##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780508_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 45762 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.401009571259998 32.0 23.0 34.0 12.0 34.0 2 28.174620864472708 34.0 23.0 34.0 11.0 34.0 3 28.354704776889122 34.0 23.0 34.0 11.0 34.0 4 28.720313797473885 34.0 23.0 34.0 11.0 34.0 5 28.712141077750097 34.0 23.0 34.0 11.0 34.0 6 30.8245050478563 36.0 26.0 38.0 11.0 38.0 7 30.534417202045365 36.0 26.0 38.0 11.0 38.0 8 29.346772431274857 34.0 23.0 37.0 11.0 38.0 9 30.06734845505004 35.0 24.0 38.0 12.0 38.0 10-11 30.21831432192649 36.0 24.5 38.0 11.0 38.0 12-13 30.496896988767972 36.5 26.0 38.0 11.0 38.0 14-15 30.112637122503386 36.0 24.0 38.0 11.0 38.0 16-17 29.889908657838383 36.0 23.0 38.0 11.0 38.0 18-19 30.003529128971635 36.0 24.0 38.0 11.0 38.0 20-21 30.09494777326166 36.0 24.0 38.0 11.0 38.0 22-23 29.92241379310345 36.0 23.0 38.0 11.0 38.0 24-25 30.307525894847252 36.5 23.5 38.0 11.0 38.0 26-27 30.315425462173856 37.0 23.5 38.0 11.0 38.0 28-29 30.237282024387046 37.0 23.5 38.0 11.0 38.0 30-31 30.03641667759276 37.0 23.0 38.0 11.0 38.0 32-33 30.034318867182378 36.0 23.5 38.0 11.0 38.0 34-35 29.988778899523624 36.0 23.5 38.0 11.0 38.0 36-37 29.90696429351864 36.0 23.0 38.0 11.0 38.0 38-39 30.173856037760586 37.0 23.5 38.0 11.0 38.0 40-41 29.851525282985882 36.0 23.0 38.0 11.0 38.0 42-43 30.1791442681701 36.5 23.5 38.0 11.0 38.0 44-45 29.336480048948907 35.0 22.0 38.0 11.0 38.0 46-47 29.935765482277873 36.0 23.5 38.0 11.0 38.0 48-49 29.871498186268084 36.0 23.0 38.0 11.0 38.0 50-51 29.878326996197718 35.5 23.5 38.0 11.0 38.0 52-53 29.80576679340938 36.0 23.0 38.0 11.0 38.0 54-55 29.617816091954023 35.5 23.0 38.0 11.0 38.0 56-57 29.50059000917792 35.5 23.0 38.0 11.0 38.0 58-59 29.374885275993183 35.0 22.5 38.0 11.0 38.0 60-61 29.504938595341113 35.5 23.0 38.0 11.0 38.0 62-63 29.493509899042873 35.5 22.5 38.0 11.0 38.0 64-65 29.523786110746908 35.5 23.0 38.0 11.0 38.0 66-67 29.563109129845724 35.5 23.0 38.0 11.0 38.0 68-69 28.94842882741139 34.5 21.5 38.0 11.0 38.0 70-71 28.72634500240374 34.0 20.5 38.0 11.0 38.0 72-73 28.737740920414318 34.0 20.5 38.0 11.0 38.0 74-75 28.592096062235044 34.0 21.0 37.0 11.0 38.0 76 27.822997246623835 32.0 19.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -4.533835462986083 1101 2 -0.8252482429932435 1101 3 -0.9060590079098212 1101 4 -2.4985619187714505 1101 5 -0.3952273941342561 1101 6 -2.4120482488939423 1101 7 -3.7603464486036486 1101 8 -1.7257229574784212 1101 9 -1.433387423170661 1101 10-11 -1.7028294764528908 1101 12-13 -2.1648449798746547 1101 14-15 -1.8749426617121543 1101 16-17 -2.1780099250292295 1101 18-19 -1.461571633497929 1101 20-21 -1.931164235700436 1101 22-23 -0.7187210410443932 1101 24-25 -1.5587892145317461 1101 26-27 -3.4452639036261665 1101 28-29 -2.306693080343912 1101 30-31 -1.7675571613595693 1101 32-33 -0.799564854694701 1101 34-35 -1.7213135075521748 1101 36-37 -0.9311297299738612 1101 38-39 -1.7668369603354108 1101 40-41 -1.5612757869578964 1101 42-43 -1.6423739055264512 1101 44-45 -1.1075013827069 1101 46-47 -0.8873162907437866 1101 48-49 -2.004137114543905 1101 50-51 -3.0785131697912043 1101 52-53 -1.5247522607910327 1101 54-55 -1.8617777767475623 1101 56-57 -1.697643285271262 1101 58-59 -1.3713046338828896 1101 60-61 -1.5868525270788112 1101 62-63 -2.768322241883183 1101 64-65 -2.0337479174783937 1101 66-67 -1.5549942319769237 1101 68-69 -1.4435386841393196 1101 70-71 -1.660946255772327 1101 72-73 -0.9506875959992875 1101 74-75 -3.0565133952370402 1101 76 -2.2684553454449627 1102 1 1.7727322383184578 1102 2 1.306780546930284 1102 3 0.3445032952840599 1102 4 0.6973445139770895 1102 5 1.138960640053778 1102 6 1.2753111753075892 1102 7 1.2027664618776832 1102 8 3.183633767667775 1102 9 2.559640017225199 1102 10-11 1.6218443876946615 1102 12-13 -2.6334684616560295 1102 14-15 -0.1286538627917757 1102 16-17 -1.2301916614214932 1102 18-19 0.3806460902987432 1102 20-21 1.1860197499046023 1102 22-23 0.8742839072237132 1102 24-25 1.0822364264938926 1102 26-27 -2.2701626890512685 1102 28-29 1.0898431319817732 1102 30-31 1.4520784661707964 1102 32-33 -4.872664269899381 1102 34-35 0.7191948145350935 1102 36-37 1.7909800316095854 1102 38-39 1.1392584062862738 1102 40-41 1.3983508437213708 1102 42-43 1.3069063463853787 1102 44-45 -3.391464495617381 1102 46-47 0.4520899170834731 1102 48-49 1.3225610411016184 1102 50-51 -3.8634884284506725 1102 52-53 -0.3782160484653474 1102 54-55 1.5176644184841095 1102 56-57 -0.014894747259564411 1102 58-59 1.5499729189736122 1102 60-61 0.8762603834025207 1102 62-63 -2.7882277749466127 1102 64-65 0.9605165899440067 1102 66-67 1.1661033838575356 1102 68-69 -4.8027379643912305 1102 70-71 0.983678125694162 1102 72-73 0.9063749321158774 1102 74-75 -4.382874168966236 1102 76 -0.5343122955124393 1103 1 0.48997406082343886 1103 2 0.2211176717937242 1103 3 0.81073297204005 1103 4 0.6004355749628871 1103 5 0.9919906509785257 1103 6 -0.1714467451345456 1103 7 1.5278741027747955 1103 8 1.9346780450278445 1103 9 1.2169885166789634 1103 10-11 0.5546893205395911 1103 12-13 -0.453692097669137 1103 14-15 0.4596438044532576 1103 16-17 0.032516390760246594 1103 18-19 1.227024857730143 1103 20-21 0.27560268159279744 1103 22-23 0.6114794602087414 1103 24-25 1.5138924897289066 1103 26-27 0.3172674246946343 1103 28-29 1.2070913808089685 1103 30-31 1.0162774251065976 1103 32-33 -3.4887879123388146 1103 34-35 0.28557872302426546 1103 36-37 0.5369153828080968 1103 38-39 1.0721354707422819 1103 40-41 1.696243010034589 1103 42-43 1.3707839892103912 1103 44-45 -2.827426194736976 1103 46-47 0.31945062654944323 1103 48-49 2.044108499491184 1103 50-51 1.252313897877972 1103 52-53 0.2803855838204967 1103 54-55 0.9765680879140923 1103 56-57 1.0272940580871328 1103 58-59 1.0986201781471863 1103 60-61 1.033448224800889 1103 62-63 -0.11606660278543757 1103 64-65 1.5746480724965437 1103 66-67 1.5653065199027765 1103 68-69 -2.5663457016831757 1103 70-71 0.8265224159068723 1103 72-73 0.7955530055044768 1103 74-75 -0.5477414654124786 1103 76 1.3079857573119291 1104 1 -1.6853874198011987 1104 2 -0.004330564180829555 1104 3 -0.5059240551432929 1104 4 -0.9889802723476997 1104 5 0.5124649135580519 1104 6 0.18471288470929537 1104 7 2.0061852652964873 1104 8 1.262266246300257 1104 9 -1.2386505810653965 1104 10-11 -0.2046513388010638 1104 12-13 -1.319365897553464 1104 14-15 0.684166650028736 1104 16-17 -1.2002771315069616 1104 18-19 0.7310734407260959 1104 20-21 0.6441394080242588 1104 22-23 0.09308732602713121 1104 24-25 -0.4228917786343125 1104 26-27 -0.08811140699998532 1104 28-29 -0.8802423381036988 1104 30-31 -1.2056138415215116 1104 32-33 -1.761980509215622 1104 34-35 -1.1722581769179001 1104 36-37 -0.909874669245113 1104 38-39 -1.1855279185000533 1104 40-41 -0.053785908415381556 1104 42-43 0.4205815600605902 1104 44-45 -3.1423191964720836 