##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780515_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 95838 Sequences flagged as poor quality 0 Sequence length 50 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.383480456603852 22.0 10.0 30.0 7.0 33.0 2 20.639015839228698 22.0 10.0 30.0 7.0 34.0 3 20.844529309877085 23.0 10.0 30.0 7.0 34.0 4 22.909378325924997 25.0 10.0 35.0 7.0 37.0 5 23.03823118178593 25.0 10.0 35.0 7.0 37.0 6 23.268640831402994 26.0 11.0 35.0 8.0 37.0 7 23.010079509171728 25.0 11.0 35.0 8.0 37.0 8 22.97545858636449 25.0 11.0 35.0 8.0 37.0 9 23.234468582399465 25.0 10.0 35.0 8.0 39.0 10 23.172405517644357 25.0 10.0 35.0 8.0 39.0 11 23.38411694734865 25.0 10.0 35.0 8.0 39.0 12 23.23925791439721 25.0 10.0 35.0 8.0 39.0 13 23.166113650117907 25.0 10.0 35.0 8.0 39.0 14 23.60850602057639 25.0 10.0 36.0 8.0 40.0 15 23.48172958534193 25.0 10.0 36.0 8.0 40.0 16 23.324766793964816 24.0 10.0 36.0 8.0 40.0 17 23.424236732819967 24.0 10.0 36.0 8.0 40.0 18 23.104227968029384 23.0 10.0 36.0 7.0 40.0 19 23.20509609966819 23.0 10.0 36.0 7.0 40.0 20 23.246217575491976 23.0 10.0 36.0 7.0 40.0 21 23.1187629124147 23.0 10.0 36.0 7.0 40.0 22 23.118355975709008 23.0 10.0 36.0 7.0 40.0 23 23.010684697093012 23.0 10.0 35.0 7.0 40.0 24 22.643356497422733 22.0 9.0 35.0 7.0 40.0 25 22.559611010246456 22.0 9.0 35.0 7.0 40.0 26 22.236858031261086 21.0 9.0 34.0 7.0 40.0 27 22.062146538951147 20.0 9.0 34.0 7.0 40.0 28 21.977983680794676 20.0 9.0 34.0 7.0 40.0 29 21.768453014461905 20.0 8.0 34.0 7.0 40.0 30 21.616185646611992 20.0 8.0 34.0 7.0 40.0 31 21.492372545336924 19.0 8.0 33.0 7.0 40.0 32 21.23281996702769 18.0 8.0 33.0 7.0 40.0 33 21.229376656441076 18.0 8.0 33.0 7.0 40.0 34 20.959713266136607 18.0 8.0 33.0 7.0 40.0 35 20.80587032283645 17.0 8.0 33.0 7.0 40.0 36 20.6972286566915 17.0 8.0 32.0 7.0 40.0 37 20.53559131033619 16.0 8.0 32.0 7.0 40.0 38 20.600795091717274 17.0 8.0 32.0 7.0 40.0 39 20.350821177403535 16.0 8.0 32.0 7.0 40.0 40 19.791502326843215 15.0 8.0 31.0 7.0 40.0 41 19.945449612888417 15.0 8.0 31.0 7.0 40.0 42 20.01157161042593 15.0 8.0 31.0 7.0 40.0 43 20.007053569565308 15.0 8.0 31.0 7.0 40.0 44 19.937446524343162 15.0 8.0 31.0 7.0 40.0 45 19.970710991464763 15.0 8.0 31.0 7.0 40.0 46 19.84381977921075 15.0 8.0 31.0 7.0 40.0 47 19.748513115883053 15.0 8.0 31.0 7.0 40.0 48 19.667553579999584 15.0 8.0 31.0 7.0 40.0 49 19.407166259729962 15.0 8.0 30.0 7.0 40.0 50 19.424268035643482 15.0 8.0 30.0 7.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.2565169428448755 1101 2 1.032469779127723 1101 3 0.6877103641149205 1101 4 1.1304251973302328 1101 5 1.1896791018936952 1101 6 1.442225890466048 1101 7 0.4288923633882824 1101 8 1.2511069550049996 1101 9 1.061212878962916 1101 10 0.9036479153131758 1101 11 0.8075916675861698 1101 12 0.5705584451181451 1101 13 0.6957258645668567 1101 14 1.3365240176191655 1101 15 0.7966209253883285 1101 16 2.038028235580388 1101 17 1.4134174933047774 1101 18 0.9335854804212502 1101 19 1.2242309001305571 1101 20 