##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780514_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51590 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.946695095948826 34.0 34.0 34.0 32.0 34.0 2 33.30641597208761 34.0 34.0 34.0 33.0 34.0 3 33.47846481876333 34.0 34.0 34.0 34.0 34.0 4 33.53271951928669 34.0 34.0 34.0 34.0 34.0 5 33.528106222136074 34.0 34.0 34.0 34.0 34.0 6 37.2810816049622 38.0 38.0 38.0 36.0 38.0 7 36.86460554371002 38.0 37.0 38.0 35.0 38.0 8 36.934754797441364 38.0 37.0 38.0 35.0 38.0 9 36.689881760031014 38.0 37.0 38.0 34.0 38.0 10-11 36.442052723396 38.0 37.0 38.0 34.0 38.0 12-13 36.25234541577825 38.0 37.0 38.0 34.0 38.0 14-15 36.38479356464431 38.0 37.0 38.0 34.0 38.0 16-17 36.468666408218645 38.0 37.0 38.0 34.0 38.0 18-19 36.56307423919364 38.0 37.0 38.0 34.0 38.0 20-21 36.31002132196162 38.0 37.0 38.0 34.0 38.0 22-23 36.34685985656135 38.0 37.0 38.0 33.5 38.0 24-25 35.513655747237834 38.0 36.0 38.0 30.0 38.0 26-27 35.76070943981392 38.0 36.0 38.0 31.5 38.0 28-29 35.656474122892035 38.0 36.0 38.0 31.0 38.0 30-31 35.29363248691607 38.0 36.0 38.0 29.0 38.0 32-33 35.80087226206629 38.0 36.5 38.0 32.0 38.0 34-35 35.55773405698778 38.0 36.0 38.0 31.0 38.0 36-37 35.52139949602636 38.0 36.0 38.0 31.0 38.0 38-39 34.86261872455903 37.5 35.0 38.0 26.5 38.0 40-41 34.92484977708858 37.0 35.0 38.0 29.0 38.0 42-43 35.6283775925567 37.5 36.5 38.0 32.0 38.0 44-45 35.862492731149445 38.0 36.5 38.0 32.5 38.0 46-47 36.04744136460555 38.0 37.0 38.0 33.5 38.0 48-49 35.76340376041868 38.0 36.5 38.0 31.5 38.0 50-51 35.828561736770695 38.0 37.0 38.0 31.5 38.0 52-53 35.69585190928474 38.0 36.0 38.0 31.0 38.0 54-55 35.90162822252375 38.0 37.0 38.0 32.5 38.0 56-57 35.90698778833107 38.0 37.0 38.0 32.5 38.0 58-59 35.8659527040124 38.0 37.0 38.0 32.0 38.0 60-61 35.65773405698779 38.0 36.5 38.0 31.5 38.0 62-63 36.08427989920527 38.0 37.0 38.0 33.5 38.0 64-65 36.062512114750916 38.0 37.0 38.0 33.0 38.0 66-67 35.04372940492344 38.0 35.5 38.0 29.0 38.0 68-69 33.434396200814106 37.0 33.5 38.0 21.5 38.0 70-71 32.283562705950764 37.0 29.5 38.0 16.0 38.0 72-73 31.112502422950183 37.0 25.0 38.0 11.0 38.0 74-75 31.381585578600507 37.0 26.0 38.0 11.0 38.0 76 31.066795890676488 37.0 25.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3189687241114427 1101 2 0.4258000161989983 1101 3 -0.11493978096724788 1101 4 0.21571667364681701 1101 5 0.25706968463910584 1101 6 -1.9511109588256659 1101 7 0.4372482613394908 1101 8 0.5780574205917759 1101 9 0.3228530006616097 1101 10-11 0.28884379267780247 1101 12-13 0.6782532954526488 1101 14-15 0.5862454296595203 1101 16-17 0.0068858426477120815 1101 18-19 -0.26862689882338486 1101 20-21 0.02462146081042249 1101 22-23 0.814034163501816 1101 24-25 -1.5244759193254396 1101 26-27 -0.05190673676644053 1101 28-29 0.7833713232285859 1101 30-31 0.892633972545724 1101 32-33 0.1712115255261395 1101 34-35 0.3619292194922963 1101 36-37 0.043483849621217985 1101 38-39 -0.12006689337619747 1101 40-41 0.7843277321284248 1101 42-43 0.550904158123771 1101 44-45 0.4772374238927668 1101 46-47 0.8922750819419178 1101 48-49 -0.22044991856414242 1101 50-51 -1.393153770645256 1101 52-53 -1.7270644587329826 1101 54-55 -0.6847335641955254 1101 56-57 -0.37506080226668814 1101 58-59 -0.6354515349068635 1101 60-61 -1.744471573899446 1101 62-63 -1.0680424780210345 1101 64-65 -1.292399940458921 1101 66-67 -1.412936229447702 1101 68-69 -0.32022916144894253 1101 70-71 -0.740287226565048 1101 72-73 -1.6908920570948212 1101 74-75 -0.40013664463655374 1101 76 -0.678261934422693 1102 1 -1.0579924890702443 1102 2 0.05321325621066819 1102 3 0.38304797056380835 