1104 46-47 -0.2504741854806305 1104 48-49 -1.0026526341120565 1104 50-51 0.25873379377155103 1104 52-53 -0.221378441627742 1104 54-55 -0.48404498322529577 1104 56-57 -0.4012195335843529 1104 58-59 -0.6312236622229683 1104 60-61 -0.08442337728124016 1104 62-63 -0.09463803135686888 1104 64-65 -0.7638423844149678 1104 66-67 0.12294099069514175 1104 68-69 -2.388208049861312 1104 70-71 -1.1154671734511368 1104 72-73 -0.40858233284138734 1104 74-75 -1.5961220322140974 1104 76 -0.3086712698714109 1105 1 -1.8579981424119225 1105 2 -1.7518830117332769 1105 3 -1.4826140318332683 1105 4 -0.8127564961239209 1105 5 -0.6679546668615295 1105 6 0.2207268707232828 1105 7 -1.092882333771115 1105 8 -0.1471743131403045 1105 9 -2.167438459853276 1105 10-11 -1.5632760474257736 1105 12-13 -3.361615314802883 1105 14-15 -2.076497685635598 1105 16-17 -1.429006735236566 1105 18-19 -1.694625859973204 1105 20-21 -1.7625039486190985 1105 22-23 -2.0119826040427995 1105 24-25 -2.005758911501445 1105 26-27 -2.218589262477842 1105 28-29 -2.8531444360057954 1105 30-31 -1.9214646573723257 1105 32-33 -1.5443814416165544 1105 34-35 -1.8710344006941213 1105 36-37 -1.291576300946744 1105 38-39 -0.8457843287564621 1105 40-41 -2.0222008268303036 1105 42-43 -1.324989535510504 1105 44-45 -0.9510021801550685 1105 46-47 -1.1732014582079024 1105 48-49 -1.4409393473987677 1105 50-51 -1.5789118938741389 1105 52-53 -2.4196884649377637 1105 54-55 -1.9271685263488365 1105 56-57 -2.3204503028151215 1105 58-59 -1.558904314903625 1105 60-61 -0.9118126546705163 1105 62-63 -0.9461531828720204 1105 64-65 -0.643795764368349 1105 66-67 -1.7617326689785173 1105 68-69 -1.3765530382063034 1105 70-71 -1.1607468937308596 1105 72-73 -1.1616126358716947 1105 74-75 -2.083825994918058 1105 76 -2.0914218526219948 1106 1 0.6108294780550416 1106 2 0.4195029793248466 1106 3 -0.6723805494686381 1106 4 -0.08771923829977979 1106 5 0.4408381796362342 1106 6 -0.8318066613184456 1106 7 0.11526471044150455 1106 8 0.5834516183053005 1106 9 1.5187264681149308 1106 10-11 0.38841297393537744 1106 12-13 0.699549613165324 1106 14-15 0.10327131585471605 1106 16-17 0.7322575469293575 1106 18-19 -0.36917876608348976 1106 20-21 0.15813688595616426 1106 22-23 -0.0054624848177624585 1106 24-25 0.25132007508574006 1106 26-27 0.94789111506811 1106 28-29 -0.19833817669134035 1106 30-31 -0.13619391718355445 1106 32-33 0.12414811625726685 1106 34-35 -1.2218168150011266 1106 36-37 0.660112720414407 1106 38-39 -0.4222870609969007 1106 40-41 0.039026197511478955 1106 42-43 0.10633187531910693 1106 44-45 0.9272268312706657 1106 46-47 -0.03572008711997299 1106 48-49 -0.26154292414988234 1106 50-51 -0.2594881482208855 1106 52-53 -0.018856373531903614 1106 54-55 0.08127658045692243 1106 56-57 0.9828283857094675 1106 58-59 0.3016893264277236 1106 60-61 -0.2658861767768279 1106 62-63 0.5947062309382147 1106 64-65 0.3805460140717898 1106 66-67 1.2426761735450818 1106 68-69 1.1392194406809324 1106 70-71 0.40836637005453724 1106 72-73 -0.09218889021844134 1106 74-75 -0.49359996411826046 1106 76 -0.0029966166557748863 1107 1 -0.0032270270025094305 1107 2 -0.6335722444863379 1107 3 -0.1837745734193419 1107 4 -0.07097699902527665 1107 5 -0.2852983161200733 1107 6 -0.5979385064527349 1107 7 -0.5961060603990944 1107 8 1.3562105998190752 1107 9 0.9542572203530426 1107 10-11 -0.08310196618786492 1107 12-13 -0.22130261331996692 1107 14-15 -0.3618780853777004 1107 16-17 0.24789526347394641 