FastQCFastQC Report
Wed 25 May 2016
SRR1780512_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780512_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60873
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACAAG150.002158319870.456571
TGTGGCG150.002215589969.9934310
GTGACCA301.0087206E-769.993432
AGGTCCC150.002215589969.9934310
TGTGACC407.0427996E-752.8424341
TCGCCGC200.006923167552.4950749
TTGGAAC200.006923167552.4950729
GCGCCTC200.006923167552.495079
TCGCTAC200.006923167552.4950715
GAATTCA200.006923167552.4950757
TACAGGT200.006923167552.495077
CACTTGC200.006923167552.4950726
ATACAGG200.006923167552.495076
TGACCAG352.0651796E-549.9953043
TTGAATG350.001246997239.99624616
CATCGGC400.002402639234.99671639
TTCATAC400.002402639234.9967163
ATTGTAA450.00427874431.10818927
TGACCCA450.00427874431.1081893
TGAGGGG450.00427874431.10818930
Produced by FastQC (version 0.11.5)