1102 4 0.3348184525911151 1102 5 0.030298031095803424 1102 6 0.34040260197922834 1102 7 9.694628559628882E-4 1102 8 0.25790577288107386 1102 9 0.5885864506470213 1102 10-11 0.45513134006771594 1102 12-13 0.0798864246448332 1102 14-15 0.2228158583559363 1102 16-17 -0.027847315707489884 1102 18-19 -0.7847540495962093 1102 20-21 0.36404986559315944 1102 22-23 0.47527650820872935 1102 24-25 -6.257809252658774 1102 26-27 -1.9124200059411294 1102 28-29 -0.10691739068217032 1102 30-31 1.5615170288303588 1102 32-33 0.3174932403117907 1102 34-35 0.28164342188366476 1102 36-37 -2.9824420763047073 1102 38-39 -0.38632527774481673 1102 40-41 0.4127325148639187 1102 42-43 0.43576855007477633 1102 44-45 0.9068962165436787 1102 46-47 0.809889546800477 1102 48-49 -3.905897570940926 1102 50-51 -0.9776390433195061 1102 52-53 0.005364820940393145 1102 54-55 -0.024424436169866226 1102 56-57 0.7265431639041964 1102 58-59 -0.502030712509665 1102 60-61 -5.903001757626484 1102 62-63 -2.796068141479765 1102 64-65 0.045657802323226804 1102 66-67 1.1557718487092004 1102 68-69 -1.209817962848767 1102 70-71 -2.1224977835787584 1102 72-73 -5.2412857578822205 1102 74-75 -2.653359158488989 1102 76 -0.7683173441631403 1103 1 0.6599294597241894 1103 2 0.46390179710054724 1103 3 0.21645587511265774 1103 4 0.3883108711842027 1103 5 0.2423785680085544 1103 6 0.42958977573016455 1103 7 0.746184476527354 1103 8 0.6880822580911143 1103 9 0.6896553047360143 1103 10-11 0.5672615911241436 1103 12-13 -0.19351650848964397 1103 14-15 -0.12297086796770174 1103 16-17 0.5940020504371759 1103 18-19 0.7658549486637938 1103 20-21 0.3007140465526419 1103 22-23 0.5773645022430358 1103 24-25 -2.943939006573764 1103 26-27 -0.27734667757921017 1103 28-29 0.360420416924093 1103 30-31 1.1095657486911605 1103 32-33 1.451795999446766 1103 34-35 0.3137339016251488 1103 36-37 -0.8524893047411979 1103 38-39 0.006789313356350135 1103 40-41 0.5983899844140055 1103 42-43 0.2271182890755199 1103 44-45 1.1509678049105716 1103 46-47 1.1442171630072906 1103 48-49 -1.7202649584626784 1103 50-51 -0.20013469333193257 1103 52-53 0.638971582063931 1103 54-55 0.25747961553284426 1103 56-57 1.0156806235114573 1103 58-59 0.18104162156937775 1103 60-61 -3.8609435920783852 1103 62-63 -0.8517976965381848 1103 64-65 0.22440500590264634 1103 66-67 -0.050467276321874976 1103 68-69 0.6914497478149215 1103 70-71 0.007057274835361227 1103 72-73 -3.61175555653994 1103 74-75 -0.8909679081782755 1103 76 -1.8221080476182756 1104 1 0.0898404502520549 1104 2 -0.028268225270814185 1104 3 0.26200778143851267 1104 4 0.07130387418291662 1104 5 -0.3143828199680243 1104 6 0.1862654861399875 1104 7 0.6211428277101803 1104 8 0.6249664505800467 1104 9 1.0730624159740572 1104 10-11 -0.44494746213874947 1104 12-13 0.6526208612090585 1104 14-15 0.901066449372486 1104 16-17 0.28468223511993784 1104 18-19 0.7194224673462699 1104 20-21 -0.06888945670786484 1104 22-23 0.7641653970976208 1104 24-25 0.7024744352844863 1104 26-27 1.5514885834993706 1104 28-29 1.6320897015164135 1104 30-31 0.5523759728807889 1104 32-33 1.036878583101391 1104 34-35 -0.016386522954789484 1104 36-37 1.199512218415542 1104 38-39 1.7370001753678679 1104 40-41 2.113835745754379 1104 42-43 0.9927425453466441 1104 44-45 0.8132791952670928 1104 46-47 1.1061858430967817 1104 48-49 0.8119117277194334 1104 50-51 0.6683026430477099 1104 52-53 0.7598959478119482 1104 54-55 0.040429779748180295 1104 56-57 0.14624371551964543 1104 58-59 0.2272837082941237 1104 60-61 0.7948705827990494 1104 62-63 0.7192194865738202 1104 64-65 1.0045230505501763 1104 66-67 -0.1914306729614026 1104 68-69 2.1183144248580916 