FastQCFastQC Report
Wed 25 May 2016
SRR1780514_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780514_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51590
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT33846.559410738515216No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT30175.848032564450475RNA PCR Primer, Index 27 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC11092.1496414033727467No Hit
CTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC5761.1164954448536537No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG3930.7617755378949409No Hit
TATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT2710.5252955999224657No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC1740.3372746656328746No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG1660.3217677844543516No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC1590.308199263423144No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC1430.2771855010660981No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGT1320.255863539445629No Hit
CTTAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC1050.20352781546811397No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGTCTT1030.19965109517348323No Hit
CTTATAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG1020.19771273502616785No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGT860.16669897266912193No Hit
TTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC830.1608838922271758No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGT760.14731537119596821No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGTCTT710.13762357045939136No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGTCTT640.12405504942818377No Hit
CTTATTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG550.10660980810234541No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG150.00221283670.0000169
TAGGAGT150.00221283670.000014
TAGAGGA207.857113E-570.09
TGGAATT207.857113E-570.023
TTTACAC1700.059.7058832
AGAGGAG308.267576E-658.33333610
CTTTACA1800.056.3888851
CTATACA750.056.01
CTTATAA252.3696036E-455.9999961
TCTGCCG200.00691461752.540
GATAAGT200.00691461752.542
AGGAGAC200.00691461752.512
GATCCTA200.00691461752.528
TGAACTG200.00691461752.528
ATAGAGG200.00691461752.58
GACCCTT200.00691461752.535
GATGGAA200.00691461752.521
TACTGGA200.00691461752.539
TGGGGTA200.00691461752.570
TGGATGG200.00691461752.519
Produced by FastQC (version 0.11.5)