##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780516_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13875 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75740540540541 34.0 34.0 34.0 31.0 34.0 2 33.18745945945946 34.0 34.0 34.0 32.0 34.0 3 33.491747747747745 34.0 34.0 34.0 34.0 34.0 4 33.47099099099099 34.0 34.0 34.0 34.0 34.0 5 33.50565765765766 34.0 34.0 34.0 34.0 34.0 6 37.34659459459459 38.0 38.0 38.0 37.0 38.0 7 36.92122522522523 38.0 37.0 38.0 36.0 38.0 8 36.90486486486486 38.0 37.0 38.0 35.0 38.0 9 36.5470990990991 38.0 37.0 38.0 34.0 38.0 10-11 36.29841441441441 38.0 37.0 38.0 33.0 38.0 12-13 35.97675675675676 38.0 36.5 38.0 32.5 38.0 14-15 35.915243243243246 38.0 36.0 38.0 31.0 38.0 16-17 35.88832432432432 38.0 36.0 38.0 32.0 38.0 18-19 36.04147747747747 38.0 36.5 38.0 32.5 38.0 20-21 35.8889009009009 38.0 36.0 38.0 31.0 38.0 22-23 35.82407207207207 38.0 36.0 38.0 32.5 38.0 24-25 34.861153153153154 37.0 34.5 38.0 28.0 38.0 26-27 34.92201801801802 37.0 34.0 38.0 30.0 38.0 28-29 34.81650450450451 37.5 35.0 38.0 28.0 38.0 30-31 33.99863063063063 37.0 34.0 38.0 24.0 38.0 32-33 34.67218018018018 37.0 34.0 38.0 26.0 38.0 34-35 33.657873873873875 37.0 32.5 38.0 23.0 38.0 36-37 33.528036036036035 37.0 34.0 38.0 23.0 38.0 38-39 31.683243243243243 36.0 28.0 37.5 11.0 38.0 40-41 33.227135135135136 36.5 33.0 38.0 23.0 38.0 42-43 34.75747747747748 37.0 34.5 38.0 28.5 38.0 44-45 35.10108108108108 37.0 35.0 38.0 29.0 38.0 46-47 35.08911711711712 37.0 35.0 38.0 30.5 38.0 48-49 34.63344144144144 37.0 35.0 38.0 27.5 38.0 50-51 34.61535135135135 37.0 34.0 38.0 26.0 38.0 52-53 34.2150990990991 37.0 34.0 38.0 24.0 38.0 54-55 34.66965765765765 37.0 34.5 38.0 25.5 38.0 56-57 34.56151351351352 37.0 34.0 38.0 27.0 38.0 58-59 34.321729729729725 37.0 34.0 38.0 24.5 38.0 60-61 34.366882882882884 37.0 34.0 38.0 25.5 38.0 62-63 35.069117117117116 37.0 35.5 38.0 29.0 38.0 64-65 34.983531531531526 37.0 35.5 38.0 28.0 38.0 66-67 32.918126126126126 37.0 31.0 38.0 21.5 38.0 68-69 28.707963963963962 33.5 22.5 37.0 11.0 38.0 70-71 24.934774774774773 26.0 15.0 35.5 10.0 38.0 72-73 21.642666666666663 22.0 11.0 31.0 10.0 37.0 74-75 21.151711711711712 20.0 11.0 30.0 10.0 37.0 76 21.60381981981982 21.0 11.0 31.0 10.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.8354017785617742 1101 2 0.6703439826386486 1101 3 0.45986319719796853 1101 4 0.5518952092671867 1101 5 0.17164497234063703 1101 6 -1.967603683032479 1101 7 -0.038122613851747644 1101 8 0.6745041092005195 1101 9 0.8962081390660046 1101 10-11 -0.285935969714437 1101 12-13 1.0039403450380746 1101 14-15 1.5729297928595969 1101 16-17 0.768113710634438 1101 18-19 0.2755236503709497 1101 20-21 0.539393922736437 1101 22-23 -0.01431524178667587 1101 24-25 -2.6821841050642306 1101 26-27 -0.8465397628450333 1101 28-29 1.4118943738419034 1101 30-31 1.3763199683937302 1101 32-33 0.9715246711385745 1101 34-35 1.524890377835895 1101 36-37 1.4685074608411384 1101 38-39 0.1104327837489194 1101 40-41 1.8830645929912038 1101 42-43 1.6906414065896058 1101 44-45 1.199781485755139 1101 46-47 1.9609546767158719 1101 48-49 -1.987303754472947 1101 50-51 -3.5058490438264656 1101 52-53 -4.373528533471422 1101 54-55 -3.748031207673101 1101 56-57 -2.426471896751792 1101 58-59 -2.0527145931648576 1101 60-61 -2.328095595003859 1101 62-63 -0.7180774690826652 1101 64-65 -3.4811878416731687 1101 66-67 -2.449230568854645 1101 68-69 -1.228682533223143 1101 70-71 -0.35370861688586075 1101 72-73 1.320779592807778 1101 74-75 1.4740399503724149 1101 76 -0.9567949776225717 1102 1 -1.929341172501168 1102 2 -0.3478378355431673 1102 3 0.2810753184100889 1102 4 0.3094709668429445 1102 5 0.08073588143155064 1102 6 0.37179025636146434 1102 7 -0.6896377653669035 1102 8 -0.19216255746614763 1102 9 0.04772329058115332 1102 10-11 0.07770039392192274 1102 12-13 -0.5854535943558687 1102 14-15 -0.21343384350404193 1102 16-17 -1.2273408348201045 1102 18-19 -1.2911430162957132 1102 20-21 0.31666665000916794 1102 22-23 0.9796241521527165 1102 24-25 -5.092790165670287 1102 26-27 -3.1571458234511027 1102 28-29 -0.9320450200974904 1102 30-31 0.7066229986967585 1102 32-33 -1.467869268255356 1102 34-35 0.3354964384419574 1102 36-37 -2.217856175522499 1102 38-39 -0.7638096404935091 1102 40-41 0.731549441476055 1102 42-43 0.7285201944683948 1102 44-45 -0.012339726366072057 1102 46-47 0.8442880100492118 1102 48-49 -5.6448795120487105 1102 50-51 -2.702818740796161 1102 52-53 -1.2750436849865778 1102 54-55 -0.3843948440367342 1102 56-57 0.8235281032482078 1102 58-59 -0.043623684073949676 1102 60-61 -8.106883473791736 1102 62-63 -3.928683529688726 1102 64-65 -0.37361208409740954 1102 66-67 1.1234967038726253 1102 68-69 -2.248379502920116 1102 70-71 -1.7476480108252552 1102 72-73 0.05714322917141246 1102 74-75 0.4437369200693837 1102 76 2.30684138601379 1103 1 1.0523175196838395 1103 2 0.4615720528140841 1103 3 -0.2173297852581726 1103 4 0.4466320513724469 1103 5 0.13655725304239041 1103 6 0.26572965030085527 1103 7 0.7074914212359715 1103 8 0.16748656534087303 1103 9 0.8260327004695114 1103 10-11 0.1351166618645081 1103 12-13 -0.9136035146110473 1103 14-15 -0.81829827731584 1103 16-17 -0.16434242971643442 1103 18-19 0.9895587380902455 1103 20-21 -0.21762362112320943 1103 22-23 1.120772477511565 1103 24-25 -3.9620086664677387 1103 26-27 -0.9211011663538144 1103 28-29 -1.4854740472107295 1103 30-31 -0.034206347395752346 1103 32-33 1.6250334430684035 1103 34-35 -0.7426534818132211 1103 36-37 -0.20868552161500276 1103 38-39 -0.41851458467213476 1103 40-41 -0.5686897929737 1103 42-43 1.1774835118527633 1103 44-45 0.5857463980358446 1103 46-47 1.2302529223299103 1103 48-49 -1.8478300702624253 1103 50-51 -0.2953227280369859 1103 52-53 -0.5314232703135318 1103 54-55 0.15547756425672077 1103 56-57 1.0515982786868037 1103 58-59 -1.3430654703578426 1103 60-61 -5.359674542372282 1103 62-63 -1.565445890135294 1103 64-65 0.19775952674788755 1103 66-67 -1.6623884635914905 1103 68-69 -1.708507094626654 1103 70-71 -1.1168665116227032 1103 72-73 -2.1099221615781865 1103 74-75 -1.3242056636626742 1103 76 2.890573443430057 1104 1 -0.024290667450664216 1104 2 -0.0629893506946857 1104 3 0.3820854194201857 1104 4 -0.3147714573994804 1104 5 -0.6950216943260301 1104 6 0.09906298363419097 1104 7 0.6174329417038038 1104 8 1.0078374425338552 1104 9 1.4517636946215546 1104 10-11 -1.0526026363811027 1104 12-13 0.7650514561491804 1104 14-15 0.9340409039707112 1104 16-17 -0.08188628936556341 1104 18-19 0.9088569837042826 1104 20-21 -0.7883838550413387 1104 22-23 0.17457364710220702 1104 24-25 1.3344825616024352 1104 26-27 1.4590157927105167 1104 28-29 1.9396721516196749 1104 30-31 1.1763199683937273 1104 32-33 0.882635782249686 1104 34-35 0.14155704450256934 1104 36-37 1.5296185719522484 1104 38-39 2.4270994504155823 1104 40-41 2.249731259657878 1104 42-43 1.3961969621451615 1104 44-45 1.3331148190884718 1104 46-47 1.8553991211603176 1104 48-49 0.5349184677492644 1104 50-51 1.1274842895068673 1104 52-53 1.3542492443063523 1104 54-55 0.5186354589935576 1104 56-57 0.06797254769265493 1104 58-59 -0.9971590376093076 1104 60-61 0.5274599605517025 1104 62-63 0.6763669753617805 1104 64-65 1.4910343805490527 1104 66-67 1.7063249867009063 1104 68-69 1.3657619112212984 1104 70-71 -0.14259750577475216 1104 72-73 -0.7347759627477792 1104 74-75 -1.5926267162942516 1104 76 -0.7012394220670188 1105 1 0.9684741904304772 1105 2 0.5711191764371009 1105 3 -0.018431376445441572 1105 4 -0.33182572096536944 1105 5 0.1793969103251385 1105 6 0.2541017433241137 1105 7 0.9657533551404995 1105 8 -0.5565036427374608 1105 9 1.2140375964303445 1105 10-11 0.8884826349367287 1105 12-13 0.11363026751868688 1105 14-15 0.9035499478983553 1105 16-17 0.8026098346654464 1105 18-19 1.5887019449445958 1105 20-21 1.3490838452170593 1105 22-23 