1107 30-31 0.5567821420891619 1107 32-33 1.1394354757864846 1107 34-35 -0.07380158081533494 1107 36-37 0.7070091969421455 1107 38-39 0.3125272330128439 1107 40-41 0.3499611944231731 1107 42-43 0.489429984595219 1107 44-45 0.7124168573111191 1107 46-47 0.8060199182920229 1107 48-49 0.500246516253064 1107 50-51 0.8336635398110346 1107 52-53 0.6106075885236564 1107 54-55 0.3821410229882787 1107 56-57 0.8706414373631617 1107 58-59 0.6338515541905565 1107 60-61 1.0322360079480362 1107 62-63 0.9823107406951337 1107 64-65 0.9849175729217663 1107 66-67 0.38382142383449036 1107 68-69 0.5949260895801132 1107 70-71 -0.160545732742996 1107 72-73 0.7490093045077089 1107 74-75 -1.0141217857585616 1107 76 0.021586045283342514 1108 1 -0.45085889963748116 1108 2 0.46074893133115324 1108 3 0.24696020001192664 1108 4 0.40903752586541486 1108 5 0.07851895763957373 1108 6 -0.38659421098760305 1108 7 -0.3542740877984727 1108 8 -0.4713248856998149 1108 9 -0.10970602048475797 1108 10-11 0.5394096623486675 1108 12-13 1.195310644713608 1108 14-15 0.3100372710865926 1108 16-17 -0.04772814777978596 1108 18-19 0.3857566209141652 1108 20-21 0.9630826433412878 1108 22-23 -0.2473291543434044 1108 24-25 0.935489401887132 1108 26-27 -0.1934691030897966 1108 28-29 -0.6225958419141335 1108 30-31 0.01765170730655541 1108 32-33 0.6894354757864818 1108 34-35 0.4044592887498837 1108 36-37 1.0700526752030228 1108 38-39 0.8190489721432783 1108 40-41 0.8499611944231731 1108 42-43 -0.01491784149173725 1108 44-45 0.6667646833980783 1108 46-47 0.5951503530746294 1108 48-49 0.9567682553835013 1108 50-51 0.5945331050284253 1108 52-53 -0.12852284625895294 1108 54-55 -0.17872854222910917 1108 56-57 0.3380327417109896 1108 58-59 0.4360254672340318 1108 60-61 0.888757747078472 1108 62-63 0.7410063928690462 1108 64-65 0.5370914859652487 1108 66-67 1.144690989051881 1108 68-69 1.421013046101855 1108 70-71 -0.3692413849169114 1108 72-73 -0.0335993911444703 1108 74-75 -0.6162956988020412 1108 76 0.4737599583268235 1109 1 -1.2850840947322375 1109 2 0.21675041776742177 1109 3 0.3077926043225858 1109 4 0.05125231590629653 1109 5 -0.004015416199152355 1109 6 -0.34928466063458075 1109 7 -0.20013064669851133 1109 8 0.3207598411675221 1109 9 0.31712415417150197 1109 10-11 0.11939851407664293 1109 12-13 0.2067004626251574 1109 14-15 0.5695318827551219 1109 16-17 0.6262592175119295 1109 18-19 0.5137387836789387 1109 20-21 0.5480510565705714 1109 22-23 0.23377452458635872 1109 24-25 -0.5493675286219712 1109 26-27 0.01792071482176283 1109 28-29 -1.4485898961690609 1109 30-31 0.04247519299960345 1109 32-33 0.25829463594998714 1109 34-35 -0.3020252894738249 1109 36-37 0.7265558338800915 1109 38-39 1.030866140482182 1109 40-41 0.47047401493598784 1109 42-43 0.531161682848662 1109 44-45 0.43519649313423514 1109 46-47 0.46531014497355727 1109 48-49 0.05671251402340971 1109 50-51 -0.553051436034373 1109 52-53 -0.6931270826023166 1109 54-55 -0.5042023437898706 1109 56-57 -0.09019096694750317 1109 58-59 0.07188574222475097 