1107 18-19 -0.13635700117243132 1107 20-21 0.06808921708045546 1107 22-23 -0.368123803269107 1107 24-25 -0.14744169680125196 1107 26-27 -0.5347562515171695 1107 28-29 -1.0368162606350637 1107 30-31 -0.05992371604415325 1107 32-33 -0.07597396257077804 1107 34-35 0.0648940325747347 1107 36-37 0.20987437603584524 1107 38-39 0.2801021960662311 1107 40-41 0.225864937192906 1107 42-43 0.0639530821555212 1107 44-45 -0.2458925734497157 1107 46-47 -0.29231815711183273 1107 48-49 0.30092074286557846 1107 50-51 0.5592247921348878 1107 52-53 0.005762856790152426 1107 54-55 0.1634181918974562 1107 56-57 0.0359326759082812 1107 58-59 0.41667595588941353 1107 60-61 0.020650272473261566 1107 62-63 -0.8602408682363034 1107 64-65 0.6503692904350054 1107 66-67 0.21320285648892323 1107 68-69 0.4696752016389567 1107 70-71 0.8304684512078886 1107 72-73 0.0066658941089663415 1107 74-75 -0.742030379186275 1107 76 -0.8354579583602266 1108 1 -0.20648299089677025 1108 2 -0.5927921026423668 1108 3 0.8355677863485518 1108 4 0.7857283523609269 1108 5 0.20325745435059162 1108 6 -1.127757977761565 1108 7 0.39954190865313066 1108 8 -0.12444704041303112 1108 9 -0.5765293689441862 1108 10-11 0.41778455863482833 1108 12-13 0.1838392306516603 1108 14-15 -0.1598310189689336 1108 16-17 -1.0880976443274726 1108 18-19 0.021283230935882358 1108 20-21 -0.10341303952818848 1108 22-23 -0.583952301012495 1108 24-25 -0.48681951756205066 1108 26-27 -0.17692259581117398 1108 28-29 0.34382526096390365 1108 30-31 0.5747474638397918 1108 32-33 1.0730428008076878 1108 34-35 0.3600262053664842 1108 36-37 0.5758479414774982 1108 38-39 0.36254900457687 1108 40-41 0.5838597792303055 1108 42-43 0.23561652509555842 1108 44-45 0.30657357742068925 1108 46-47 -0.04062570063214466 1108 48-49 -0.3273029837624044 1108 50-51 -0.7607300756923188 1108 52-53 -0.5371127073620059 1108 54-55 0.20540723122692128 1108 56-57 0.03561030324548753 1108 58-59 -0.9111770421763516 1108 60-61 -0.040600533458395205 1108 62-63 0.5689983322794951 1108 64-65 0.7955981750255923 1108 66-67 0.9352370279911817 1108 68-69 0.9757196890664233 1108 70-71 0.7975864396024761 1108 72-73 0.012629788370730921 1108 74-75 1.533598247506184 1108 76 0.7419114807113374 1109 1 0.2879765296821546 1109 2 0.36616312123823036 1109 3 0.7340300206316783 1109 4 -0.16418119083219906 1109 5 0.2316382775075354 1109 6 1.1437255138711784 1109 7 -0.8382464224187842 1109 8 0.7615773829248234 1109 9 0.8419464338599774 1109 10-11 1.4582074442069697 1109 12-13 0.9966501759252928 1109 14-15 1.681813033496013 1109 16-17 0.21476305218994085 1109 18-19 0.3849195945722492 1109 20-21 1.1265733919507817 1109 22-23 1.386083881710256 1109 24-25 1.0444446163140206 1109 26-27 1.1622904299690155 1109 28-29 1.7813535287160462 1109 30-31 0.9586800735186713 1109 32-33 1.980154967248211 1109 34-35 0.9547797105677489 1109 36-37 1.0172206235218226 1109 38-39 1.6629221389052304 1109 40-41 1.665157834408955 1109 42-43 1.6270683568458963 1109 44-45 0.6674397013465132 1109 46-47 0.36445118765369955 1109 48-49 0.7831108561290456 1109 50-51 1.4181482599024342 1109 52-53 1.234936139313703 1109 54-55 0.7586071407701134 1109 56-57 0.989251189722193 1109 58-59 1.462748783242013 1109 60-61 1.4384813299518235 1109 62-63 0.14566047610581734 1109 64-65 0.8793837923932983 1109 66-67 0.5329869149201691 1109 68-69 1.9929630178769173 1109 70-71 0.8811459149529952 1109 72-73 -0.14589576567721352 1109 74-75 2.0288492651452614 1109 76 -0.1642395821561422 1110 1 -0.23642527083904952 1110 2 -0.3427921026423668 1110 3 -0.5468275960468318 1110 4 -0.024877708245135466 1110 5 -0.5936400925469556 1110 6 -0.38280848281207014 1110 7 0.8169300760412952 1110 8 1.3979194819534904 1110 9 -0.6576982001130176 1110 10-11 -0.5365805207302472 1110 12-13 -0.8816369598245295 1110 14-15 -0.2736477577856711 1110 16-17 -0.2845262157560491 1110 18-19 0.039681499334154324 1110 20-21 0.34536040924525935 1110 22-23 0.9005354334752411 1110 24-25 0.044385388642854906 1110 26-27 -1.1679038367924157 1110 28-29 -0.7703522282135893 1110 30-31 -1.1422438781515467 1110 32-33 0.8693992221641125 1110 34-35 0.7640666094068891 1110 36-37 -0.3442458536163002 1110 38-39 -0.7545144874866239 1110 40-41 0.530829476200001 1110 42-43 -0.3506028111237782 1110 44-45 1.147302293149405 1110 46-47 0.5598432748368332 1110 48-49 1.320424288964869 1110 50-51 -0.11715143211367618 1110 52-53 -0.8892050594543583 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-0.3780217982710994 2116 54-55 -1.062017011197323 2116 56-57 -1.9477230300878468 2116 58-59 -1.5854194664187737 2116 60-61 -1.5102975031553676 2116 62-63 -0.7400925768114135 2116 64-65 0.03196181138923038 2116 66-67 -0.47915691140276095 2116 68-69 -0.48715909881236286 2116 70-71 -2.5402923482763136 2116 72-73 -1.126764151023206 2116 74-75 -2.2618562979483627 2116 76 -2.7338460950462355 2117 1 -0.07310795614558785 2117 2 0.9232629432291937 2117 3 0.41327142827237395 2117 4 0.3803210685132754 2117 5 0.7378001288015241 2117 6 -0.7732264236887296 2117 7 -2.4772304016054214 2117 8 -1.7562235208133643 2117 9 0.9521751070079958 2117 10-11 0.20409954334430935 2117 12-13 0.38758540802169605 2117 14-15 -0.310963907487146 2117 16-17 1.247147003990566 2117 18-19 0.2564792275997725 2117 20-21 -0.015423047868470263 2117 22-23 0.2341600148062426 2117 24-25 1.2638407548872088 2117 26-27 1.3072308654065061 2117 28-29 0.26831094347710405 2117 30-31 0.32116809214115705 2117 32-33 1.395429511955868 2117 34-35 0.49520830155775286 2117 36-37 -1.1480539211858343 2117 38-39 0.33387927980622933 2117 40-41 -0.2810971293045874 2117 42-43 -0.8972024907509812 2117 44-45 -0.23099244982423528 2117 46-47 0.7761313191065291 2117 48-49 0.8135435550199155 2117 50-51 1.0337514366791005 2117 52-53 1.6397013043143893 2117 54-55 0.5358562164924194 2117 56-57 0.23230199076285984 2117 58-59 -0.13108251896256107 2117 60-61 1.4500861498888362 2117 62-63 0.6709582989183609 2117 64-65 0.4642804102215905 2117 66-67 -0.25175907987648927 2117 68-69 0.6791878569841323 2117 70-71 1.2057043156102587 2117 72-73 0.9349956488099842 2117 74-75 -0.11944595599673846 2117 76 -0.8325950525107899 2118 1 -1.2116236835374643 2118 2 -0.5392206740709398 2118 3 -2.0656768023960375 2118 4 -0.4474967558641829 2118 5 -0.6630845369913985 2118 6 -0.3758640383676273 2118 7 0.8784380125492319 2118 8 -4.90366781963381 2118 9 -1.8690077239225396 2118 10-11 -1.3604892508889783 2118 12-13 0.6912797068421384 2118 14-15 -0.8277152181031333 2118 16-17 -0.21607383480366593 2118 18-19 -1.0124018339991814 2118 20-21 -2.8148578447229937 2118 22-23 -0.7165280585882527 2118 24-25 -0.6242654050079395 2118 26-27 -0.29786415425273205 2118 28-29 0.13589777178641071 2118 30-31 -0.7062319733896416 2118 32-33 -0.20401347624859056 2118 34-35 0.5924396252799013 2118 36-37 -0.8075395044099487 2118 38-39 0.3580847188625853 2118 40-41 -1.9647062380857143 2118 42-43 -0.9076464619174303 2118 44-45 -0.7296818338347215 2118 46-47 -0.9272598997663444 2118 48-49 -1.8760042824637004 2118 50-51 -0.7312387337009802 2118 52-53 -1.5776971229464252 2118 54-55 -0.623055972236287 2118 56-57 0.3893711257563055 2118 58-59 -0.01439673914604711 2118 60-61 -1.9912228278306898 2118 62-63 -0.6428523170711529 2118 64-65 -1.5238011756237597 2118 66-67 -1.7208776906235421 2118 68-69 0.10870129079802382 2118 70-71 -1.2066072833412456 2118 72-73 -2.07733233284139 2118 74-75 -0.8158335706756361 2118 76 -0.7202097314098737 2119 1 0.3823048878911095 2119 2 1.4172078973576347 2119 3 0.9825374833182465 2119 4 1.3717889584215328 2119 5 0.3547726058657439 2119 6 0.9316359616323737 2119 7 -0.47227627316505405 2119 8 0.4691893232233326 2119 9 -0.008650581065396068 2119 10-11 1.4061178919681652 2119 12-13 1.507172563984998 2119 14-15 0.14532049618258114 2119 16-17 0.3335690223391907 2119 18-19 0.3399195945722475 2119 20-21 -0.5143221304372787 2119 22-23 1.6134719414117455 2119 24-25 0.9878774521349172 2119 26-27 1.1103501314615514 2119 28-29 1.6232192003578447 2119 30-31 1.308232312324641 2119 32-33 1.2295579523228426 2119 34-35 1.748511053851331 2119 36-37 2.312817638447193 2119 38-39 0.6575490045768717 2119 40-41 -0.6991705237999959 2119 42-43 0.0528892523682849 2119 44-45 -0.11539611954900764 2119 46-47 0.9995258145193695 2119 48-49 0.19542428896486896 2119 50-51 1.6841184091561665 2119 52-53 -0.4848399800892764 2119 54-55 -0.33519882937914147 2119 56-57 1.9695496971848776 2119 58-59 2.221853260853951 2119 60-61 1.8728843150264538 2119 62-63 1.3353619686431308 2119 64-65 0.2042345386619573 2119 66-67 1.2094794522336052 2119 68-69 1.0610227193694541 2119 70-71 0.2310712880873247 2119 72-73 0.016417667158609817 2119 74-75 1.245416429324365 2119 76 0.5197902685901248 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 13.0 13 85.0 14 378.0 15 953.0 16 1557.0 17 2060.0 18 2065.0 19 2043.0 20 1647.0 21 1251.0 22 977.0 23 759.0 24 630.0 25 639.0 26 618.0 27 705.0 28 829.0 29 911.0 30 1116.0 31 1345.0 32 1774.0 33 2216.0 34 3000.0 35 4479.0 36 6828.0 37 6882.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.9984165732005 21.389457016560005 9.49176398143872 37.120362428800775 2 16.478737817403086 30.47506664918491 28.40784930728552 24.63834622612648 3 14.686858091866615 25.4971373628775 26.148332677767584 33.66767186748831 4 10.458458983436039 20.211092172544905 31.137188059962412 38.19326078405664 5 10.076045627376425 39.58087496175867 29.550718937109394 20.792360473755515 6 19.680083912416414 35.14925046982212 15.858135570997772 29.31253004676369 7 20.497355884795248 36.67234823652812 18.21598706350247 24.614308815174162 8 17.639089200646822 33.90804597701149 17.160526200777937 31.292338621563744 9 19.71941785761112 22.56020278833967 17.916612036187228 39.80376731786198 10-11 18.903238494821032 27.10327345832787 25.66758445872121 28.32590358812989 12-13 18.925090686595865 24.998907390411258 25.308115904025176 30.767886018967705 14-15 19.180761330361435 26.862899348804685 22.494646213015166 31.461693107818718 16-17 20.598968576548227 28.660242122284863 23.404790000437046 27.335999300729863 18-19 18.68471657707268 27.5097242253398 23.52169922643241 30.28385997115511 20-21 18.214894453913725 27.49005725274245 24.576067479568202 29.71898081377562 22-23 21.497328540366905 27.551544420528174 23.200725500693814 27.75040153841111 24-25 19.53476683711376 29.977929286307415 23.200472007342338 27.286831869236483 26-27 