1.2139825546624046 1101 21 1.2408714842474495 1101 22 0.9127725121365131 1101 23 0.6970348323434976 1101 24 0.5113393767959273 1101 25 1.140232364380111 1101 26 0.5928663929138835 1101 27 0.7711189799921101 1101 28 1.5532305695189663 1101 29 1.897458587897816 1101 30 1.1734880701817936 1101 31 0.8475295952428361 1101 32 1.1646919925184172 1101 33 0.2168830921321785 1101 34 0.4764166733355708 1101 35 0.29497669163515283 1101 36 0.16472826195679247 1101 37 -0.11668529111522119 1101 38 -0.06936294785509745 1101 39 0.05212469326283653 1101 40 1.1291703254060081 1101 41 1.067952733189209 1101 42 0.6720259322996256 1101 43 1.1801976446735054 1101 44 1.597097508788245 1101 45 0.7363671379181262 1101 46 1.1212490912306095 1101 47 1.2327260349174587 1101 48 1.1499686080496936 1101 49 0.6500999794009523 1101 50 1.0917969468487634 1102 1 -0.13037354476503538 1102 2 -0.43550584037667406 1102 3 0.22812802998222637 1102 4 0.41075093673870455 1102 5 0.6300268237162356 1102 6 0.8293661782358335 1102 7 0.9144539141476749 1102 8 0.657731655244806 1102 9 1.1770701931115966 1102 10 1.6275188185905556 1102 11 0.6433730424862496 1102 12 0.007608804830375959 1102 13 0.4349344976603824 1102 14 0.3156407242538606 1102 15 0.1385473842212619 1102 16 0.9324127279864634 1102 17 0.6877480288763209 1102 18 -0.25571308072983 1102 19 0.3260494452944229 1102 20 0.2835269191708001 1102 21 0.4808295178205917 1102 22 0.5014515688911096 1102 23 0.009035232023752116 1102 24 0.6535256277951262 1102 25 0.5633438751714763 1102 26 0.47720891889310124 1102 27 -0.009906199864005316 1102 28 0.007067499974599656 1102 29 0.7328402825420994 1102 30 0.2483281980794736 1102 31 -0.26008431363006324 1102 32 0.22504371114351684 1102 33 -0.3746936464769348 1102 34 0.8126476885234197 1102 35 -0.14831867207388072 1102 36 -0.9958432808089945 1102 37 0.8742219830654356 1102 38 -0.2748985193978619 1102 39 -0.5555891356740155 1102 40 -0.0708196825875973 1102 41 0.572998696418626 1102 42 1.531488362355578 1102 43 0.5277695871195469 1102 44 -0.5720171994451668 1102 45 0.5720485927542605 1102 46 -0.03232804705876191 1102 47 0.6963551316400789 1102 48 -0.3869518555794045 1102 49 -0.79769178718578 1102 50 0.08370342166890765 1103 1 -0.5474046257825727 1103 2 0.03042729546759304 1103 3 -0.4613909430746226 1103 4 -0.01949310332336296 1103 5 -0.8912032510474823 1103 6 0.21959517151179853 1103 7 -0.5886729960888424 1103 8 0.6894239484690523 1103 9 -0.15569888574296442 1103 10 -1.2225775748829015 1103 11 0.11927467412211712 1103 12 0.016636876490693453 1103 13 0.5208892632596687 1103 14 -0.5874955902239698 1103 15 0.3472745201595693 1103 16 0.6826360787176426 1103 17 0.13604821225902697 1103 18 -0.0039145195787497755 1103 19 -0.5069782501962408 1103 20 0.4754204631591392 1103 21 -0.8665631562754257 1103 22 -0.11786474276544823 1103 23 -0.17265471014016143 1103 24 -0.1839220611125718 1103 25 0.2313271356219424 1103 26 -0.37486236525604966 1103 27 0.27316146365224014 1103 28 0.8685900466431491 1103 29 0.24508930685206565 1103 30 -0.5332112762234367 1103 31 