##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780510_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 59471 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.997528207025276 34.0 34.0 34.0 32.0 34.0 2 33.34707672647173 34.0 34.0 34.0 33.0 34.0 3 33.5007146340233 34.0 34.0 34.0 34.0 34.0 4 33.509946024112594 34.0 34.0 34.0 34.0 34.0 5 33.52856013855492 34.0 34.0 34.0 34.0 34.0 6 37.28721561769602 38.0 38.0 38.0 36.0 38.0 7 36.88816397908224 38.0 37.0 38.0 35.0 38.0 8 36.927544517495924 38.0 37.0 38.0 35.0 38.0 9 36.716147365943065 38.0 37.0 38.0 34.0 38.0 10-11 36.424131089102254 38.0 37.0 38.0 34.0 38.0 12-13 36.25768021388576 38.0 37.0 38.0 34.0 38.0 14-15 36.41122563938727 38.0 37.0 38.0 34.0 38.0 16-17 36.53410906155942 38.0 37.0 38.0 34.0 38.0 18-19 36.57026113567957 38.0 37.0 38.0 34.0 38.0 20-21 36.33770240957777 38.0 37.0 38.0 34.0 38.0 22-23 36.36268937801617 38.0 37.0 38.0 33.5 38.0 24-25 35.55378251584806 38.0 36.0 38.0 31.0 38.0 26-27 35.741386558154396 38.0 36.0 38.0 31.5 38.0 28-29 35.67545526391014 38.0 36.5 38.0 31.0 38.0 30-31 35.31121050596089 38.0 36.0 38.0 29.0 38.0 32-33 35.80007062265642 38.0 36.5 38.0 31.5 38.0 34-35 35.56384624438802 38.0 36.0 38.0 31.0 38.0 36-37 35.68751996771536 38.0 36.5 38.0 31.5 38.0 38-39 34.977392342486255 37.5 35.5 38.0 29.0 38.0 40-41 34.96885877150208 37.0 35.5 38.0 29.0 38.0 42-43 35.625060954078464 37.5 36.5 38.0 32.0 38.0 44-45 35.87578819929041 38.0 36.5 38.0 32.5 38.0 46-47 36.0598947386121 38.0 37.0 38.0 33.5 38.0 48-49 35.82919406097089 38.0 36.5 38.0 32.0 38.0 50-51 35.924778463452775 38.0 37.0 38.0 32.0 38.0 52-53 35.75410704376924 38.0 37.0 38.0 31.5 38.0 54-55 36.00621311227321 38.0 37.0 38.0 34.0 38.0 56-57 36.001706714196835 38.0 37.0 38.0 33.0 38.0 58-59 36.012905449714985 38.0 37.0 38.0 33.5 38.0 60-61 35.77676514603756 38.0 36.5 38.0 32.5 38.0 62-63 36.15537825158481 38.0 37.0 38.0 34.0 38.0 64-65 36.160851507457416 38.0 37.0 38.0 33.5 38.0 66-67 35.18623362647341 38.0 36.0 38.0 29.0 38.0 68-69 33.76763464545745 37.0 34.0 38.0 22.5 38.0 70-71 32.86065477291453 37.0 32.5 38.0 16.0 38.0 72-73 31.86689310756503 37.0 28.5 38.0 11.0 38.0 74-75 32.19863462864253 37.0 31.0 38.0 11.0 38.0 76 31.886247078407962 37.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7278003582418222 1101 2 0.5885433650090306 1101 3 0.17003186828661399 1101 4 0.40459273293576103 1101 5 0.31791073380722423 1101 6 -0.5945001827970913 1101 7 -0.04745152276220921 1101 8 0.441720674672581 1101 9 0.34617407403490574 1101 10-11 0.27194413694685693 1101 12-13 0.9962853645292924 1101 14-15 0.8935204621922352 1101 16-17 -0.18682719447448903 1101 18-19 -0.7945430855095523 1101 20-21 -0.09311488079818986 1101 22-23 0.5679050409419517 1101 24-25 -1.5991939244559816 1101 26-27 -0.2836917860423682 1101 28-29 0.4155144239157451 1101 30-31 0.43709009668283727 1101 32-33 0.167056627676736 1101 34-35 0.3847617162088923 1101 36-37 0.5131291631541117 1101 38-39 0.5261467571044207 1101 40-41 1.3431237856684959 1101 42-43 0.5676932329362216 1101 44-45 0.7850669344979906 1101 46-47 0.5698902998214592 1101 48-49 -1.216221688529501 1101 50-51 -1.507823443702712 1101 52-53 -1.712169221663686 1101 54-55 -0.9643748243183694 1101 56-57 -0.7266493260327991 1101 58-59 -1.4154478876893322 1101 60-61 -1.5452620816278682 1101 62-63 -0.6584122412859656 1101 64-65 -1.2173426061174624 1101 66-67 -1.8984420006851792 1101 68-69 -1.3403560640991685 1101 70-71 -0.5780921222229196 1101 72-73 -1.6992228615512275 1101 74-75 -0.6305516335722743 1101 76 -0.6011473977273454 1102 1 -0.6791277463333358 1102 2 0.04135382252537312 1102 3 