FastQCFastQC Report
Wed 25 May 2016
SRR1780511_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780511_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448580
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT322507.189353069686566No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT280606.255294484818761RNA PCR Primer, Index 32 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC81501.8168442641223415No Hit
CTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC44410.9900129296892416No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG39150.8727540238084622No Hit
TATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT26920.6001159213518213No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC19990.44562842748227743No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC14960.3334968121628249No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG12390.2762049132819118No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC10920.24343483882473582No Hit
CTTATAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG8250.18391368317802845No Hit
CTTAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC7600.16942351420036558No Hit
TTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC5590.12461545320790049No Hit
CTTATTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG5180.11547550046814392No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG4950.11034820990681705No Hit
CTTATCACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC4690.10455214231575193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCT207.854576E-443.99632326
CCGTCTT31200.042.52515844
CTTATAA1650.040.01451
GCCGTCT35200.037.7552643
CGACAAT301.3004699E-436.663617
TTTACAC10000.035.6449662
CTATACA6350.035.351391
CTTTACA10150.035.1260261
TTACACA11400.031.6535343
TTGCGTT353.2141345E-431.4294538
ATTTGCG353.2141345E-431.4294536
CTTTATA2350.030.8979232
TTATACA49800.030.6629072
CTTATAC49900.030.6523881
GAATATG300.005731051329.3439661
TCTTTAT2550.029.3439641
ATTACAC901.6370905E-1129.3374214
TACCATT300.00573730229.337422
GTATACG300.00573730229.3374244
TCGCATA300.005740429329.3341540
Produced by FastQC (version 0.11.5)