FastQCFastQC Report
Wed 25 May 2016
SRR1780511_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780511_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448580
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAC454.097E-529.298483
GCACGCA300.005774663329.298489
CGTGGAT407.0053054E-427.51332935
GGTCGAT407.0190354E-427.50411610
CACGCAG407.0190354E-427.50411610
GACGAAT407.0282014E-427.49797823
CGGTGGT1350.026.04599643
TCAGTTA450.001403814124.42356741
CGTTAAA551.5742352E-424.03847318
GCCGTTA551.5742352E-424.03847316
CTTTATA651.8268252E-523.6641562
AGTCGTA602.837953E-422.03526920
GTCGATG602.8659683E-422.00329411
CGTAACA602.8702992E-421.9983823
AGTTATA500.002594107521.9763143
TGCCGCT856.091397E-620.70898210
TTGTACT856.162396E-620.681284
CTCTAAT755.4611857E-520.5341171
TCGGTGG3000.020.51579942
CGGATCC755.5083005E-520.51122344
Produced by FastQC (version 0.11.5)