FastQCFastQC Report
Wed 25 May 2016
SRR1780515_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780515_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95838
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT2758828.78607650410067No Hit
ATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTT2213823.099396898933616RNA PCR Primer, Index 45 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC66026.888708028130804No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG35583.7125148688411693No Hit
CTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC35313.6843423276779563No Hit
TATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCT22422.339364343997162No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC17471.822867756004925No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC11931.2448089484338154No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG9671.0089943446232184No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC8050.8399590976439408No Hit
CTTATAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG6460.6740541330161314No Hit
CTTAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC5140.5363217095515349No Hit
TTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC5060.52797428994762No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG4290.4476303762599386No Hit
CTTATTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG4240.44241323900749185No Hit
CTTATCACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC3260.3401573488595338No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTATCTCGTATGCCGTC2710.2827688390826186No Hit
ACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTTCT2310.2410317410630439RNA PCR Primer, Index 45 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTCTTATTATCTCGTATGCCGTCTTC2200.22955403910766087RNA PCR Primer, Index 35 (95% over 22bp)
CTTATATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC1920.20033807049395855No Hit
CTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCT1880.19616436069200108No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATATCGTATGCCGT1850.193034078340533No Hit
TCTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT1630.1700786744297669No Hit
ATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTTCTGCTT1610.16799181952878817RNA PCR Primer, Index 29 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACTCTTATTAATATCGTATGCCGTCTT1560.16277468227634131No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG1460.15234040777144764No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGC1240.12938500386068158No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATAATCTCGTATGCCGTC1130.11790730190529852RNA PCR Primer, Index 12 (95% over 21bp)
TCTTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT1090.11373359210334105No Hit
CTCTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG980.102255890147958No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACAC207.820357E-444.04
TCTTTAT1200.044.01
CCGTCTT21800.043.09174344
CTTTACA7750.042.5806471
TTTACAC7850.042.0382162
CTATACA4550.041.582421
TCTTAAT750.038.133331
CTTATAA357.2205676E-637.7142871
TCACATC357.2205676E-637.7142875
GCCGTCT25000.037.57599643
TTACACA8900.037.078653
CTTATAC37350.036.1070981
CTTACAC553.3560354E-936.01
TATAACA250.002342030435.23
TTATTAC505.8489604E-835.22
TTATAAC250.002342030435.22
TATTACA505.8489604E-835.23
TTATACA39200.034.2346952
TTATCAC451.0240383E-634.222222
ATTACAC551.3514364E-732.04
Produced by FastQC (version 0.11.5)