FastQCFastQC Report
Wed 25 May 2016
SRR1780510_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780510_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59471
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT32535.469892888971096No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT30205.07810529501774RNA PCR Primer, Index 35 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC9731.636091540414656No Hit
CTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC5100.857560827966572No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG3550.5969295959375158No Hit
TATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCT2020.3396613475475442No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGT1860.31275747843486745No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC1690.28417211750264837No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC1230.20682349380370266No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGTCTT1180.19841603470599117No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG1050.17655664105194127No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGT950.1597417228565183No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG950.1597417228565183No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGT940.158060231036976No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTCTT930.15637873921743373No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC800.13451934556338382No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC780.13115636192429925No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGTCTT720.12106741100704546No Hit
TTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC660.11097846008979166No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTT150.002214512670.0000129
ACCGGGT150.002214512670.0000127
GTTTTCC150.002214512670.0000132
TTACACA1900.058.9473723
CTATACA800.056.8751
TTTACAC2000.055.9999962
CCGGGTT200.00691981752.528
TCACATC200.00691981752.55
AGGGTAA200.00691981752.528
TAAGGCT200.00691981752.520
GCCAACG200.00691981752.518
CTTTACA2150.052.0930181
TTAATAC352.0634527E-550.03
CTTAATA305.8342825E-446.6666682
ACTGGGC305.8342825E-446.66666824
AGGAGCC350.001246189840.07
TGGGCTT350.001246189840.026
GGATTAA350.001246189840.01
TAATACA501.691088E-435.04
CACCTCG501.691088E-435.036
Produced by FastQC (version 0.11.5)