0.7045557390336867 1103 32 0.5535808814073064 1103 33 0.026115118275967575 1103 34 -0.13224345738338172 1103 35 0.07479695307306145 1103 36 -0.1792259864092216 1103 37 -0.7384173172590103 1103 38 0.5360292090076477 1103 39 -0.005064849220826062 1103 40 0.15817359337986403 1103 41 1.3061063279604497 1103 42 0.3750488081166168 1103 43 -0.5032173880062345 1103 44 0.8148262669581818 1103 45 0.3854684451076693 1103 46 -0.057672477396842226 1103 47 -0.335901416062935 1103 48 -0.6282176664601096 1103 49 -0.30128890948793696 1103 50 -0.6361442296218236 1104 1 -1.3307846444567097 1104 2 -1.1156311279017572 1104 3 -1.0488259170642174 1104 4 -0.41365076638858866 1104 5 -0.4506270205552845 1104 6 -0.26993397347953163 1104 7 -0.5295826933010588 1104 8 -0.15918216064126156 1104 9 -1.7283279373636127 1104 10 -0.4621400665462332 1104 11 -1.2407359968129228 1104 12 -1.3359041399158684 1104 13 -1.319580257882123 1104 14 -0.5985666853286773 1104 15 -0.3864856505753913 1104 16 -0.11021439480510153 1104 17 0.1924707812866373 1104 18 0.0505072491287315 1104 19 -0.2947600295746611 1104 20 -0.6091466970382733 1104 21 -0.03676457017431645 1104 22 -0.24978417720589263 1104 23 -1.1534470270669317 1104 24 -1.0564043873764106 1104 25 -0.36104314582397024 1104 26 -0.5455973259069786 1104 27 -1.1952925846337408 1104 28 0.5447271681584205 1104 29 -0.1588055844377827 1104 30 -0.4866593221084585 1104 31 -0.564885370743557 1104 32 -0.5008408873001784 1104 33 -0.8991883364392521 1104 34 -0.42862867813835237 1104 35 -0.6657659387503365 1104 36 -0.4965529171815284 1104 37 -0.17139050653472054 1104 38 -1.2697597732519235 1104 39 -1.2466281412042868 1104 40 -1.575053030603062 1104 41 -1.806338650030746 1104 42 -0.8210296232559315 1104 43 -2.2904656206326166 1104 44 -2.0892687043636826 1104 45 -1.759522884757608 1104 46 -2.006585375889575 1104 47 -1.4656866634952408 1104 48 -1.6324860404763832 1104 49 -1.614214079555964 1104 50 -1.1879649579131417 1105 1 0.3021387235659958 1105 2 0.9455032596280901 1105 3 0.40979461724221977 1105 4 0.8194888470726873 1105 5 0.9758270047634525 1105 6 0.8731033739097569 1105 7 0.9183607224758461 1105 8 0.45819856648402535 1105 9 0.3715053734441547 1105 10 1.2250699167848467 1105 11 1.0046575248341476 1105 12 0.032478974919470716 1105 13 1.0053285135734775 1105 14 1.272046460591941 1105 15 1.1202044426804534 1105 16 1.1383777572875253 1105 17 1.030737213687413 1105 18 0.7179397850570126 1105 19 0.9791058081511572 1105 20 0.2250200822562256 1105 21 0.42036927674193336 1105 22 1.2123953966103898 1105 23 0.5399800126231149 1105 24 0.6300847778260952 1105 25 0.1605138213337547 1105 26 0.1657600647313977 1105 27 0.6001752713828381 1105 28 0.9908685386138885 1105 29 0.9705362185085598 1105 30 0.40683979939444725 1105 31 0.47363334800222034 1105 32 -0.5899069773123422 1105 33 0.22033231950524623 1105 34 0.8378037226365294 1105 35 0.31792555109063514 1105 36 0.37563701839535213 1105 37 0.7347679833513006 1105 38 -0.7673945887675337 1105 39 0.6803439721443638 1105 40 0.23591241517789996 1105 