0.8570025876706922 1105 24-25 2.1151027166411964 1105 26-27 1.411212175139461 1105 28-29 2.1828246064000396 1105 30-31 2.4786455497890785 1105 32-33 2.417261105247107 1105 34-35 -0.5119313275904531 1105 36-37 1.4378873058023771 1105 38-39 0.579037434911708 1105 40-41 0.8753126550067094 1105 42-43 1.26622280193844 1105 44-45 1.300556679553587 1105 46-47 0.8520399480337062 1105 48-49 0.2522311292479742 1105 50-51 1.64531374687121 1105 52-53 0.5043784432727634 1105 54-55 1.8837517380633315 1105 56-57 1.58709399472108 1105 58-59 1.5434094378428966 1105 60-61 2.341671846856606 1105 62-63 1.1319225309173362 1105 64-65 2.7060214606524085 1105 66-67 2.4786764078895374 1105 68-69 2.0779066140636786 1105 70-71 1.90210533660251 1105 72-73 0.2037253292418839 1105 74-75 0.08644305114760797 1105 76 1.8501817665634732 1106 1 -0.7918120349720255 1106 2 0.23957475186941934 1106 3 0.33935037668514667 1106 4 -0.3455406881687111 1106 5 -0.13604733535166957 1106 6 0.30419118876239537 1106 7 0.05162097589183645 1106 8 0.03860667330308587 1106 9 -2.0268687840109223 1106 10-11 0.8935512097727454 1106 12-13 1.353940345038069 1106 14-15 -1.4713021732859488E-4 1106 16-17 0.6258060183267489 1106 18-19 0.45244672729402424 1106 20-21 1.1663169996595144 1106 22-23 0.9831206556492162 1106 24-25 -0.30141487429499847 1106 26-27 1.4034602371549596 1106 28-29 1.4234328353803676 1106 30-31 1.0609353530091141 1106 32-33 0.26639646601037015 1106 34-35 1.685146788092311 1106 36-37 1.9313279736616522 1106 38-39 0.10915073246686546 1106 40-41 1.7163979263245395 1106 42-43 1.2867952527434525 1106 44-45 1.597217383191044 1106 46-47 0.998134163895358 1106 48-49 2.3306449634757627 1106 50-51 1.827484289506863 1106 52-53 2.6969842870413956 1106 54-55 -0.11982607946797685 1106 56-57 1.413271692991799 1106 58-59 1.4703623299120636 1106 60-61 1.9001095332012738 1106 62-63 1.2985891975840076 1106 64-65 -1.0927263032116343 1106 66-67 1.0430771234530454 1106 68-69 4.067471312930699 1106 70-71 0.3257785626013181 1106 72-73 0.8951385671667502 1106 74-75 0.5637835401160025 1106 76 -1.6465385673661643 1107 1 0.6423759992160072 1107 2 -0.04076712847246 1107 3 0.20430764164240856 1107 4 0.3518952092671839 1107 5 -0.3172439165482501 1107 6 -0.21204812747691903 1107 7 0.46187738614825236 1107 8 -0.19216255746614763 1107 9 -1.0149029720451068 1107 10-11 0.6029529191744487 1107 12-13 -0.09050409940637394 1107 14-15 0.6118186817484883 1107 16-17 0.8847803773011051 1107 18-19 0.41996809481539543 1107 20-21 0.7005050338475485 1107 22-23 -1.0920930195644587 1107 24-25 -0.04329521617534482 1107 26-27 -0.6965397628450418 1107 28-29 0.7285610405085663 1107 30-31 0.6540977461715016 1107 32-33 1.882635782249693 1107 34-35 -0.025109622164102063 1107 36-37 1.8740630163966898 1107 38-39 -0.10623388291774916 1107 40-41 -0.8613798514532363 1107 42-43 1.429530295478493 1107 44-45 0.49978148575514325 1107 46-47 0.899843565604769 1107 48-49 1.5793629121937158 1107 50-51 0.9385954006179773 1107 52-53 0.5431381331952423 1107 54-55 0.5186354589935647 1107 56-57 1.2790836588037635 1107 58-59 0.04728540683514382 1107 60-61 1.6941266272183597 1107 62-63 0.5319225309173348 1107 64-65 0.4910343805490527 1107 66-67 -1.1158972355213166 1107 68-69 0.987984133443522 1107 70-71 0.20184693866969639 1107 72-73 -0.04588707385888924 1107 74-75 1.5851510614835256 1107 76 0.12098280015520402 1108 1 -0.40719665035664576 1108 2 0.39342090571557264 1108 3 0.05729909463386207 1108 4 0.4236900810620554 1108 5 -0.23861143791577177 1108 6 -0.5676036830324804 1108 7 -1.4099174856466234 1108 8 -1.1665215318251185 1108 9 0.5372337800916398 1108 10-11 0.17560249182402288 1108 12-13 1.0206070117047403 1108 14-15 0.1665195364493428 1108 16-17 -0.11778372526299563 1108 18-19 0.41398518883249125 1108 20-21 1.1406759740184924 1108 22-23 -0.17072549819692995 1108 24-25 2.1985851257050015 1108 26-27 1.3137166474113755 1108 28-29 0.4234328353803605 