FastQCFastQC Report
Wed 25 May 2016
SRR1780513_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780513_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322950
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCGT301.2814456E-436.7516220
CTTCGTC300.00568291929.3921621
AAGCACG300.00569587829.3784714
ATAGACA508.358814E-526.3832661
CTATACA1051.1277734E-1025.1269191
CCATAGG450.001401162124.4289511
ATGCGAC1101.9645086E-1024.03692614
ATAAAGG1202.3646862E-1123.7997884
GCGACAA1301.8189894E-1223.73613416
GTCTACT651.8169467E-523.6772921
AAAGGTG1301.8189894E-1223.6626246
CGACAAT1153.4015102E-1022.9989917
CTTTACA2350.022.4538441
TTTACAC2450.022.4173832
CAATGGA1306.548362E-1122.05097220
TCAGTGA500.002544270622.04754418
TGCGACA1205.802576E-1022.04069515
CTGACGT500.002565006522.01675812
GTCCGTA500.002565006522.01675813
TATAATC500.00256731922.01334240
Produced by FastQC (version 0.11.5)