FastQCFastQC Report
Wed 25 May 2016
SRR1780513_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780513_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322950
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT247287.6569128348041495No Hit
ATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTT207786.433813283790061Illumina PCR Primer Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC71422.211487846415854No Hit
CTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC36931.1435206688341848No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG28950.8964235949837436No Hit
TATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCT19140.5926614026939155No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC14280.44217371110078957No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC12250.379315683542344No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG10200.3158383650719926No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC8520.26381792847189967No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG6590.20405635547298343No Hit
CTTAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC5800.17959436445270166No Hit
TTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC5130.15884811890385508No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG4200.13005109150023222No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG3240.10032512772875059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT22100.042.2106744
TTTACAC8300.039.2373352
CTTATAA750.038.171081
CTTTACA8650.037.9334641
GGATCGT301.2985663E-436.6688643
CTATACA4350.036.4498331
GCCGTCT25650.036.36864543
GATCGTT250.002349558335.2021144
AGCGCAC250.00235134435.1966634
TCTTTAT2150.034.8251341
CTTTATA2150.034.7981642
TTACACA9650.033.9762043
CGTGCGA401.8171462E-532.99686410
CCGTGCG401.8171462E-532.9968649
AGGGATC353.211555E-431.4304541
CTTATAC37400.029.9119341
GCTTCTA300.00571117329.3623681
ATACCAC300.00573284129.3396324
TCGTTCA300.00573718229.33508941
ATGATTC300.00573718229.33508940
Produced by FastQC (version 0.11.5)