1108 30-31 1.4327302248039828 1108 32-33 2.163832363446268 1108 34-35 1.0441211470666687 1108 36-37 1.367225409559083 1108 38-39 1.0578686811848144 1108 40-41 1.472808182734795 1108 42-43 0.4791029450511388 1108 44-45 1.4690122549859126 1108 46-47 1.2801854459466426 1108 48-49 1.5870552198860253 1108 50-51 -0.05713109510851666 1108 52-53 -0.8414772514201374 1108 54-55 -0.73521069485259 1108 56-57 0.0542973340174413 1108 58-59 0.8421572017069394 1108 60-61 1.6436992767910183 1108 62-63 2.40115330014811 1108 64-65 1.548299337814015 1108 66-67 1.889230969606885 1108 68-69 2.080291825751214 1108 70-71 -0.7126829758602184 1108 72-73 -0.5663998943717097 1108 74-75 -1.9362164598839975 1108 76 -2.0824360032635987 1109 1 -0.6736131606755933 1109 2 0.5597748769475928 1109 3 0.32246482321422576 1109 4 0.18604155073060014 1109 5 0.26107586664958404 1109 6 -0.6001240082357313 1109 7 -0.8470657032826452 1109 8 0.43385370269645307 1109 9 0.8067772447570576 1109 10-11 0.5067469571148351 1109 12-13 0.32548506048522796 1109 14-15 1.284718410745775 1109 16-17 1.7855933854311843 1109 18-19 1.6885317804522444 1109 20-21 1.719475223549452 1109 22-23 0.630400205367792 1109 24-25 0.5328565453422769 1109 26-27 1.12703747292732 1109 28-29 -0.8185934310361489 1109 30-31 2.334856553759579 1109 32-33 1.9186791426832954 1109 34-35 0.9293619225513439 1109 36-37 2.190865184418371 1109 38-39 1.062871808139164 1109 40-41 2.147292235267628 1109 42-43 1.5138121382969274 1109 44-45 1.8623831117714005 1109 46-47 1.982499392163028 1109 48-49 2.380988928453874 1109 50-51 1.128297297636955 1109 52-53 0.7057397592115038 1109 54-55 -0.1423401507625357 1109 56-57 0.8519833878010559 1109 58-59 -0.7047471134900647 1109 60-61 2.028001965971754 1109 62-63 0.14411765286855882 1109 64-65 1.0273487436926843 1109 66-67 2.411745040901451 1109 68-69 -0.05510529745078685 1109 70-71 0.9267791879921887 1109 72-73 0.7581779667915178 1109 74-75 0.5268854788276975 1109 76 0.8212538028652325 1110 1 -1.2687351118951042 1110 2 0.5751058874005537 1110 3 -0.42108918375441107 1110 4 -0.24810479073281044 1110 5 0.5049783056739727 1110 6 -0.06284177827057391 1110 7 0.842829767100632 1110 8 0.7411707758671895 1110 9 1.0485890914469564 1110 10-11 -0.45736454114300784 1110 12-13 0.33489272599045705 1110 14-15 1.3610250309548348 1110 16-17 1.2228756153963403 1110 18-19 -0.24352396867667636 1110 20-21 0.8989177322602444 1110 22-23 0.4809228534514176 1110 24-25 -0.24170791458804075 1110 26-27 1.9367935704882981 1110 28-29 1.2476086595561853 1110 30-31 0.9763199683937245 1110 32-33 0.552477052090957 1110 34-35 2.7129856159311387 1110 36-37 2.994697937031617 1110 38-39 1.1604327837489166 1110 40-41 -1.0074115974849818 1110 42-43 0.0549271208753197 1110 44-45 1.0759719619456192 1110 46-47 0.8490499148111113 1110 48-49 2.4984105312413334 1110 50-51 0.4370080990306775 1110 52-53 1.0336143236714364 1110 54-55 0.9614926018507077 1110 56-57 2.442575722295828 1110 58-59 -1.0193812598315262 1110 60-61 1.0433329764247148 1110 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64-65 -3.3130060234913508 2116 66-67 -0.45226087188495256 2116 68-69 -2.1120158665564794 2116 70-71 0.08568532250807692 2116 72-73 -2.4428567708285875 2116 74-75 -0.8592933829609173 2116 76 0.3068413860137902 2117 1 0.8888406456806521 2117 2 0.712768225062888 2117 3 0.09925713659190905 2117 4 0.24886490623688218 2117 5 0.08073588143155064 2117 6 0.5536084381796442 2117 7 0.9164228406937056 2117 8 0.41389804859446144 2117 9 -2.3765191336612688 2117 10-11 -0.6950268788053435 2117 12-13 -0.47939298829525967 2117 14-15 -0.0013126313828308867 2117 16-17 -1.3637044711837447 2117 18-19 -2.3517490769017755 2117 20-21 0.1803030136455277 2117 22-23 0.2068968794254431 2117 24-25 -1.7897598626399898 2117 26-27 -2.2783579446632203 2117 28-29 -2.022954111006584 2117 30-31 -0.5206497285759681 2117 32-33 -2.907263207649301 2117 34-35 1.850647953593473 