##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780510_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 59471 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.895142170133344 34.0 23.0 34.0 12.0 34.0 2 28.933042995745826 34.0 23.0 34.0 12.0 34.0 3 29.159539943838173 34.0 27.0 34.0 11.0 34.0 4 29.421062366531586 34.0 27.0 34.0 11.0 34.0 5 29.267912091607673 34.0 27.0 34.0 11.0 34.0 6 31.72438667585882 37.0 27.0 38.0 11.0 38.0 7 30.954145718081083 36.0 27.0 38.0 11.0 38.0 8 30.510820399858755 36.0 26.0 38.0 11.0 38.0 9 31.031359822434464 36.0 26.0 38.0 12.0 38.0 10-11 31.14813942930168 37.0 26.5 38.0 11.0 38.0 12-13 31.464797968757882 37.0 27.0 38.0 11.0 38.0 14-15 31.10902792957912 37.0 26.5 38.0 11.0 38.0 16-17 30.902927477257823 37.0 26.0 38.0 11.0 38.0 18-19 30.977779085604748 37.0 26.5 38.0 11.0 38.0 20-21 31.01034958214928 37.0 26.5 38.0 11.0 38.0 22-23 30.884069546501657 37.0 26.0 38.0 11.0 38.0 24-25 31.291167123471944 37.0 27.0 38.0 11.0 38.0 26-27 31.251677288089994 37.0 26.0 38.0 11.0 38.0 28-29 31.21693766709825 37.0 26.5 38.0 11.0 38.0 30-31 31.12553177178793 37.0 26.0 38.0 11.0 38.0 32-33 31.010820399858755 37.0 26.0 38.0 11.0 38.0 34-35 30.952901414134622 37.0 26.0 38.0 11.0 38.0 36-37 30.86178977989272 37.0 26.0 38.0 11.0 38.0 38-39 31.16520657127003 37.0 26.0 38.0 11.0 38.0 40-41 30.817289098888534 37.0 26.0 38.0 11.0 38.0 42-43 31.127356190412137 37.0 26.0 38.0 11.0 38.0 44-45 30.32905954162533 36.0 25.0 38.0 11.0 38.0 46-47 30.981545627280525 37.0 26.0 38.0 11.0 38.0 48-49 30.855820483933343 37.0 26.0 38.0 11.0 38.0 50-51 30.80213045013536 36.5 26.0 38.0 11.0 38.0 52-53 30.745052210321 37.0 26.0 38.0 11.0 38.0 54-55 30.523002808091338 36.0 25.0 38.0 11.0 38.0 56-57 30.458408299843622 36.5 25.0 38.0 11.0 38.0 58-59 30.397664407862656 36.5 24.5 38.0 11.0 38.0 60-61 30.486430361016296 36.5 25.0 38.0 11.0 38.0 62-63 30.449437540986363 36.0 25.0 38.0 11.0 38.0 64-65 30.52839198937297 36.5 25.0 38.0 11.0 38.0 66-67 30.520699164298566 36.5 25.0 38.0 11.0 38.0 68-69 30.009357501975753 36.0 23.5 38.0 11.0 38.0 70-71 29.83295219518757 36.0 23.5 38.0 11.0 38.0 72-73 29.895125355215146 36.0 24.0 38.0 11.0 38.0 74-75 29.660565653848096 35.5 23.5 38.0 11.0 38.0 76 28.901918582166097 34.0 22.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.3691006895710167 1101 2 0.01745096690473602 1101 3 0.31264383689398656 1101 4 -1.4237875323840612 1101 5 -1.5872284455075665 1101 6 -1.381456628303873 1101 7 -0.8093304516896964 1101 8 -1.2747699312482936 1101 9 -0.615324180006521 1101 10-11 -0.9760549430604541 1101 12-13 -0.7716570884150435 1101 14-15 -0.31239037372893463 1101 16-17 -1.595387279458393 1101 18-19 -0.32914805101696487 1101 20-21 -1.5474685633856318 1101 22-23 -0.7295715719331355 1101 24-25 -0.841154823354703 1101 26-27 -0.6018860259817487 1101 28-29 -0.7236457739291247 1101 30-31 -0.9399006274552733 1101 32-33 -0.8292366399855133 1101 34-35 -1.1305805675700995 1101 36-37 -0.2121553977258479 1101 38-39 -0.6480741775114787 1101 40-41 -0.2198272795769931 1101 42-43 -1.3591194491653518 1101 44-45 -0.7626941472548516 1101 