0.14467239116243036 1102 4 0.2751809682298827 1102 5 -0.065618677957481 1102 6 0.07020569955584932 1102 7 -0.280000542370054 1102 8 0.2906095635614676 1102 9 0.6336250544270641 1102 10-11 0.5821402153782245 1102 12-13 0.23661216191491974 1102 14-15 -0.26589130251364423 1102 16-17 -0.20022588728495094 1102 18-19 -0.6568306672089008 1102 20-21 0.7312642041691362 1102 22-23 0.706859289307971 1102 24-25 -4.725010917920034 1102 26-27 -0.8634303481338677 1102 28-29 0.1520503716281567 1102 30-31 1.6537567633495058 1102 32-33 0.04509584336302197 1102 34-35 0.9569185789539958 1102 36-37 -2.5326224708328198 1102 38-39 0.12660427344428626 1102 40-41 1.5273067922044419 1102 42-43 1.0364514028708598 1102 44-45 0.9738904639097541 1102 46-47 1.2850536985142753 1102 48-49 -3.388966786568716 1102 50-51 -0.5162548162517311 1102 52-53 -0.25066595368982547 1102 54-55 0.6975206005182315 1102 56-57 1.1429585171044607 1102 58-59 0.3503037462975982 1102 60-61 -6.249575807118063 1102 62-63 -2.9786083197173383 1102 64-65 -0.08302888062726765 1102 66-67 1.1007736855893313 1102 68-69 -0.6772841686743334 1102 70-71 -0.9925365666673613 1102 72-73 -5.358830704688479 1102 74-75 -2.038198692395806 1102 76 -0.5893826918449925 1103 1 0.757075720560664 1103 2 0.4210071331249736 1103 3 -0.09017517105085915 1103 4 0.2166010145092656 1103 5 0.15128547500805212 1103 6 -0.025763122755684265 1103 7 0.5521758064303413 1103 8 0.36031280717775616 1103 9 -0.058008120582073275 1103 10-11 0.21927332949343992 1103 12-13 -0.7346256085555893 1103 14-15 -0.27892260198996155 1103 16-17 0.034601376954078944 1103 18-19 0.5880034983413722 1103 20-21 -0.224729787630487 1103 22-23 0.15767729767073746 1103 24-25 -3.1545769472303107 1103 26-27 -0.8783087632680378 1103 28-29 -0.4952723255666598 1103 30-31 0.4922660801196912 1103 32-33 0.9801829216726006 1103 34-35 0.32666647811365834 1103 36-37 -0.5479474414007584 1103 38-39 -0.2594226010736307 1103 40-41 -0.743874143938136 1103 42-43 -0.13330055588365042 1103 44-45 1.0662056508541013 1103 46-47 0.9386687677303698 1103 48-49 -2.5055177547820833 1103 50-51 0.0031703451171622987 1103 52-53 0.7758432006965705 1103 54-55 -0.013091180426030746 1103 56-57 0.7796653737601673 1103 58-59 -0.4356135191593111 1103 60-61 -4.478719638563682 1103 62-63 -1.7047683489464234 1103 64-65 0.035348905269700026 1103 66-67 -1.1196014209750373 1103 68-69 -0.266960618964589 1103 70-71 -0.054303302347143756 1103 72-73 -4.872349155547084 1103 74-75 -1.2818352774646158 1103 76 -3.013569757975791 1104 1 0.3375622630037327 1104 2 0.2711624126280796 1104 3 0.44812710638185393 1104 4 -0.3085025051594741 1104 5 -0.24208926619277094 1104 6 0.007880769583863412 1104 7 0.20564371533303216 1104 8 0.6279111508630564 1104 9 0.9228407407015737 1104 10-11 -0.434841577338851 1104 12-13 0.5386663169102448 1104 14-15 0.8438776050493786 1104 16-17 0.42745851981122485 1104 18-19 0.8567664382999638 1104 20-21 -0.025972023655327803 1104 22-23 0.3926669457038656 1104 24-25 0.7966394088773541 1104 26-27 1.289522499671918 1104 28-29 1.6723001382014573 1104 30-31 0.6210186681114109 1104 32-33 0.7030090086291167 1104 34-35 0.45464266858984814 1104 36-37 1.4565815441064913 1104 38-39 1.6554324713901352 1104 40-41 1.8465761666208778 1104 42-43 0.25412180436479304 1104 44-45 0.7007812202122778 1104 46-47 1.0551283950595618 1104 48-49 0.25651640670859877 1104 50-51 0.8062241753449086 1104 52-53 -0.020740650235111957 1104 54-55 0.0036013661578238043 1104 56-57 0.20966019777672784 1104 58-59 -0.7162812210226619 1104 60-61 0.47521410884832704 1104 62-63 0.603849663475934 1104 64-65 0.7933716795968238 1104 66-67 -0.43248961973279876 