##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780512_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 60873 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.983013815648974 34.0 34.0 34.0 32.0 34.0 2 33.34256566950865 34.0 34.0 34.0 33.0 34.0 3 33.510636899774944 34.0 34.0 34.0 34.0 34.0 4 33.52938084208105 34.0 34.0 34.0 34.0 34.0 5 33.529972237280894 34.0 34.0 34.0 34.0 34.0 6 37.292149228722096 38.0 38.0 38.0 36.0 38.0 7 36.90805447406896 38.0 37.0 38.0 35.0 38.0 8 36.949353572191285 38.0 37.0 38.0 35.0 38.0 9 36.7060437303895 38.0 37.0 38.0 34.0 38.0 10-11 36.41612866131125 38.0 37.0 38.0 34.0 38.0 12-13 36.27240320010513 38.0 37.0 38.0 34.0 38.0 14-15 36.42318433459826 38.0 37.0 38.0 34.0 38.0 16-17 36.496303780000986 38.0 37.0 38.0 34.0 38.0 18-19 36.56226898625006 38.0 37.0 38.0 34.0 38.0 20-21 36.33283229017791 38.0 37.0 38.0 34.0 38.0 22-23 36.34626188950766 38.0 37.0 38.0 33.5 38.0 24-25 35.54119231843346 38.0 36.0 38.0 31.0 38.0 26-27 35.78832158756756 38.0 36.0 38.0 31.5 38.0 28-29 35.698248813102694 38.0 36.0 38.0 31.0 38.0 30-31 35.44031015392703 38.0 36.0 38.0 29.0 38.0 32-33 35.997108734578546 38.0 37.0 38.0 33.0 38.0 34-35 35.730069160383096 38.0 37.0 38.0 31.5 38.0 36-37 35.631256879076105 38.0 36.5 38.0 31.0 38.0 38-39 34.93092996895175 37.5 35.5 38.0 26.5 38.0 40-41 35.01176219341909 37.0 35.5 38.0 29.0 38.0 42-43 35.69037175759368 37.5 36.5 38.0 32.5 38.0 44-45 35.87443529972238 38.0 36.5 38.0 32.0 38.0 46-47 36.102787771261475 38.0 37.0 38.0 34.0 38.0 48-49 35.84506267146354 38.0 36.5 38.0 32.0 38.0 50-51 35.93064248517405 38.0 37.0 38.0 32.0 38.0 52-53 35.79606722192105 38.0 37.0 38.0 31.5 38.0 54-55 36.02631708639298 38.0 37.0 38.0 33.5 38.0 56-57 35.993708212179456 38.0 37.0 38.0 33.0 38.0 58-59 35.989946281602684 38.0 37.0 38.0 33.0 38.0 60-61 35.74017216171373 38.0 36.5 38.0 32.5 38.0 62-63 36.14212376587321 38.0 37.0 38.0 33.5 38.0 64-65 36.11483744845827 38.0 37.0 38.0 33.0 38.0 66-67 35.13952819805168 38.0 36.0 38.0 29.0 38.0 68-69 33.59544461419677 37.0 34.0 38.0 22.0 38.0 70-71 32.575296108291035 37.0 32.0 38.0 16.0 38.0 72-73 31.545644210076716 37.0 26.0 38.0 11.0 38.0 74-75 31.868003876924085 37.0 27.5 38.0 11.0 38.0 76 31.527655939414846 37.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35805842772365537 1101 2 0.38262228808136456 1101 3 0.2173635846001858 1101 4 0.38429654318891693 1101 5 0.4202831747444904 1101 6 -0.9853030148684496 1101 7 0.22583609484266987 1101 8 0.3380052320944671 1101 9 0.37640079566823204 1101 10-11 0.2052626354215903 1101 12-13 0.5216844248140404 1101 14-15 0.5361580410764191 1101 16-17 -0.06327562582976753 1101 18-19 -0.7932081076105888 1101 20-21 -0.17694073379314545 1101 22-23 0.6569192076513062 1101 24-25 -1.3820081376896027 1101 26-27 -0.6068890286020405 1101 28-29 0.5032804713688392 1101 30-31 1.0946701741816298 1101 32-33 0.47069486748230105 1101 34-35 0.06784174934981735 1101 36-37 0.1312029124287193 1101 38-39 0.47544884950325184 1101 40-41 1.6272053587356154 1101 42-43 0.48527264241769785 1101 44-45 0.6932877762883436 1101 46-47 0.7305613020768575 1101 48-49 -0.8119864918325703 1101 50-51 -1.3764680184811482 1101 52-53 -1.7433669741329751 1101 54-55 -0.9576255174360568 1101 56-57 -1.5132873103948867 1101 58-59 -1.255179912095926 1101 60-61 -1.5884211301639724 1101 62-63 -0.9313303772898038 1101 64-65 -1.200071902475834 1101 66-67 -2.52361638467535 1101 68-69 -0.9661339490510557 1101 70-71 -1.2668492870626409 1101 72-73 -1.1926318669118317 1101 74-75 -0.8027695253114793 1101 76 -1.0231950072193818 1102 1 -0.5434639104432151 1102 2 0.06338300543240649 1102 3 0.19604181561613387 1102 4 