##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780516_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13875 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.152 21.0 12.0 31.0 12.0 34.0 2 19.66299099099099 21.0 11.0 31.0 11.0 34.0 3 20.441297297297297 21.0 11.0 31.0 11.0 34.0 4 21.66356756756757 23.0 11.0 32.0 11.0 34.0 5 21.629837837837837 23.0 11.0 32.0 11.0 34.0 6 21.505657657657657 21.0 11.0 34.0 11.0 37.0 7 23.13174774774775 25.0 11.0 34.0 11.0 37.0 8 21.66717117117117 21.0 11.0 33.0 11.0 37.0 9 22.74227027027027 22.0 12.0 34.0 10.0 37.0 10-11 22.049765765765766 21.0 11.0 34.0 11.0 37.0 12-13 22.068684684684683 21.5 11.0 34.0 11.0 37.0 14-15 21.788792792792794 21.0 11.0 33.5 11.0 37.0 16-17 21.47246846846847 21.0 11.0 32.5 11.0 37.0 18-19 21.83790990990991 21.5 11.0 33.5 11.0 37.0 20-21 21.883747747747748 22.0 11.0 32.5 11.0 37.0 22-23 21.304648648648648 21.0 11.0 32.0 11.0 37.0 24-25 21.502522522522522 21.0 11.0 32.0 10.5 37.0 26-27 21.144936936936936 21.0 11.0 31.0 10.5 37.0 28-29 20.732108108108108 21.0 11.0 31.0 11.0 37.0 30-31 20.729513513513513 21.0 11.0 31.0 11.0 37.0 32-33 20.976144144144143 21.0 11.0 31.0 11.0 37.0 34-35 21.241909909909907 21.0 11.0 31.0 11.0 37.0 36-37 20.96998198198198 21.0 11.0 31.0 11.0 37.0 38-39 20.37473873873874 21.0 11.0 29.5 11.0 37.0 40-41 20.66789189189189 21.0 11.0 29.0 11.0 36.5 42-43 20.956288288288288 21.0 11.0 31.0 11.0 37.0 44-45 20.599351351351352 21.0 11.0 31.0 11.0 36.0 46-47 20.865945945945946 21.0 11.0 31.0 11.0 37.0 48-49 21.051567567567567 21.0 11.0 31.0 11.0 37.0 50-51 21.04673873873874 21.0 11.0 31.0 11.0 37.0 52-53 21.049981981981983 21.0 11.0 31.0 11.0 37.0 54-55 21.06518918918919 21.0 11.0 31.0 11.0 37.0 56-57 20.581333333333333 20.5 11.0 31.0 11.0 36.5 58-59 20.61009009009009 20.5 11.0 30.5 11.0 36.5 60-61 20.7898018018018 21.0 11.0 31.0 11.0 36.0 62-63 20.45607207207207 19.5 11.0 30.5 11.0 36.0 64-65 20.636504504504504 20.0 11.0 30.5 11.0 36.0 66-67 20.80947747747748 20.5 11.0 31.0 11.0 36.5 68-69 20.058234234234234 19.0 11.0 28.0 11.0 36.0 70-71 19.853405405405404 19.0 11.0 27.0 11.0 36.0 72-73 19.62291891891892 19.0 11.0 27.0 11.0 36.0 74-75 19.957081081081082 19.0 11.0 27.0 11.0 36.0 76 19.27300900900901 19.0 11.0 25.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4303558965515428 1101 2 1.183479785289613 1101 3 0.22458557546388036 1101 4 0.09721635319680288 1101 5 -0.43643185943358986 1101 6 0.0025729843327546575 1101 7 -1.3102763108639657 1101 8 -3.843129833707504 1101 9 1.1485397838649618 1101 10-11 -1.2058425180556647 1101 12-13 1.1896098836308369 1101 14-15 1.3057467961316362 1101 16-17 -0.2607828611111742 1101 18-19 0.04893361536995755 1101 20-21 -0.5826070979503086 1101 22-23 1.9321124959547795 1101 24-25 -2.1796559053218587 1101 26-27 -0.06693820664359151 1101 28-29 1.0384298135809296 1101 30-31 -1.0304950109597542 1101 32-33 -1.2569259499794896 1101 34-35 -2.588460380614361 1101 36-37 0.10377597449560483 1101 38-39 0.9667937595804155 1101 40-41 1.2294862532494015 1101 42-43 -1.4952683646724125 1101 44-45 -1.5065707978515093 1101 46-47 0.3077366253712164 1101 48-49 -3.7776420232462407 1101 50-51 -3.3977268635034044 1101 52-53 -0.3961536733135844 1101 54-55 -0.7810441877684617 1101 56-57 -2.7755965209559292 1101 58-59 0.09976533159094103 1101 60-61 -1.8987536273227654 1101 62-63 -3.1841837872403644 1101 64-65 -3.3998334786843962 1101 66-67 -2.8280801277728003 1101 68-69 -1.6378881141855253 1101 70-71 -1.1191141309141344 1101 72-73 -2.1869131621507023 1101 74-75 -1.1545533739424556 1101 76 -0.9228800695631421 1102 1 -1.3120683458726994 1102 2 0.8834797852896159 1102 3 1.1215552724335787 1102 4 1.4911557471361974 1102 5 2.6272045042027727 1102 6 0.7662093479691165 1102 7 1.2715418709542163 1102 8 2.053839863262194 1102 9 0.41823675356193135 1102 10-11 -0.6785697907829373 1102 12-13 -1.2588749648540087 1102 14-15 2.2224134627983005 1102 16-17 -0.12290407323238739 1102 18-19 -0.2874300209936784 1102 20-21 0.9461807808375688 1102 22-23 3.16241552625781 1102 24-25 1.9339804583145082 1102 26-27 0.5966981569927725 1102 28-29 2.771763146914264 1102 30-31 2.3392019587372133 1102 32-33 -0.4538956469491886 1102 34-35 2.6721456799917007 1102 36-37 2.181048701768333 1102 38-39 2.4016422444289027 1102 40-41 3.3128195865827372 1102 42-43 1.9077619383578899 1102 44-45 -0.24899504027575148 1102 46-47 2.130463898098487 1102 48-49 2.657206461602243 1102 50-51 0.279545863769318 1102 52-53 -0.28251730967721755 1102 54-55 0.41744066071639097 1102 56-57 1.971373176013767 1102 58-59 0.7740077558333702 1102 60-61 0.7254887969196595 1102 62-63 0.3855131824566058 1102 64-65 1.2804695516186335 1102 66-67 1.4370713873787153 1102 68-69 -0.23940326570067683 1102 70-71 1.6839161721161702 1102 72-73 2.1312686560311143 1102 74-75 -0.4621291315182141 1102 76 1.2771199304368572 1103 1 -1.411749366606351 1103 2 0.16418153967557814 1103 3 1.4158136456393215 1103 4 -1.7010292608382862 1103 5 0.2635681405664094 1103 6 1.0867835106485444 1103 7 2.616039478609718 1103 8 1.6287999908538993 1103 9 1.562574871584257 1103 10-11 1.4932802889618806 1103 12-13 1.8045221643325888 1103 14-15 1.1171503049035607 1103 16-17 2.361146963450228 1103 18-19 1.6515651943173282 1103 20-21 0.7472174634532003 1103 22-23 2.1277265310425015 1103 24-25 0.17999321748516195 1103 26-27 0.6585003898476387 1103 28-29 0.9822894627037364 1103 30-31 1.7642418311455046 1103 32-33 0.23430212019594876 1103 34-35 1.4273290930698508 1103 36-37 0.3441268516885856 1103 38-39 -0.4165395737529174 1103 40-41 0.5365037971090523 1103 42-43 0.3196439160293423 1103 44-45 -1.6907813241672969 1103 46-47 -0.2168247781375534 1103 48-49 0.7293755206134058 1103 50-51 1.6092906803562421 1103 52-53 1.5117410635285182 1103 54-55 -1.4836757667158302 1103 56-57 -1.6685789770962813 1103 58-59 0.2620460333453316 1103 60-61 2.0249305832035525 1103 62-63 0.25792147591753434 1103 64-65 -1.188429969912466 1103 66-67 -0.17895732075525217 1103 68-69 -0.7326249562907883 1103 70-71 1.0124648164542869 1103 72-73 0.7078236799545579 1103 74-75 -0.23086916341614128 1103 76 -1.056213402896475 1104 1 1.4414670076626557 1104 2 1.3945908964007252 1104 3 0.8912522421305482 1104 4 -2.3472280912476435 1104 5 -0.8253207483224791 1104 6 -0.6640936823339132 1104 7 0.26750146691381005 1104 8 -0.2653520559297249 1104 9 1.3596508949760704 1104 10-11 -0.06139807361121896 1104 12-13 0.6340543280752833 1104 14-15 -0.9886976483128116 1104 16-17 -0.8107828611111749 1104 18-19 -2.039955273518931 1104 20-21 -2.721495986839198 1104 22-23 -0.7012208373785533 1104 24-25 -2.5074336830996344 1104 26-27 -2.0224937621991472 1104 28-29 -2.0726812975301776 1104 30-31 -0.3304950109597584 1104 32-33 -2.2791481722017117 1104 34-35 -1.4773492695032502 1104 36-37 -1.624001803282173 1104 38-39 -1.2720951293084717 1104 40-41 