1104 68-69 1.577501078757976 1104 70-71 1.2019078777770815 1104 72-73 0.8423247574963924 1104 74-75 0.5139721759515297 1104 76 -0.030909302489249058 1105 1 0.6211736117348394 1105 2 0.3225952612166125 1105 3 0.2798921477277361 1105 4 -0.03772263632571082 1105 5 -0.1802130186877804 1105 6 0.046018779278760746 1105 7 0.595542489213841 1105 8 -0.35676235925955524 1105 9 0.8305253714400962 1105 10-11 0.31649503515045296 1105 12-13 -0.3159102442531463 1105 14-15 0.22260229851958258 1105 16-17 -0.2044918651331713 1105 18-19 0.45533715401140284 1105 20-21 0.1558072748904351 1105 22-23 0.28331422257868155 1105 24-25 0.6797481913124841 1105 26-27 0.32928226585384124 1105 28-29 0.9289475576482786 1105 30-31 1.1896849070620803 1105 32-33 0.8712083242835291 1105 34-35 -0.5689708187212474 1105 36-37 0.7386780653497098 1105 38-39 0.29718468125611963 1105 40-41 0.255419194850127 1105 42-43 -0.06356425209371963 1105 44-45 0.6266437808053809 1105 46-47 0.6130839125959184 1105 48-49 -0.30865681827001623 1105 50-51 0.6819370353392031 1105 52-53 0.3883697004920066 1105 54-55 0.7463836587155583 1105 56-57 0.5824325102945522 1105 58-59 0.3334942280791324 1105 60-61 1.0037997946196384 1105 62-63 -0.5413064528628198 1105 64-65 0.46998274318393385 1105 66-67 0.8240330492150179 1105 68-69 0.31962397582098845 1105 70-71 0.8860396143040319 1105 72-73 0.9941104717821077 1105 74-75 -0.40358556570800275 1105 76 -0.6358779366495 1106 1 -0.9797813411045766 1106 2 0.028281927100536564 1106 3 0.39957435194674673 1106 4 0.14446201398151715 1106 5 0.1500675965523257 1106 6 0.21399654922905142 1106 7 -0.9205234181870452 1106 8 -0.04272376977186809 1106 9 -1.3402311547232628 1106 10-11 0.19651930034554965 1106 12-13 1.1156971292351727 1106 14-15 -0.4979174463044984 1106 16-17 0.22461071402224064 1106 18-19 0.5523196595884841 1106 20-21 0.19858446560704124 1106 22-23 0.533656674928892 1106 24-25 -0.16291941465206605 1106 26-27 0.9274193250687475 1106 28-29 0.6814621363340407 1106 30-31 0.9053907502776042 1106 32-33 0.2411742747355632 1106 34-35 0.8098597554245757 1106 36-37 0.6307762219776336 1106 38-39 -0.06947415792826206 1106 40-41 1.3508362039691448 1106 42-43 0.9090004224787052 1106 44-45 1.1111453658705415 1106 46-47 1.0138118684489186 1106 48-49 1.3005756970914177 1106 50-51 0.10466021642800882 1106 52-53 0.559791562650048 1106 54-55 0.35438334561627016 1106 56-57 0.31289315762733594 1106 58-59 0.5300423083891062 1106 60-61 0.9922542582414167 1106 62-63 0.8874047521780781 1106 64-65 -1.4000223446795559 1106 66-67 -0.2096838307505422 1106 68-69 0.8880753084498565 1106 70-71 -0.708222841177168 1106 72-73 -0.4666738419433827 1106 74-75 -1.1950614374938446 1106 76 -0.19722582909989228 1107 1 0.637257722582909 1107 2 -0.6844023714250795 1107 3 -0.04384410070563405 1107 4 0.2270733530908018 1107 5 0.09318205163668125 1107 6 0.34232152262926974 1107 7 0.7088275470052352 1107 8 0.10908501575784868 1107 9 -0.5907251507712985 1107 10-11 0.5367115788073207 1107 12-13 -0.21507122461799355 1107 14-15 -0.35082062307908046 1107 16-17 0.4748007125022511 1107 18-19 0.7704569144904454 1107 20-21 0.535489770364606 1107 22-23 -0.10996317611230211 1107 24-25 0.15468204453626555 1107 26-27 -0.5247770573601969 1107 28-29 0.7278787650010159 1107 30-31 0.25840792614020813 1107 32-33 0.7038395734131768 1107 34-35 -0.4681840202252161 1107 36-37 0.21855551974325493 1107 38-39 0.27351109818969377 1107 40-41 0.6133951034979575 1107 42-43 0.4995536980525017 1107 44-45 0.5061522058158232 1107 46-47 0.7137662688137141 1107 48-49 0.3410263734860024 1107 50-51 0.8877579516461225 1107 52-53 0.637481941127021 