FastQCFastQC Report
Wed 25 May 2016
SRR1780514_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780514_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51590
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGAAG200.006916512352.4963611
GTCGCCG200.006916512352.4963653
TGCTTGA200.006916512352.4963655
TGGTTTT200.006916512352.4963627
GTGTGAC200.006916512352.4963611
TCGACAT200.006916512352.4963669
TTCGACA200.006916512352.4963668
ATGGTTT350.001245070239.99722726
GAGACAA400.002398948434.9975742
TTTACAC450.00427220831.1089572
CCGCCTA450.00427220831.10895715
CTATCCT500.007150074527.9980640
ATGGGAA900.005212225519.44309870
ACACCTA900.005212225519.4430985
TTACACA900.005212225519.4430983
GATGGGA950.00712273518.41977569
CGATGGG1200.001318279417.49878768
CCTAACC1250.001732773816.7988363
ACGATGG1300.002252211316.15272767
GACGATG1450.00466111614.48175566
Produced by FastQC (version 0.11.5)