0.42409416707652525 1102 5 0.14219671316433136 1102 6 0.44077097889763905 1102 7 0.3480297073466261 1102 8 0.6572445147434252 1102 9 0.632216850237505 1102 10-11 0.3418665112855237 1102 12-13 0.5466878128069936 1102 14-15 0.4361896623439847 1102 16-17 0.21596275335440396 1102 18-19 -0.7194176210947987 1102 20-21 0.53855820463572 1102 22-23 0.3144157067252564 1102 24-25 -5.604847727516585 1102 26-27 -1.3799612606118075 1102 28-29 -0.32000181620400525 1102 30-31 1.6006782150182417 1102 32-33 0.04611159061551717 1102 34-35 0.4947740346639975 1102 36-37 -2.8600108925132375 1102 38-39 -0.7852910881576776 1102 40-41 0.6187353763531789 1102 42-43 1.0080851283010546 1102 44-45 1.352613791556891 1102 46-47 1.4404361722037962 1102 48-49 -3.4563827514883485 1102 50-51 -0.45276561978214147 1102 52-53 -0.18814268899949127 1102 54-55 0.6382095098937555 1102 56-57 0.7755729687757338 1102 58-59 0.07736288954838244 1102 60-61 -6.458051161333515 1102 62-63 -2.587358746084128 1102 64-65 0.21985853073552875 1102 66-67 1.4996930068411132 1102 68-69 -0.9220448674230113 1102 70-71 -1.698344069553496 1102 72-73 -5.55833182625592 1102 74-75 -2.900208202639032 1102 76 -1.017638698776505 1103 1 0.7077038177945738 1103 2 0.5153646048662353 1103 3 0.03036594866638609 1103 4 0.3623107275860775 1103 5 0.14711532604472666 1103 6 0.09554804896133362 1103 7 0.8068053619821498 1103 8 0.40526291530959213 1103 9 0.0046513866847845975 1103 10-11 0.25679927844758765 1103 12-13 -0.7739420527273353 1103 14-15 -0.4239483419023031 1103 16-17 -0.036443474529534114 1103 18-19 0.3537777079922435 1103 20-21 -0.7019407337931511 1103 22-23 0.3072147159255394 1103 24-25 -3.383899390644686 1103 26-27 -0.9072436385311278 1103 28-29 0.031767469004769566 1103 30-31 0.9883462971130754 1103 32-33 0.964193685449203 1103 34-35 0.36405924343965523 1103 36-37 -1.7499436596753029 1103 38-39 -0.4441728999057375 1103 40-41 -0.4816007878364914 1103 42-43 -0.12004650651847726 1103 44-45 1.091396523333259 1103 46-47 0.6955140207529737 1103 48-49 -2.433735900816025 1103 50-51 -0.6487493423582151 1103 52-53 0.2950491015171437 1103 54-55 -0.769327645095629 1103 56-57 0.24268195674459747 1103 58-59 -0.46363144873895124 1103 60-61 -4.298054699903933 1103 62-63 -1.5262476349730179 1103 64-65 0.07049547341068774 1103 66-67 -0.6060040915311404 1103 68-69 -0.005732057798098822 1103 70-71 0.3268859375236417 1103 72-73 -3.6178682735312186 1103 74-75 -1.8824976576991865 1103 76 -2.545062619512528 1104 1 0.23290706982709253 1104 2 0.35831853440553374 1104 3 0.36180226302952434 1104 4 -0.0011039065602602705 1104 5 -0.5340499856083909 1104 6 -0.0300617071362268 1104 7 0.5940682346108801 1104 8 0.508650449184934 1104 9 0.918608026251178 1104 10-11 -0.21955573510254567 1104 12-13 0.4812069987631844 1104 14-15 0.4988158857399654 1104 16-17 0.16836682357345012 1104 18-19 0.568818358398751 1104 20-21 -0.09055861997201475 1104 22-23 0.17720116579003076 1104 24-25 0.6554637529867477 1104 26-27 1.3065774996802588 1104 28-29 1.3257376587066716 1104 30-31 0.7679533431835281 1104 32-33 0.3242885363977095 1104 34-35 -0.3206291034438138 1104 36-37 0.9422650124114185 1104 38-39 1.4382796746200057 1104 40-41 2.1530875590469805 1104 42-43 0.7350347942945348 1104 44-45 0.8374669840378601 1104 46-47 0.7594706603193728 1104 48-49 0.32371667370972546 1104 50-51 0.489191037045579 1104 52-53 0.8678810798369199 1104 54-55 0.47742032238404875 1104 56-57 0.4697822277473023 1104 58-59 -0.3175609880343444 1104 60-61 0.5322976036191065 1104 62-63 0.33032418074514425 1104 64-65 0.7558613270692263 1104 66-67 0.08620458055557378 1104 68-69 1.8353926034485113 1104 70-71 0.9350837695019649 1104 72-73 