1107 54-55 0.5665166485498503 1107 56-57 0.76482354218426 1107 58-59 0.7386606394424504 1107 60-61 1.3641952827132187 1107 62-63 0.6675955106520135 1107 64-65 0.7876961535724618 1107 66-67 0.32706187528381037 1107 68-69 0.48309354830393403 1107 70-71 -0.574254912920587 1107 72-73 -0.11635464449696542 1107 74-75 -1.1608617110916555 1107 76 -0.2976590256343208 1108 1 -0.6395680628108096 1108 2 0.47135038255289174 1108 3 -0.15838918434496208 1108 4 0.3367564756258261 1108 5 0.05615634784231105 1108 6 -0.4605820541421224 1108 7 -0.4863988911832635 1108 8 -1.136291021233852 1108 9 -0.5376855750878988 1108 10-11 -0.09519036597711761 1108 12-13 1.0190339025409898 1108 14-15 0.11147367856650447 1108 16-17 -0.28244122956220963 1108 18-19 0.27264989694658226 1108 20-21 0.6548968151082448 1108 22-23 -0.24308911110482256 1108 24-25 0.3379405784679932 1108 26-27 -0.6486040667441131 1108 28-29 -0.9655966871953652 1108 30-31 0.10112518440213591 1108 32-33 0.3184016569165067 1108 34-35 0.09984943550713865 1108 36-37 1.0072812099377302 1108 38-39 0.6085444179231345 1108 40-41 0.7832407447328222 1108 42-43 -0.04610793665441548 1108 44-45 0.5554178116909725 1108 46-47 0.8560891302308207 1108 48-49 1.2076964401254742 1108 50-51 0.883813983197868 1108 52-53 -0.09655518657596218 1108 54-55 -1.0581847041635228E-4 1108 56-57 0.37188868566310873 1108 58-59 -0.3796584140051138 1108 60-61 0.3300595557990391 1108 62-63 0.4355643669011684 1108 64-65 0.37827142897025823 1108 66-67 0.6288094613031205 1108 68-69 1.4412228832692477 1108 70-71 -0.35803364269074933 1108 72-73 -0.9708018089196457 1108 74-75 -0.8386802885430349 1108 76 0.1567473391147587 1109 1 -0.7943191022591378 1109 2 0.42176109140594775 1109 3 0.30637098196677215 1109 4 0.2227044863076344 1109 5 0.20492422128314303 1109 6 -0.158854710735433 1109 7 -0.06352088692406 1109 8 0.5943989020328857 1109 9 0.605403361125461 1109 10-11 0.28269558203356127 1109 12-13 0.4408133028722574 1109 14-15 0.6633277839648741 1109 16-17 0.8880282968549906 1109 18-19 0.8260927526407329 1109 20-21 0.9174631538838867 1109 22-23 0.6690225746606444 1109 24-25 -0.23455037917659638 1109 26-27 0.36929472648171924 1109 28-29 -0.7196108169320468 1109 30-31 0.694219191095172 1109 32-33 0.9894458376960102 1109 34-35 -0.13947720479303172 1109 36-37 1.3984856178747194 1109 38-39 1.0348365451583632 1109 40-41 0.43684247545655097 1109 42-43 1.1400631751327595 1109 44-45 0.8013867803554149 1109 46-47 0.6771350011702069 1109 48-49 1.0306183885417894 1109 50-51 0.14599158519902034 1109 52-53 -0.5155796262879591 1109 54-55 -0.05260218462665733 1109 56-57 0.265932562213834 1109 58-59 0.4914499928501712 1109 60-61 0.9383988046919782 1109 62-63 0.5587939244173086 1109 64-65 0.7733124998555638 1109 66-67 0.06926512840922783 1109 68-69 -0.7558666614016687 1109 70-71 0.5501737737308403 1109 72-73 0.2831278128225705 1109 74-75 0.3703924897417892 1109 76 0.14914161961369388 1110 1 -0.4168373229175941 1110 2 -0.4361357240178876 1110 3 -0.01564100956017711 1110 4 -0.00700146996278761 1110 5 0.21960845637451598 1110 6 -0.007129582383008426 1110 7 0.49006400518810267 1110 8 0.7888842357491868 1110 9 0.8115570968092314 1110 10-11 -0.2434595897612155 1110 12-13 0.6815234597673907 1110 14-15 0.9881581433516615 1110 16-17 0.5439181471404169 1110 18-19 0.1811712002047301 1110 20-21 1.008189467027897 1110 22-23 -0.7771053110249113 1110 24-25 -0.7166887484725493 1110 26-27 1.2862875100238895 1110 28-29 0.7997587303339557 1110 30-31 -0.0015227397560835243 1110 32-33 0.7658972073868853 1110 34-35 0.6310143042006118 1110 36-37 0.9768972790961357 1110 38-39 0.7467885790505875 