41 0.022376942166395963 1105 42 -0.37732101398440676 1105 43 0.2898830751370802 1105 44 0.13292348376985075 1105 45 0.8461445477783194 1105 46 0.7734749926286959 1105 47 0.3703272122537342 1105 48 0.09887405322997367 1105 49 0.18215479912133503 1105 50 0.29295588724611576 1106 1 0.035045471373404524 1106 2 -0.8078079986500555 1106 3 -0.7629277740232183 1106 4 -0.9504681960631629 1106 5 -1.2844199972054042 1106 6 -0.7959497852096291 1106 7 -0.4575565855606669 1106 8 -1.3733299819319384 1106 9 -0.2636745084244687 1106 10 -1.040233466068205 1106 11 -0.9636170411225393 1106 12 -1.3686307440710443 1106 13 -1.040760621919631 1106 14 -1.5234609673658177 1106 15 -1.0697454409780391 1106 16 -0.2934282208760699 1106 17 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39 -0.6738130278978147 1107 40 0.3716950953989304 1107 41 -0.39652886624461914 1107 42 -0.5598831264406456 1107 43 -0.9205189158360483 1107 44 -0.7588384430871962 1107 45 -0.40513144665370504 1107 46 -0.7519816235606172 1107 47 -0.4332329176656984 1107 48 -1.1287380444626898 1107 49 -0.7917347693605485 1107 50 -0.46791642894741514 1108 1 -0.8745941682662348 1108 2 -0.3644746653167239 1108 3 -1.0492340803295228 1108 4 -0.37596369155865617 1108 5 -0.5853208981063069 1108 6 0.21305922379938025 1108 7 -0.14971874772282945 1108 8 -0.826393044995001 1108 9 0.3070462122962496 1108 10 -0.7666298624646011 1108 11 -0.5671305546360514 1108 12 0.14722511178481312 1108 13 -0.4942741354331446 1108 14 -1.0829204268252788 1108 15 -1.0489346301672278 1108 16 -1.292527319975168 1108 17 -0.2729713955841113 1108 18 -0.7839145195787509 1108 19 0.620342011241668 1108 20 0.2006492213290727 1108 21 -0.14996184908588361 1108 22 -0.485838598974599 1108 23 -0.1899096121009478 1108 24 0.38522826568481605 1108 25 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0.9011371184646961 2213 23 1.7916356417621877 2213 24 1.769157625508214 2213 25 1.3062091855721647 2213 26 1.7551640382413325 2213 27 1.818718317740455 2213 28 1.7259678763622333 2213 29 0.7652382052635254 2213 30 1.175051719924248 2213 31 1.67230884469096 2213 32 1.3240003074558686 2213 33 1.2534449022867022 2213 34 1.632505709391495 2213 35 1.827859325925072 2213 36 1.2299416541569386 2213 37 0.7612580495764654 2213 38 0.13989017944438942 2213 39 1.2233903297602602 2213 40 0.3220051304096856 2213 41 1.3468802534246755 2213 42 0.8544670654857889 2213 43 1.3561082406999958 2213 44 1.7488175235049503 2213 45 0.8792571305597754 2213 46 1.2039385687876347 2213 47 0.330592112915987 2213 48 1.6949005432961997 2213 49 2.215267381902791 2213 50 1.0942803905573726 2214 1 -0.09642423362571151 2214 2 0.3572246810885069 2214 3 0.12684435104302594 2214 4 -1.4247218614932997 2214 5 -1.1003535778448672 2214 6 -0.3228884886189185 2214 7 -1.1834442379189056 2214 8 -0.14717735872049076 2214 9 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2215 31 0.7072234727938564 2215 32 -0.09948034308249021 2215 33 -0.6746985405208825 2215 34 0.11558900893647817 2215 35 -0.733793149634689 2215 36 -0.033967883167925095 