FastQCFastQC Report
Wed 25 May 2016
SRR1780516_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780516_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13875
Sequences flagged as poor quality0
Sequence length76
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT291220.987387387387386No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT254618.34954954954955RNA PCR Primer, Index 15 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC8165.881081081081081No Hit
CTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC4283.0846846846846847No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG3272.3567567567567567No Hit
TATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT2421.744144144144144No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC1441.037837837837838No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGT1220.8792792792792792No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC1210.872072072072072No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG1110.8No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTCTT890.6414414414414414No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGT830.5981981981981982No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC810.5837837837837837No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTCTT760.5477477477477477No Hit
CTTATAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG730.5261261261261262No Hit
CTTAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC690.4972972972972973No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTCTT670.4828828828828829No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGT670.4828828828828829No Hit
TTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC650.46846846846846846No Hit
CTTATTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG590.4252252252252252No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG440.3171171171171171No Hit
CTTATCACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC360.2594594594594595No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGT310.22342342342342342No Hit
CTTATACACATCTCCGAGCCCACGCGACCGCGGACAATCTCGTATGCCGT280.2018018018018018No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCG280.2018018018018018No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCGGACAATCTCGTATGCCGT250.18018018018018017No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGT230.16576576576576577No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTAGTATGCCGT220.15855855855855855No Hit
ACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCT220.15855855855855855RNA PCR Primer, Index 15 (95% over 23bp)
CTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT210.15135135135135136No Hit
TCTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT210.15135135135135136No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTCTGCCGTCTT210.15135135135135136No Hit
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTC200.14414414414414414No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCAGTCTT190.13693693693693693No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGTCTT190.13693693693693693Illumina PCR Primer Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCAGT190.13693693693693693No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG180.12972972972972974No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGTCTT180.12972972972972974Illumina PCR Primer Index 4 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCGCGGACACTCTCGTATGCCGTCTT180.12972972972972974TruSeq Adapter, Index 19 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTC180.12972972972972974No Hit
TCTTAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT170.12252252252252253No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAAATCGTATGCCGT170.12252252252252253No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAGCTCGTATGCCGT170.12252252252252253No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCG170.12252252252252253No Hit
TCTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT160.11531531531531532No Hit
CTTATATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC140.1009009009009009No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTATT140.1009009009009009No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGACAATCTCGTATGCCGTCTT140.1009009009009009RNA PCR Primer, Index 19 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGACGTCTT140.1009009009009009No Hit
ATACACATCTCCGAGCCCACGAGACAGCGGACAATCTCGTATGCCGTCTT140.1009009009009009RNA PCR Primer, Index 36 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTCC150.002178657670.059
CCTATTT150.002178657670.038
TTTCCTC150.002178657670.061
CCAAATC150.002178657670.023
CAACCTT150.002178657670.055
TAAAAAA150.002178657670.061
CATCCCT150.002178657670.02
TTTTTTA150.002178657670.015
TATAACA150.002178657670.03
TTATTAC150.002178657670.02
TAGCTGT150.002178657670.044
TTTTCCT150.002178657670.060
TTTTTAA150.002178657670.016
AACCTTT150.002178657670.056
ACCAAAT150.002178657670.022
GGACAAA207.655769E-570.029
TCTACTA150.002178657670.08
CAACAAA150.002178657670.070
AACCTAT150.002178657670.036
TTATAAC150.002178657670.02
Produced by FastQC (version 0.11.5)