FastQCFastQC Report
Wed 25 May 2016
SRR1780508_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780508_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45762
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT29146.3677286831869235No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTT27946.105502381888903TruSeq Adapter, Index 5 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC9342.040994711769591No Hit
CTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC5001.0926095887417508No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG3570.78012324636161No Hit
TATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCT2490.5441195751933919No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGT1810.39552467112451384No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC1490.3255976574450417No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC1390.3037454656702067No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGTCTT1230.26878195883047074No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG1150.25130020541060266No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGTCTT1130.24692976705563566No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGT1010.22070713692583366No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC1000.21852191774835014No Hit
CTTAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC960.20978104103841613No Hit
CTTATAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG960.20978104103841613No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGT940.20541060268344916No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGTCTT810.1770027533761636No Hit
TTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC550.1201870547615926No Hit
CTTATCACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC550.1201870547615926No Hit
CTTATTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG510.11144617805165857No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCG150.00221123770.0000125
GAGACGC150.00221123770.0000124
AGCTGTG150.00221123770.0000169
GACGCGT150.00221123770.0000126
CGCGTGG150.00221123770.0000128
ACGCGTG150.00221123770.0000127
CTATACA650.059.2307661
CTTTACA1500.058.3333321
TTTACAC1550.056.451612
TTACACA1600.054.68753
ACCGAAA200.006909659652.512
GTAGATA200.006909659652.539
AGACAAA200.006909659652.570
GCGTGGT200.006909659652.529
CTTTATA200.006909659652.52
CTGATCA200.006909659652.533
GAACTCG350.00124317540.036
GATCAGG350.00124317540.01
AACTCGT350.00124317540.037
ATCGTAT556.559374E-638.18181639
Produced by FastQC (version 0.11.5)