1104 70-71 1.2097323188641127 1104 72-73 0.6679341002738042 1104 74-75 -0.1799155020666099 1104 76 -0.1695781794665301 1105 1 0.7894149183371582 1105 2 0.271731774729183 1105 3 0.2719368594527012 1105 4 -0.391107473334813 1105 5 -0.3897019689041983 1105 6 0.21151371309034062 1105 7 0.6550435369300374 1105 8 -0.7428349678596717 1105 9 0.3937716358322092 1105 10-11 0.2032794882158626 1105 12-13 -0.8341876494292393 1105 14-15 0.005038080578152915 1105 16-17 0.12993046207029124 1105 18-19 0.7500607652186133 1105 20-21 0.6933091248524192 1105 22-23 0.27083206376428137 1105 24-25 0.8088574140078961 1105 26-27 0.24943184591072054 1105 28-29 0.5030826093178291 1105 30-31 0.8833688806822053 1105 32-33 0.8597154625340124 1105 34-35 -1.0820602818200413 1105 36-37 0.456817182954552 1105 38-39 0.4118228704033342 1105 40-41 0.8353251074565193 1105 42-43 -0.018675894369664547 1105 44-45 0.30022954987701667 1105 46-47 0.8361858430967786 1105 48-49 -0.5007123857557403 1105 50-51 0.7486572529767912 1105 52-53 0.5301796357552107 1105 54-55 1.0163163045708785 1105 56-57 0.5528394602005022 1105 58-59 0.5135248430458859 1105 60-61 0.9069982423735183 1105 62-63 -0.7068088822205141 1105 64-65 0.8189911356565602 1105 66-67 0.4298459227832794 1105 68-69 0.2512931482623415 1105 70-71 0.9482429571619875 1105 72-73 0.6053809087844471 1105 74-75 0.3092334341036036 1105 76 0.728719692873895 1106 1 -1.3437223365647988 1106 2 -0.11160155860414989 1106 3 0.4062505849428959 1106 4 0.11526507568479616 1106 5 -0.08440785125714001 1106 6 0.13729802681583436 1106 7 -0.689662345422903 1106 8 0.5872630713560199 1106 9 -0.11201267789328284 1106 10-11 0.5765638019413544 1106 12-13 1.0844888211589918 1106 14-15 -0.28559917432379933 1106 16-17 0.5053226189330289 1106 18-19 0.4408450789441005 1106 20-21 0.43997579151908894 1106 22-23 0.8548516716074204 1106 24-25 0.3836123159686764 1106 26-27 0.9159024341460125 1106 28-29 0.5538669230433229 1106 30-31 0.6543002532312272 1106 32-33 0.34559781547518753 1106 34-35 0.8968122671995715 1106 36-37 1.010395614327102 1106 38-39 0.022852282168038585 1106 40-41 0.9621878525545569 1106 42-43 0.6999025370028846 1106 44-45 1.1578766087005476 1106 46-47 0.9284897646654002 1106 48-49 1.4773268299305329 1106 50-51 0.30615725297678864 1106 52-53 0.6988561063434418 1106 54-55 0.002100618296367429 1106 56-57 0.38362377392599 1106 58-59 1.013083666575298 1106 60-61 0.7420962815892054 1106 62-63 1.2641715099363537 1106 64-65 -0.8450284721865771 1106 66-67 0.8053361188617103 1106 68-69 1.667959814929013 1106 70-71 -0.6202374349948769 1106 72-73 -1.6809916402351632 1106 74-75 -1.206354801190514 1106 76 -0.7861822679104193 1107 1 0.4138293327515754 1107 2 -0.07781777482036745 1107 3 0.24806298557882656 1107 4 -0.031197563424903763 1107 5 -0.11699926620149625 1107 6 -0.058846647270016206 1107 7 0.37909759098409523 1107 8 -0.16571785074255274 1107 9 -0.49694908488851297 1107 10-11 0.28413534407172136 1107 12-13 -0.3989173791589664 1107 14-15 -0.29325020771013044 1107 16-17 0.46975028189010715 1107 18-19 0.17713283729067797 1107 20-21 0.5278136293569275 1107 22-23 -0.3315553236230997 1107 24-25 -0.29136781121732724 1107 26-27 -0.4294870730081968 1107 28-29 0.33745197868719856 1107 30-31 -0.0023968850835629496 1107 32-33 0.8694451922637398 1107 34-35 0.16082260106283996 1107 36-37 0.6718622279995969 1107 38-39 0.17060665418711096 1107 40-41 -0.026882099750693556 1107 42-43 0.4255583398645655 1107 44-45 0.4794187390662117 1107 46-47 0.7942489061598366 1107 48-49 0.8477560827127206 1107 50-51 0.6693329286524659 1107 52-53 0.47896341953899224 1107 54-55 0.9985235117780817 1107 56-57 