0.2700043551950735 1104 74-75 -0.4311426441490518 1104 76 -0.21714934037973777 1105 1 0.6888956241260473 1105 2 0.29652537457449313 1105 3 0.1785347878966732 1105 4 -0.2695328478329131 1105 5 -0.45229497811418184 1105 6 0.10336927801161266 1105 7 0.5760480683756981 1105 8 -0.6764565136165359 1105 9 0.6348500210733619 1105 10-11 -0.10794311757972253 1105 12-13 -0.6737868696112557 1105 14-15 0.01157307336771396 1105 16-17 -0.011272773101843825 1105 18-19 0.6073624379736273 1105 20-21 0.11169986211001515 1105 22-23 0.2241762305127466 1105 24-25 0.5273979402057165 1105 26-27 0.8012293596066797 1105 28-29 1.0250325217670309 1105 30-31 0.9207330134383369 1105 32-33 0.8969993961878728 1105 34-35 -0.627312575306469 1105 36-37 0.39147471400563916 1105 38-39 0.04961977545119112 1105 40-41 0.7998548297923094 1105 42-43 -0.0698602867791891 1105 44-45 0.552414524574715 1105 46-47 1.1579752249739599 1105 48-49 -0.1885465774144066 1105 50-51 0.5393170760154646 1105 52-53 0.4069361282086419 1105 54-55 0.5447319452209456 1105 56-57 0.7829209387433522 1105 58-59 0.3519954910500047 1105 60-61 0.6398565353536725 1105 62-63 -0.6236715952461438 1105 64-65 0.36183625553359633 1105 66-67 0.48583327656321273 1105 68-69 -0.329599221050735 1105 70-71 0.28961095304195794 1105 72-73 0.02916772895171249 1105 74-75 -0.5176155968674045 1105 76 -0.612877641238164 1106 1 -0.6968713456041158 1106 2 0.3281097029054507 1106 3 0.25977771337226585 1106 4 0.1420492896775798 1106 5 0.010187221469564633 1106 6 0.2576395522293069 1106 7 -0.7699920236387641 1106 8 0.046112588512208674 1106 9 -0.016590443380579245 1106 10-11 0.4541848993626232 1106 12-13 1.2525285355079632 1106 14-15 -0.19273919157550523 1106 16-17 0.5495042378887689 1106 18-19 0.632045681848453 1106 20-21 0.44070632503037643 1106 22-23 0.3737179838993896 1106 24-25 -0.3263830507754051 1106 26-27 0.8643249889198543 1106 28-29 0.8092184493969228 1106 30-31 0.7741306108385615 1106 32-33 0.2795531625733858 1106 34-35 0.9732095702370387 1106 36-37 1.4884550331351534 1106 38-39 0.4381800412707406 1106 40-41 1.245686793862859 1106 42-43 1.1580580686449196 1106 44-45 0.9776710331371774 1106 46-47 0.9574421253281358 1106 48-49 1.0335843606218873 1106 50-51 0.4553356249620464 1106 52-53 0.8228268792949223 1106 54-55 0.33099915228999066 1106 56-57 0.3634335907315247 1106 58-59 0.6404535185813103 1106 60-61 0.8877296138215556 1106 62-63 0.9910726264648915 1106 64-65 -0.7951908010991176 1106 66-67 0.5825579999721242 1106 68-69 2.097209118672488 1106 70-71 1.1363630617066534 1106 72-73 1.7424258441158393 1106 74-75 0.904920642954405 1106 76 1.3389243085920448 1107 1 0.5200847701755293 1107 2 -0.5695560300544003 1107 3 0.056715155015588437 1107 4 0.38231072758608065 1107 5 0.10441691334631287 1107 6 0.2269766203899053 1107 7 0.6666466318234185 1107 8 -0.3098164497697695 1107 9 -0.5437613117279128 1107 10-11 0.42854531019362696 1107 12-13 0.26970874092346264 1107 14-15 0.2977183247643609 1107 16-17 0.7008581127720603 1107 18-19 0.5592538984684339 1107 20-21 0.9221068852544647 1107 22-23 0.19110360481442257 1107 24-25 0.4767355299902363 1107 26-27 -0.17248173376921727 1107 28-29 0.628751595988895 1107 30-31 0.4196955034622718 1107 32-33 0.774828606084121 1107 34-35 0.2996147989952149 1107 36-37 1.1733103085786638 1107 38-39 0.38693821120537564 1107 40-41 0.30070079946510475 1107 42-43 0.5251915887196219 1107 44-45 0.7177457296824628 1107 46-47 1.0206727509117073 1107 48-49 0.5230894960093693 1107 50-51 0.8559331973243189 1107 52-53 0.6786998951679379 1107 54-55 0.6854342596662732 1107 56-57 1.1256184646811036 1107 58-59 0.48486061475311715 1107 60-61 0.9672627604135258 1107 62-63 