1109 60-61 0.6117975092486674 1109 62-63 0.16034864481999733 1109 64-65 0.1733419504765905 1109 66-67 -0.08067132978870006 1109 68-69 -0.6188420263619108 1109 70-71 0.10312948093221763 1109 72-73 -0.7354945973874933 1109 74-75 -0.690747575427828 1109 76 -0.7374254745977424 1110 1 -0.27905091222847034 1110 2 0.044301951201312306 1110 3 0.2615382051270103 1110 4 0.10303713239699164 1110 5 0.30116306544992 1110 6 0.18967964303561047 1110 7 0.5522574880425637 1110 8 0.6631429482565139 1110 9 0.8983207353680882 1110 10-11 -0.1234370817001249 1110 12-13 0.6053932730826759 1110 14-15 0.8026892482654304 1110 16-17 0.3873200521021758 1110 18-19 -0.009639798523167542 1110 20-21 0.8641646815077308 1110 22-23 -0.5184879188525571 1110 24-25 -0.83531524104027 1110 26-27 1.13380809541502 1110 28-29 -0.07164168098555024 1110 30-31 0.030911593200713128 1110 32-33 0.727877340826808 1110 34-35 0.6084825033869024 1110 36-37 1.1151933401646588 1110 38-39 0.44564743761642234 1110 40-41 -0.818364597432037 1110 42-43 0.03820039576972789 1110 44-45 0.8570165031895343 1110 46-47 0.8445208035959837 1110 48-49 1.356109195773037 1110 50-51 -0.2003616421681116 1110 52-53 0.5802514995997967 1110 54-55 -0.18783733624839272 1110 56-57 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38-39 -3.443112026565288 2116 40-41 -1.0802368253788046 2116 42-43 -0.6880775487668132 2116 44-45 0.04948099850784615 2116 46-47 -0.6787196426205853 2116 48-49 0.0770222373034315 2116 50-51 -1.003271458036572 2116 52-53 -0.29524690996966996 2116 54-55 -0.36869840878098614 2116 56-57 -0.32567797718699154 2116 58-59 -2.37109894085895 2116 60-61 -1.5535151758659964 2116 62-63 0.24406278546483406 2116 64-65 -2.0553751520028953 2116 66-67 -0.34717297995371155 2116 68-69 -1.9191720593652164 2116 70-71 0.40183050488076333 2116 72-73 0.23721421195497072 2116 74-75 0.30425961759916476 2116 76 -1.1206868776611216 2117 1 0.47989401658733755 2117 2 0.5648846683406958 2117 3 0.360533900966324 2117 4 5.539627689188364E-4 2117 5 -0.192424838754917 2117 6 0.2740632651523711 2117 7 0.5045806311835008 2117 8 0.008314562868584119 2117 9 -1.1084334860202816 2117 10-11 -0.8111205603448681 2117 12-13 -1.8316776903871386 2117 14-15 0.0636427854026067 2117 16-17 -1.3170282538243185 2117 18-19 -1.4492380768589044 2117 20-21 -0.33882615835542396 2117 22-23 -0.48508101012283333 2117 24-25 -2.0012020085052384 2117 26-27 -2.3716769503856625 2117 28-29 -2.0036138694857186 2117 30-31 -1.0204182820889827 2117 32-33 -2.1884012156239123 2117 34-35 0.48669682851658536 2117 36-37 -1.5714637617004783 2117 38-39 -0.2506222897867332 2117 40-41 -0.408575390942687 2117 42-43 -0.30950957001771684 2117 44-45 -0.7000963982562212 2117 46-47 -0.9748920647408497 2117 48-49 -1.0422243214987859 2117 50-51 -0.1275771812918336 2117 52-53 0.279324449605312 2117 54-55 -0.6634581286554138 2117 56-57 -0.12098104407904486 2117 58-59 -1.5320021043460343 2117 60-61 -0.7780503123064761 2117 62-63 -0.28661820946393135 2117 64-65 -1.2721343889021952 