46-47 0.7040436368109084 1101 48-49 -0.7090520869613144 1101 50-51 -0.956804383596559 1101 52-53 -0.027865793214324697 1101 54-55 -1.4687446761551044 1101 56-57 -0.393480279584999 1101 58-59 -0.055787880983878324 1101 60-61 -0.13980328363950179 1101 62-63 -0.8654721967869676 1101 64-65 -0.6317763078606049 1101 66-67 -1.4384892646117002 1101 68-69 0.5044044819101288 1101 70-71 0.6292266496154255 1101 72-73 -0.18176655316359458 1101 74-75 -1.365832244658698 1101 76 -1.7339858454607828 1102 1 0.5540365653309465 1102 2 -0.3120915167553946 1102 3 -0.24696400624326742 1102 4 -0.34182674807033564 1102 5 1.0044055414205353 1102 6 1.2049486004542977 1102 7 1.991715299944289 1102 8 1.1891516373791582 1102 9 1.1121268003856386 1102 10-11 -0.508669322145419 1102 12-13 -3.5428989184804074 1102 14-15 -0.6891223998727227 1102 16-17 -2.174472246778656 1102 18-19 -0.612219946441801 1102 20-21 -0.11818751763399504 1102 22-23 -0.50819902291353 1102 24-25 0.42740726814856345 1102 26-27 -4.725611516177828 1102 28-29 -0.6227961007265073 1102 30-31 0.13820394770812428 1102 32-33 -6.118975202077017 1102 34-35 -1.1210380839099692 1102 36-37 0.238367478091142 1102 38-39 0.42669706431858856 1102 40-41 0.27762370081515897 1102 42-43 -0.4217338282503178 1102 44-45 -4.575112447908449 1102 46-47 -0.20536812789497105 1102 48-49 0.387810658136722 1102 50-51 -4.101706344380872 1102 52-53 -0.8715259239332767 1102 54-55 -0.010182584651840187 1102 56-57 -1.1913234168399 1102 58-59 0.4655193085586049 1102 60-61 0.3475823372755329 1102 62-63 -4.216452588943831 1102 64-65 -1.524717484331191 1102 66-67 -0.3436526633045105 1102 68-69 -6.1568373481552285 1102 70-71 -0.951230866724444 1102 72-73 -0.36751818715051954 1102 74-75 -4.854198257730594 1102 76 -1.3324172180098017 1103 1 -0.2725375425730867 1103 2 -0.3026732566977479 1103 3 -0.7801511941619133 1103 4 -0.428052542736026 1103 5 -0.1902926277021848 1103 6 -1.7832785744736448 1103 7 -0.7758728947538813 1103 8 0.8821244786895939 1103 9 -0.4863386727601444 1103 10-11 0.24363449793333558 1103 12-13 -1.5273506701955846 1103 14-15 -0.2917692557165097 1103 16-17 -0.3564638840132588 1103 18-19 -0.4997691690293884 1103 20-21 -1.0029965136961856 1103 22-23 -0.7967144290759904 1103 24-25 -0.7138463347418664 1103 26-27 -1.1385941005159097 1103 28-29 -0.2397327304508643 1103 30-31 -1.0383478324242184 1103 32-33 -4.040685915347833 1103 34-35 -1.6325267373423564 1103 36-37 -0.8928386275395113 1103 38-39 0.1921742696934885 1103 40-41 -0.25355398765153225 1103 42-43 -0.2905480205939206 1103 44-45 -5.108035762161688 1103 46-47 -0.5620847275783234 1103 48-49 -1.0071473250565504 1103 50-51 -1.0610900978822748 1103 52-53 -1.2356918801708474 1103 54-55 0.0017729221885787183 1103 56-57 -0.3267515011170907 1103 58-59 -0.18030133854081498 1103 60-61 -0.5139854782564797 1103 62-63 0.059579563047400086 1103 64-65 -0.30842227059352467 1103 66-67 -0.5925058277586999 1103 68-69 -3.4808336133279667 1103 70-71 -0.12317500669927028 1103 72-73 -0.26172514529609714 1103 74-75 -1.9122504641203975 1103 76 -0.4920810835560232 1104 1 -1.3783864038567302 1104 2 -0.3308823664285967 1104 3 -0.04283235358220239 1104 4 -0.3628351514316783 1104 5 -0.10437130265042427 1104 6 -0.22478996163720666 1104 7 1.0320981197388726 1104 8 1.361420544942181 1104 9 -0.6819908466731874 1104 10-11 -1.22569780020331 1104 12-13 -0.5365380407959996 1104 14-15 -0.1804856118241709 1104 16-17 0.24925557768446538 1104 18-19 0.644899568030656 1104 20-21 1.1226504842334215 1104 22-23 -0.07052395288551594 1104 24-25 0.18586898616910474 1104 26-27 0.5970425454468185 1104 28-29 -0.3451933929767428 1104 30-31 -0.18275777031241702 1104 32-33 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1105 14-15 -0.09358797851935208 1105 16-17 -0.2943892754663793 1105 18-19 0.28408548191716676 1105 20-21 -0.46651047955329616 1105 22-23 -0.3737033084600796 1105 24-25 -0.579617897207001 1105 26-27 -0.633442912209297 1105 28-29 -0.4286158338093635 1105 30-31 -0.22403236398222148 1105 32-33 -0.03404701922703168 1105 34-35 0.23233360408658754 1105 36-37 0.22059909329211536 1105 38-39 -0.892625075715074 1105 40-41 0.2489751156325859 1105 42-43 0.8856709699963226 1105 44-45 0.5624555533439484 1105 46-47 -0.22186454682182344 1105 48-49 -0.3219462985381618 1105 50-51 0.15319561640344048 1105 52-53 0.1420743265461546 1105 54-55 -0.27968279990760436 1105 56-57 -0.31202319375665866 1105 58-59 0.0638129174193125 1105 60-61 0.07514681616089547 1105 62-63 0.23352979922101724 1105 64-65 0.6006787819597577 1105 66-67 0.5598540487615544 1105 68-69 0.16929470147101 1105 70-71 0.16960589113239166 1105 72-73 -0.26687633360271334 1105 74-75 -1.413217474199616 1105 76 -0.9206525121274503 1106 1 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0.9859955899303827 1107 40-41 1.2228471390276532 1107 42-43 1.0715782252532584 1107 44-45 0.32625934111723964 1107 46-47 0.016873094175252845 1107 48-49 0.6863742696278337 1107 50-51 0.9002886396592551 1107 52-53 1.053994671901954 1107 54-55 1.1533871067906283 1107 56-57 0.19946545684911143 1107 58-59 0.5163439019618572 1107 60-61 -0.11356297356198297 1107 62-63 -0.6802008789575105 1107 64-65 0.20903764562776672 1107 66-67 1.046433216008456 1107 68-69 0.8866913036155566 1107 70-71 0.23519564186348774 1107 72-73 0.5493574778441577 1107 74-75 0.05889643751184792 1107 76 0.0037660925237119613 1108 1 -0.7861767129628277 1108 2 -0.22781219099000083 1108 3 -0.7770637654451953 1108 4 -0.4777056610390318 1108 5 -0.8641875098350518 1108 6 0.07491764070197604 1108 7 0.7457572676085462 1108 8 -0.4627231476225617 1108 9 -0.12351131450944308 1108 10-11 -0.7095637149902743 1108 12-13 -0.6927097199939922 1108 14-15 -0.33858920413829097 1108 16-17 -1.6550364022654094 1108 18-19 -0.850610039321058 1108 20-21 -0.2886381540289058 1108 22-23 -1.0945423321670553 1108 24-25 -1.4105700280330637 1108 26-27 -1.1487281312449085 1108 28-29 -0.7235872943969603 1108 30-31 0.16039177020554263 1108 32-33 0.36193295065776 1108 34-35 0.320998379798322 1108 36-37 0.2206516198180104 1108 38-39 0.08046383418442815 1108 40-41 -0.19193254273488947 1108 42-43 -0.49104927372675533 1108 44-45 0.38876784104924056 1108 46-47 0.4146869116647096 1108 48-49 -0.517590098657223 