0.4983555396301611 1107 64-65 0.6090669019821178 1107 66-67 0.04939273386568743 1107 68-69 1.2893473072812682 1107 70-71 0.23053673117443196 1107 72-73 0.9598301391671917 1107 74-75 -0.4306722608737914 1107 76 0.6681119836620759 1108 1 -0.18144346902712982 1108 2 0.33293566429776433 1108 3 -0.13139115727676653 1108 4 0.22975258805119836 1108 5 -0.12239371788292175 1108 6 -0.11840543941075765 1108 7 -0.09694140804369056 1108 8 -0.519284888307979 1108 9 -0.9869506804986798 1108 10-11 0.08638584175508868 1108 12-13 0.8971173787972191 1108 14-15 0.5101601852294735 1108 16-17 -0.5623478672943847 1108 18-19 -0.4797494237907003 1108 20-21 0.604067018144832 1108 22-23 -0.5963382556506858 1108 24-25 0.5985295499237893 1108 26-27 -0.7355382121745322 1108 28-29 -1.26558395218386 1108 30-31 -0.06585266929519662 1108 32-33 0.6838319283432597 1108 34-35 0.4794985199254427 1108 36-37 0.7239581491102243 1108 38-39 0.6233335600425818 1108 40-41 0.23958784265446553 1108 42-43 -0.5946589096192554 1108 44-45 0.6854533708784771 1108 46-47 0.5283305582206808 1108 48-49 0.45935195448113575 1108 50-51 0.536198978055225 1108 52-53 -0.40133332742342986 1108 54-55 -0.42785477687857565 1108 56-57 -0.14904930940527805 1108 58-59 0.04205330578301414 1108 60-61 0.7111830261942558 1108 62-63 0.5463621841484283 1108 64-65 0.42049547341068916 1108 66-67 0.24871167074275746 1108 68-69 0.9141645830287715 1108 70-71 -1.51833370071925 1108 72-73 -1.2454522528593834 1108 74-75 -1.1789446861229607 1108 76 -0.7143132655073607 1109 1 -1.2384786775499315 1109 2 0.2797655980381819 1109 3 0.25674707839947075 1109 4 0.13828838121736453 1109 5 0.20133630680201975 1109 6 -0.2318262527146402 1109 7 -0.6138960657025194 1109 8 0.3682362238136321 1109 9 0.46725225571023543 1109 10-11 0.5009954299063111 1109 12-13 0.45191145441108915 1109 14-15 0.7573831292336379 1109 16-17 0.8825819755015019 1109 18-19 0.6604350636719474 1109 20-21 0.8575099179759391 1109 22-23 0.204623157887049 1109 24-25 0.22851525864147249 1109 26-27 0.6224584098860078 1109 28-29 -0.32133619331676755 1109 30-31 1.2838615050584465 1109 32-33 0.8467200665789321 1109 34-35 0.8922404973192357 1109 36-37 1.3886814179162599 1109 38-39 1.1809046916523016 1109 40-41 0.7663352767196869 1109 42-43 0.9999721154554493 1109 44-45 0.8987832396585205 1109 46-47 0.9764109791639015 1109 48-49 1.1466489533118462 1109 50-51 0.3884790871886423 1109 52-53 -0.2494884527969532 1109 54-55 -0.06420660226508801 1109 56-57 0.422585743212629 1109 58-59 0.3128893457986024 1109 60-61 1.1538230158006044 1109 62-63 0.4685071757035857 1109 64-65 0.8283167024609739 1109 66-67 0.4243807625967264 1109 68-69 0.1843672593961898 1109 70-71 0.9236891653324548 1109 72-73 0.6660392373315993 1109 74-75 -0.15169380915790853 1109 76 0.37036258222552476 1110 1 -1.3759219131995764 1110 2 -0.6282026465957529 1110 3 -0.5035521799885885 1110 4 -0.1283325472677248 1110 5 0.11992234358858411 1110 6 -0.05298996273457135 1110 7 0.07142524502308589 1110 8 0.6061401082920526 1110 9 0.813130918848529 1110 10-11 -0.5098673882190781 1110 12-13 0.5555901110153556 1110 14-15 0.8662563364602676 1110 16-17 0.7971822564646232 1110 18-19 -0.09344451422997935 1110 20-21 0.9838779796571373 1110 22-23 -0.4487794361212494 1110 24-25 -1.547057285381527 1110 26-27 0.7790136714103966 1110 28-29 0.21100723508664032 1110 30-31 -0.23636130522611154 1110 32-33 0.5299831591334154 1110 34-35 1.0038253253110057 1110 36-37 1.5355826561141726 1110 38-39 0.6143066322580069 1110 40-41 -1.587302542222453 1110 42-43 0.15977805488503094 1110 44-45 0.8820397981870585 1110 46-47 0.6839643131506321 1110 48-49 1.3352699471371992 1110 50-51 -0.3009423248143577 