FastQCFastQC Report
Wed 25 May 2016
SRR1780516_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780516_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13875
Sequences flagged as poor quality0
Sequence length76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA150.002178657670.01
TGCACGA200.006808627452.528
CACAATA200.006808627452.569
CTCTTTT200.006808627452.541
CACGAAG200.006808627452.530
CCCTGCC200.006808627452.513
ATGCACG200.006808627452.527
GTGGTCG200.006808627452.545
GGTCGCC200.006808627452.547
TGCCGAC952.5001282E-729.47368616
CGCTGCC1201.9099389E-929.16666613
GCCGACG853.6011825E-628.8235317
GCTGCCG1056.002956E-726.66666814
CTGCCGA1056.002956E-726.66666815
ACGCTGC1201.923605E-623.33333212
CCGACGA901.7984866E-423.33333218
GACGCTG1102.6638843E-522.27272611
ATACACA2054.5474735E-1122.1951221
CTGACGC1355.349528E-620.740749
TGACGCT1205.2136304E-520.41666610
Produced by FastQC (version 0.11.5)