2117 66-67 -1.1713217362927608 2117 68-69 -0.06599649790663165 2117 70-71 -0.4081067083527543 2117 72-73 0.9518725070527765 2117 74-75 0.42522073810145145 2117 76 0.4547779858984029 2118 1 -0.2614060184225835 2118 2 0.4906636390490675 2118 3 -0.8558274014026779 2118 4 0.06711948966820813 2118 5 0.34236335123225103 2118 6 0.13094271432964177 2118 7 0.9238827667885872 2118 8 0.6323780475225718 2118 9 -0.7693274059642619 2118 10-11 -0.3392568656313628 2118 12-13 -0.3160165864076774 2118 14-15 0.8828165513029447 2118 16-17 -2.6625294791715035 2118 18-19 -0.4191528860548317 2118 20-21 -0.06805944111887641 2118 22-23 0.8499872592201427 2118 24-25 0.7824833690184008 2118 26-27 -1.4593417887565323 2118 28-29 -0.13642983400900022 2118 30-31 0.07987346723999167 2118 32-33 0.7739164410454791 2118 34-35 0.3089631373040689 2118 36-37 -2.3385077574992863 2118 38-39 -0.07672801974354826 2118 40-41 -0.017502920409370404 2118 42-43 0.6448491649571935 2118 44-45 -2.0001897580414933 2118 46-47 0.33323023657786166 2118 48-49 0.6677418355790508 2118 50-51 -0.32444962848519765 2118 52-53 -0.38693765799183666 2118 54-55 -0.07361100530379616 2118 56-57 0.5638596607873296 2118 58-59 -0.5120814601635146 2118 60-61 -0.29547358223295106 2118 62-63 -0.6156297627373561 2118 64-65 0.4533178225577217 2118 66-67 0.5178135186961583 2118 68-69 -0.890771850923322 2118 70-71 -0.3952347279583108 2118 72-73 1.008401857035274 2118 74-75 0.46068578653184744 2118 76 0.6875939504216859 2119 1 0.7959621621934119 2119 2 -1.6146716498355502 2119 3 -0.2684647508292386 2119 4 -0.08421132709503354 2119 5 -0.49471678629750215 2119 6 -0.4200659173454824 2119 7 -0.5705511251591915 2119 8 0.04025475161720493 2119 9 -0.47933678299295224 2119 10-11 -0.6951872351824662 2119 12-13 -0.46439003258593203 2119 14-15 -0.2683219161539583 2119 16-17 -0.30288436364170224 2119 18-19 -0.5142215307135416 2119 20-21 -1.2779485998310918 2119 22-23 -0.4023870525299884 2119 24-25 0.5003790676070352 2119 26-27 0.3317548427262409 2119 28-29 -0.08709223693270474 2119 30-31 0.5828909469831984 2119 32-33 0.08076167198485962 2119 34-35 0.2189556837684492 2119 36-37 -0.17966329595712693 2119 38-39 -2.1841190418396863 2119 40-41 -0.9279282528130111 2119 42-43 0.05761875453185894 2119 44-45 0.0804526886416852 2119 46-47 0.038442902779678434 2119 48-49 -0.4333016594078387 2119 50-51 0.30819926189973756 2119 52-53 -0.22633800902931966 2119 54-55 0.012837986064525353 2119 56-57 -2.403380847976578 2119 58-59 -0.4968017121411066 2119 60-61 -0.21668249762329594 2119 62-63 -0.8700598076990005 2119 64-65 -0.1989146315789938 2119 66-67 0.388759155973446 2119 68-69 0.8225315079764428 2119 70-71 -0.04270653676309877 2119 72-73 0.7799029029346229 2119 74-75 -0.18718274271726898 2119 76 0.5176533382700157 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 2.0 22 7.0 23 16.0 24 45.0 25 91.0 26 170.0 27 299.0 28 511.0 29 806.0 30 1319.0 31 2051.0 32 3040.0 33 4841.0 34 7371.0 35 10959.0 36 17693.0 37 31514.