0.8766682890293254 1107 58-59 0.46906538358643246 1107 60-61 0.13100725138252756 1107 62-63 0.5992271538155265 1107 64-65 0.655567712233136 1107 66-67 -0.5034874105500577 1107 68-69 -0.1698780229088257 1107 70-71 -0.5684687545497269 1107 72-73 0.3776331610366981 1107 74-75 -0.8979287280585595 1107 76 0.05205302620722918 1108 1 0.1786386626293961 1108 2 0.28908337290270225 1108 3 0.09339804666731055 1108 4 0.17418933031815698 1108 5 0.14262679821909074 1108 6 -0.4462031818868297 1108 7 -0.3016687918370877 1108 8 -0.9632002646633211 1108 9 -0.37097722261528077 1108 10-11 -0.006081242377746321 1108 12-13 0.8017027615296612 1108 14-15 0.3763166193909413 1108 16-17 -0.5989279854182925 1108 18-19 -0.007382157155824132 1108 20-21 0.8132634627519622 1108 22-23 -0.2997615435416563 1108 24-25 1.0816884642362083 1108 26-27 -0.6989243184728409 1108 28-29 -0.5895657925086581 1108 30-31 0.24510404049955525 1108 32-33 0.42738669541073193 1108 34-35 0.3076200834767704 1108 36-37 0.9955386441417673 1108 38-39 0.693329719718399 1108 40-41 0.5324940572281989 1108 42-43 0.18333323805042312 1108 44-45 0.4751610567263356 1108 46-47 0.6141767106766878 1108 48-49 1.1098812215501965 1108 50-51 0.06728738996308437 1108 52-53 -0.8716011861626001 1108 54-55 -1.0608526452008107 1108 56-57 -0.18789113340680785 1108 58-59 -0.13483132133767128 1108 60-61 0.8645781510493151 1108 62-63 1.0437847250854304 1108 64-65 0.6246532361131827 1108 66-67 0.7054166990389845 1108 68-69 0.561795431367365 1108 70-71 -1.2979670885001156 1108 72-73 -1.0138884976082494 1108 74-75 -1.2785747850744826 1108 76 -1.095207247765373 1109 1 -1.3397869595971095 1109 2 0.4271308357620498 1109 3 0.3766316951334545 1109 4 0.29950285530368603 1109 5 0.08522760856059364 1109 6 -0.2606928596796081 1109 7 -0.19256209687277703 1109 8 0.40261104153000105 1109 9 0.7296401804331509 1109 10-11 0.21290390136140047 1109 12-13 0.45933347733132024 1109 14-15 0.6347094420804993 1109 16-17 0.6980525277979908 1109 18-19 0.6220795445613305 1109 20-21 0.9294124112373936 1109 22-23 -0.11024774844229057 1109 24-25 -0.12306746862121543 1109 26-27 -0.017610407610398227 1109 28-29 -1.3353211465507115 1109 30-31 0.68707780087 1109 32-33 0.7136591245058455 1109 34-35 -0.25961896726604294 1109 36-37 0.976582441170514 1109 38-39 0.9378792084315037 1109 40-41 0.26894013093069447 1109 42-43 1.0833428849730637 1109 44-45 0.5789619442432183 1109 46-47 0.6824769229089824 1109 48-49 0.34290263771843854 1109 50-51 -0.9165540146288436 1109 52-53 -0.8523555755123979 1109 54-55 -0.37124238087513106 1109 56-57 -0.2555642956680515 1109 58-59 -0.1929540301935475 1109 60-61 0.8303315757068503 1109 62-63 0.4035667046339526 1109 64-65 0.8383808070180621 1109 66-67 -0.042219804916250325 1109 68-69 -1.039786663944227 1109 70-71 -0.9418509395516281 1109 72-73 -0.664525194501941 1109 74-75 -1.293865157445694 1109 76 -1.0430173963279827 1110 1 -1.262575489336701 1110 2 -0.34001882479120127 1110 3 0.21486251892512342 1110 4 -0.020843684365985382 1110 5 0.2864131390094684 1110 6 -0.05160379290486361 1110 7 0.7513025297357956 1110 8 0.7915055597182672 1110 9 0.8754502929065495 1110 10-11 -0.2530754278512788 1110 12-13 0.8065317750311962 1110 14-15 1.0747742916093372 1110 16-17 0.4796666731014625 1110 18-19 -0.0816898343017769 1110 20-21 1.1180333454759221 1110 22-23 -0.28379623359783324 1110 24-25 -0.8014543365916253 1110 26-27 1.2897915581409407 1110 28-29 0.5798931608765869 1110 30-31 0.6404672020251283 1110 32-33 0.6253269733253859 1110 34-35 1.1676279675804437 1110 36-37 1.1046349287579105 1110 38-39 1.438081863209085 1110 40-41 -1.1311257318720251 1110 42-43 