FastQCFastQC Report
Wed 25 May 2016
SRR1780515_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780515_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95838
Sequences flagged as poor quality0
Sequence length50
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG1450.15129698032095829No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACAA207.7687483E-444.059121
CTTTACA950.041.6965831
TTTACAC1000.037.372052
CTTATAG250.002338610435.210451
ACGATAA250.00234461735.19206224
GACGATA250.00234461735.19206223
TTACACA1200.034.8073043
CGACGAT401.8054998E-532.9925622
CTATACA950.032.4306761
CTTTATA353.2100067E-431.4050872
GCTGCCT300.005681534329.37273417
AGATCCC300.00572527829.32671740
AAGCACG300.00573991829.31141530
CCAAAAT300.00573991829.31141532
CTTATAC6950.028.1810251
TTATACA7100.027.8664842
TCTTTAT406.9724966E-427.5081651
GACGAAT406.9938437E-427.49380123
CTGACGG450.001378010324.47727812
CGAATGA450.001390670524.43893225
Produced by FastQC (version 0.11.5)