2215 37 -0.4366966289837002 2215 38 -1.3922087528437608 2215 39 -0.804451270455985 2215 40 -0.3573659557731297 2215 41 -0.19409375207156288 2215 42 0.04291595497535994 2215 43 0.24694934535377655 2215 44 -1.2933503370167436 2215 45 -0.7799310480229167 2215 46 -0.4351568044610019 2215 47 0.030911975960542293 2215 48 0.06819423163246086 2215 49 0.21571789323315116 2215 50 -0.5008901279811688 2216 1 0.7826607336945486 2216 2 1.0818978837028865 2216 3 0.30086395888616124 2216 4 -0.2302774170488533 2216 5 0.19948302346232083 2216 6 -0.009979991886893913 2216 7 0.5126341934536427 2216 8 1.2939991118677447 2216 9 1.3344971926884064 2216 10 1.9285662159667716 2216 11 1.7434576806580644 2216 12 1.4014407980593226 2216 13 1.2545493939786212 2216 14 1.1053148672923712 2216 15 2.2704771345386554 2216 16 0.56090405257385 2216 17 1.6532050750041236 2216 18 1.5107913627741922 2216 19 0.3745576975161775 2216 20 0.5203551036820144 2216 21 1.2715087391494109 2216 22 0.76367120494697 2216 23 1.288129603585329 2216 24 1.9982674813710908 2216 25 0.5115558937918756 2216 26 1.2346147589269556 2216 27 0.6350895682274036 2216 28 1.5262697852052405 2216 29 1.8635533591396474 2216 30 0.9635207499203524 2216 31 0.7347844972036199 2216 32 1.320737744152403 2216 33 0.39212819017139466 2216 34 0.6733120981721719 2216 35 1.2479995674521476 2216 36 0.8771465626103883 2216 37 1.6831513101919704 2216 38 1.3636435880926143 2216 39 2.0921573730013954 2216 40 1.7962454888047006 2216 41 1.5879690730584883 2216 42 2.348088023802891 2216 43 1.92652117408527 2216 44 1.9896628682653699 2216 45 1.7000109287677994 2216 46 2.006870006263288 2216 47 1.6232489107344534 2216 48 1.7851810263503474 2216 49 1.470769914041476 2216 50 1.4741498880252344 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 1.0 5 1.0 6 22.0 7 446.0 8 2754.0 9 6268.0 10 5725.0 11 4637.0 12 4099.0 13 4226.0 14 4363.0 15 3986.0 16 4013.0 17 3663.0 18 3248.0 19 3019.0 20 2793.0 21 2540.0 22 2427.0 23 2314.0 24 2114.0 25 2082.0 26 1937.0 27 1815.0 28 1856.0 29 1677.0 30 1580.0 31 1538.0 32 1467.0 33 1441.0 34 1439.0 35 1419.0 36 1662.0 37 2049.0 38 2852.0 39 8362.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.286672377410525 18.645926916407245 20.242149468274032 27.825251237908194 2 19.24866951893979 26.296566837107378 34.25232181988939 20.202441824063445 3 18.680676565418366 31.23115289501967 28.160313866252075 21.92785667330989 4 16.62701642354807 24.445418310064902 32.23251737306705 26.69504789331998 5 17.00369373317473 38.63081449946785 28.65669150023999 15.708800267117425 6 25.109038168576138 39.60641916567541 14.641373985266803 20.643168680481647 7 30.0882739623114 31.882969177153115 15.8194035768693 22.209353283666186 8 23.883509328436077 37.35756918068367 17.919153553988064 20.83976793689219 9 24.584194160979987 26.29958888958451 15.057701538012061 34.05851541142344 10 22.998424408109603 29.50008869225873 26.449074991913353 21.052411907718312 11 32.202930005425934 25.080345590383573 20.76150924496014 21.955215159230352 12 