2117 36-37 -3.0208864785528036 2117 38-39 -0.9759308526147166 2117 40-41 0.30730701723363296 2117 42-43 0.5315504974986993 2117 44-45 0.47250875848241236 2117 46-47 -0.9890453232841239 2117 48-49 -1.523667390836593 2117 50-51 -0.672515710493137 2117 52-53 1.1037441938013046 2117 54-55 0.05499909535720349 2117 56-57 1.0962553759754812 2117 58-59 -0.9072600477103165 2117 60-61 -0.7583986253068886 2117 62-63 0.25313465212946085 2117 64-65 -1.5251272356125583 2117 66-67 0.45683003720596105 2117 68-69 -0.08171283625344472 2117 70-71 -0.8688601320373763 2117 72-73 -0.6095234374952518 2117 74-75 1.7467672230996847 2117 76 3.5795686587410636 2118 1 0.30269345953346516 2118 2 -1.0534655411708727 2118 3 -0.8782320408972737 2118 4 0.20903806641004508 2118 5 0.5049783056739727 2118 6 0.48001536458657057 2118 7 1.0428297671006277 2118 8 0.9983136330100422 2118 9 -0.7228394799816158 2118 10-11 0.32834974457127686 2118 12-13 -1.5651072740095486 2118 14-15 1.189596459526264 2118 16-17 -2.0056958131750875 2118 18-19 0.6136188884661848 2118 20-21 0.12748916083167927 2118 22-23 0.5380657105942745 2118 24-25 0.47257779969767455 2118 26-27 -1.7203492866545602 2118 28-29 -0.7666770547295272 2118 30-31 1.047748539822301 2118 32-33 1.552477052090957 2118 34-35 -0.7155858126402919 2118 36-37 -3.2053020629683857 2118 38-39 0.37471849803463186 2118 40-41 0.5354455453721627 2118 42-43 1.0977842637324642 2118 44-45 -1.5240280380543823 2118 46-47 0.2633356290968294 2118 48-49 0.11269624552704727 2118 50-51 -0.36299190096932676 2118 52-53 -1.9520999620428512 2118 54-55 -0.938507398149298 2118 56-57 0.45686143658154066 2118 58-59 -2.13366697411724 2118 60-61 -1.4138098807181407 2118 62-63 -0.9395060405112332 2118 64-65 1.1545264440411174 2118 66-67 0.06505514543106727 2118 68-69 -3.359634914175526 2118 70-71 -1.067994331171576 2118 72-73 1.0731605451887312 2118 74-75 2.2645161408486025 2118 76 0.6289193080917137 2119 1 0.9090426658826729 2119 2 -1.2333597210650566 2119 3 0.3820854194201857 2119 4 -1.1888455314735538 2119 5 -0.7172439165482487 2119 6 -0.5676036830324804 2119 7 -3.3899744657036024 2119 8 -0.22919959450318572 2119 9 -0.21490297204510966 2119 10-11 -2.193343377121842 2119 12-13 -1.3497633586656264 2119 14-15 -1.5215146515848517 2119 16-17 -2.0337381412174196 2119 18-19 -1.9948467199994226 2119 20-21 -0.5031986698561539 2119 22-23 -0.32172264919408633 2119 24-25 -0.7358878087679344 2119 26-27 0.8108676445623715 2119 28-29 -1.9269945150469852 2119 30-31 -0.508865216791456 2119 32-33 -2.0840308844169755 2119 34-35 -0.4658503629048383 2119 36-37 -1.8037147613810873 2119 38-39 -1.2654931421770073 2119 40-41 -2.302120592193976 2119 42-43 -1.8000993341511347 2119 44-45 -1.6705888846152277 2119 46-47 -0.989045323284131 2119 48-49 -2.1465630137322123 2119 50-51 -0.24658978456720604 2119 52-53 -2.16056557050846 2119 54-55 -1.748031207673101 2119 56-57 -3.046842267122159 2119 58-59 -2.0267886672389324 2119 60-61 -2.005873372781636 2119 62-63 -1.0347441357493352 2119 64-65 0.7021454916601613 2119 66-67 -0.3158972355213123 2119 68-69 0.36946561492500507 2119 70-71 1.4425876794104369 2119 72-73 2.1059647779929627 2119 74-75 -0.5444785681461077 2119 76 0.5357976149700185 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 7.0 23 21.0 24 28.0 25 67.0 26 139.0 27 211.0 28 366.0 29 567.0 30 829.0 31 1129.0 32 1546.0 33 2196.0 34 2813.0 35 2383.0 36 832.0 37 740.