0.22489724711714132 1110 44-45 1.1373518520352874 1110 46-47 0.9359220541279498 1110 48-49 1.647872746138745 1110 50-51 0.41721840405593014 1110 52-53 0.6655033767624019 1110 54-55 -0.4314055179710934 1110 56-57 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-0.05977045012297566 2116 46-47 -0.7066346697237336 2116 48-49 -0.21045597549932893 2116 50-51 -1.3967273624078302 2116 52-53 0.20864117421675132 2116 54-55 -1.0054785672239959 2116 56-57 -0.3335707962097558 2116 58-59 -2.561859772338721 2116 60-61 -1.454283808908535 2116 62-63 0.234985989574362 2116 64-65 -2.222290915625493 2116 66-67 -1.3842566413192898 2116 68-69 -1.1166555696863725 2116 70-71 0.8387557776748054 2116 72-73 -0.26051652711299056 2116 74-75 -0.49922810435793963 2116 76 -1.1825623584081555 2117 1 0.4064892588647382 2117 2 0.4873193047052027 2117 3 0.4306898570546167 2117 4 0.07987574009444387 2117 5 0.07058580087997512 2117 6 0.35846814954118145 2117 7 0.6937485729012636 2117 8 0.5612897323801391 2117 9 -2.0741899948632323 2117 10-11 -0.49767974439804874 2117 12-13 -0.6898711026666504 2117 14-15 -0.13745592421800268 2117 16-17 -0.8872398017186853 2117 18-19 -1.3766538630787508 2117 20-21 0.1827815469147751 2117 22-23 -0.31617033431725616 2117 24-25 -1.650375199900978 2117 26-27 -2.4979782260317265 2117 28-29 -1.1431284218572344 2117 30-31 0.5505391444711805 2117 32-33 -1.3027305806314544 2117 34-35 0.9553977517531038 2117 36-37 -0.8306168698032437 2117 38-39 -0.07630662599954974 2117 40-41 0.33290304510639146 2117 42-43 0.22489724711714132 2117 44-45 -0.6324323206265774 2117 46-47 -0.43458154299435137 2117 48-49 -0.737019340192191 2117 50-51 -0.5324218837138588 2117 52-53 0.9712587724458572 2117 54-55 -0.3450745827192918 2117 56-57 0.36288742183118927 2117 58-59 -0.8853240778174154 2117 60-61 -1.0797403667391876 2117 62-63 -0.6898064841389697 2117 64-65 -1.1380352432403242 2117 66-67 -0.661760791845019 2117 68-69 0.5096864336340516 2117 70-71 -0.2143709517109187 2117 72-73 0.5414480550674199 2117 74-75 0.27592408158561454 2117 76 1.2696789254878063 2118 1 -0.6294819641568452 2118 2 0.07005311765484379 2118 3 -1.1952094235209216 2118 4 0.14462394153328972 2118 5 0.3223843620310518 2118 6 0.17861203443326445 2118 7 0.7728852635487442 2118 8 0.41740484029380553 2118 9 -2.1749094193236687 2118 10-11 -0.6451617587865357 2118 12-13 -1.0819574336019073 2118 14-15 0.4704577448467404 2118 16-17 -2.592275772941697 2118 18-19 -0.3442797623593279 2118 20-21 -0.47549183438019327 2118 22-23 0.4859879390640387 2118 24-25 0.3604161670054964 2118 26-27 -2.7605681540892775 2118 28-29 -0.1071571988356439 2118 30-31 -0.9458637332266662 2118 32-33 -0.6084859763149097 2118 34-35 -0.7784151978871847 2118 36-37 -3.161552121601801 2118 38-39 -0.2741483526182549 2118 40-41 -0.14191709877849945 2118 42-43 0.3256166715775777 2118 44-45 -2.1827920328567885 2118 46-47 -0.07846643508068496 2118 48-49 0.22341231448406518 2118 50-51 -0.5899758405483908 2118 52-53 -1.1186692851080977 2118 54-55 -0.7659378920718112 2118 56-57 0.16144857291033077 2118 58-59 -0.8097845094720952 2118 60-61 -1.0149921653003418 2118 62-63 -1.822900009318829 2118 64-65 0.06340360568054138 2118 66-67 0.5972320139103786 2118 68-69 -1.8176516958623523 2118 70-71 0.10217581087901095 2118 72-73 0.6529588464343234 2118 74-75 0.013334153528063553 2118 76 -0.18355848458413604 2119 1 0.8107482516704962 2119 2 -1.2336015586041498 2119 3 -0.005396473880630026 2119 4 -0.052440806668144546 2119 5 -0.20970196890419857 2119 6 -0.6338196202429884 2119 7 -0.90095646306996 2119 8 0.28249836547366414 2119 9 0.2991049691655405 2119 10-11 -0.4100538451174671 2119 12-13 -0.42218764942924025 2119 14-15 -0.3616285860885071 2119 16-17 -0.452736204596377 2119 18-19 -0.19927256811472915 2119 20-21 -1.8420242084809075 2119 22-23 -0.018501269569050294 2119 24-25 0.9675240806745578 2119 26-27 0.1954318459107256 2119 28-29 -0.06025072401549636 2119 30-31 0.9080355473488666 2119 32-33 0.40771546253401425 2119 34-35 0.48460638484662866 2119 36-37 0.21148384962121725 2119 38-39 -2.2221771295966732 2119 40-41 -0.7453415592101535 2119 42-43 0.1739907722970031 2119 44-45 0.05222954987701911 2119 46-47 0.06351917643010552 2119 48-49 -0.20737905242241084 2119 50-51 0.8426572529767853 2119 52-53 0.17617963575521145 2119 54-55 -0.13301702876245258 2119 56-57 -1.5064938731328397 2119 58-59 -0.13447515695411028 2119 60-61 0.35433157570685125 2119 62-63 -0.13147554888717394 2119 64-65 0.44032446898989264 2119 66-67 0.5045125894499378 2119 68-69 1.0519598149290132 2119 70-71 -0.31509037617134794 2119 72-73 0.8827142421177783 2119 74-75 0.1632334341036028 2119 76 0.5300530262072307 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 0.0 20 3.0 21 2.0 22 5.0 23 24.0 24 40.0 25 112.0 26 172.0 27 345.0 28 560.0 29 870.0 30 1295.0 31 1917.0 32 2984.0 33 4349.0 34 6104.0 35 7486.0 36 9301.0 37 16019.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.45532079860438 24.03178910641597 11.294824578406667 35.21806551657298 2 14.64818763326226 17.003295212250435 47.79414615235511 20.554371002132196 3 14.318666408218647 27.730180267493697 37.71661174646249 20.234541577825162 4 9.89532855204497 24.60166698972669 35.11727078891258 30.385733669315755 5 9.937972475285909 40.676487691413065 36.31130063965885 13.074239193642178 6 20.308199263423145 38.96879240162822 16.030238418298122 24.692769916650516 7 20.28106222136073 37.10215157976352 18.247722426826904 24.369063772048847 8 17.735995347935646 36.216320992440394 23.556890870323706 22.49079278930025 9 16.803644117076953 20.690056212444272 17.14479550300446 45.361504167474315 10-11 17.452025586353944 25.48459003682884 31.754215933320413 25.309168443496798 12-13 21.6330684241132 18.386315177359954 28.537507268850554 31.443109129676294 14-15 22.900755960457452 22.55475867416166 19.748013180849004 34.796472184531886 16-17 24.960263616980036 27.85229695677457 18.94940880015507 28.238030626090328 18-19 22.33281643729405 26.41209536731925 19.89048265167668 31.364605543710024 20-21 20.230664857530527 23.71777476255088 19.6976158170188 36.353944562899784 22-23 22.8561736770692 30.583446404341924 20.099825547586743 26.460554371002132 24-25 24.986431478968793 32.76022484977709 19.659817794146154 22.593525877107968 26-27 22.00523357239775 29.56580732700136 28.582089552238806 19.846869548362086 28-29 21.13878658654778 25.93041287071138 30.57278542353169 22.35801511920915 30-31 22.94339988369839 22.954060864508627 28.129482457840666 25.973056793952313 32-33 17.260127931769723 26.54196549718938 24.828455126962588 31.36945144407831 34-35 15.861601085481682 25.36150416747432 27.381275440976932 31.39561930606707 36-37 15.949796472184532 27.48400852878465 32.62647799961233 23.939716999418494 38-39 19.8740065904245 22.316340376041868 29.445628997867807 28.364024035665825 40-41 18.56755185113394 23.666408218647025 31.82012017832913 25.9459197518899 42-43 25.4031789106416 22.509207210699746 33.153711959682106 18.933901918976545 44-45 18.80693932932739 25.28009304128707 30.261678619887576 25.65128900949796 46-47 25.500096918007365 19.757704981585576 26.857918201201787 27.88427989920527 48-49 22.921108742004265 19.028881566195 28.707113781740645 29.34289591006009 50-51 16.27641015700717 19.705369257608062 34.775150222911414 29.24307036247335 