1108 50-51 -0.007623097046849381 1108 52-53 0.6121342067856759 1108 54-55 0.13137228290921854 1108 56-57 0.24400510053195745 1108 58-59 -0.84923817338154 1108 60-61 0.5326528567113726 1108 62-63 0.4011944698796981 1108 64-65 0.09722954009261287 1108 66-67 -0.022875229523979357 1108 68-69 0.7138196865884936 1108 70-71 1.1867120297323837 1108 72-73 0.35951999654401234 1108 74-75 0.21872915885007416 1108 76 0.9740843299778135 1109 1 0.3621517189068264 1109 2 0.23583246979490724 1109 3 0.11946464614254282 1109 4 -0.6869474553127688 1109 5 0.07743436759455236 1109 6 0.35372641601211896 1109 7 -0.9502167715355547 1109 8 1.0962127277112437 1109 9 0.29777793945397946 1109 10-11 0.9193785829511043 1109 12-13 0.27651246842502886 1109 14-15 0.030152978872223457 1109 16-17 -0.20193833918864357 1109 18-19 1.056921313144887 1109 20-21 0.7090632285218845 1109 22-23 1.1702550176622353 1109 24-25 0.9239126140248715 1109 26-27 0.46672669078125395 1109 28-29 -0.3847440398250761 1109 30-31 0.2996947482672674 1109 32-33 1.0877383118449302 1109 34-35 0.4129261761678542 1109 36-37 0.308827261233688 1109 38-39 0.872388250234188 1109 40-41 0.8062420845848557 1109 42-43 0.5930192791583515 1109 44-45 0.09106307817867432 1109 46-47 -0.07865385837213523 1109 48-49 -0.2305164414892502 1109 50-51 1.0751609343225148 1109 52-53 0.5976833397336527 1109 54-55 0.27341331999132734 1109 56-57 0.620758641416927 1109 58-59 0.1753874562030191 1109 60-61 0.7390213791736002 1109 62-63 0.022581753116693193 1109 64-65 0.2906514378041365 1109 66-67 0.6322814482977428 1109 68-69 1.0922464857636953 1109 70-71 0.19641354749596474 1109 72-73 0.21098874548765778 1109 74-75 1.1118941522584507 1109 76 0.02154401966445718 1110 1 0.28646866860703923 1110 2 0.4302459979606361 1110 3 -0.08449902024887024 1110 4 -0.29761776012733065 1110 5 -0.1592367270810655 1110 6 0.62665931372511 1110 7 0.6651208940659963 1110 8 1.5964101929753092 1110 9 -1.4428604118905781 1110 10-11 -0.7501543219424391 1110 12-13 -0.1329407323073859 1110 14-15 -0.22655186441216557 1110 16-17 0.4137224513904698 1110 18-19 0.5406035017780688 1110 20-21 0.4069413745025692 1110 22-23 0.09458991875009559 1110 24-25 0.23956981432645819 1110 26-27 -0.9957369576587709 1110 28-29 -0.19314887951918536 1110 30-31 0.09208695018447699 1110 32-33 0.859935202664591 1110 34-35 0.513435995576895 1110 36-37 -0.7065032238128062 1110 38-39 0.45304383491088274 1110 40-41 0.21228452166523937 1110 42-43 0.18150166884707275 1110 44-45 1.003765480074339 1110 46-47 0.53418856434714 1110 48-49 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40-41 -0.8565386889729716 2116 42-43 -1.4405959592324642 2116 44-45 -0.15128475128169683 2116 46-47 -0.7676789582673891 2116 48-49 -0.5263227804736204 2116 50-51 -0.49445539030796937 2116 52-53 -0.4952483435498962 2116 54-55 -0.5015410967367657 2116 56-57 0.27446155486690316 2116 58-59 -0.5228124894849984 2116 60-61 -2.0661343798363703 2116 62-63 0.10253675175889398 2116 64-65 0.08325724918637434 2116 66-67 -0.5581984368712085 2116 68-69 -0.36769842636727645 2116 70-71 -1.8499456098924014 2116 72-73 -0.152862750031602 