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 5.578378378378378 29.90990990990991 11.63963963963964 52.87207207207207 2 2.6522522522522523 5.96036036036036 80.74234234234234 10.645045045045046 3 2.7315315315315316 34.9045045045045 56.61981981981982 5.744144144144144 4 2.1117117117117115 39.524324324324326 27.581981981981983 30.781981981981982 5 1.6 51.87747747747748 40.68468468468468 5.837837837837838 6 3.5747747747747747 44.30990990990991 9.448648648648648 42.66666666666667 7 3.790990990990991 51.754954954954954 7.7981981981981985 36.655855855855854 8 3.2504504504504506 42.846846846846844 29.693693693693696 24.20900900900901 9 3.344144144144144 23.675675675675674 6.378378378378378 66.6018018018018 10-11 3.5495495495495493 25.22162162162162 40.57297297297297 30.655855855855858 12-13 16.699099099099097 6.007207207207207 31.798198198198197 45.4954954954955 14-15 24.53333333333333 18.216216216216218 9.643243243243244 47.60720720720721 16-17 30.245045045045043 26.547747747747746 4.075675675675676 39.131531531531536 18-19 24.17297297297297 23.08108108108108 3.8162162162162163 48.92972972972973 20-21 18.882882882882882 12.691891891891894 3.794594594594595 64.63063063063062 22-23 24.944144144144147 37.48468468468468 3.6756756756756754 33.895495495495496 24-25 30.252252252252255 42.9009009009009 4.241441441441442 22.605405405405403 26-27 37.207207207207205 32.263063063063065 4.018018018018018 26.511711711711712 28-29 27.135135135135137 10.443243243243243 4.403603603603604 58.01801801801801 30-31 44.5981981981982 17.466666666666665 4.288288288288288 33.64684684684684 32-33 45.96036036036036 25.574774774774774 4.500900900900901 23.963963963963963 34-35 11.437837837837836 45.693693693693696 16.572972972972973 26.295495495495498 36-37 3.499099099099099 47.66486486486487 25.019819819819823 23.816216216216215 38-39 16.317117117117117 13.827027027027029 29.935135135135134 39.92072072072072 40-41 11.816216216216215 17.88108108108108 39.30810810810811 30.994594594594595 42-43 35.023423423423424 12.021621621621621 43.41261261261261 9.542342342342343 44-45 13.7009009009009 22.605405405405403 32.41801801801802 31.275675675675675 46-47 36.09009009009009 6.331531531531532 23.333333333333332 34.245045045045046 48-49 27.42702702702703 4.335135135135135 26.0 42.23783783783784 50-51 8.407207207207207 4.288288288288288 45.7981981981982 41.50630630630631 52-53 15.834234234234234 4.554954954954955 61.560360360360356 18.05045045045045 54-55 11.03063063063063 4.49009009009009 47.64324324324324 36.836036036036035 56-57 34.37837837837838 4.673873873873874 32.49369369369369 28.454054054054055 58-59 13.037837837837838 32.01801801801802 44.76396396396397 10.18018018018018 60-61 21.394594594594597 61.81981981981982 12.0 4.7855855855855856 62-63 4.854054054054054 86.06126126126125 4.118918918918919 4.965765765765766 64-65 2.655855855855856 88.12972972972973 3.938738738738739 5.275675675675676 66-67 2.7099099099099098 86.16576576576577 3.981981981981982 7.142342342342342 68-69 3.300900900900901 77.51351351351352 5.621621621621622 13.563963963963966 70-71 4.273873873873875 68.78198198198197 8.018018018018019 18.926126126126125 72-73 5.488288288288288 57.524324324324326 10.699099099099099 26.288288288288285 74-75 6.27027027027027 54.87207207207207 11.066666666666666 27.790990990990995 76 5.167567567567567 57.30450450450451 9.852252252252253 27.675675675675677 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 1.0 19 3.5 20 4.0 21 3.0 22 6.5 23 8.0 24 3.5 25 1.0 26 6.5 27 12.0 28 18.0 29 24.0 30 21.5 31 17.5 32 27.0 33 38.0 34 38.0 35 49.0 36 66.5 37 73.0 38 77.5 39 101.0 40 159.5 41 369.0 42 539.0 43 1217.0 44 2453.0 45 2967.0 46 2923.0 47 2499.5 48 1633.5 49 918.5 50 646.0 51 481.5 52 238.5 53 138.0 54 116.0 55 100.0 56 69.0 57 49.5 58 45.0 59 33.5 60 29.5 61 26.5 62 16.0 63 12.0 64 8.0 65 6.0 66 2.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.5 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 13875.