1110 40-41 -0.46126544828594973 1110 42-43 0.29216528262566754 1110 44-45 0.7960193154503727 1110 46-47 0.5846315006496212 1110 48-49 1.0721219967707043 1110 50-51 -0.3229166114045796 1110 52-53 0.3534829522493652 1110 54-55 -0.1373147829105008 1110 56-57 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-3.2722201332758942 2116 40-41 -0.8194712926312846 2116 42-43 -1.0010315992785408 2116 44-45 -0.15652143239238825 2116 46-47 -0.999281959686364 2116 48-49 0.019639609009651338 2116 50-51 -0.8673536450450001 2116 52-53 0.030313999812825898 2116 54-55 -0.7658737057501384 2116 56-57 0.1647376985757063 2116 58-59 -2.2032778653179648 2116 60-61 -1.04356185791422 2116 62-63 0.8147197497654801 2116 64-65 -1.0026893622695852 2116 66-67 0.02352667940430564 2116 68-69 -1.202660314658452 2116 70-71 0.6196931126764085 2116 72-73 0.04444604225190574 2116 74-75 -0.6968379870398351 2116 76 -1.7461138406803656 2117 1 0.6936451071002736 2117 2 0.5859862873834629 2117 3 0.3789816399761108 2117 4 0.14934159138325498 2117 5 0.2464952086930694 2117 6 0.30321671218008106 2117 7 0.7432334087446435 2117 8 0.43140103996937995 2117 9 -1.2152871131797056 2117 10-11 -0.630658602779171 2117 12-13 -1.2436233112697934 2117 14-15 -0.1702694921456711 2117 16-17 -0.8587450026936736 2117 18-19 -0.7586526745506532 2117 20-21 0.16825498221550816 2117 22-23 0.1408730774716389 2117 24-25 -1.5284176687482187 2117 26-27 -1.456522836270679 2117 28-29 -0.8522024710614247 2117 30-31 0.08868827019882275 2117 32-33 -1.2424410892182394 2117 34-35 0.5282320358435939 2117 36-37 -1.1364142158413273 2117 38-39 0.7430873963738236 2117 40-41 -0.0945931093086756 2117 42-43 -0.08956704103638202 2117 44-45 -0.3942937960956172 2117 46-47 -0.7227581020050167 2117 48-49 -0.6985961177532403 2117 50-51 -0.5405631697301061 2117 52-53 0.4763239290212482 2117 54-55 -0.3218634087932557 2117 56-57 0.11764291141012961 2117 58-59 -1.593849714173345 2117 60-61 -0.9150337711255787 2117 62-63 -0.6516542504183889 2117 64-65 -1.252045802464501 2117 66-67 -0.1245150600459084 2117 68-69 0.5779087760834756 2117 70-71 -0.00699623181196074 2117 72-73 2.066028280001291 2117 74-75 1.4721424303546655 2117 76 1.512551232409642 2118 1 0.10284540328687086 2118 2 0.4994442659099292 2118 3 -0.6323104740557284 2118 4 -0.07603789769486724 2118 5 0.18133415723065127 2118 6 -0.0030587413556517618 2118 7 0.7614673961567107 2118 8 0.331900854852762 2118 9 -1.2280601601993268 2118 10-11 -0.34386667386394976 2118 12-13 -0.8720479688040399 2118 14-15 0.6002321739039473 2118 16-17 -2.867908275555571 2118 18-19 -0.40184038280684575 2118 20-21 0.0358040381207303 2118 22-23 0.7288960319329476 2118 24-25 0.9357159854539248 2118 26-27 -2.1307638581144346 2118 28-29 -0.39052161212029546 2118 30-31 -0.5827297231369784 2118 32-33 -0.22411454349443005 2118 34-35 -0.14582386937669156 2118 36-37 -2.8494834494585035 2118 38-39 -0.01042981947215793 2118 40-41 0.07316883071354141 2118 42-43 0.9360716113145955 2118 44-45 -1.9858339664029074 2118 46-47 0.46777318270434165 2118 48-49 0.6201747078668944 2118 50-51 -0.13714776802703454 2118 52-53 -0.3556827351771972 2118 54-55 -0.3484739234174725 2118 56-57 0.7776299532464819 2118 58-59 -0.015267707509146078 2118 60-61 -0.4269737933395774 2118 62-63 -1.1956194484931828 2118 64-65 -0.1972975610724248 2118 66-67 0.6843057470625666 2118 68-69 -0.5698605686036728 2118 70-71 0.3269078777770815 2118 72-73 1.358975336646978 2118 74-75 0.5163853033646646 2118 76 0.9447985482185999 2119 1 0.6291432839012998 2119 2 -0.25337510021878984 2119 3 -0.37749810773257053 2119 4 -0.9172298090306654 2119 5 -0.25168159233186316 