0.01281958658273652 1104 42-43 -0.9619350313390775 1104 44-45 -1.2065707978515086 1104 46-47 -2.342263374628782 1104 48-49 -1.8498642454684635 1104 50-51 -1.303282419058963 1104 52-53 -1.6017092288691401 1104 54-55 -0.6865997433240167 1104 56-57 -0.32559652095592995 1104 58-59 -0.5613457795201668 1104 60-61 -0.23208696065609402 1104 62-63 -2.0175171205736966 1104 64-65 -1.1609445897955055 1104 66-67 -1.9114134611061289 1104 68-69 -2.2767770030744146 1104 70-71 -1.6524474642474658 1104 72-73 -0.6980242732618152 1104 74-75 -0.6378867072757899 1104 76 -0.8784356251186978 1105 1 1.0233791523654965 1105 2 -0.6203961837026348 1105 3 1.0369886762390763 1105 4 -1.9415433367256796 1105 5 -0.15503651059638202 1105 6 -1.7369618993881772 1105 7 -0.23585770621280489 1105 8 0.41888567016846423 1105 9 -1.6289795959800024 1105 10-11 -1.6655324405362855 1105 12-13 1.6671292634758004 1105 14-15 0.5131111372169066 1105 16-17 -1.2208603804910183 1105 18-19 -0.8487408032346941 1105 20-21 0.45498980127449684 1105 22-23 -1.7593603722622753 1105 24-25 -1.4800435022210792 1105 26-27 -0.7359304547056098 1105 28-29 -1.3212601088996898 1105 30-31 -2.3514252435178946 1105 32-33 0.3089655228887267 1105 34-35 0.6464233403158701 1105 36-37 -0.588859684419127 1105 38-39 -1.3118884109622222 1105 40-41 0.16165679588506165 1105 42-43 -0.282865263897218 1105 44-45 -1.983314983898019 1105 46-47 -0.09962771571405327 1105 48-49 0.7963889845056933 1105 50-51 1.4902576326206223 1105 52-53 -1.285688557034515 1105 54-55 -1.3380209319545067 1105 56-57 -1.0666817922737586 1105 58-59 -0.9099245908896769 1105 60-61 -0.7568931622064845 1105 62-63 -1.1655791360775716 1105 64-65 0.13776342054040924 1105 66-67 0.7319973916070452 1105 68-69 -2.4018416025576173 1105 70-71 -1.1772536657978563 1105 72-73 -1.1171457202902388 1105 74-75 -1.6115301181285027 1105 76 -0.846911077315081 1106 1 0.6483046145002618 1106 2 -2.4242125224026942 1106 3 0.11689326777157305 1106 4 -0.005347749367299315 1106 5 -0.4800216030233351 1106 6 -1.1102475284877613 1106 7 0.5769031763155184 1106 8 0.792767602189933 1106 9 -0.551460216135041 1106 10-11 -0.40584251805566396 1106 12-13 -0.17962088559993106 1106 14-15 -0.6878429474581083 1106 16-17 -1.038987989316304 1106 18-19 -0.03568176924542854 1106 20-21 0.1699570046137957 1106 22-23 0.8500612139034978 1106 24-25 0.890856915190966 1106 26-27 -0.1643741040794886 1106 28-29 -1.8820830069318895 1106 30-31 -0.040751421216164374 1106 32-33 -0.19282338587692394 1106 34-35 -0.31153730369128496 1106 36-37 -0.8026342819146528 1106 38-39 -2.621667778881122 1106 40-41 0.40256317632632843 1106 42-43 -0.2414222108262578 1106 44-45 2.1600958688151586 1106 46-47 0.06286483049942149 1106 48-49 -0.6353343309385515 1106 50-51 -0.29388070965725177 1106 52-53 0.3153847882248755 1106 54-55 -0.290018546742818 1106 56-57 -0.9179042132636219 1106 58-59 -1.727157745332132 1106 60-61 -1.2243946529637881 1106 62-63 0.14658544352887048 1106 64-65 -2.0447052735561897 1106 66-67 -1.1165416662343333 1106 68-69 -0.8955804218778312 1106 70-71 -0.9780884898884921 1106 72-73 -1.1228105980481402 1106 74-75 0.42365175426267143 1106 76 -0.38954673622980707 1107 1 1.0859114521070978 1107 2 -1.1609646591548284 1107 3 -0.15319220231389608 1107 4 -0.036116980136529975 1107 5 0.01912369612196585 1107 6 0.35812853988830895 1107 7 0.26750146691381005 1107 8 -0.44312983370750203 1107 9 -0.9959046605794839 1107 