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.733333333333334 #Duplication Level Percentage of deduplicated Percentage of total 1 78.01396902520497 18.515315315315313 2 11.387792286668692 5.405405405405405 3 4.0996052232007285 2.918918918918919 4 1.579107197084725 1.499099099099099 5 1.032493167324628 1.2252252252252251 6 0.6377163680534468 0.908108108108108 7 0.39477679927118126 0.6558558558558558 8 0.4251442453689645 0.8072072072072072 9 0.12146978439113272 0.2594594594594595 >10 1.700576981475858 6.558558558558558 >50 0.3036744609778318 5.254054054054054 >100 0.21257212268448225 10.774774774774775 >500 0.03036744609778318 5.881081081081081 >1k 0.06073489219556636 39.33693693693694 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 2912 20.987387387387386 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT 2546 18.34954954954955 RNA PCR Primer, Index 15 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 816 5.881081081081081 No Hit CTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 428 3.0846846846846847 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 327 2.3567567567567567 No Hit TATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT 242 1.744144144144144 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 144 1.037837837837838 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGT 122 0.8792792792792792 No Hit TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 121 0.872072072072072 No Hit CTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 111 0.8 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTCTT 89 0.6414414414414414 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGT 83 0.5981981981981982 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 81 0.5837837837837837 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTCTT 76 0.5477477477477477 No Hit CTTATAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 73 0.5261261261261262 No Hit CTTAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 69 0.4972972972972973 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTCTT 67 0.4828828828828829 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGT 67 0.4828828828828829 No Hit TTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 65 0.46846846846846846 No Hit CTTATTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 59 0.4252252252252252 No Hit CTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 44 0.3171171171171171 No Hit CTTATCACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 36 0.2594594594594595 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGT 31 0.22342342342342342 No Hit CTTATACACATCTCCGAGCCCACGCGACCGCGGACAATCTCGTATGCCGT 28 0.2018018018018018 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCG 28 0.2018018018018018 No Hit CTTATACACATCTCCGAGCCCACGAGACAGCGGACAATCTCGTATGCCGT 25 0.18018018018018017 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGT 23 0.16576576576576577 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTAGTATGCCGT 22 0.15855855855855855 No Hit ACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCT 22 0.15855855855855855 RNA PCR Primer, Index 15 (95% over 23bp) CTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT 21 0.15135135135135136 No Hit TCTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 21 0.15135135135135136 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTCTGCCGTCTT 21 0.15135135135135136 No Hit CTTTACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTC 20 0.14414414414414414 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCAGTCTT 19 0.13693693693693693 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGTCTT 19 0.13693693693693693 Illumina PCR Primer Index 7 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCAGT 19 0.13693693693693693 No Hit CCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 18 0.12972972972972974 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGTCTT 18 0.12972972972972974 Illumina PCR Primer Index 4 (95% over 22bp) ATACACATCTCCGAGCCCACGAGACCGCGGACACTCTCGTATGCCGTCTT 18 0.12972972972972974 TruSeq Adapter, Index 19 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTC 18 0.12972972972972974 No Hit TCTTAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 17 0.12252252252252253 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAAATCGTATGCCGT 17 0.12252252252252253 