FastQCFastQC Report
Wed 25 May 2016
SRR1780509_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780509_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407748
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGTT756.184564E-1132.25567632
TGGCATA300.005739809529.33415222
AAACTCG454.0792787E-529.3161427
CGGTGCT300.005767445529.3053443
CTACTGG656.0030106E-727.031179
TCGTTGT756.9037924E-826.43322434
GTAAACA508.2640356E-526.4299736
CGGTAAG508.347412E-526.39100628
CCGGAAT701.148408E-625.0942173
CGGTATG450.001404413524.42111843
GCACTTA450.001404413524.4211181
AAAAGAC1001.7553248E-924.1769051
TTCGGTA551.5974283E-423.98593126
CGGTGGT1650.023.97709743
TTGGCGG651.7937335E-523.71920439
TCCGTAT651.7971535E-523.71336713
GAGGTTT651.8264516E-523.66388744
TATTAAG752.0319148E-623.50198215
AACTCTC752.0362695E-623.49619919
ACGTTCT752.0384505E-623.49330939
Produced by FastQC (version 0.11.5)