2119 6 -0.4546920293198724 2119 7 -0.5405450479420679 2119 8 -0.08269179535140125 2119 9 -0.6245213696101786 2119 10-11 -0.9548184529812005 2119 12-13 -0.354961637868783 2119 14-15 -0.45710303900680316 2119 16-17 -0.8245969786471505 2119 18-19 -0.046989128675022585 2119 20-21 -1.8147695570571827 2119 22-23 -0.08643548663597755 2119 24-25 0.8750266990452147 2119 26-27 0.1604329141974432 2119 28-29 -0.1302649525830617 2119 30-31 0.8947639575941082 2119 32-33 0.3691189777966457 2119 34-35 0.38370656033360007 2119 36-37 -0.36217059703773913 2119 38-39 -1.3876901733512241 2119 40-41 -0.9194181807583632 2119 42-43 0.228556542288743 2119 44-45 0.7364098601574653 2119 46-47 0.41612531181187506 2119 48-49 -0.3163895542369346 2119 50-51 0.9974283548584424 2119 52-53 -0.07238504900180942 2119 54-55 -0.0770127140066208 2119 56-57 -1.5001505250735647 2119 58-59 -0.5791409332528801 2119 60-61 0.30931825410355174 2119 62-63 -0.24140984320442982 2119 64-65 0.09788521162834485 2119 66-67 0.3757066803699729 2119 68-69 0.05304921168020371 2119 70-71 -0.364638884812841 2119 72-73 0.47252773796915903 2119 74-75 -0.3546043913660384 2119 76 0.12115476054603391 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 2.0 22 11.0 23 17.0 24 45.0 25 99.0 26 236.0 27 345.0 28 581.0 29 981.0 30 1410.0 31 2119.0 32 3078.0 33 4693.0 34 6668.0 35 8176.0 36 11281.0 37 19728.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.42925123169276 23.756116426493584 10.58162802037968 33.23300432143398 2 15.690000168149181 17.279009937616653 46.0106606581359 21.020329236098267 3 14.645793748213414 27.38813875670495 37.00122748902827 20.96484000605337 4 10.428612264801332 24.339594087874765 36.36057910578265 28.871214541541256 5 10.021691244472096 40.24986968438399 37.460274755763315 12.268164315380606 6 20.51083721477695 40.24482520892536 17.080593902910664 22.163743673387028 7 20.58314136301727 36.601032435977196 19.628054009517243 23.187772191488286 8 18.264364143868438 37.2013250155538 23.56274486724622 20.97156597333154 9 18.151704191959105 19.36069681021002 18.0440887155084 44.44351028232248 10-11 18.55526222864926 25.57380908341881 31.38168182811791 24.489246859814028 12-13 22.167947402935884 19.205999562812124 28.270081216054884 30.3559718181971 14-15 22.55132753779153 23.185249953758973 20.536059592070085 33.72736291637941 16-17 24.31689394831094 27.772359637470363 20.589867330295437 27.320879083923256 18-19 22.709387768828503 26.470884969144628 20.975769702880395 29.84395755914647 20-21 20.92112121874527 23.83262430428276 21.192682147601353 34.05357232937062 22-23 22.986833919052984 30.2861899076861 21.100200097526525 25.62677607573439 24-25 24.112592692236554 31.97020396495771 21.40286862504414 22.514334717761596 26-27 25.641909502110273 29.134368011299628 25.2972036791041 19.926518807486 28-29 27.151889156059255 22.906122311714956 27.885019589379702 22.05696894284609 30-31 30.01378823292025 21.234719443089908 30.450976106001242 18.300516217988598 32-33 23.638411999125626 21.587832725193792 33.478502127087154 21.29525314859343 34-35 17.930588017689296 25.37203006507373 37.30978123791428 19.387600679322695 36-37 17.540481915555482 27.32171982983303 28.739217433707186 26.398580820904304 38-39 20.075330833515494 23.692219737350978 28.238973617393352 27.993475811740176 40-41 19.17236972642128 24.600225319903817 31.0991912024348 25.128213751240104 42-43 25.14166568579644 22.927140959459233 32.14760135191943 19.783592002824907 44-45 18.95797951942964 25.84368852045535 29.607707958500782 25.59062400161423 46-47 24.997057389315803 21.064888769316138 27.479780060870002 26.45827378049806 