1110 52-53 0.521657288651646 1110 54-55 -0.1234212123470968 1110 56-57 0.6451673368615545 1110 58-59 -0.8263507469845592 1110 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-1.0428337425947873 2116 48-49 0.29750508042496193 2116 50-51 -1.2054953741042453 2116 52-53 -0.07688452041648475 2116 54-55 -0.8723579481259378 2116 56-57 -0.06399192492929018 2116 58-59 -2.227087437194939 2116 60-61 -1.541308668157896 2116 62-63 -0.03865744738282473 2116 64-65 -2.4431408902256706 2116 66-67 -0.7656722011992514 2116 68-69 -0.9880552901213306 2116 70-71 1.2815756922134014 2116 72-73 0.202297671634728 2116 74-75 0.2646524144508824 2116 76 -0.802277626727534 2117 1 0.7033013020713028 2117 2 0.5680899717425447 2117 3 0.3823575629221452 2117 4 0.16671324330935278 2117 5 -0.0049811100139720565 2117 6 0.1986927030493888 2117 7 0.6658200370345639 2117 8 0.007883460382970497 2117 9 -0.593356999059452 2117 10-11 -0.6524250402106873 2117 12-13 -1.324361339939074 2117 14-15 0.020234048034801333 2117 16-17 -1.0923134954938902 2117 18-19 -0.8114738643348005 2117 20-21 0.09664417186722574 2117 22-23 0.5736193280848667 2117 24-25 -1.6004612355084191 2117 26-27 -1.7930926951349022 2117 28-29 -1.1180228873893583 2117 30-31 -0.23591995026638557 2117 32-33 -2.328783253754146 2117 34-35 0.46741354113357403 2117 36-37 -1.1424489007654515 2117 38-39 1.122913053972674 2117 40-41 0.1598562352243107 2117 42-43 0.49095978278969454 2117 44-45 -0.4182470825367659 2117 46-47 -0.6762365033560371 2117 48-49 -0.6276562362457909 2117 50-51 -0.2686130740144037 2117 52-53 0.6917996256261603 2117 54-55 -0.47341569541009676 2117 56-57 0.7034261915454252 2117 58-59 -1.8759480105838122 2117 60-61 0.14916752231828667 2117 62-63 -0.4772958530023672 2117 64-65 -1.1361083001742216 2117 66-67 -0.6486665653256907 2117 68-69 0.31869142228575953 2117 70-71 0.6500515559722828 2117 72-73 1.5438193574960302 2117 74-75 1.6023555917767034 2117 76 1.7259017410744768 2118 1 0.33203919611768384 2118 2 0.3194886675792574 2118 3 -0.2709973778503212 2118 4 0.1371573533529471 2118 5 0.374075107912482 2118 6 0.1188609323969203 2118 7 0.7565326966788106 2118 8 0.4502186071978045 2118 9 -1.3714563161100344 2118 10-11 -0.7794652068966457 2118 12-13 -0.8550940636339419 2118 14-15 0.7036078953165088 2118 16-17 -2.4959867601464367 2118 18-19 -0.617643559901417 2118 20-21 -0.3190675231592053 2118 22-23 0.8030054453052244 2118 24-25 0.7116697981760396 2118 26-27 -2.1165483420076043 2118 28-29 -0.22893464415133025 2118 30-31 -0.5285078269496424 2118 32-33 -0.18594264720246656 2118 34-35 -0.44418888198638484 2118 36-37 -4.036225186601001 2118 38-39 -0.2563746722302085 2118 40-41 -0.2179845642509406 2118 42-43 0.5131845773261006 2118 44-45 -1.9062858215883551 2118 46-47 -0.17093110535472533 2118 48-49 0.6037555783932547 2118 50-51 -0.7635584766458834 2118 52-53 -1.7695384922115522 2118 54-55 -1.1884482994923644 2118 56-57 0.6534011114821467 2118 58-59 -0.12036287341516072 2118 60-61 -0.0921412711377414 2118 62-63 -0.7867452491516147 2118 64-65 0.4573052893616065 2118 66-67 0.175965915285488 2118 68-69 -1.5795221055145277 2118 70-71 -0.5775789568185523 2118 72-73 0.6301719446010274 2118 74-75 -0.48779758953286034 2118 76 0.3668324043456863 2119 1 0.8260044713893464 2119 2 -0.6326746108200396 2119 3 -0.06701967224864802 2119 4 -0.5050095338518261 2119 5 -0.31661016415134924 2119 6 -0.39595521901252084 2119 7 -0.9464626118740611 2119 8 0.06572043164946706 2119 9 0.14680824942988124 2119 10-11 -0.09810268233673014 2119 12-13 -0.34877865403452546 2119 14-15 -0.11757579288269682 2119 16-17 -0.41128007583672144 2119 18-19 -0.5117451678247491 2119 20-21 -0.5985093612441261 