10-11 -0.6391758513889982 1107 12-13 0.25627655029750684 1107 14-15 0.37796901835385555 1107 16-17 -0.3996717500000635 1107 18-19 -1.4510663846300424 1107 20-21 -0.06594043128364291 1107 22-23 0.7765569403992245 1107 24-25 0.7036774280114741 1107 26-27 -0.47804931775470294 1107 28-29 -0.35045907530795617 1107 30-31 0.5361716557069123 1107 32-33 -0.3458148388683817 1107 34-35 -1.588460380614361 1107 36-37 0.4871093078289377 1107 38-39 0.5723493151359698 1107 40-41 -0.6982915245283756 1107 42-43 0.4713983019942525 1107 44-45 0.44898475770404644 1107 46-47 -1.4200411524065615 1107 48-49 -0.5165309121351314 1107 50-51 -0.38106019683673864 1107 52-53 -0.3128203399802487 1107 54-55 -0.508821965546236 1107 56-57 0.10773681237740362 1107 58-59 0.5608764427020567 1107 60-61 0.6012463726772346 1107 62-63 -0.1286282316848073 1107 64-65 0.40572207687115736 1107 66-67 -0.4891912388839117 1107 68-69 0.6676674413700319 1107 70-71 -0.3635585753585815 1107 72-73 0.4575312822937434 1107 74-75 -0.8823311517202335 1107 76 0.34378659710352366 1108 1 -1.0952851290894827 1108 2 -3.065238163428333 1108 3 0.014329165207470851 1108 4 0.045934301914751785 1108 5 -2.608226731228463 1108 6 -2.135888554128787 1108 7 -0.4487378493255072 1108 8 -2.0533862439639137 1108 9 -0.038639703314526486 1108 10-11 -1.5981502103633574 1108 12-13 -1.9232106291896756 1108 14-15 -1.9186121782273418 1108 16-17 -0.0774495277778442 1108 18-19 -0.5741433077069651 1108 20-21 -1.1377353030785144 1108 22-23 -1.7012208373785533 1108 24-25 -0.41683539250134416 1108 26-27 -0.3053997451051309 1108 28-29 -1.7410573659062472 1108 30-31 -1.8612642417289855 1108 32-33 -0.2569259499794896 1108 34-35 0.6115396193856384 1108 36-37 0.5178785385981683 1108 38-39 -0.8524370096503517 1108 40-41 -1.1615393877762372 1108 42-43 0.7201162507122056 1108 44-45 0.8267625354818264 1108 46-47 -1.2704685028339107 1108 48-49 1.326204130599912 1108 50-51 0.013811598035054828 1108 52-53 -2.2615382886981976 1108 54-55 -0.3413005980248691 1108 56-57 -0.9948272901866986 1108 58-59 -0.12459364276802987 1108 60-61 -0.5064459350150692 1108 62-63 0.3132521101955348 1108 64-65 -0.9293206581715765 1108 66-67 -1.5267980764907492 1108 68-69 -1.280195806493218 1108 70-71 -1.5806525924525943 1108 72-73 0.5310355557980166 1108 74-75 -0.781476450865533 1108 76 -3.7485210952041665 1109 1 -1.2159855668630897 1109 2 1.14364238691563 1109 3 -0.5347640180320532 1109 4 2.357378954822817 1109 5 1.8977144820298228 1109 6 -0.6462075034721231 1109 7 1.4555773476726195 1109 8 0.6820734183250181 1109 9 -0.17747647629764174 1109 10-11 0.049442034789866085 1109 12-13 1.5205041925739273 1109 14-15 0.6736329749934242 1109 16-17 -0.3172869261518265 1109 18-19 -1.0778956529227237 1109 20-21 -1.4976477483568118 1109 22-23 -0.21341595932977242 1109 24-25 -1.1938835475982792 1109 26-27 -0.0067756050175731275 1109 28-29 0.6863972932557267 1109 30-31 -0.6061047670573174 1109 32-33 -0.789446275182744 1109 34-35 0.4042225462149034 1109 36-37 -1.745411017374316 1109 38-39 0.9371189628324466 1109 40-41 1.495746415851027 1109 42-43 0.9258698467096984 1109 44-45 -0.4090098222417531 1109 46-47 1.581313861143574 1109 48-49 -0.25040625088852053 1109 50-51 0.16609427470797655 1109 52-53 1.1709194974181258 1109 54-55 0.5933460561339778 1109 56-57 1.7540782757920397 1109 58-59 0.7074889088267149 1109 60-61 0.7524658848723611 1109 62-63 0.842645481052319 1109 64-65 -0.2051180315299277 1109 66-67 0.5601312543410231 1109 68-69 0.5340631053266698 1109 70-71 0.3321053812809893 1109 72-73 0.15455025248344612 1109 74-75 1.6003246748380313 1109 76 0.18768903612791377 1110 1 -1.8696441034484579 1110 2 -0.49747259566276725 1110 3 1.4150617659400737 1110 4 -1.626593170612722 1110 5 -2.1078604308621607 1110 6 0.631144412904181 1110 7 -0.5959905965782539 1110 8 0.509251118673447 1110 9 -0.7133649780398024 1110 10-11 -0.31536632757947203 1110 12-13 -1.7913424973215442 1110 14-15 -0.2918722514874119 1110 16-17 -1.4441161944445078 1110 18-19 -1.1653520989157542 1110 20-21 0.10548814014493146 1110 22-23 -0.9440779802356971 1110 24-25 -1.5820368577028106 1110 26-27 -0.2907477304531163 1110 28-29 -0.22823685308573616 1110 30-31 -1.9304950109597563 1110 32-33 0.647835954782412 1110 34-35 0.025825333671349426 1110 36-37 -0.20812878740915863 1110 38-39 -2.0736824308957758 1110 40-41 -0.8871804134172656 1110 42-43 -0.8905064599105081 1110 44-45 -2.30657079785151 1110 46-47 -3.8089300412954508 1110 48-49 0.5199770243728032 1110 50-51 -0.6001078158843605 1110 52-53 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-1.0522563089805814 2116 56-57 -0.08923288459229184 2116 58-59 -0.7411437593181454 2116 60-61 -0.9260263545954892 2116 62-63 0.6885434854869104 2116 64-65 0.5986513698004501 2116 66-67 0.2855562358635666 2116 68-69 -0.34546387176128235 2116 70-71 -0.346386858186861 2116 72-73 -1.03539801063555 2116 74-75 0.7651435957545125 2116 76 1.3377259910429196 2117 1 2.6273255935212383 2117 2 1.9440858458956747 2117 3 0.9094340603123676 2117 4 1.1881254441058928 2117 5 0.990840867839136 2117 6 5.099542681302449 2117 7 1.7563903558027008 2117 8 1.4780822875046162 2117 9 1.9333882687134434 2117 10-11 0.3365817243685747 2117 12-13 0.1199129139338666 2117 14-15 0.813322553707394 2117 16-17 -1.0319949823232974 2117 18-19 1.8640851305214738 2117 20-21 0.6583019929587799 2117 22-23 2.783627647469931 2117 24-25 2.8885259128599614 2117 26-27 1.9603345206291358 2117 28-29 2.302066177217295 2117 30-31 1.0816262011614555 2117 32-33 -0.3781380711916107 2117 34-35 3.0812365890826072 2117 36-37 0.7416547623743917 2117 38-39 0.9319452747319303 2117 40-41 1.0552438290069794 2117 42-43 2.968367998963952 2117 44-45 1.3116110203303109 2117 46-47 3.160766928401518 2117 48-49 0.05114585554163753 2117 50-51 0.15833374255720045 2117 52-53 2.7932402660803533 2117 54-55 2.265925509201235 2117 56-57 2.6531913578319504 2117 58-59 1.2588562406818546 2117 60-61 1.2860948575257218 2117 62-63 2.4006646976081214 2117 64-65 1.0228937940428793 2117 66-67 0.5128289631362932 2117 68-69 2.9121118858144754 2117 70-71 2.0324010206010144 2117 72-73 3.05551108027354 2117 74-75 2.8712042018151216 2117 76 1.7013623546792829 2118 1 1.5874987536944012 2118 2 2.5596702614800897 2118 3 -0.6420810912027832 2118 4 1.9734068293872795 2118 5 -1.4507175737193059 2118 6 -0.11171272995296277 2118 7 0.461152260564603 2118 8 -0.23360602418369325 2118 9 0.2294921648173407 2118 10-11 2.156062243849096 2118 12-13 0.4086575026784587 2118 14-15 -1.20615796577313 2118 16-17 1.9415980912697783 2118 18-19 1.3203621867985298 2118 20-21 3.5912024258592155 2118 22-23 0.8273505911928751 2118 24-25 1.5322488565829069 2118 26-27 0.09496655526116982 2118 28-29 0.65747743262855 2118 30-31 0.7409335604688181 2118 32-33 2.662121669068128 2118 34-35 -0.002746094900075491 2118 36-37 1.077585498305126 