2116 74-75 -1.0510224012582512 2116 76 -0.987766606087181 2117 1 -0.43521941103220385 2117 2 0.11032767923350306 2117 3 1.0397671245652198 2117 4 0.3132900931867155 2117 5 0.36692680563398383 2117 6 -0.8149726100390318 2117 7 -0.21627109095910413 2117 8 -1.375043903851033 2117 9 0.9481461396281858 2117 10-11 0.8925751939258504 2117 12-13 1.2580232768817545 2117 14-15 0.9651438728464043 2117 16-17 1.0721926292174047 2117 18-19 0.6919478393939933 2117 20-21 0.7043122585321768 2117 22-23 0.827551715738096 2117 24-25 1.169438783951236 2117 26-27 1.2764701384018124 2117 28-29 1.1616966918243001 2117 30-31 1.0046199204899295 2117 32-33 2.048206282388918 2117 34-35 0.9987801630235076 2117 36-37 -0.6281827949861238 2117 38-39 0.8421997950912612 2117 40-41 0.07483025466957827 2117 42-43 0.2980038385058812 2117 44-45 1.1612784554848723 2117 46-47 0.1756418103268942 2117 48-49 -0.37749957554579083 2117 50-51 1.3244284931157644 2117 52-53 1.4511753026760843 2117 54-55 -0.07522869441994828 2117 56-57 0.34003570215016055 2117 58-59 0.010330840477308811 2117 60-61 1.657502652433557 2117 62-63 0.22996159316736708 2117 64-65 1.078223692139396 2117 66-67 -0.23538424178065043 2117 68-69 0.5333542535996258 2117 70-71 1.3478111245012698 2117 72-73 1.5697859582519307 2117 74-75 0.27202163661984 2117 76 -1.146679909387725 2118 1 -0.20576735623768272 2118 2 -0.8725490330952645 2118 3 0.2185897828399348 2118 4 0.44314940455287655 2118 5 -0.18416538244450464 2118 6 1.0634983266510822 2118 7 0.9426515302922844 2118 8 -3.418823985302346 2118 9 -0.5671259818083243 2118 10-11 -0.6236225106280209 2118 12-13 -0.08134177309973012 2118 14-15 -0.7869849683235302 2118 16-17 0.34537848630737145 2118 18-19 -0.5146885915575048 2118 20-21 -2.514495590412654 2118 22-23 -0.5915985989601609 2118 24-25 -0.21430797650785394 2118 26-27 0.5982491091533788 2118 28-29 -0.6028349631183119 2118 30-31 0.2742885617339148 2118 32-33 -0.11941682016569644 2118 34-35 0.924779792790261 2118 36-37 0.9559527103822596 2118 38-39 -0.012668772106074755 2118 40-41 -0.9106380903878062 2118 42-43 -0.3441194491653512 2118 44-45 0.8129815284208242 2118 46-47 -0.32010050733323325 2118 48-49 -1.0032863211955458 2118 50-51 -0.38058816738034196 2118 52-53 -1.2966495769981066 2118 54-55 1.2523814499710184 2118 56-57 0.4649431438384255 2118 58-59 0.5984463532503526 2118 60-61 0.035557076720859726 2118 62-63 0.6680863617715893 2118 64-65 0.22349396240966968 2118 66-67 -0.7572280033504377 2118 68-69 1.474764842270492 2118 70-71 -0.5011337107449307 2118 72-73 -0.7485683549653963 2118 74-75 0.4775010886746358 2118 76 0.5658339743590375 2119 1 -0.056486780698115524 2119 2 -0.16571450072116534 2119 3 0.5712049879731254 2119 4 0.8761165443545487 2119 5 0.4853854633653327 2119 6 1.0192148345258651 2119 7 -0.5962848881405378 2119 8 1.4068128025646551 2119 9 0.08419620368652403 2119 10-11 0.5248803087381084 2119 12-13 0.6540023840070184 2119 14-15 -0.6096565607792925 2119 16-17 -0.06637049288764985 2119 18-19 -0.7250473315925028 2119 20-21 -0.6673246784935465 2119 22-23 0.6251766295057095 2119 24-25 0.20922886969086107 