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.426322503932525 20.211303367724835 10.580031460173155 28.782342668169488 2 20.63490097476993 20.132033640090665 35.75436293149362 23.478702453645788 3 18.142858912270707 24.070748231913498 32.138920197678885 25.647472658136916 4 13.060926217223825 20.264562715979043 35.9327198186705 30.74179124812663 5 12.607602462315914 35.83610983811635 36.03180697821321 15.524480721354522 6 25.755229944139614 38.19314564573864 18.53053742398157 17.521086986140183 7 26.322503932521645 31.733901433048047 21.093179087654978 20.850415546775334 8 22.969642171495103 35.03102666683181 21.141484077932052 20.857847083741035 9 22.11006106246207 18.779493912332633 20.24226810508193 38.86817692012337 10-11 22.555333985657132 25.57377658322702 28.454116452184252 23.416772978931593 12-13 23.9394577455194 22.163939705463417 27.244633811016016 26.651968738001163 14-15 22.34848954011172 24.171693275697635 22.941773907873714 30.538043276316927 16-17 23.10217124738348 27.837298884030865 23.850898596678103 25.20963127190755 18-19 22.20914822200478 27.400076792548646 24.815140517978126 25.575634467468443 20-21 21.282683280280416 26.39248423894869 24.529645639545684 27.79518684122521 22-23 22.412276899067344 28.868424638022223 25.204676913930417 23.51462154898002 24-25 22.970261466242242 29.374388446437198 24.85477538179521 22.80057470552535 26-27 21.865439637340998 28.547010664255545 27.95991924396497 21.627630454438485 28-29 20.392137433890287 25.467257886718603 28.763144531008088 25.37746014838302 30-31 19.84901594064679 26.47051537708857 29.768879200366623 23.911589481898016 32-33 19.063750201270793 29.427028499944264 27.546230352874147 23.962990945910796 34-35 20.30110110606042 29.067218251854786 30.07481080545475 20.556869836630046 36-37 20.349406096337493 28.258419312087398 29.380581393908617 22.0115931976665 38-39 21.112377224816377 26.20793440430038 28.501182852967037 24.178505517916197 40-41 20.667104301621315 25.625797341986946 29.75897048441235 23.94812787197939 42-43 23.01361209854218 25.0907266804563 29.890260970806448 22.005400250195077 44-45 19.879361383256747 26.267386700026012 29.462328300531354 24.390923616185887 46-47 22.865600653975253 23.779060406009638 28.796586447353757 24.558752492661355 48-49 21.039300444653627 23.89177204998947 29.699518188686724 25.36940931667018 50-51 18.93679477810669 24.08746919008633 31.423015469982786 25.552720561824195 52-53 19.086044812167906 24.167977507214783 33.37255533398566 23.373422346631656 54-55 18.186828839317783 24.446660143428662 31.70727175892094 25.65923925833261 56-57 20.71726717613981 24.38534996346161 29.864250591426483 25.033132268972096 58-59 18.35775418952896 26.8222747934652 31.236607751093054 23.58336326591278 60-61 19.12010602326071 29.877255781116464 27.68371378673966 23.318924408883163 62-63 17.179236285717824 32.73592033392373 26.39000705996012 23.69483632039833 64-65 17.674052788684246 33.72431475036229 24.810186160000992 23.791446300952476 66-67 17.40218239468893 33.28956983786864 