23.833175078518735 26.32907958304204 23.88117324206726 25.956572096371964 13 28.180860055723095 21.291648840145676 22.962298212478217 27.565192891653012 14 32.42129209320769 22.468146385749616 19.113212075676465 25.997349445366225 15 27.069332554159537 26.664440455816674 18.743999666068373 27.522227323955423 16 30.756207674943564 22.624571524120057 20.343198729203245 26.276022071733134 17 36.57442190500041 22.161699641038485 17.89068369646882 23.37319475749228 18 31.657172720146328 20.55186830415469 19.01332636529919 28.777632610399788 19 29.976475508390404 21.2274556955408 22.02519734434628 26.770871451722517 20 34.48495878833522 21.130915024475964 20.778419312999453 23.605706874189362 21 27.809685214825976 23.20559897059285 18.65486614568622 30.32984966889496 22 31.93761548338605 19.565103921998475 17.908406668545716 30.588873926069756 23 37.39459843045584 21.750292202371014 18.33569878109868 22.51941058607447 24 28.91371131219828 29.146726296211156 16.99859982027544 24.940962571315122 25 34.19385145362493 22.651495380761 17.35581188997338 25.798841275640694 26 32.83406049725487 27.270734609523412 17.369475815710917 22.525729077510803 27 29.76660194985491 27.563098891463646 19.154088641155717 23.516210517525728 28 34.54232334829349 24.273040392443377 18.70785930487423 22.476776954388892 29 30.839570350421198 24.302967671896365 20.976210607626385 23.881251370056056 30 34.19024746789183 22.94246632557168 19.86425811840869 23.003028088127806 31 32.72231907053457 22.064365272398927 21.83053749073562 23.382778166330887 32 29.798116870915887 23.86688657383244 21.59022109021065 24.744775465041023 33 33.82013675035231 21.986533743932355 22.008455556135498 22.18487394957983 34 30.30179658221373 22.721910786800706 23.027778647709123 23.948513983276438 35 32.235839405390735 23.32609558009896 21.53579556130864 22.90226945320166 36 33.83791458309409 21.213291714079904 23.347148419964714 21.60164528286129 37 31.353924198615914 21.48158199638842 25.127605607340065 22.036888197655603 38 33.22163414863299 19.763448268662636 24.455857943691083 22.55905963901329 39 30.016389848733176 21.10532304705035 26.7112776774436 22.16700942677287 40 25.651319366035214 32.05055827374231 22.95508719106762 19.343035169154852 41 30.379680763328498 17.912956332014492 26.810451921370483 24.89691098328653 42 25.00574172669381 21.460486480843514 30.810105438981104 22.723666353481576 43 25.369784653284484 18.654683243040115 29.821814423950144 26.153717679725258 44 25.61760930145179 18.25848266936637 31.877720142360637 24.246187886821204 45 25.10280764012107 20.089760985283373 32.01544723932784 22.791984135267718 46 24.800667919014817 20.89542892924233 28.449175537466083 25.85472761427677 47 29.172276318398616 22.10776793262156 27.536867153010427 21.183088595969398 48 28.896588430511056 22.45854249068576 24.95799459408689 23.686874484716295 49 27.343122214917088 23.784477630631475 26.13464408336203 22.737756071089404 50 28.72976611665988 23.622112986213303 23.709780101652107 23.93834079547471 