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAGCTCGTATGCCGT 17 0.12252252252252253 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCG 17 0.12252252252252253 No Hit TCTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 16 0.11531531531531532 No Hit CTTATATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 14 0.1009009009009009 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTATT 14 0.1009009009009009 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGACAATCTCGTATGCCGTCTT 14 0.1009009009009009 RNA PCR Primer, Index 19 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGACGTCTT 14 0.1009009009009009 No Hit ATACACATCTCCGAGCCCACGAGACAGCGGACAATCTCGTATGCCGTCTT 14 0.1009009009009009 RNA PCR Primer, Index 36 (95% over 21bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.021621621621621623 0.0 2 0.0 0.0 0.0 0.021621621621621623 0.0 3 0.0 0.0 0.0 0.021621621621621623 0.0 4 0.0 0.0 0.0 0.021621621621621623 0.0 5 0.0 0.0 0.0 0.021621621621621623 0.0 6 0.0 0.0 0.0 0.021621621621621623 0.0 7 0.0 0.0 0.0 0.021621621621621623 0.0 8 0.0 0.0 0.0 0.021621621621621623 0.0 9 0.0 0.0 0.0 0.021621621621621623 0.0 10 0.0 0.0 0.0 0.021621621621621623 0.0 11 0.0 0.0 0.0 0.02882882882882883 0.0 12 0.0 0.0 0.0 0.02882882882882883 0.0 13 0.0 0.0 0.0 0.02882882882882883 0.0 14 0.0 0.0 0.0 0.02882882882882883 0.0 15 0.0 0.0 0.0 0.02882882882882883 0.0 16 0.0 0.0 0.0 0.02882882882882883 0.0 17 0.0 0.0 0.0 0.02882882882882883 0.0 18 0.0 0.0 0.0 0.036036036036036036 0.0 19 0.0 0.0 0.0 0.06486486486486487 0.0 20 0.0 0.0 0.0 0.06486486486486487 0.0 21 0.0 0.0 0.0 0.08648648648648649 0.0 22 0.0 0.0 0.0 0.08648648648648649 0.0 23 0.0 0.0 0.0 0.08648648648648649 0.0 24 0.0 0.0 0.0 0.08648648648648649 0.0 25 0.0 0.0 0.0 0.0936936936936937 0.0 26 0.0 0.0 0.0 0.0936936936936937 0.0 27 0.0 0.0 0.0 0.0936936936936937 0.0 28 0.0 0.0 0.0 0.1009009009009009 0.0 29 0.0 0.0 0.0 0.1009009009009009 0.0 30 0.0 0.0 0.0 0.11531531531531532 0.0 31 0.0 0.0 0.0 0.13693693693693693 0.0 32 0.0 0.0 0.0 0.13693693693693693 0.0 33 0.0 0.0 0.0 0.15855855855855855 0.0 34 0.0 0.0 0.0 0.1945945945945946 0.0 35 0.0 0.0 0.0 0.22342342342342342 0.0 36 0.0 0.0 0.0 0.24504504504504504 0.0 37 0.0 0.0 0.0 0.3171171171171171 0.0 38 0.0 0.0 0.0 0.4828828828828829 0.0 39 0.0 0.0 0.0 0.6702702702702703 0.0 40 0.0 0.0 0.0 0.8072072072072072 0.0 41 0.0 0.0 0.0 0.9585585585585585 0.0 42 0.0 0.0 0.0 1.045045045045045 0.0 43 0.0 0.0 0.0 1.2396396396396396 0.0 44 0.0 0.0 0.0 1.3837837837837839 0.0 45 0.0 0.0 0.0 1.5351351351351352 0.0 46 0.0 0.0 0.0 1.7009009009009008 0.0 47 0.0 0.0 0.0 1.8306306306306306 0.0 48 0.0 0.0 0.0 1.9315315315315316 0.0 49 0.0 0.0 0.0 2.075675675675676 0.0 50 0.0 0.0 0.0 2.1837837837837837 0.0 51 0.0 0.0 0.0 2.3135135135135134 0.0 52 0.0 0.0 0.0 2.4144144144144146 0.0 53 0.0 0.0 0.0 2.4504504504504503 0.0 54 0.0 0.0 0.0 2.572972972972973 0.0 55 0.0 0.0 0.0 2.630630630630631 0.0 56 0.0 0.0 0.0 2.717117117117117 0.0 57 0.0 0.0 0.0 2.7963963963963963 0.0 58 0.0 0.0 0.0 2.962162162162162 0.0 59 0.0 0.0 0.0 3.027027027027027 0.0 60 0.0 0.0 0.0 3.120720720720721 0.0 61 0.0 0.0 0.0 3.1855855855855855 0.0 62 0.0 0.0 0.0 3.2432432432432434 0.0 63 0.0 0.0 0.0 3.344144144144144 0.0 64 0.0 0.0 0.0 3.409009009009009 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTCC 15 0.0021786576 70.0 59 CCTATTT 15 0.0021786576 70.0 38 TTTCCTC 15 0.0021786576 70.0 61 CCAAATC 15 0.0021786576 70.0 23 CAACCTT 15 0.0021786576 70.0 55 TAAAAAA 15 0.0021786576 70.0 61 CATCCCT 15 0.0021786576 70.0 2 TTTTTTA 15 0.0021786576 70.0 15 TATAACA 15 0.0021786576 70.0 3 TTATTAC 15 0.0021786576 70.0 2 TAGCTGT 15 0.0021786576 70.0 44 TTTTCCT 15 0.0021786576 70.0 60 TTTTTAA 15 0.0021786576 70.0 16 AACCTTT 15 0.0021786576 70.0 56 ACCAAAT 15 0.0021786576 70.0 22 GGACAAA 20 7.655769E-5 70.0 29 TCTACTA 15 0.0021786576 70.0 8 CAACAAA 15 0.0021786576 70.0 70 AACCTAT 15 0.0021786576 70.0 36 TTATAAC 15 0.0021786576 70.0 2 >>END_MODULE