2119 26-27 0.5917110963204024 2119 28-29 1.1942678951356278 2119 30-31 0.1237684372929273 2119 32-33 -0.3073421555730462 2119 34-35 0.2637599600078353 2119 36-37 -0.6863280595963559 2119 38-39 -0.20044827823090117 2119 40-41 -0.36781289108778736 2119 42-43 -1.0824288016833385 2119 44-45 -0.8878740033699621 2119 46-47 -0.2826709914864516 2119 48-49 -0.036438178088413764 2119 50-51 0.02369921352574167 2119 52-53 -1.4813909730704395 2119 54-55 -1.1468262109272906 2119 56-57 -0.0031445481701339872 2119 58-59 0.7934687137403422 2119 60-61 0.3403645820679344 2119 62-63 0.07385634038329414 2119 64-65 0.6339790878228513 2119 66-67 -0.26009597923999905 2119 68-69 -0.706938443749344 2119 70-71 -0.7079196333581947 2119 72-73 -0.9596562414129934 2119 74-75 -0.2796931559296816 2119 76 0.23042662456319718 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 20.0 13 144.0 14 534.0 15 1106.0 16 1742.0 17 2026.0 18 2266.0 19 2094.0 20 1763.0 21 1342.0 22 1036.0 23 879.0 24 724.0 25 725.0 26 785.0 27 883.0 28 997.0 29 1164.0 30 1447.0 31 1779.0 32 2165.0 33 2868.0 34 3919.0 35 5822.0 36 9966.0 37 11273.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.078707623531 22.616588207403392 10.246554001422426 29.058150167643177 2 16.184358763094618 28.9485631652402 31.642313060146964 23.22476501151822 3 14.781994585596342 31.373274369020194 27.7799263506583 26.064804694725158 4 10.519412823056616 28.408804291167122 33.66851070269543 27.40327218308083 5 10.277278001042525 36.15543710379849 31.243799498915436 22.323485396243548 6 20.67057893763347 37.61160901952212 16.811555211783894 24.906256831060517 7 20.652082527618504 31.623816650131996 19.88700374972676 27.83709707252274 8 18.52163239225841 39.06778093524575 17.52618923508937 24.884397437406466 9 19.623009534058617 21.64416270114846 19.572564779472348 39.16026298532058 10-11 19.892888971095154 27.754703973365167 26.293487582182912 26.058919473356763 12-13 19.929881791125087 24.970994266112896 25.430882278757714 29.668241664004302 14-15 19.154714062316085 26.80213885759446 23.009534058616804 31.03361302147265 16-17 20.463755443829765 29.11587160128466 24.065510921289366 26.354862033596206 18-19 19.147147349128147 27.72611861243295 24.68093692724185 28.445797111197052 20-21 18.793193321114494 28.1414470918599 24.65235156630963 28.413008020715978 22-23 21.508802609675303 27.712666677876612 24.195826537303898 26.58270417514419 24-25 20.069445612147096 29.73381984496645 23.683812278253267 26.512922264633183 26-27 18.7318188697012 30.153352053942257 25.38968572917893 25.725143347177614 28-29 20.508314977047636 28.465975013031564 27.040069950059692 23.985640059861108 30-31 19.855055405155454 28.282692404701454 26.966925055909602 24.89532713423349 32-33 18.345916497116242 30.76373358443611 25.911788939146813 24.978560979300834 34-35 19.74155470733635 28.755191605992835 26.032015604244087 25.47123808242673 36-37 19.88700374972676 27.718551899245007 26.17578315481495 26.218661196213283 38-39 19.42795648299171 27.067814565082145 27.913604950311914 25.59062400161423 40-41 18.94284609305376 26.808024078962855 