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 1.5 2 3.0 3 2.0 4 1.0 5 3.5 6 6.0 7 6.5 8 7.0 9 7.0 10 7.0 11 11.5 12 16.0 13 16.5 14 17.0 15 24.5 16 32.0 17 71.5 18 111.0 19 111.5 20 112.0 21 159.5 22 207.0 23 273.5 24 340.0 25 405.0 26 470.0 27 630.5 28 791.0 29 944.5 30 1098.0 31 1290.5 32 1483.0 33 1760.0 34 2037.0 35 2224.0 36 2411.0 37 2730.0 38 3049.0 39 3413.5 40 3778.0 41 4062.0 42 4346.0 43 4666.0 44 4986.0 45 5191.0 46 5396.0 47 5688.5 48 5981.0 49 6141.0 50 6301.0 51 6455.0 52 6609.0 53 6559.0 54 6509.0 55 6321.5 56 6134.0 57 5887.5 58 5641.0 59 5277.5 60 4914.0 61 4485.0 62 4056.0 63 3669.5 64 3283.0 65 3157.5 66 3032.0 67 2772.5 68 2513.0 69 2335.0 70 2157.0 71 1969.0 72 1781.0 73 1645.0 74 1509.0 75 1443.5 76 1378.0 77 1201.5 78 1025.0 79 904.0 80 783.0 81 664.5 82 546.0 83 484.0 84 422.0 85 341.0 86 260.0 87 202.5 88 145.0 89 112.5 90 80.0 91 57.0 92 34.0 93 25.5 94 17.0 95 12.5 96 8.0 97 7.5 98 7.0 99 8.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11686387445480917 2 0.00834741960391494 3 0.0010434274504893676 4 0.0 5 0.0 6 0.0 7 0.0 8 0.002086854900978735 9 0.0 10 0.0010434274504893676 11 0.002086854900978735 12 0.0010434274504893676 13 0.007303992153425573 14 0.009390847054404306 15 0.010434274504893674 16 0.1565141175734051 17 0.006260564702936205 18 0.1700786744297669 19 0.2013814979444479 20 0.24416202341451199 21 0.2598134351718525 22 0.04695423527202154 23 0.014607984306851146 24 0.14190613326655396 25 0.044867380371042806 26 0.03338967841565976 27 0.037563388217617226 28 0.029215968613702292 29 0.04069367056908533 30 0.07095306663327698 31 0.042780525470064064 32 0.041737098019574696 33 0.044867380371042806 34 0.04695423527202154 35 0.045910807821532174 36 0.051127945073979 37 0.03651996076712786 38 0.04695423527202154 39 0.04904109017300027 40 0.19407750579102234 41 0.04904109017300027 42 0.05008451762348964 43 0.04069367056908533 44 0.02608568626223419 45 0.029215968613702292 46 0.018781694108808613 47 0.02191197646027672 48 0.017738266658319245 49 0.015651411757340514 50 0.02191197646027672 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 95838.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.90577850122081 #Duplication Level Percentage of deduplicated Percentage of total 1 96.29479427813007 81.75984473799537 2 1.0163201101115862 1.7258290031094137 3 0.525979452391486 1.339760846428348 4 0.35761687066804304 1.2145495523696237 5 0.3195202280882859 1.3564556856361776 6 0.24332694292877158 1.2395918111813686 7 0.24332694292877158 1.4461904463782633 8 0.2015435284864573 1.36897681504205 9 0.16221796195251437 1.2395918111813686 >10 0.6304379884972718 6.921054279095975 >50 0.0036867718625571448 0.23685803126108643 >100 0.001228923954185715 0.15129698032095829 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG 145 0.15129698032095829 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.011477701955383042 0.0 2 0.0 0.0 0.0 0.014607984306851144 0.0 3 0.0 0.0 0.0 0.014607984306851144 0.0 4 0.0 0.0 0.0 0.017738266658319245 0.0 5 0.0 0.0010434274504893676 