2118 38-39 0.08346042624708261 2118 40-41 -0.2871804134172642 2118 42-43 -0.6190778884819359 2118 44-45 0.6791434878627776 2118 46-47 2.1339271015616923 2118 48-49 0.1485484529442367 2118 50-51 -0.9143935301700736 2118 52-53 -0.8032965304564428 2118 54-55 -0.2596156163398895 2118 56-57 0.5648796695202591 2118 58-59 -1.0311870493614386 2118 60-61 -0.6558964844656181 2118 62-63 0.4015304984739245 2118 64-65 1.031118902267984 2118 66-67 2.098110348417677 2118 68-69 -0.3593166856140968 2118 70-71 0.4380287262287226 2118 72-73 -0.3726274478649856 2118 74-75 1.6954466260575423 2118 76 3.0866437399606674 2119 1 1.4118373780330238 2119 2 0.32792422973405877 2119 3 0.350511501389807 2119 4 1.8009200569005053 2119 5 1.4857903627886309 2119 6 -0.48631590455613605 2119 7 2.423057022469365 2119 8 1.3568701662924951 2119 9 -0.32923799391281605 2119 10-11 0.6867500745369277 2119 12-13 0.6081284021493545 2119 14-15 3.1298208702057053 2119 16-17 0.8299578796295677 2119 18-19 -0.4658811994448584 2119 20-21 -0.48816265350586363 2119 22-23 -0.03455417071188549 2119 24-25 2.1999737243077746 2119 26-27 3.1034321637267794 2119 28-29 1.9754668506179662 2119 30-31 -0.6675320479967937 2119 32-33 0.8356666426131021 2119 34-35 -0.3329048250588045 2119 36-37 0.8797019004215301 2119 38-39 1.787164129950785 2119 40-41 0.42393069769384795 2119 42-43 0.419546450142402 2119 44-45 0.6786143873336741 2119 46-47 1.005884773519366 2119 48-49 0.17235797675375863 2119 50-51 0.348569432792889 2119 52-53 1.2427352155753049 2119 54-55 -0.9125256692499413 2119 56-57 -1.0218928172522261 2119 58-59 0.1275431093687196 2119 60-61 1.041987113417978 2119 62-63 0.48989028683371316 2119 64-65 0.8797961509452321 2119 66-67 0.8478457981531307 2119 68-69 1.3380378117403993 2119 70-71 2.269774757974755 2119 72-73 1.498272023034481 2119 74-75 0.8324836630945818 2119 76 2.610453263770193 >>END_MODULE >>Per sequence quality scores fail #Quality Count 11 1.0 12 37.0 13 250.0 14 705.0 15 1030.0 16 1261.0 17 1455.0 18 1590.0 19 1540.0 20 1251.0 21 977.0 22 654.0 23 515.0 24 360.0 25 238.0 26 177.0 27 141.0 28 107.0 29 98.0 30 80.0 31 116.0 32 98.0 33 110.0 34 166.0 35 202.0 36 329.0 37 387.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.321550410103795 26.80554547434129 27.814473397691806 35.058430717863104 2 18.7027027027027 22.637837837837836 33.354954954954955 25.304504504504504 3 6.212612612612613 27.387387387387385 41.001801801801804 25.398198198198195 4 4.655855855855856 25.376576576576575 25.05225225225225 44.91531531531531 5 3.9783783783783786 30.933333333333334 21.686486486486487 43.4018018018018 6 19.81981981981982 29.96036036036036 13.052252252252252 37.167567567567566 7 7.25045045045045 32.9009009009009 12.021621621621621 47.82702702702702 8 15.236036036036035 33.945945945945944 14.234234234234233 36.58378378378379 9 14.277477477477477 26.91171171171171 12.18018018018018 46.630630630630634 10-11 9.859459459459458 28.464864864864865 25.08108108108108 36.59459459459459 12-13 9.041441441441442 26.02162162162162 24.475675675675674 40.461261261261264 14-15 13.311711711711713 30.30990990990991 19.945945945945944 36.432432432432435 16-17 17.145945945945947 29.664864864864864 19.344144144144142 33.84504504504505 18-19 12.839639639639639 27.589189189189188 18.317117117117117 41.25405405405406 20-21 11.091891891891892 28.89009009009009 19.506306306306307 40.51171171171171 22-23 