FastQCFastQC Report
Wed 25 May 2016
SRR1780517_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780517_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433423
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGAC250.002338496335.23867417
CAGTAGT250.002354463335.189841
GAACGTA250.00235579835.18577630
ACATTGC401.8339748E-532.956223
TGGCTAC353.228661E-431.4049933
CGTGCGA951.8189894E-1230.1139610
TGAACGT300.005752346529.32147829
GTCATAG407.03666E-427.4920621
TGTCGGT407.065213E-427.47302243
AATGACG407.079526E-427.4635167
GCATAAG655.9313606E-727.0691071
CGGTGGT508.385096E-526.37410243
CCGTGCG1107.2759576E-1225.9655069
CAGATTA609.634192E-625.70081115
CTGCGTC609.834932E-625.6326149
CGAGAAT1151.2732926E-1124.89976714
GATGTAT1151.2732926E-1124.89400924
CCCGTGC1151.2732926E-1124.836578
CTTTAGT801.3094177E-724.7428551
TAGCCCG1251.8189894E-1224.607315
Produced by FastQC (version 0.11.5)