FastQCFastQC Report
Wed 25 May 2016
SRR1780509_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780509_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407748
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT291477.148287667873294No Hit
ATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCTT231535.678262063823734RNA PCR Primer, Index 45 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC72501.7780589972237755RNA PCR Primer, Index 45 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC38180.9363626553655688No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG33300.8166808911386445No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC18940.4645025849299077No Hit
TATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCT18640.4571450994241541RNA PCR Primer, Index 45 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC11810.28963967940983154No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG10200.2501545071956208No Hit
CTTATAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG8340.20453809705994877No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC7020.17216516083463315No Hit
CTTAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC5920.14518771398020347RNA PCR Primer, Index 45 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC4750.1164935205077646No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG4700.11526727292347233No Hit
CTTATTACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG4190.1027595475636913No Hit
CTTATCACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC4080.10006180287824834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAA1000.044.033511
CGACATG207.8542717E-443.9957128
CCGTCTT25000.042.2410644
GCCGTCT27250.038.75326543
TTTACAC9100.036.7436682
CTATACA4750.036.617341
CTTTACA9500.035.2268071
TCGACAT250.002350680335.20088227
TTGATAA250.002352095435.1965689
CACATCG250.002352095435.1965687
CGGTATG250.002352095435.19656833
TTACACA9850.034.3925823
CTTTATA2250.033.2412032
TAGCCGA353.215991E-431.4255059
GCTCGCG353.215991E-431.42550529
TCTTTAT2350.030.9171431
TTATACA42550.030.605712
CTTATAC43450.030.3522131
CTTTTGG300.005739901729.3340726
TACTGCC300.005739901729.3340744
Produced by FastQC (version 0.11.5)