24.153114433283378 25.155133334159057 68-69 16.887548459813964 32.46033417144556 24.82319134969097 25.828926019049508 70-71 17.717403420984184 31.18210981334456 25.03746733220209 26.063019433469165 72-73 17.809678338308334 29.643781661443946 25.225732935333244 27.320807064914476 74-75 18.004136888910907 29.794889579746588 24.768074117195336 27.432899414147172 76 17.986796635990935 29.93546948734781 25.07648290127203 27.00125097538923 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 1.0 13 1.5 14 1.0 15 5.5 16 9.0 17 7.0 18 10.5 19 11.0 20 13.0 21 18.0 22 25.5 23 52.5 24 96.0 25 120.0 26 149.0 27 230.0 28 344.0 29 406.0 30 462.0 31 641.0 32 958.5 33 1153.0 34 1272.5 35 1640.5 36 2166.5 37 2444.0 38 3280.5 39 4792.5 40 5843.5 41 6087.0 42 5955.0 43 5762.0 44 5653.5 45 5649.0 46 5560.0 47 5510.0 48 5338.0 49 4959.0 50 4702.0 51 4404.0 52 3690.0 53 2958.0 54 2642.0 55 2430.0 56 1960.5 57 1466.0 58 1229.0 59 998.0 60 666.0 61 478.0 62 391.0 63 319.5 64 192.0 65 105.0 66 56.0 67 38.0 68 27.0 69 11.0 70 3.5 71 1.0 72 1.0 73 1.0 74 1.0 75 1.0 76 1.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 80737.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.55903736824504 #Duplication Level Percentage of deduplicated Percentage of total 1 62.615882336949134 28.527193232347003 2 16.88551776635946 15.385758697994723 3 8.06622624582008 11.024685088621078 4 4.200309925780931 7.654483074674561 5 2.3924095370143816 5.449793774849201 6 1.4925373134328357 4.079913794171198 7 1.0466791724437918 3.337998687095136 8 0.6932550362939401 2.526722568339175 9 0.527417557023625 2.162577257019706 >10 2.0417040480656823 13.335893084954852 >50 0.01631188320691624 0.4297905545165166 >100 0.01631188320691624 1.6175978795347858 >500 0.0 0.0 >1k 0.005437294402305413 4.467592305882062 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT 1954 2.4202038718307595 No Hit ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT 1653 2.0473884340513027 Illumina PCR Primer Index 2 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC 394 0.4880042607478603 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG 287 0.35547518485948204 No Hit CTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC 228 0.2823984046967314 No Hit TATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCT 144 0.17835688717688294 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC 133 0.16473240273975995 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC 120 0.14863073931406914 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.013624484437123004 0.0 2 0.0 0.0 0.0 0.013624484437123004 0.0 3 0.0 0.0 0.0 0.014863073931406914 0.0 4 0.0 0.0 0.0 0.01610166342569082 0.0 5 0.0 0.0 0.0 0.017340252919974732 0.0 6 0.0 0.0 0.0 0.017340252919974732 0.0 7 0.0 0.0 0.0 0.018578842414258643 0.0 8 0.0 0.0 0.0 0.018578842414258643 0.0 9 0.0 0.0 0.0 0.02353320039139428 0.0 10 0.0 0.0 0.0 0.0260103793799621 0.0 11 0.0 0.0 0.0 0.027248968874246007 0.0 12 0.0 0.0 0.0 0.028487558368529918 0.0 13 0.0 0.0 0.0 