2119 22-23 0.1384238662523316 2119 24-25 1.261852243342247 2119 26-27 0.7499459039525362 2119 28-29 0.29614655397209333 2119 30-31 0.7414508722764666 2119 32-33 0.7632132932923454 2119 34-35 0.4993533610867118 2119 36-37 0.1616576475142395 2119 38-39 -1.1010356450037762 2119 40-41 -1.7118295460064203 2119 42-43 0.2855090490370742 2119 44-45 0.22930502006528286 2119 46-47 0.019533628596114738 2119 48-49 -0.250729364868306 2119 50-51 0.7331134027398178 2119 52-53 0.22151968975244074 2119 54-55 0.28197954444685536 2119 56-57 -1.2411415726671677 2119 58-59 -0.0033373310918918264 2119 60-61 0.30603026741632533 2119 62-63 -0.057946981378243834 2119 64-65 -0.10891629129519487 2119 66-67 1.1054338169655864 2119 68-69 0.5057058506986323 2119 70-71 -0.40938857228027814 2119 72-73 0.4385042754883912 2119 74-75 -0.40226889952925404 2119 76 0.03173476610838577 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 1.0 22 6.0 23 26.0 24 35.0 25 100.0 26 188.0 27 357.0 28 586.0 29 907.0 30 1513.0 31 2237.0 32 3290.0 33 4903.0 34 7013.0 35 8674.0 36 11230.0 37 19805.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.732837218471243 24.21927619798597 11.535491925812757 33.51239465773003 2 15.060864422650436 17.321308297603206 47.61388464508074 20.003942634665613 3 14.042350467366486 27.77093292592775 37.627519589966 20.55919701673977 4 10.024148637326894 24.813956926716276 35.95682815041151 29.205066285545318 5 9.252049348643897 40.821053669114384 38.35197870977281 11.57491827246891 6 20.01872751466167 40.90812018464673 16.225584413450957 22.84756788724065 7 19.98915775466956 36.80613736796281 19.410904670379313 23.79380020698832 8 17.181673319862664 37.33017922560084 24.39176646460664 21.096380989929852 9 17.66464606640054 19.49304289257963 18.10326417295024 44.739046868069586 10-11 18.56405959949403 25.497346935422932 31.584610582688548 24.353982882394494 12-13 22.43851954068306 18.75297751055476 28.014883445862697 30.793619502899478 14-15 23.173656629375913 23.424178207086886 19.96944458134148 33.43272058219572 16-17 24.73921114451399 27.39391848602829 19.567788674781923 28.2990816946758 18-19 22.37855863847683 26.404974290736448 20.660227030046162 30.556240040740555 20-21 20.734151430026447 23.8397976114205 20.36945772345703 35.05659323509602 22-23 23.225403709362112 30.345966191907742 20.610122714503966 25.818507384226173 24-25 24.60286169566146 32.298391733609314 20.38424260345309 22.71450396727613 26-27 25.536773282079082 28.993971054490498 25.309250406584198 20.16000525684622 28-29 24.027072758037225 22.38430831403085 31.586253347132555 22.002365580799367 30-31 28.05841670362887 24.660358451201684 24.859132948926455 22.422091896243 32-33 18.355428515105217 27.78818195258982 26.122418806367353 27.733970725937606 34-35 17.060930133228197 32.40681418691374 25.536773282079082 24.995482397778982 36-37 16.781660177747113 32.83803985346541 27.116291294991214 23.264008673796262 38-39 20.298818852364757 23.75190971366616 28.30483137022982 27.64444006373926 40-41 18.670839288354443 24.153565620225717 31.58214643602254 25.593448655397307 42-43 25.366747162124426 22.558441345095524 33.058170288962266 19.016641203817784 44-45 18.9435381860595 25.400423833226554 29.527048116570565 26.12898986414338 46-47 25.52280978430503 20.51319961230759 27.444022801570483 26.519967801816897 48-49 22.526407438437403 19.85527245248304 28.865014045635995 28.753306063443564 50-51 16.95661459103379 20.122221674634076 34.00193846204393 28.91922527228821 52-53 18.91561119051139 20.41463374566721 38.63946248747392 22.030292576347478 54-55 17.047788017676147 20.42202618566524 34.75925287073087 27.77093292592775 56-57 