FastQCFastQC Report
Wed 25 May 2016
SRR1780512_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780512_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60873
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT36636.017446158395347No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT34715.702035385146123Illumina PCR Primer Index 1 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC10931.7955415372989665No Hit
CTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC5840.9593744352997223No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG3660.6012517865063328No Hit
TATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT3400.5585399109621672Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGT1970.3236245954692557No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC1930.3170535376932302No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC1730.2841982488131027No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG1170.19220343994874575No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTCTT1150.18891791106073302No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGT1100.18070408884070113No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG1090.17906132439669475No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC1030.1692047377326565No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGTCTT900.14784879996057365No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC870.14292050662855452No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGT820.13470668440852268No Hit
TTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC760.12485009774448443No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG760.12485009774448443No Hit
CTTATCACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC740.12156456885647167No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGTCTT690.1133507466364398No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG630.10349415997240156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA900.066.111111
TTTACAC2000.061.2500042
CTTTACA2100.056.6666641
GGACTAG252.3730239E-456.0000041
CTTACTG252.3730239E-456.00000434
TTCAGAC200.00692059752.528
ATGGCGG200.00692059752.512
AGGGTAG200.00692059752.527
CTTATAA608.185452E-1152.51
CTTGCCC200.00692059752.547
GCGGGCA200.00692059752.515
CAGGATA200.00692059752.520
GACGGTT200.00692059752.532
GGCGGGC200.00692059752.514
ATAACAC608.185452E-1152.54
ACGGTTT200.00692059752.533
CAGACGG200.00692059752.530
AACACGA200.00692059752.570
TTACACA2450.050.03
TTATAAC651.8007995E-1048.4615362
Produced by FastQC (version 0.11.5)