SRR1780517_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780517_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	433423
Sequences flagged as poor quality	0
Sequence length	50
%GC	47

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT	12400	2.8609464656928685	No Hit
ATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTT	9597	2.2142341315527787	RNA PCR Primer, Index 24 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG	2176	0.5020499604312646	No Hit
CTTTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC	1893	0.4367557789965	No Hit
CTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC	1318	0.3040909227244516	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC	1105	0.25494724553150155	No Hit
TATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCT	834	0.19242172196676227	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC	711	0.16404297879900237	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAT	20	7.805822E-4	44.051636	1
TTCGGAC	30	2.5217305E-6	44.005886	13
CCGTCTT	1000	0.0	42.680782	44
GCCGTCT	1105	0.0	38.625145	43
AGTTCGG	35	7.2730327E-6	37.71933	11
GTTCGGA	40	1.8157296E-5	33.004417	12
CTGTACG	40	1.8171639E-5	33.000607	41
ATCTACG	30	0.0057081836	29.367754	1
TTATCTA	30	0.005740486	29.333872	41
CTTTATA	120	0.0	29.333872	2
TTCGGCA	45	4.0662842E-5	29.330488	25
CCATCGG	30	0.005743724	29.330486	19
TTCCACG	30	0.005743724	29.330486	34
CTATACA	220	0.0	28.032858	1
TGCCGTC	1550	0.0	27.677927	42
CGTAGCG	40	7.019313E-4	27.50368	15
GCGGAAT	40	7.028789E-4	27.497334	19
GAACAAC	40	7.028789E-4	27.497334	35
TAGTTCG	50	8.321735E-5	26.403532	10
CCTGTAC	50	8.3282706E-5	26.400486	40