0.028487558368529918 0.0 14 0.0 0.0 0.0 0.02972614786281383 0.0 15 0.0 0.0 0.0 0.030964737357097736 0.0 16 0.0 0.0 0.0 0.033441916345665554 0.0 17 0.0 0.0 0.0 0.034680505839949465 0.0 18 0.0 0.0 0.0 0.04087345331136901 0.0 19 0.0 0.0 0.0 0.04335063229993683 0.0 20 0.0 0.0 0.0 0.045827811288504654 0.0 21 0.0 0.0 0.0 0.04706640078278856 0.0 22 0.0 0.0 0.0 0.05945229572562766 0.0 23 0.0 0.0 0.0 0.07059960117418285 0.0 24 0.0 0.0 0.0 0.0780311381398863 0.0 25 0.0 0.0 0.0 0.0792697276341702 0.0 26 0.0 0.0 0.0 0.09041703308272539 0.0 27 0.0 0.0 0.0 0.11890459145125531 0.0 28 0.0 0.0 0.0 0.1424377918426496 0.0 29 0.0 0.0 0.0 0.16597099223404388 0.0 30 0.0 0.0 0.0 0.20189008756827725 0.0 31 0.0 0.0 0.0 0.24895648835106582 0.0 32 0.0 0.0 0.0 0.3133631420538291 0.0 33 0.0 0.0 0.0 0.38148556423944413 0.0 34 0.0 0.0 0.0 0.452085165413627 0.0 35 0.0 0.0 0.0 0.5214461770935259 0.0 36 0.0 0.0 0.0 0.6775084533732985 0.0 37 0.0 0.0 0.0 0.930180710207216 0.0 38 0.0 0.0 0.0 1.3091890954580923 0.0 39 0.0 0.0 0.0 1.7389796499746089 0.0 40 0.0 0.0 0.0 2.3050150488623555 0.0 41 0.0 0.0 0.0 3.091519377732638 0.0 42 0.0 0.0 0.0 3.904034085982883 0.0 43 0.0 0.0 0.0 4.850316459615789 0.0 44 0.0 0.0 0.0 5.8374722865600654 0.0 45 0.0 0.0 0.0 6.617783667958928 0.0 46 0.0 0.0 0.0 7.50213656687764 0.0 47 0.0012385894942839094 0.0 0.0 8.309696917150749 0.0 48 0.0012385894942839094 0.0 0.0 9.09248547753818 0.0 49 0.0012385894942839094 0.0 0.0 9.803435847257143 0.0 50 0.0012385894942839094 0.0 0.0 10.456172510744764 0.0 51 0.0012385894942839094 0.0 0.0 11.185701722877987 0.0 52 0.0012385894942839094 0.0 0.0 12.086156285222389 0.0 53 0.0012385894942839094 0.0 0.0 13.093129544075207 0.0 54 0.0012385894942839094 0.0 0.0 14.137260487756542 0.0 55 0.0012385894942839094 0.0 0.0 15.022851976169537 0.0 56 0.0012385894942839094 0.0 0.0 15.782107336165575 0.0 57 0.0012385894942839094 0.0 0.0 16.442275536618897 0.0 58 0.0012385894942839094 0.0 0.0 17.15941885380928 0.0 59 0.0012385894942839094 0.0 0.0 17.698205283822784 0.0 60 0.0012385894942839094 0.0 0.0 18.318738620459023 0.0 61 0.0012385894942839094 0.0 0.0 18.93307900962384 0.0 62 0.0012385894942839094 0.0 0.0 19.619257589457128 0.0 63 0.0012385894942839094 0.0 0.0 20.350025391084632 0.0 64 0.0012385894942839094 0.0 0.0 21.082031782206425 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCGGC 15 0.0022173957 70.0 23 ACCGGCT 15 0.0022173957 70.0 24 CCGGCTT 15 0.0022173957 70.0 25 TAACCGG 15 0.0022173957 70.0 22 AATCTGC 15 0.0022173957 70.0 24 GTTCCAC 15 0.0022173957 70.0 59 GTGGTAC 15 0.0022173957 70.0 14 GCTTTTT 15 0.0022173957 70.0 28 TGGTACC 15 0.0022173957 70.0 15 GGAACCC 25 2.3776933E-4 56.000004 36 CGGCTTT 20 0.006928755 52.5 26 TTTGCCC 20 0.006928755 52.5 32 TTGGGCA 20 0.006928755 52.5 21 ATATCGT 30 5.8467896E-4 46.666664 34 CTATACA 65 9.828E-9 43.076923 1 TATATCG 35 0.0012488449 40.0 33 ATCGTAT 35 0.0012488449 40.0 36 TATCGTA 40 0.0024061736 35.0 35 GAGGAAG 45 0.0042849923 31.111113 29 TTTACAC 80 2.431283E-6 30.625 2 >>END_MODULE