48-49 22.14861024701115 21.037984900203462 28.633283449076018 28.180121403709368 50-51 17.307595298548872 20.936254645121153 33.50456525029006 28.25158480603992 52-53 19.226177464646636 20.790805602730742 37.79405088194246 22.188966050680165 54-55 17.25462830623329 21.217063778984716 34.24778463452775 27.280523280254243 56-57 23.70062719644869 21.107766810714466 30.054144036589264 25.13746195624758 58-59 17.800272401674768 28.358359536580856 33.26074893645642 20.580619125287956 60-61 20.193035260883455 36.27734526071531 24.07475912629685 19.454860352104387 62-63 15.119133695414572 42.672058650434664 22.26127019892048 19.94753745523028 64-65 15.5495956012174 43.823880546821144 20.850498562324493 19.776025289636966 66-67 15.18387113046695 42.66449193724672 21.155689327571423 20.995947604714903 68-69 15.486539657984563 40.70807620520926 21.283482705856635 22.52190143094954 70-71 16.1936069681021 37.99751139210707 21.75345966941871 24.055421970372116 72-73 16.752702998099913 35.134771569336316 22.23604782162735 25.876477610936423 74-75 16.987271106926062 34.27805148727951 22.708547022918737 26.02613038287569 76 17.147853575692356 34.71103563081165 22.38401910174707 25.75709169174892 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 1.5 16 2.5 17 2.0 18 3.5 19 7.5 20 13.0 21 16.0 22 18.5 23 30.5 24 52.5 25 65.0 26 99.0 27 154.0 28 205.0 29 235.0 30 298.5 31 427.0 32 629.0 33 766.0 34 886.0 35 1099.0 36 1301.5 37 1411.0 38 1631.5 39 2325.0 40 3843.5 41 5631.5 42 6374.0 43 6402.0 44 6108.0 45 5212.0 46 4638.0 47 4390.5 48 3871.0 49 3396.0 50 3193.0 51 2832.0 52 2174.5 53 1733.0 54 1588.0 55 1448.5 56 1115.0 57 813.5 58 706.0 59 558.5 60 341.5 61 219.0 62 166.0 63 119.5 64 49.5 65 17.5 66 6.0 67 3.0 68 2.0 69 0.5 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 59471.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.57103462191656 #Duplication Level Percentage of deduplicated Percentage of total 1 61.604774535809014 24.993694405676717 2 17.166777188328915 13.929478233088396 3 8.164787798408488 9.937616653494981 4 4.5963196286472146 7.459097711489633 5 2.586206896551724 5.24625447697197 6 1.6951259946949602 4.126380925156799 7 1.0485742705570293 2.9779220124094095 8 0.7045755968169761 2.286828874577525 9 0.5139257294429709 1.8765448706092047 >10 1.8277519893899203 10.598442938575104 >50 0.04559018567639257 1.410771636595988 >100 0.029011936339522544 2.115316708984211 >500 0.008289124668435012 2.493652368381228 >1k 0.008289124668435012 10.547998183988835 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT 3253 5.469892888971096 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT 3020 5.07810529501774 RNA PCR Primer, Index 35 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 973 1.636091540414656 No Hit CTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 510 0.857560827966572 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 355 0.5969295959375158 No Hit TATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCT 202 0.3396613475475442 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGT 186 0.31275747843486745 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 169 0.28417211750264837 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 123 0.20682349380370266 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGTCTT 118 0.19841603470599117 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 105 0.17655664105194127 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGT 95 0.1597417228565183 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 95 0.1597417228565183 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGT 94 0.158060231036976 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTCTT 93 0.15637873921743373 No Hit CTTAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 80 0.13451934556338382 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 78 0.13115636192429925 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGTCTT 72 0.12106741100704546 No Hit TTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 66 0.11097846008979166 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.010088950917253788 0.0 2 0.0 0.0 0.0 0.010088950917253788 0.0 3 0.0 0.0 0.0 0.010088950917253788 0.0 4 0.0 0.0 0.0 0.010088950917253788 0.0 5 0.0 0.0 0.0 0.011770442736796085 0.0 6 0.0 0.0 0.0 0.011770442736796085 0.0 7 0.0 0.0 0.0 0.013451934556338384 0.0 8 0.0 0.0 0.0 0.013451934556338384 0.0 9 0.0 0.0 0.0 0.013451934556338384 0.0 10 0.0 0.0 0.0 0.013451934556338384 0.0 11 0.0 0.0 0.0 0.015133426375880681 0.0 12 0.0 0.0 0.0 0.018496410014965277 0.0 13 0.0 0.0 0.0 0.018496410014965277 0.0 14 0.0 0.0 0.0 0.021859393654049875 0.0 15 0.0 0.0 0.0 0.02354088547359217 0.0 16 0.0 0.0 0.0 0.02522237729313447 0.0 17 0.0 0.0 0.0 0.026903869112676768 0.0 18 0.0 0.0 0.0 0.03867431184947285 0.0 19 0.0 0.0 0.0 0.04371878730809975 0.0 20 0.0 0.0 0.0 0.04876326276672664 0.0 21 0.0 0.0 0.0 0.04876326276672664 0.0 22 0.0 0.0 0.0 0.04876326276672664 0.0 23 0.0 0.0 0.0 0.053807738225353535 0.0 24 0.0 0.0 0.0 0.053807738225353535 0.0 25 0.0 0.0 0.0 0.060533705503522724 0.0 26 0.0 0.0 0.0 0.06389668914260732 0.0 27 0.0 0.0 0.0 0.07062265642077652 0.0 28 0.0 0.0 0.0 0.0823930991575726 0.0 29 0.0 0.0 0.0 0.10257100099208018 0.0 30 0.0 0.0 0.0 0.13115636192429925 0.0 31 0.0 0.0 0.0 0.15301575557834912 0.0 32 0.0 0.0 0.0 0.19000857560827966 0.0 33 0.0 0.0 0.0 0.2303643792772948 0.0 34 0.0 0.0 0.0 0.28585360932219067 0.0 35 0.0 0.0 0.0 0.3447058230061711 0.0 36 0.0 0.0 0.0 0.4691362176523011 0.0 37 0.0 0.0 0.0 0.6843671705537152 0.0 38 0.0 0.0 0.0 1.0240285181012594 0.0 39 0.0 0.0 0.0 1.3620083738292612 0.0 40 0.0 0.0 0.0 1.7319365741285668 0.0 41 0.0 0.0 0.0 2.2447915790889676 0.0 42 0.0 0.0 0.0 2.794639404079299 0.0 43 0.0 0.0 0.0 3.32935380269375 0.0 44 0.0 0.0 0.0 3.798490020346051 0.0 45 0.0 0.0 0.0 4.356745304434094 0.0 46 0.0 0.0 0.0 4.867918817574952 0.0 47 0.0 0.0 0.0 5.387499789813522 0.0 48 0.0 0.0 0.0 6.001244303946462 0.0 49 0.0 0.0 0.0 6.406483832456155 0.0 50 0.0 0.0 0.0 6.92270182105564 0.0 51 0.0 0.0 0.0 7.512905449714987 0.0 52 0.0 0.0 0.0 7.995493601923626 0.0 53 0.0 0.0 0.0 8.538615459635789 0.0 54 0.0 0.0 0.0 9.130500580114678 0.0 55 0.0 0.0 0.0 9.609725748684232 0.0 56 0.0 0.0 0.0 10.045232129945688 0.0 57 0.0 0.0 0.0 10.512686855778446 0.0 58 0.0 0.0 0.0 10.936422794303105 0.0 59 0.0 0.0 0.0 11.353432765549595 0.0 60 0.0 0.0 0.0 11.793983622269678 0.0 61 0.0 0.0 0.0 12.219401052613879 0.0 62 0.0 0.0 0.0 12.661633401153503 0.0 63 0.0 0.0 0.0 13.108910225151755 0.0 64 0.0 0.0 0.0 13.584772410082225 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTTT 15 0.0022145126 70.00001 29 ACCGGGT 15 0.0022145126 70.00001 27 GTTTTCC 15 0.0022145126 70.00001 32 TTACACA 190 0.0 58.947372 3 CTATACA 80 0.0 56.875 1 TTTACAC 200 0.0 55.999996 2 CCGGGTT 20 0.006919817 52.5 28 TCACATC 20 0.006919817 52.5 5 AGGGTAA 20 0.006919817 52.5 28 TAAGGCT 20 0.006919817 52.5 20 GCCAACG 20 0.006919817 52.5 18 CTTTACA 215 0.0 52.093018 1 TTAATAC 35 2.0634527E-5 50.0 3 CTTAATA 30 5.8342825E-4 46.666668 2 ACTGGGC 30 5.8342825E-4 46.666668 24 AGGAGCC 35 0.0012461898 40.0 7 TGGGCTT 35 0.0012461898 40.0 26 GGATTAA 35 0.0012461898 40.0 1 TAATACA 50 1.691088E-4 35.0 4 CACCTCG 50 1.691088E-4 35.0 36 >>END_MODULE