18.036036036036034 28.630630630630634 16.313513513513513 37.01981981981982 24-25 14.248648648648649 34.306306306306304 15.354954954954955 36.09009009009009 26-27 11.881081081081081 34.33513513513514 20.933333333333334 32.85045045045045 28-29 15.423979242495225 29.193844823236876 24.072939565389746 31.309236368878157 30-31 14.558558558558559 29.64684684684685 25.279279279279283 30.515315315315316 32-33 10.86126126126126 35.67207207207207 22.425225225225226 31.041441441441442 34-35 10.962162162162162 33.7009009009009 21.502702702702702 33.83423423423423 36-37 12.057657657657659 29.53873873873874 24.72072072072072 33.68288288288288 38-39 12.23063063063063 25.34054054054054 27.790990990990995 34.637837837837836 40-41 10.223423423423423 27.592792792792793 25.308108108108108 36.87567567567567 42-43 6.879279279279279 25.74054054054054 29.027027027027025 38.35315315315315 44-45 8.100900900900902 28.284684684684684 29.783783783783786 33.83063063063063 46-47 8.612612612612612 31.18198198198198 25.31171171171171 34.89369369369369 48-49 8.659459459459459 33.61441441441441 21.185585585585585 36.54054054054054 50-51 8.807207207207208 31.308108108108108 20.08648648648649 39.7981981981982 52-53 7.783783783783783 30.443243243243245 22.2990990990991 39.47387387387387 54-55 8.201801801801802 30.792792792792795 20.673873873873873 40.33153153153153 56-57 11.603603603603602 31.041441441441442 19.131531531531532 38.22342342342343 58-59 10.677477477477478 29.88828828828829 18.954954954954957 40.47927927927928 60-61 8.252252252252253 28.00720720720721 20.36756756756757 43.372972972972974 62-63 10.126126126126128 29.89189189189189 18.51891891891892 41.46306306306306 64-65 10.486486486486486 31.27207207207207 16.01081081081081 42.230630630630635 66-67 9.102702702702704 36.01441441441441 14.666666666666666 40.21621621621622 68-69 13.077477477477478 35.25045045045045 14.872072072072074 36.8 70-71 13.581981981981983 35.07027027027027 15.693693693693694 35.65405405405405 72-73 13.917117117117117 35.852252252252256 15.246846846846848 34.983783783783785 74-75 11.744144144144144 36.87927927927928 14.868468468468468 36.508108108108104 76 10.616216216216216 33.93153153153153 17.52072072072072 37.93153153153153 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.5 12 2.0 13 1.5 14 1.0 15 3.0 16 5.0 17 5.0 18 6.0 19 10.0 20 12.5 21 12.0 22 20.0 23 27.5 24 31.0 25 35.0 26 42.0 27 50.5 28 57.5 29 63.0 30 70.5 31 91.5 32 124.5 33 144.0 34 155.0 35 222.5 36 286.5 37 294.0 38 361.0 39 450.0 40 527.0 41 599.5 42 617.0 43 685.0 44 785.0 45 832.5 46 848.0 47 869.5 48 887.5 49 847.5 50 811.0 51 817.0 52 772.5 53 688.0 54 654.0 55 634.5 56 566.0 57 467.5 58 418.0 59 394.0 60 342.5 61 276.0 62 237.0 63 204.5 64 159.5 65 127.5 66 88.5 67 69.0 68 54.5 69 47.0 70 42.5 71 31.0 72 26.0 73 17.5 74 14.0 75 14.0 76 12.0 77 7.5 78 6.0 79 7.0 80 6.0 81 5.0 82 5.5 83 6.0 84 4.5 85 3.5 86 4.0 87 4.0 88 2.0 89 1.5 90 2.5 91 3.5 92 5.0 93 3.0 94 1.5 95 1.5 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7063063063063063 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0036036036036036037 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 13875.