0.0 0.022955403910766083 0.0 6 0.0 0.0010434274504893676 0.0 0.022955403910766083 0.0 7 0.0 0.0010434274504893676 0.0 0.022955403910766083 0.0 8 0.0 0.0010434274504893676 0.0 0.022955403910766083 0.0 9 0.0 0.0010434274504893676 0.0 0.02399883136125545 0.0 10 0.0 0.0010434274504893676 0.0 0.026085686262234186 0.0 11 0.0 0.0010434274504893676 0.0 0.02817254116321292 0.0 12 0.0 0.0010434274504893676 0.0 0.02817254116321292 0.0 13 0.0 0.0010434274504893676 0.0 0.02817254116321292 0.0 14 0.0 0.0010434274504893676 0.0 0.02921596861370229 0.0 15 0.0 0.0010434274504893676 0.0 0.03338967841565976 0.0 16 0.0 0.0010434274504893676 0.0 0.03547653331663849 0.0 17 0.0 0.0010434274504893676 0.0010434274504893676 0.04904109017300027 0.0 18 0.0 0.0010434274504893676 0.0010434274504893676 0.05425822742544711 0.0 19 0.0 0.0010434274504893676 0.0010434274504893676 0.06990963918278761 0.0 20 0.0 0.0010434274504893676 0.0010434274504893676 0.07408334898474508 0.0 21 0.0 0.0010434274504893676 0.0010434274504893676 0.07721363133621319 0.0 22 0.0 0.0010434274504893676 0.0010434274504893676 0.0959953254450218 0.0 23 0.0 0.0010434274504893676 0.0010434274504893676 0.09703875289551117 0.0 24 0.0 0.0010434274504893676 0.0010434274504893676 0.10329931759844738 0.0 25 0.0 0.0010434274504893676 0.0010434274504893676 0.10642959994991548 0.0 26 0.0 0.0010434274504893676 0.0010434274504893676 0.11060330975187295 0.0 27 0.0 0.0010434274504893676 0.0010434274504893676 0.13251528621214967 0.0 28 0.0 0.0010434274504893676 0.0010434274504893676 0.14712327051900081 0.0 29 0.0 0.0010434274504893676 0.0010434274504893676 0.15860097247438384 0.0 30 0.0 0.0010434274504893676 0.0010434274504893676 0.17529581168221373 0.0 31 0.0 0.0010434274504893676 0.0010434274504893676 0.20346835284542666 0.0 32 0.0 0.0010434274504893676 0.0010434274504893676 0.22538032930570337 0.0 33 0.0 0.0010434274504893676 0.0010434274504893676 0.29215968613702287 0.0 34 0.0 0.0010434274504893676 0.0010434274504893676 0.3912852939335128 0.0 35 0.0 0.0010434274504893676 0.0010434274504893676 0.4841503370270665 0.0 36 0.0 0.0010434274504893676 0.0010434274504893676 0.6354473173480248 0.0 37 0.0 0.0010434274504893676 0.0010434274504893676 0.834741960391494 0.0 38 0.0 0.0010434274504893676 0.0010434274504893676 1.0778605563555166 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGACAA 20 7.7687483E-4 44.0591 21 CTTTACA 95 0.0 41.696583 1 TTTACAC 100 0.0 37.37205 2 CTTATAG 25 0.0023386104 35.21045 1 ACGATAA 25 0.002344617 35.192062 24 GACGATA 25 0.002344617 35.192062 23 TTACACA 120 0.0 34.807304 3 CGACGAT 40 1.8054998E-5 32.99256 22 CTATACA 95 0.0 32.430676 1 CTTTATA 35 3.2100067E-4 31.405087 2 GCTGCCT 30 0.0056815343 29.372734 17 AGATCCC 30 0.005725278 29.326717 40 AAGCACG 30 0.005739918 29.311415 30 CCAAAAT 30 0.005739918 29.311415 32 CTTATAC 695 0.0 28.181025 1 TTATACA 710 0.0 27.866484 2 TCTTTAT 40 6.9724966E-4 27.508165 1 GACGAAT 40 6.9938437E-4 27.493801 23 CTGACGG 45 0.0013780103 24.477278 12 CGAATGA 45 0.0013906705 24.438932 25 >>END_MODULE