18.183208775840217 30.824701717582276 24.884183383593374 26.107906122984136 28-29 19.602508631615752 27.55452121847821 27.795987937590137 25.046982212315893 30-31 19.953236309601852 27.795987937590137 27.079236047375556 25.171539705432455 32-33 17.600847865040866 30.719811197063063 26.041256938070887 25.638083999825184 34-35 18.564529522311087 29.66762816310476 25.587823958743062 26.18001835584109 36-37 18.937109392072024 28.060399458065643 26.34281718456361 26.65967396529872 38-39 19.07040776189852 26.475022944801363 28.388182334688167 26.066386958611947 40-41 18.14059700187929 26.194222280494735 27.55233599930073 28.11284471832525 42-43 16.545387002316332 25.903588129889428 28.88422708797692 28.666797779817315 44-45 16.64153664612561 26.552598225602026 29.523403697390847 27.282461430881515 46-47 16.821817228267996 27.70639395131332 28.30186617717757 27.16992264324112 48-49 16.255845461299767 28.769503081159044 26.987456841921244 27.98719461561995 50-51 16.48857130370176 28.28875486211267 26.22153752021328 29.001136313972292 52-53 16.004545255889166 27.715134828023253 27.362221930859665 28.91809798522792 54-55 15.38940605742756 27.93584196494908 26.362484157160964 30.31226782046239 56-57 16.94309689261833 27.778506184170272 26.072942616144402 29.205454307066997 58-59 16.54429439272759 27.494427691097417 25.938551636729162 30.022726279445827 60-61 15.838468598400421 26.35265067086229 26.288186705126527 31.52069402561077 62-63 16.94418950220707 26.96669725973515 25.29391197937153 30.795201258686244 64-65 17.576810454088545 27.2059787596696 23.98824352082514 31.228967265416724 66-67 16.678685372142827 28.799003540055068 23.52169922643241 31.0006118613697 68-69 17.967964686858092 28.439534985359032 23.612385822297977 29.9801145054849 70-71 19.000480748219044 28.241772649796776 23.640793671605262 29.116952930378915 72-73 19.71395480966741 28.48214675931996 23.14693413749399 28.656964293518637 74-75 18.822385385254144 29.19343560159084 22.811502993750274 29.172676019404747 76 19.105371268738256 27.54687295135702 23.563218390804597 29.784537389100123 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 1.0 12 0.0 13 0.5 14 2.0 15 2.5 16 2.5 17 3.0 18 4.0 19 10.0 20 16.0 21 17.0 22 26.5 23 37.5 24 50.5 25 62.0 26 88.5 27 135.5 28 187.5 29 219.0 30 257.0 31 351.0 32 468.0 33 529.0 34 625.0 35 871.5 36 1129.0 37 1236.0 38 1389.0 39 1786.0 40 2185.5 41 2467.5 42 2594.0 43 2754.5 44 3007.5 45 3160.5 46 3221.0 47 3191.0 48 3134.0 49 3051.5 50 2996.0 51 2838.0 52 2466.5 53 2120.0 54 1987.0 55 1830.0 56 1489.5 57 1176.0 58 1046.0 59 904.0 60 665.0 61 511.5 62 455.0 63 396.5 64 274.0 65 169.5 66 126.0 67 123.0 68 102.5 69 72.5 70 46.5 71 30.0 72 26.0 73 25.0 74 24.0 75 20.0 76 18.0 77 16.0 78 12.5 79 9.0 80 7.5 81 5.5 82 5.5 83 6.0 84 4.0 85 4.5 86 4.5 87 2.0 88 2.0 89 1.5 90 2.0 91 3.0 92 3.0 93 3.0 94 3.0 95 2.5 96 2.0 97 2.5 98 3.5 99 3.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6358987806476989 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010926095887417507 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 45762.