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.44864864864866 #Duplication Level Percentage of deduplicated Percentage of total 1 98.15842023518971 95.65405405405404 2 1.2351157458767843 2.407207207207207 3 0.48812957621477704 1.4270270270270269 4 0.08875083203905038 0.34594594594594597 5 0.014791805339841728 0.07207207207207207 6 0.007395902669920864 0.043243243243243246 7 0.007395902669920864 0.05045045045045045 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.021621621621621623 0.0 2 0.0 0.0 0.0 0.02882882882882883 0.0 3 0.0 0.0 0.0 0.02882882882882883 0.0 4 0.0 0.0 0.0 0.02882882882882883 0.0 5 0.0 0.0 0.0 0.02882882882882883 0.0 6 0.0 0.0 0.0 0.02882882882882883 0.0 7 0.0 0.0 0.0 0.02882882882882883 0.0 8 0.0 0.0 0.0 0.02882882882882883 0.0 9 0.0 0.0 0.0 0.02882882882882883 0.0 10 0.0 0.0 0.0 0.02882882882882883 0.0 11 0.0 0.0 0.0 0.02882882882882883 0.0 12 0.0 0.0 0.0 0.02882882882882883 0.0 13 0.0 0.0 0.0 0.02882882882882883 0.0 14 0.0 0.0 0.0 0.02882882882882883 0.0 15 0.0 0.0 0.0 0.02882882882882883 0.0 16 0.0 0.0 0.0 0.02882882882882883 0.0 17 0.0 0.0 0.0 0.02882882882882883 0.0 18 0.0 0.0 0.0 0.02882882882882883 0.0 19 0.0 0.0 0.0 0.05765765765765766 0.0 20 0.0 0.0 0.0 0.05765765765765766 0.0 21 0.0 0.0 0.0 0.06486486486486487 0.0 22 0.0 0.0 0.0 0.06486486486486487 0.0 23 0.0 0.0 0.0 0.06486486486486487 0.0 24 0.0 0.0 0.0 0.06486486486486487 0.0 25 0.0 0.0 0.0 0.06486486486486487 0.0 26 0.0 0.0 0.0 0.06486486486486487 0.0 27 0.0 0.0 0.0 0.06486486486486487 0.0 28 0.0 0.0 0.0 0.06486486486486487 0.0 29 0.0 0.0 0.0 0.06486486486486487 0.0 30 0.0 0.0 0.0 0.0936936936936937 0.0 31 0.0 0.0 0.0 0.12972972972972974 0.0 32 0.0 0.0 0.0 0.12972972972972974 0.0 33 0.0 0.0 0.0 0.15135135135135136 0.0 34 0.0 0.0 0.0 0.1945945945945946 0.0 35 0.0 0.0 0.0 0.22342342342342342 0.0 36 0.0 0.0 0.0 0.2594594594594595 0.0 37 0.0 0.0 0.0 0.35315315315315315 0.0 38 0.0 0.0 0.0 0.5117117117117117 0.0 39 0.0 0.0 0.0 0.6630630630630631 0.0 40 0.0 0.0 0.0 0.8072072072072072 0.0 41 0.0 0.0 0.0 0.9585585585585585 0.0 42 0.0 0.0 0.0 1.0810810810810811 0.0 43 0.0 0.0 0.0 1.2756756756756757 0.0 44 0.0 0.0 0.0 1.4414414414414414 0.0 45 0.0 0.0 0.0 1.6144144144144144 0.0 46 0.0 0.0 0.0 1.7585585585585586 0.0 47 0.0 0.0 0.0 1.8954954954954955 0.0 48 0.0 0.0 0.0 2.0324324324324325 0.0 49 0.0 0.0 0.0 2.1621621621621623 0.0 50 0.0 0.0 0.0 2.27027027027027 0.0 51 0.0 0.0 0.0 2.4 0.0 52 0.0 0.0 0.0 2.4936936936936935 0.0 53 0.0 0.0 0.0 2.5585585585585586 0.0 54 0.0 0.0 0.0 2.7027027027027026 0.0 55 0.0 0.0 0.0 2.7675675675675677 0.0 56 0.0 0.0 0.0 2.8468468468468466 0.0 57 0.0 0.0 0.0 2.926126126126126 0.0 58 0.0 0.0 0.0 3.099099099099099 0.0 59 0.0 0.0 0.0 3.156756756756757 0.0 60 0.0 0.0 0.0 3.272072072072072 0.0 61 0.0 0.0 0.0 3.3513513513513513 0.0 62 0.0 0.0 0.0 3.4018018018018017 0.0 63 0.0 0.0 0.0 3.5315315315315314 0.0 64 0.0 0.0 0.0 3.618018018018018 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTACA 15 0.0021786576 70.0 1 TGCACGA 20 0.0068086274 52.5 28 CACAATA 20 0.0068086274 52.5 69 CTCTTTT 20 0.0068086274 52.5 41 CACGAAG 20 0.0068086274 52.5 30 CCCTGCC 20 0.0068086274 52.5 13 ATGCACG 20 0.0068086274 52.5 27 GTGGTCG 20 0.0068086274 52.5 45 GGTCGCC 20 0.0068086274 52.5 47 TGCCGAC 95 2.5001282E-7 29.473686 16 CGCTGCC 120 1.9099389E-9 29.166666 13 GCCGACG 85 3.6011825E-6 28.82353 17 GCTGCCG 105 6.002956E-7 26.666668 14 CTGCCGA 105 6.002956E-7 26.666668 15 ACGCTGC 120 1.923605E-6 23.333332 12 CCGACGA 90 1.7984866E-4 23.333332 18 GACGCTG 110 2.6638843E-5 22.272726 11 ATACACA 205 4.5474735E-11 22.195122 1 CTGACGC 135 5.349528E-6 20.74074 9 TGACGCT 120 5.2136304E-5 20.416666 10 >>END_MODULE