52-53 18.305873231246366 19.792595464237255 40.060089164566776 21.841442139949603 54-55 16.928668346578796 19.722814498933904 35.4419461135879 27.906571040899397 56-57 23.842798992052725 19.80713316534212 30.698778833107195 25.65128900949796 58-59 17.605156037991858 28.24190734638496 34.16359759643341 19.989339019189764 60-61 19.617173870905212 38.42023647993798 23.376623376623375 18.585966272533437 62-63 14.115138592750535 46.27834851715449 20.863539445628998 18.74297344446598 64-65 13.990114363248694 47.21554564838147 19.979647218453188 18.81469276991665 66-67 13.780771467338631 46.54584221748401 19.4892421011824 20.18414421399496 68-69 13.951347160302385 43.72940492343477 20.160883892227176 22.158364024035667 70-71 14.45144407830975 40.85578600503973 20.96239581314208 23.73037410350843 72-73 15.040705563093622 36.90637720488466 21.631130063965884 26.421787168055825 74-75 15.31886024423338 36.14654002713704 21.852103120759836 26.682496607869744 76 15.135009401228944 36.35852604237337 21.98918374071992 26.51728081567776 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.5 17 3.0 18 7.0 19 9.5 20 12.0 21 16.0 22 21.5 23 33.0 24 54.0 25 69.0 26 85.5 27 135.0 28 174.5 29 181.0 30 247.0 31 349.5 32 493.5 33 601.0 34 712.5 35 902.5 36 1091.0 37 1201.0 38 1404.5 39 1958.5 40 3393.0 41 5290.0 42 6103.0 43 6013.5 44 5560.5 45 4651.0 46 4105.0 47 3768.5 48 3096.5 49 2559.5 50 2358.0 51 2208.5 52 1853.5 53 1447.0 54 1246.0 55 1160.0 56 950.5 57 737.0 58 647.0 59 508.0 60 306.5 61 189.5 62 135.0 63 103.0 64 55.5 65 29.0 66 11.0 67 4.0 68 2.5 69 1.0 70 0.5 71 0.0 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0019383601473153714 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 51590.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.15565031982943 #Duplication Level Percentage of deduplicated Percentage of total 1 64.68738771110313 27.916262841635977 2 17.427236794825728 15.04167474316728 3 7.716492993172835 9.990308199263422 4 3.772906934962271 6.512890094979647 5 2.2637441609773625 4.884667571234735 6 1.3205174272367948 3.4192672998643148 7 0.7366151634926339 2.225237449118046 8 0.5389867049946101 1.8608257414227565 9 0.3368666906216313 1.3083930994378756 >10 1.109414301113906 6.9354526070943985 >50 0.026949335249730505 0.8431866640821863 >100 0.04491555874955084 3.3882535375072687 >500 0.0044915558749550845 1.1164954448536537 >1k 0.013474667624865252 14.557084706338438 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT 3384 6.559410738515216 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT 3017 5.848032564450475 RNA PCR Primer, Index 27 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 1109 2.1496414033727467 No Hit CTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 576 1.1164954448536537 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 393 0.7617755378949409 No Hit TATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT 271 0.5252955999224657 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC 174 0.3372746656328746 No Hit CTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 166 0.3217677844543516 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC 159 0.308199263423144 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC 143 0.2771855010660981 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGT 132 0.255863539445629 No Hit CTTAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 105 0.20352781546811397 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGTCTT 103 0.19965109517348323 No Hit CTTATAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 102 0.19771273502616785 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGT 86 0.16669897266912193 No Hit TTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 83 0.1608838922271758 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGT 76 0.14731537119596821 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGTCTT 71 0.13762357045939136 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGTCTT 64 0.12405504942818377 No Hit CTTATTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 55 0.10660980810234541 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.01938360147315371 0.0 2 0.0 0.0 0.0 0.01938360147315371 0.0 3 0.0 0.0 0.0 0.01938360147315371 0.0 4 0.0 0.0 0.0 0.021321961620469083 0.0 5 0.0 0.0 0.0 0.027137042062415198 0.0 6 0.0 0.0 0.0 0.027137042062415198 0.0 7 0.0 0.0 0.0 0.027137042062415198 0.0 8 0.0 0.0 0.0 0.031013762357045938 0.0 9 0.0 0.0 0.0 0.03295212250436131 0.0 10 0.0 0.0 0.0 0.03876720294630742 0.0 11 0.0 0.0 0.0 0.03876720294630742 0.0 12 0.0 0.0 0.0 0.0407055630936228 0.0 13 0.0 0.0 0.0 0.042643923240938165 0.0 14 0.0 0.0 0.0 0.04652064353556891 0.0 15 0.0 0.0 0.0 0.04845900368288428 0.0 16 0.0 0.0 0.0 0.05039736383019965 0.0 17 0.0 0.0 0.0 0.05039736383019965 0.0 18 0.0 0.0 0.0 0.05815080441946113 0.0 19 0.0 0.0 0.0 0.06590424500872262 0.0 20 0.0 0.0 0.0 0.06590424500872262 0.0 21 0.0 0.0 0.0 0.07171932545066874 0.0 22 0.0 0.0 0.0 0.07365768559798411 0.0 23 0.0 0.0 0.0 0.07947276603993021 0.0 24 0.0 0.0 0.0 0.08334948633456096 0.0 25 0.0 0.0 0.0 0.08528784648187633 0.0 26 0.0 0.0 0.0 0.08916456677650708 0.0 27 0.0 0.0 0.0 0.11630160883892227 0.0 28 0.0 0.0 0.0 0.15119209149059895 0.0 29 0.0 0.0 0.0 0.18608257414227564 0.0 30 0.0 0.0 0.0 0.21903469664663694 0.0 31 0.0 0.0 0.0 0.25198681915099824 0.0 32 0.0 0.0 0.0 0.2888156619499903 0.0 33 0.0 0.0 0.0 0.32758286489629773 0.0 34 0.0 0.0 0.0 0.3682884279899205 0.0 35 0.0 0.0 0.0 0.4710215157976352 0.0 36 0.0 0.0 0.0 0.6067067261097112 0.0 37 0.0 0.0 0.0 0.7714673386315177 0.0 38 0.0 0.0 0.0 1.0622213607288233 0.0 39 0.0 0.0 0.0 1.3587904632680752 0.0 40 0.0 0.0 0.0 1.692188408606319 0.0 41 0.0 0.0 0.0 2.234929249854623 0.0 42 0.0 0.0 0.0 2.76797829036635 0.0 43 0.0 0.0 0.0 3.372746656328746 0.0 44 0.0 0.0 0.0 3.859275053304904 0.0 45 0.0 0.0 0.0 4.4388447373522 0.0 46 0.0 0.0 0.0 5.055243264198488 0.0 47 0.0 0.0 0.0 5.563093622795115 0.0 48 0.0 0.0 0.0 6.094204303159527 0.0 49 0.0 0.0 0.0 6.627253343671254 0.0 50 0.0 0.0 0.0 7.096336499321574 0.0 51 0.0 0.0 0.0 7.594495057181624 0.0 52 0.0 0.0 0.0 8.214770304322544 0.0 53 0.0 0.0 0.0 8.800155068811785 0.0 54 0.0 0.0 0.0 9.339019189765459 0.0 55 0.0 0.0 0.0 9.912773793370809 0.0 56 0.0 0.0 0.0 10.453576274471796 0.0 57 0.0 0.0 0.0 10.924597790269432 0.0 58 0.0 0.0 0.0 11.341345221942237 0.0 59 0.0 0.0 0.0 11.773599534793565 0.0 60 0.0 0.0 0.0 12.232990889707308 0.0 61 0.0 0.0 0.0 12.669121922853266 0.0 62 0.0 0.0 0.0 13.155650319829425 0.0 63 0.0 0.0 0.0 13.649932157394844 0.0 64 0.0 0.0 0.0 14.130645473929055 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAACACG 15 0.002212836 70.00001 69 TAGGAGT 15 0.002212836 70.00001 4 TAGAGGA 20 7.857113E-5 70.0 9 TGGAATT 20 7.857113E-5 70.0 23 TTTACAC 170 0.0 59.705883 2 AGAGGAG 30 8.267576E-6 58.333336 10 CTTTACA 180 0.0 56.388885 1 CTATACA 75 0.0 56.0 1 CTTATAA 25 2.3696036E-4 55.999996 1 TCTGCCG 20 0.006914617 52.5 40 GATAAGT 20 0.006914617 52.5 42 AGGAGAC 20 0.006914617 52.5 12 GATCCTA 20 0.006914617 52.5 28 TGAACTG 20 0.006914617 52.5 28 ATAGAGG 20 0.006914617 52.5 8 GACCCTT 20 0.006914617 52.5 35 GATGGAA 20 0.006914617 52.5 21 TACTGGA 20 0.006914617 52.5 39 TGGGGTA 20 0.006914617 52.5 70 TGGATGG 20 0.006914617 52.5 19 >>END_MODULE