27.41756486354694 26.83156496443645 42-43 17.6623900724723 26.475929444603253 28.31800373291184 27.543676750012615 44-45 17.600174875149232 26.889576432210653 28.697180138218627 26.81306855442148 46-47 17.85408013990012 27.50920616771199 28.247381076491063 26.38933261589682 48-49 17.42950345546569 28.744261909165814 27.056884868255114 26.769349767113383 50-51 17.316002757646583 27.87072690891359 26.512081518723413 28.301188814716415 52-53 16.818281179062065 27.900993761665347 27.36123488759227 27.919490171680316 54-55 16.59548351297271 28.249062568310606 26.675186225219015 28.48026769349767 56-57 17.632123219720537 27.986749844462004 26.276672663987487 28.104454271829965 58-59 17.83053925442653 27.510046913621768 26.219501942123053 28.439911889828657 60-61 17.242017117586723 26.713019791158715 26.14467555615342 29.900287535101143 62-63 17.471540750954244 27.03250323687175 26.241361335777103 29.2545946763969 64-65 18.33078307074036 27.645407005094917 24.522035950295102 29.501773973869618 66-67 17.76580181937415 29.000689411646015 24.183215348657328 29.05029342032251 68-69 18.333305308469676 28.54500512855005 24.34043483378453 28.781254729195744 70-71 19.33295219518757 28.82413277059407 23.97891409258294 27.86400094163542 72-73 20.055152931680986 28.44327487346774 23.478670276269106 28.022901918582164 74-75 18.99749457718888 29.270568848682547 23.72837181147114 28.00356476265743 76 19.760891863261083 27.517613626809705 24.52119520438533 28.20029930554388 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.5 11 1.5 12 0.0 13 2.0 14 3.0 15 3.5 16 6.5 17 8.0 18 11.0 19 15.0 20 26.0 21 36.0 22 40.0 23 57.5 24 86.5 25 102.0 26 133.0 27 184.5 28 261.5 29 318.0 30 365.0 31 489.5 32 696.5 33 826.0 34 1001.5 35 1307.0 36 1542.0 37 1647.0 38 1934.5 39 2496.5 40 3051.0 41 3458.5 42 3586.0 43 3724.5 44 4040.5 45 4262.5 46 4307.0 47 4311.5 48 4228.0 49 3989.5 50 3839.0 51 3542.5 52 3005.0 53 2511.0 54 2258.0 55 2111.5 56 1761.0 57 1377.5 58 1198.0 59 1026.5 60 730.0 61 501.5 62 398.0 63 334.0 64 222.5 65 151.0 66 112.0 67 97.0 68 82.5 69 56.5 70 40.5 71 36.0 72 28.5 73 22.0 74 18.0 75 13.0 76 12.0 77 12.0 78 10.5 79 8.0 80 9.0 81 5.5 82 4.0 83 7.0 84 5.5 85 4.5 86 5.0 87 5.0 88 5.5 89 4.0 90 4.0 91 4.0 92 2.0 93 4.5 94 5.5 95 2.5 96 1.0 97 2.0 98 2.0 99 2.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7011820887491382 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 59471.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.61880580450976 #Duplication Level Percentage of deduplicated Percentage of total 1 82.35876627297539 57.337189554572824 2 8.417264449436031 11.719997982209817 3 3.845131995266043 8.030804930134016 4 1.9322271332995193 5.380773822535353 5 1.137598724730092 3.959913235022112 6 0.7753061372364322 3.2385532444384655 7 0.4661497959085091 2.2716954482016445 8 0.27292708257855713 1.5200686048662373 9 0.23428253991256673 1.467942358460426 >10 0.5603458686568606 5.073060819559113 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.008407459097711489 0.0 2 0.0 0.0 0.0 0.008407459097711489 0.0 3 0.0 0.0 0.0 0.008407459097711489 0.0 4 0.0 