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.53157641711464 #Duplication Level Percentage of deduplicated Percentage of total 1 85.11555806087937 65.99143394082427 2 8.255355129650507 12.801013941698352 3 3.413190529875987 7.938901271797562 4 1.4853438556933485 4.6064420261352215 5 0.7609921082299886 2.950045889602727 6 0.39177001127395716 1.8224727940212404 7 0.2452085682074408 1.3307984790874523 8 0.12401352874859076 0.7691971504741926 9 0.07328072153325818 0.5113412875311394 >10 0.13528748590755355 1.2783532188278486 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004370438354967003 0.0 2 0.0 0.0 0.0 0.004370438354967003 0.0 3 0.0 0.0 0.0 0.008740876709934006 0.0 4 0.0 0.0 0.0 0.008740876709934006 0.0 5 0.0 0.0 0.0 0.008740876709934006 0.0 6 0.0 0.0 0.0 0.008740876709934006 0.0 7 0.0 0.0 0.0 0.008740876709934006 0.0 8 0.0 0.0 0.0 0.008740876709934006 0.0 9 0.0 0.0 0.0 0.010926095887417508 0.0 10 0.0 0.0 0.0 0.01529653424238451 0.0 11 0.0 0.0 0.0 0.01529653424238451 0.0 12 0.0 0.0 0.0 0.01529653424238451 0.0 13 0.0 0.0 0.0 0.019666972597351513 0.0 14 0.0 0.0 0.0 0.019666972597351513 0.0 15 0.0 0.0 0.0 0.024037410952318518 0.0 16 0.0 0.0 0.0 0.028407849307285522 0.0 17 0.0 0.0 0.0 0.028407849307285522 0.0 18 0.0 0.0 0.0 0.03059306848476902 0.0 19 0.0 0.0 0.0 0.03059306848476902 0.0 20 0.0 0.0 0.0 0.03496350683973602 0.0 21 0.0 0.0 0.0 0.03496350683973602 0.0 22 0.0 0.0 0.0 0.03496350683973602 0.0 23 0.0 0.0 0.0 0.037148726017219524 0.0 24 0.0 0.0 0.0 0.04588960272715353 0.0 25 0.0 0.0 0.0 0.04588960272715353 0.0 26 0.0 0.0 0.0 0.048074821904637036 0.0 27 0.0 0.0 0.0 0.07648267121192255 0.0 28 0.0 0.0 0.0 0.10926095887417508 0.0 29 0.0 0.0 0.0 0.1529653424238451 0.0 30 0.0 0.0 0.0 0.19885494515099864 0.0 31 0.0 0.0 0.0 0.22944801363576767 0.0 32 0.0 0.0 0.0 0.2950045889602727 0.0 33 0.0 0.0 0.0 0.34089419168742624 0.0 34 0.0 0.0 0.0 0.39770989030199727 0.0 35 0.0 0.0 0.0 0.48730387657882085 0.0 36 0.0 0.0 0.0 0.6380839998251825 0.0 37 0.0 0.0 0.0 0.8762728901708842 0.0 38 0.0 0.0 0.0 1.2040557667934093 0.0 39 0.0 0.0 0.0 1.5711725886106376 0.0 40 0.0 0.0 0.0 2.099995629561645 0.0 41 0.0 0.0 0.0 2.644115204755037 0.0 42 0.0 0.0 0.0 3.3193479305974387 0.0 43 0.0 0.0 0.0 3.9836545605524236 0.0 44 0.0 0.0 0.0 4.715702985009396 0.0 45 0.0 0.0 0.0 5.32319391634981 0.0 46 0.0 0.0 0.0 5.865128272365718 0.0 47 0.0 0.0 0.0 6.435470477688912 0.0 48 0.0 0.0 0.0 7.034220532319392 0.0 49 0.0 0.0 0.0 7.598007080110135 0.0 50 0.0 0.0 0.0 8.177090162143262 0.0 51 0.0 0.0 0.0 8.78676631266116 0.0 52 0.0 0.0 0.0 9.468554696036012 0.0 53 0.0 0.0 0.0 10.272715353349941 0.0 54 0.0 0.0 0.0 11.061579476421485 0.0 55 0.0 0.0 0.0 11.714960010489053 0.0 56 0.0 0.0 0.0 12.28748743498973 0.0 57 0.0 0.0 0.0 12.781346969101001 0.0 58 0.0 0.0 0.0 13.216205585420218 0.0 59 0.0 0.0 0.0 13.707879900354005 0.0 60 0.0 0.0 0.0 14.186442900222893 0.0 61 0.0 0.0 0.0 14.610375420654691 0.0 62 0.0 0.0 0.0 15.191643721865303 0.0 63 0.0 0.0 0.0 15.85157991346532 0.0 64 0.0 0.0 0.0 16.498404790000436 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCCGTT 15 0.0022119223 69.99454 11 CCTTATG 20 7.850691E-5 69.99454 3 GTTTACT 15 0.0022119223 69.99454 15 TAGCCGT 15 0.0022119223 69.99454 10 GCGCAGT 15 0.0022119223 69.99454 15 AGCGGGC 15 0.0022119223 69.99454 10 CGCTCTA 15 0.0022119223 69.99454 35 ATGAGCG 15 0.0022119223 69.99454 7 GAGCGGG 15 0.0022119223 69.99454 9 TCTAAGA 15 0.0022119223 69.99454 38 CGGGCGC 15 0.0022119223 69.99454 12 GCGGGCG 15 0.0022119223 69.99454 11 CAGTGAT 15 0.0022119223 69.99454 18 GTGAGCA 15 0.0022119223 69.99454 38 TGAGCGG 15 0.0022119223 69.99454 8 CGTTTAC 15 0.0022119223 69.99454 14 CCGTTTA 15 0.0022119223 69.99454 13 GCCGTTT 15 0.0022119223 69.99454 12 GCTTTCG 15 0.0022119223 69.99454 30 TGCCCTA 20 0.006911793 52.495903 52 >>END_MODULE