FastQCFastQC Report
Wed 25 May 2016
SRR1780510_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780510_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59471
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAG200.00660715853.1165961
ATGGGTT200.00692442152.491177
AGATCTC200.00692442152.4911740
TATGGGT200.00692442152.491176
AATTACT200.00692442152.4911736
TCTGGCC200.00692442152.491178
GAAACCC351.9254609E-550.5872341
TTATATG305.8391225E-446.6588173
GTTATAT305.8391225E-446.6588172
GATCTCG350.001247220839.9932741
GAAACAC400.002267194735.4110641
GGCCTAT400.00240307134.99411411
TTACTAT501.692761E-434.994113
GGTTATA450.004038101531.4765031
AGCAAAC450.004279514331.10587917
GGTTAGG657.350334E-427.239281
TAAATAG700.001214111924.99579452
ACAACTC850.003752920320.5847725
ATAACCA850.003752920320.58477226
GATGGGA900.005224465419.44117470
Produced by FastQC (version 0.11.5)