0.0 0.0 0.008407459097711489 0.0 5 0.0 0.0 0.0 0.008407459097711489 0.0 6 0.0 0.0 0.0 0.008407459097711489 0.0 7 0.0 0.0 0.0 0.008407459097711489 0.0 8 0.0 0.0 0.0 0.008407459097711489 0.0 9 0.0 0.0 0.0 0.008407459097711489 0.0 10 0.0 0.0 0.0 0.008407459097711489 0.0 11 0.0 0.0 0.0 0.010088950917253788 0.0 12 0.0 0.0 0.0 0.010088950917253788 0.0 13 0.0 0.0 0.0 0.010088950917253788 0.0 14 0.0 0.0 0.0 0.013451934556338384 0.0 15 0.0 0.0 0.0 0.015133426375880681 0.0 16 0.0 0.0 0.0 0.016814918195422978 0.0 17 0.0 0.0 0.0 0.018496410014965277 0.0 18 0.0 0.0 0.0 0.028585360932219066 0.0 19 0.0 0.0 0.0 0.03194834457130366 0.0 20 0.0 0.0 0.0 0.03531132821038826 0.0 21 0.0 0.0 0.0 0.03531132821038826 0.0 22 0.0 0.0 0.0 0.03531132821038826 0.0 23 0.0 0.0 0.0 0.04035580366901515 0.0 24 0.0 0.0 0.0 0.045400279127642044 0.0 25 0.0 0.0 0.0 0.05044475458626894 0.0 26 0.0 0.0 0.0 0.053807738225353535 0.0 27 0.0 0.0 0.0 0.060533705503522724 0.0 28 0.0 0.0 0.0 0.0723041482403188 0.0 29 0.0 0.0 0.0 0.09080055825528409 0.0 30 0.0 0.0 0.0 0.12443039464613005 0.0 31 0.0 0.0 0.0 0.14797128011972221 0.0 32 0.0 0.0 0.0 0.17655664105194127 0.0 33 0.0 0.0 0.0 0.21691244472095644 0.0 34 0.0 0.0 0.0 0.27240167476585225 0.0 35 0.0 0.0 0.0 0.33293538026937497 0.0 36 0.0 0.0 0.0 0.45232129945687816 0.0 37 0.0 0.0 0.0 0.6591447932605808 0.0 38 0.0 0.0 0.0 0.981991222612702 0.0 39 0.0 0.0 0.0 1.3031561601452808 0.0 40 0.0 0.0 0.0 1.664676901346875 0.0 41 0.0 0.0 0.0 2.115316708984211 0.0 42 0.0 0.0 0.0 2.634897681222781 0.0 43 0.0 0.0 0.0 3.159523128919978 0.0 44 0.0 0.0 0.0 3.605118461098687 0.0 45 0.0 0.0 0.0 4.14151435153268 0.0 46 0.0 0.0 0.0 4.6257839955608615 0.0 47 0.0 0.0 0.0 5.115098115047671 0.0 48 0.0 0.0 0.0 5.685123841872509 0.0 49 0.0 0.0 0.0 6.075229944006322 0.0 50 0.0 0.0 0.0 6.53427721074137 0.0 51 0.0 0.0 0.0 7.0740360848144475 0.0 52 0.0 0.0 0.0 7.52972036791041 0.0 53 0.0 0.0 0.0 8.045938356509895 0.0 54 0.0 0.0 0.0 8.61260109969565 0.0 55 0.0 0.0 0.0 9.069966874611154 0.0 56 0.0 0.0 0.0 9.49033982949673 0.0 57 0.0 0.0 0.0 9.920801735299557 0.0 58 0.0 0.0 0.0 10.33276723108742 0.0 59 0.0 0.0 0.0 10.73464377595803 0.0 60 0.0 0.0 0.0 11.153335239024063 0.0 61 0.0 0.0 0.0 11.551848800255586 0.0 62 0.0 0.0 0.0 11.96381429604345 0.0 63 0.0 0.0 0.0 12.389231726387651 0.0 64 0.0 0.0 0.0 12.84996048494224 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCTAAG 20 0.006607158 53.116596 1 ATGGGTT 20 0.006924421 52.49117 7 AGATCTC 20 0.006924421 52.49117 40 TATGGGT 20 0.006924421 52.49117 6 AATTACT 20 0.006924421 52.49117 36 TCTGGCC 20 0.006924421 52.49117 8 GAAACCC 35 1.9254609E-5 50.587234 1 TTATATG 30 5.8391225E-4 46.658817 3 GTTATAT 30 5.8391225E-4 46.658817 2 GATCTCG 35 0.0012472208 39.99327 41 GAAACAC 40 0.0022671947 35.411064 1 GGCCTAT 40 0.002403071 34.994114 11 TTACTAT 50 1.692761E-4 34.99411 3 GGTTATA 45 0.0040381015 31.476503 1 AGCAAAC 45 0.0042795143 31.105879 17 GGTTAGG 65 7.350334E-4 27.23928 1 TAAATAG 70 0.0012141119 24.995794 52 ACAACTC 85 0.0037529203 20.584772 5 ATAACCA 85 0.0037529203 20.584772 26 GATGGGA 90 0.0052244654 19.441174 70 >>END_MODULE