23.85622525586056 20.14275623018415 30.468352142986216 25.532666370969064 58-59 17.874098533011352 28.74755638788954 33.13291607116455 20.24542900793455 60-61 20.072938741313884 37.35810622114895 23.56956286038145 18.99939217715572 62-63 14.936014324905953 44.39735186371626 21.252443612110458 19.414190199267328 64-65 14.826770489379529 45.518538596750616 20.250357301266572 19.40433361260329 66-67 14.707670067189065 43.983375223826656 20.516485141195602 20.792469567788675 68-69 14.608282818326678 42.1130879043254 20.93292592775122 22.3457033495967 70-71 15.395166987005734 39.42881080281899 21.473395429829317 23.702626780345966 72-73 15.854319649105515 36.07346442593597 21.838910518620736 26.233305406337788 74-75 16.137696515696614 35.47796231498365 22.084503802999688 26.299837366320045 76 16.337292395643388 35.78105235490283 21.76827164752846 26.11338360192532 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.5 15 1.0 16 3.0 17 5.0 18 4.0 19 6.0 20 12.0 21 15.0 22 21.5 23 31.5 24 50.5 25 66.0 26 88.5 27 155.0 28 230.5 29 262.0 30 315.0 31 421.0 32 603.5 33 733.0 34 822.5 35 1063.0 36 1304.0 37 1394.0 38 1721.0 39 2463.5 40 4052.5 41 6076.0 42 6926.0 43 6850.0 44 6304.5 45 5366.0 46 4897.0 47 4566.5 48 3808.5 49 3137.0 50 2893.0 51 2651.5 52 2145.5 53 1736.5 54 1592.0 55 1481.5 56 1178.0 57 802.0 58 619.0 59 529.0 60 366.5 61 238.0 62 182.0 63 138.0 64 65.0 65 31.5 66 18.5 67 10.0 68 5.0 69 1.0 70 1.5 71 1.0 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.5 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 60873.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.31508222036042 #Duplication Level Percentage of deduplicated Percentage of total 1 62.44651807179822 25.175365104397677 2 17.020496312293712 13.723654165229249 3 8.210749358216862 9.93051106401853 4 4.217432052483598 6.801044798186388 5 2.4530377735218614 4.944720976459186 6 1.5199054643250072 3.6765068256862645 7 1.0350026486288253 2.9208351814433327 8 0.7945886475693738 2.562712532649943 9 0.4278554256142782 1.5524123995860233 >10 1.7847683468481317 10.22949419282769 >50 0.032598508618230715 1.0135856619519328 >100 0.040748135772788394 2.9947595814236196 >500 0.004074813577278839 0.9593744352997223 >1k 0.012224440731836518 13.515023080840438 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT 3663 6.017446158395347 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT 3471 5.702035385146123 Illumina PCR Primer Index 1 (95% over 22bp) CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 1093 1.7955415372989665 No Hit CTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 584 0.9593744352997223 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 366 0.6012517865063328 No Hit TATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT 340 0.5585399109621672 Illumina PCR Primer Index 1 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGT 197 0.3236245954692557 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 193 0.3170535376932302 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 173 0.2841982488131027 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 117 0.19220343994874575 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTCTT 115 0.18891791106073302 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGT 110 0.18070408884070113 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 109 0.17906132439669475 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 103 0.1692047377326565 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGTCTT 90 0.14784879996057365 No Hit CTTAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 87 0.14292050662855452 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGT 82 0.13470668440852268 No Hit TTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 76 0.12485009774448443 No Hit CTTATTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 76 0.12485009774448443 No Hit CTTATCACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 74 0.12156456885647167 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGTCTT 69 0.1133507466364398 No Hit CTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 63 0.10349415997240156 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0016427644440063739 0.0 2 0.0 0.0 0.0 0.0032855288880127478 0.0 3 0.0 0.0 0.0 0.0032855288880127478 0.0 4 0.0 0.0 0.0 0.0032855288880127478 0.0 5 0.0 0.0 0.0 0.0065710577760254955 0.0 6 0.0 0.0 0.0 0.0065710577760254955 0.0 7 0.0 0.0 0.0 0.0065710577760254955 0.0 8 0.0 0.0 0.0 0.00821382222003187 0.0 9 0.0 0.0 0.0 0.013142115552050991 0.0 10 0.0 0.0 0.0 0.014784879996057366 0.0 11 0.0 0.0 0.0 0.01642764444006374 0.0 12 0.0 0.0 0.0 0.01642764444006374 0.0 13 0.0 0.0 0.0 0.018070408884070112 0.0 14 0.0 0.0 0.0 0.019713173328076487 0.0 15 0.0 0.0 0.0 0.019713173328076487 0.0 16 0.0 0.0 0.0 0.019713173328076487 0.0 17 0.0 0.0 0.0 0.027926995548108357 0.0 18 0.0 0.0 0.0 0.03449805332413385 0.0 19 0.0 0.0 0.0 0.03942634665615297 0.0 20 0.0 0.0 0.0 0.04271187554416572 0.0 21 0.0 0.0 0.0 0.044354639988172094 0.0 22 0.0 0.0 0.0 0.049282933320191215 0.0 23 0.0 0.0 0.0 0.052568462208203964 0.0 24 0.0 0.0 0.0 0.05913951998422946 0.0 25 0.0 0.0 0.0 0.062425048872242206 0.0 26 0.0 0.0 0.0 0.06571057776025496 0.0 27 0.0 0.0 0.0 0.08870927997634419 0.0 28 0.0 0.0 0.0 0.10185139552839519 0.0 29 0.0 0.0 0.0 0.12813562663249717 0.0 30 0.0 0.0 0.0 0.15441985773659914 0.0 31 0.0 0.0 0.0 0.18070408884070113 0.0 32 0.0 0.0 0.0 0.21848767105284772 0.0 33 0.0 0.0 0.0 0.2644850754850262 0.0 34 0.0 0.0 0.0 0.3367667110213067 0.0 35 0.0 0.0 0.0 0.4156194043336126 0.0 36 0.0 0.0 0.0 0.5191135643060142 0.0 37 0.0 0.0 0.0 0.7343157064708491 0.0 38 0.0 0.0 0.0 1.0217994841719646 0.0 39 0.0 0.0 0.0 1.3864931907413796 0.0 40 0.0 0.0 0.0 1.813611946183037 0.0 41 0.0 0.0 0.0 2.258801110508764 0.0 42 0.0 0.0 0.0 2.86333842590311 0.0 43 0.0 0.0 0.0 3.4251638657532895 0.0 44 0.0 0.0 0.0 3.9787754833834375 0.0 45 0.0 0.0 0.0 4.453534407701279 0.0 46 0.0 0.0 0.0 4.939792683127166 0.0 47 0.0 0.0 0.0 5.531187882969461 0.0 48 0.0 0.0 0.0 6.093013322819641 0.0 49 0.0 0.0 0.0 6.6630525848898525 0.0 50 0.0 0.0 0.0 7.182166149195867 0.0 51 0.0 0.0 0.0 7.739063295714027 0.0 52 0.0 0.0 0.0 8.322244673336291 0.0 53 0.0 0.0 0.0 8.833144415422273 0.0 54 0.0 0.0 0.0 9.337473099732229 0.0 55 0.0 0.0 0.0 9.818803081826097 0.0 56 0.0 0.0 0.0 10.286990948367913 0.0 57 0.0 0.0 0.0 10.801176219341908 0.0 58 0.0 0.0 0.0 11.31700425475991 0.0 59 0.0 0.0 0.0 11.796691472409771 0.0 60 0.0 0.0 0.0 12.256665516731555 0.0 61 0.0 0.0 0.0 12.765922494373532 0.0 62 0.0 0.0 0.0 13.216039952031279 0.0 63 0.0 0.0 0.0 13.743367338557324 0.0 64 0.0 0.0 0.0 14.264123667307345 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACA 90 0.0 66.11111 1 TTTACAC 200 0.0 61.250004 2 CTTTACA 210 0.0 56.666664 1 GGACTAG 25 2.3730239E-4 56.000004 1 CTTACTG 25 2.3730239E-4 56.000004 34 TTCAGAC 20 0.006920597 52.5 28 ATGGCGG 20 0.006920597 52.5 12 AGGGTAG 20 0.006920597 52.5 27 CTTATAA 60 8.185452E-11 52.5 1 CTTGCCC 20 0.006920597 52.5 47 GCGGGCA 20 0.006920597 52.5 15 CAGGATA 20 0.006920597 52.5 20 GACGGTT 20 0.006920597 52.5 32 GGCGGGC 20 0.006920597 52.5 14 ATAACAC 60 8.185452E-11 52.5 4 ACGGTTT 20 0.006920597 52.5 33 CAGACGG 20 0.006920597 52.5 30 AACACGA 20 0.006920597 52.5 70 TTACACA 245 0.0 50.0 3 TTATAAC 65 1.8007995E-10 48.461536 2 >>END_MODULE